Abstract:
OBJECTIVE:In this study, we compared the observed agreement and correlation of the Vitek 2 system with the biomarker-based MALDI-TOF MS identification results of bacteria and yeast on a routine basis. METHODS:Clinical isolates collected from two years were included. Isolates were identified using the Vitek 2 system and MALDI-TOF MS. The percent of observed agreements and the kappa coefficient (κ) with its corresponding 95% interval confidence were calculated between both results. When species-level biotyper identifications matched a member of a group, complex, or one of the species of a slashing call, the identification was considered correct for agreement calculations. RESULTS:The 4,238 recruited isolates included 2,669 gram-negative bacteria, 1,479 gram-positive bacteria, and 90 yeast. Among gram-negative bacteria, the most frequent species identified were Escherichia coli (κ=0.983), Acinetobacter baumannii complex (κ=0.979), Klebsiella pneumoniae (κ=0.972), and Pseudomonas aeruginosa (κ=0.970). Among Staphylococcal species, Staphylococcus aureus was the most frequently species detected (κ=0.986), followed by S. epidermidis (κ=0.904). For enterococcal species, Enterococcus faecalis (κ=0.882) and Enterococcus faecium (κ=0.849) were the most frequently detected. For yeasts, the more common species were Candida albicans (κ=0.888), followed by Candida tropicalis (κ=0.946) and Candida glabrata (κ=1.000). CONCLUSIONS:According to our results, when antimicrobial susceptibility tests are performed using Vitek 2 cards, the most common pathogens are correctly identified for the most frequent clinical isolates.
journal_name
Ann Clin Lab Scijournal_title
Annals of clinical and laboratory scienceauthors
Garza-González E,Camacho-Ortíz A,Rodríguez-Noriega E,Esparza-Ahumada S,Flores-Treviño S,Bocanegra-Ibarias P,Tijerina-Rodríguez L,Morfín-Otero Rsubject
Has Abstractpub_date
2020-01-01 00:00:00pages
119-127issue
1eissn
0091-7370issn
1550-8080pii
50/1/119journal_volume
50pub_type
杂志文章abstract::Neuropeptide modulation of hematopoietic stem cell differentiation was studied in an in vitro murine system. Endorphins were able to influence the erythropoietin-dependent differentiation of bone marrow cells into erythroid colony forming units in a dose dependent manner. The effects on progenitor cell maturation were...
journal_title:Annals of clinical and laboratory science
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abstract::This study compared the assay results of HBV markers obtained with two automated immunoassay systems in serum samples with concomitant HBV antigens and antibodies. Eighty-nine samples of concomitant HBsAg and anti-HBs and 74 samples of concomitant HBeAg and anti-HBe were analyzed by the Abbott Architect i2000 and the ...
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journal_title:Annals of clinical and laboratory science
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journal_title:Annals of clinical and laboratory science
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pub_type: 杂志文章
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journal_title:Annals of clinical and laboratory science
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doi:
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journal_title:Annals of clinical and laboratory science
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journal_title:Annals of clinical and laboratory science
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journal_title:Annals of clinical and laboratory science
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