Identification of an upstream activating sequence and an upstream repressible sequence of the pyruvate kinase gene of the yeast Saccharomyces cerevisiae.

abstract：:Inhibition of mitosis by antimitotic drugs is thought to occur by destruction of microtubules, causing cells to arrest through the action of one or more mitotic checkpoints. We have patterned experiments in the yeast Saccharomyces cerevisiae after recent studies in mammalian cells that demonstrate the effectiveness of...

journal_title：Molecular and cellular biology

authors： Wang Y,Burke DJ

更新日期：1995-12-01 00:00:00

abstract：:Posttranscriptional regulation is important for tumor necrosis factor alpha (TNF-alpha) expression in monocytes and macrophages, and an AU-rich element (ARE) in the 3' untranslated region (UTR) of TNF-alpha mRNA is implicated in control of its translation and mRNA stability. Regulation of mRNA turnover is thought to b...

journal_title：Molecular and cellular biology

authors： Dean JL,Wait R,Mahtani KR,Sully G,Clark AR,Saklatvala J

更新日期：2001-02-01 00:00:00

abstract：:We have identified stable transcripts from the so-called nontranscribed spacer region (NTS) of the nuclear ribosomal DNA repeat in certain respiration-deficient strains of Saccharomyces cerevisiae. These RNAs, which are transcribed from the same strand as is the 37S rRNA precursor, are 500 to 800 nucleotides long and ...

journal_title：Molecular and cellular biology

authors： Parikh VS,Conrad-Webb H,Docherty R,Butow RA

更新日期：1989-05-01 00:00:00

abstract：:The C-terminal domain of the RNA polymerase II largest subunit (the Rpb1 CTD) is composed of tandem heptad repeats of the consensus sequence Y(1)S(2)P(3)T(4)S(5)P(6)S(7). We reported previously that Thr 4 is phosphorylated and functions in histone mRNA 3'-end formation in chicken DT40 cells. Here, we have extended our...

journal_title：Molecular and cellular biology

authors： Hsin JP,Xiang K,Manley JL

更新日期：2014-07-01 00:00:00

abstract：:We reported the identification of a new family of DNA-binding proteins from our characterization of the dead ringer (dri) gene of Drosophila melanogaster. We show that dri encodes a nuclear protein that contains a sequence-specific DNA-binding domain that bears no similarity to known DNA-binding domains. A number of p...

journal_title：Molecular and cellular biology

authors： Gregory SL,Kortschak RD,Kalionis B,Saint R

更新日期：1996-03-01 00:00:00

abstract：:The pancreatic-islet-enriched transcription factors MafA and MafB have unique expression patterns in β cells in rodents. MafA is specifically expressed in β cells and is a key regulatory factor for maintaining adult β-cell function, whereas MafB plays an essential role in β-cell development during embryogenesis, and i...

journal_title：Molecular and cellular biology

authors： Xiafukaiti G,Maimaiti S,Ogata K,Kuno A,Kudo T,Shawki HH,Oishi H,Takahashi S

更新日期：2019-08-12 00:00:00

abstract：:The steroid hormone progesterone acts via high-affinity nuclear receptors that interact with specific DNA sequences located near the promoter of the hormone-responsive gene. Recent studies suggested that the hormone-occupied progesterone receptor (PR) mediates gene activation by recruiting a cellular coregulatory fact...

journal_title：Molecular and cellular biology

authors： Xu Y,Klein-Hitpass L,Bagchi MK

更新日期：2000-03-01 00:00:00

abstract：:Transfection experiments with constructs containing various 5'-deleted fragments of the human lipoprotein lipase (LPL) promoter and the chloramphenicol acetyltransferase reporter gene revealed an LPL silencer element (LSE) in the region of nucleotides -225 to -81 of the LPL gene that functioned in Chinese hamster ovar...

journal_title：Molecular and cellular biology

authors： Tanuma Y,Nakabayashi H,Esumi M,Endo H

更新日期：1995-01-01 00:00:00

abstract：:In vitro studies have implicated nuclear respiratory factor 1 (NRF-1) in the transcriptional expression of nuclear genes required for mitochondrial respiratory function, as well as for other fundamental cellular activities. We investigated here the in vivo function of NRF-1 in mammals by disrupting the gene in mice. A...

journal_title：Molecular and cellular biology

authors： Huo L,Scarpulla RC

更新日期：2001-01-01 00:00:00

abstract：:In Pichia pastoris, alcohol oxidase (AOX) is the first enzyme in the methanol utilization pathway and is encoded by two genes, AOX1 and AOX2. The DNA and predicted amino acid sequences of the protein-coding portions of the genes are closely homologous, whereas flanking sequences share no homology. The functional roles...

journal_title：Molecular and cellular biology

authors： Cregg JM,Madden KR,Barringer KJ,Thill GP,Stillman CA

更新日期：1989-03-01 00:00:00

abstract：:Genetic and biochemical evidence implicates chromatin structure in the silencing of the two quiescent mating-type loci near the telomeres of chromosome III in yeast. With high-resolution micrococcal nuclease mapping, we show that the HMRa locus has 12 precisely positioned nucleosomes spanning the distance between the ...

journal_title：Molecular and cellular biology

authors： Ravindra A,Weiss K,Simpson RT

更新日期：1999-12-01 00:00:00

abstract：:The O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins is dynamic and abundant in the nucleus and cytosol. Several transcription factors, including Sp1, have been shown to contain this modification; however, the functional role of O-GlcNAc in these proteins has not been determined. In this paper we descr...

journal_title：Molecular and cellular biology

authors： Roos MD,Su K,Baker JR,Kudlow JE

更新日期：1997-11-01 00:00:00

abstract：:Mammalian eukaryotic translation initiation factor 4F (eIF4F) is a cap-binding protein complex consisting of three subunits: eIF4E, eIF4A, and eIF4G. In yeast and plants, two related eIF4G species are encoded by two different genes. To date, however, only one functional eIF4G polypeptide, referred to here as eIF4GI, h...

journal_title：Molecular and cellular biology

authors： Gradi A,Imataka H,Svitkin YV,Rom E,Raught B,Morino S,Sonenberg N

更新日期：1998-01-01 00:00:00

abstract：:Tyrosine phosphorylation of CAS (Crk-associated substrate, p130(Cas)) has been implicated as a key signaling step in integrin control of normal cellular behaviors, including motility, proliferation, and survival. Aberrant CAS tyrosine phosphorylation may contribute to cell transformation by certain oncoproteins, inclu...

journal_title：Molecular and cellular biology

authors： Ruest PJ,Shin NY,Polte TR,Zhang X,Hanks SK

更新日期：2001-11-01 00:00:00

abstract：:The serum response factor (p67SRF) binds to a palindromic sequence in the c-fos serum response element (SRE). A second protein, p62TCF binds in conjunction with p67SRF to form a ternary complex, and it is through this complex that growth factor-induced transcriptional activation of c-fos is thought to take place. A 90...

journal_title：Molecular and cellular biology

authors： Sharrocks AD,Gille H,Shaw PE

更新日期：1993-01-01 00:00:00

abstract：:Regulated transcription of class II genes of the yeast Saccharomyces cerevisiae requires the diverse functions of mediator complex. In particular, MED6 is essential for activated transcription from many class II promoters, suggesting that it functions as a key player in the relay of activator signals to the basal tran...

journal_title：Molecular and cellular biology

authors： Lee YC,Kim YJ

更新日期：1998-09-01 00:00:00

abstract：:The homeo box gene even-skipped (eve) encodes a 376-amino-acid protein that binds with high affinity to sequences located near the 5' termini of the eve and en genes. The 5' en sites are A + T rich and contain copies of the 10-base-pair (bp) consensus sequence T-C-A-A-T-T-A-A-A-T. In contrast, the 5' eve sites are G +...

journal_title：Molecular and cellular biology

authors： Hoey T,Warrior R,Manak J,Levine M

更新日期：1988-11-01 00:00:00

abstract：:The ARK (AXL, UFO) receptor is a member of a new family of receptor tyrosine kinases whose extracellular domain contains a combination of fibronectin type III and immunoglobulin motifs similar to those found in many cell adhesion molecules. ARK mRNA is expressed at high levels in the mouse brain, prevalently in the hi...

journal_title：Molecular and cellular biology

authors： Bellosta P,Costa M,Lin DA,Basilico C

更新日期：1995-02-01 00:00:00

abstract：:The gene encoding the 180-kDa DNA strand transfer protein beta from the yeast Saccharomyces cerevisiae was identified and sequenced. This gene, DST2 (DNA strand transferase 2), was located on chromosome VII. dst2 gene disruption mutants exhibited temperature-sensitive sporulation and a 50% longer generation time durin...

journal_title：Molecular and cellular biology

authors： Dykstra CC,Kitada K,Clark AB,Hamatake RK,Sugino A

更新日期：1991-05-01 00:00:00

abstract：:RNase H1 in mammalian cells is present in nuclei and mitochondria. Its absence in mitochondria results in embryonic lethality due to the failure to amplify mitochondrial DNA (mtDNA). Dual localization to mitochondria and nuclei results from differential translation initiation at two in-frame AUGs (M1 and M27) of a sin...

journal_title：Molecular and cellular biology

authors： Suzuki Y,Holmes JB,Cerritelli SM,Sakhuja K,Minczuk M,Holt IJ,Crouch RJ

更新日期：2010-11-01 00:00:00

abstract：:Tetraspanins are clustered in specific microdomains (named tetraspanin-enriched microdomains, or TERM) in the plasma membrane and regulate the functions of associated transmembrane receptors, including integrins and receptor tyrosine kinases. We have identified syntenin-1, a PDZ domain-containing protein, as a new com...

journal_title：Molecular and cellular biology

authors： Latysheva N,Muratov G,Rajesh S,Padgett M,Hotchin NA,Overduin M,Berditchevski F

更新日期：2006-10-01 00:00:00

abstract：:Growth differentiation factor 15 (GDF15) is a secreted protein with pleotropic functions from the transforming growth factor β (TGF-β) family. GDF15 is synthesized as a precursor and undergoes proteolytic cleavage to generate mature GDF15. The strong appetite-suppressing effect of mature GDF15 makes it an attractive t...

journal_title：Molecular and cellular biology

authors： Li JJ,Liu J,Lupino K,Liu X,Zhang L,Pei L

更新日期：2018-10-15 00:00:00

abstract：:The activities of the related Abl and Arg nonreceptor tyrosine kinases are kept under tight control in cells, but exposure to several different stimuli results in a two- to fivefold stimulation of kinase activity. Following the breakdown of inhibitory intramolecular interactions, Abl activation requires phosphorylatio...

journal_title：Molecular and cellular biology

authors： Tanis KQ,Veach D,Duewel HS,Bornmann WG,Koleske AJ

更新日期：2003-06-01 00:00:00

abstract：:The simian virus 40 core origin of replication consists of nucleotides 5211 through 31. These 64 base pairs contain three functional domains with strict sequence requirements and two spacer regions with relaxed sequence specificity but precise positional constraints. The early domain extends for 10 contiguous base pai...

journal_title：Molecular and cellular biology

authors： Deb S,DeLucia AL,Baur CP,Koff A,Tegtmeyer P

更新日期：1986-05-01 00:00:00

abstract：:A new dominant amplifiable selective system for use in bacterium-animal cell shuttle vectors was developed by the insertion of a 2-kilobase genomic fragment containing the cloned Escherichia coli gene for asparagine synthetase (AS) into the pBR322-simian virus 40 recombinant vector pSV2 so as to place the translationa...

journal_title：Molecular and cellular biology

authors： Cartier M,Chang MW,Stanners CP

更新日期：1987-05-01 00:00:00

abstract：:The tumor suppressor p21 acts as a cell cycle inhibitor and has also been shown to regulate gene expression by functioning as a transcription corepressor. Here, we identified p21-regulated microRNAs (miRNAs) by sequencing small RNAs from isogenic p21(+/+) and p21(-/-) cells. Three abundant miRNA clusters, miR-200b-200...

journal_title：Molecular and cellular biology

authors： Li XL,Hara T,Choi Y,Subramanian M,Francis P,Bilke S,Walker RL,Pineda M,Zhu Y,Yang Y,Luo J,Wakefield LM,Brabletz T,Park BH,Sharma S,Chowdhury D,Meltzer PS,Lal A

更新日期：2014-02-01 00:00:00

abstract：:We generated a line of mice in which sterol regulatory element binding protein 1a (SREBP-1a) was specifically inactivated by insertional mutagenesis. Homozygous mutant mice were completely viable despite expressing SREBP-1a mRNA below 5% of normal, and there were minimal effects on expression of either SREBP-1c or -2....

journal_title：Molecular and cellular biology

authors： Im SS,Hammond LE,Yousef L,Nugas-Selby C,Shin DJ,Seo YK,Fong LG,Young SG,Osborne TF

更新日期：2009-09-01 00:00:00

abstract：:We have isolated a yeast conditional mutant which rapidly ceases synthesis of mRNA when subjected to the nonpermissive temperature. This mutant (rpb1-1) was constructed by replacing the wild-type chromosomal copy of the gene encoding the largest subunit of RNA polymerase II with one mutagenized in vitro. The rapid ces...

journal_title：Molecular and cellular biology

authors： Nonet M,Scafe C,Sexton J,Young R

更新日期：1987-05-01 00:00:00

abstract：:Double-stranded (ds) RNA-specific adenosine deaminase converts adenosine residues into inosines in dsRNA and edits transcripts of certain cellular and viral genes such as glutamate receptor (GluR) subunits and hepatitis delta antigen. The first member of this type of deaminase, DRADA1, has been recently cloned based o...

journal_title：Molecular and cellular biology

authors： Lai F,Chen CX,Carter KC,Nishikura K

更新日期：1997-05-01 00:00:00

abstract：:Mating-type genes resident in the silent cassette HML at the left arm of chromosome III are repressed by the action of four SIR gene products, most likely mediated through two cis-acting sites located on opposite sides of the locus. We showed that deletion of either of these two cis-acting sites from the chromosome di...

journal_title：Molecular and cellular biology

authors： Mahoney DJ,Broach JR

更新日期：1989-11-01 00:00:00