Anti-Salmonella Potential of New Lactobacillus Strains with the Application in the Poultry Industry.

Abstract:

:Probiotics are considered an alternative to antibiotics in the prevention and treatment of Salmonella diseases in poultry. However, to use probiotics as proposed above, it is necessary to evaluate their properties in detail and to select the most effective bacterial strains in the application targeted. In this study, probiotic properties of new Lactobacillus sp. strains were investigated and their antimicrobial activity against 125 environmental strains of Salmonella sp. was determined using the agar slab method. Furthermore, their survival in the presence of bile salts and at low pH, antibiotics susceptibility, aggregation and coaggregation ability, adherence to polystyrene and Caco-2 cells, and cytotoxicity were investigated. Each strain tested showed antagonistic activity against at least 96% of the environmental Salmonella sp. strains and thus representing a highly epidemiologically differentiated collection of poultry isolates. In addition, the probiotic properties of new Lactobacillus strains are promising. Therefore, all strains examined showed a high potential for use in poultry against salmonellosis. :Probiotics are considered an alternative to antibiotics in the prevention and treatment of Salmonella diseases in poultry. However, to use probiotics as proposed above, it is necessary to evaluate their properties in detail and to select the most effective bacterial strains in the application targeted. In this study, probiotic properties of new Lactobacillus sp. strains were investigated and their antimicrobial activity against 125 environmental strains of Salmonella sp. was determined using the agar slab method. Furthermore, their survival in the presence of bile salts and at low pH, antibiotics susceptibility, aggregation and coaggregation ability, adherence to polystyrene and Caco-2 cells, and cytotoxicity were investigated. Each strain tested showed antagonistic activity against at least 96% of the environmental Salmonella sp. strains and thus representing a highly epidemiologically differentiated collection of poultry isolates. In addition, the probiotic properties of new Lactobacillus strains are promising. Therefore, all strains examined showed a high potential for use in poultry against salmonellosis.

journal_name

Pol J Microbiol

authors

Kowalska JD,Nowak A,Śliżewska K,Stańczyk M,Łukasiak M,Dastych J

doi

10.33073/pjm-2020-001

subject

Has Abstract

pub_date

2020-09-01 00:00:00

pages

5-18

issue

1

eissn

1733-1331

issn

2544-4646

pii

exeley

journal_volume

69

pub_type

杂志文章
  • Bioactive Compounds of Pseudoalteromonas sp. IBRL PD4.8 Inhibit Growth of Fouling Bacteria and Attenuate Biofilms of Vibrio alginolyticus FB3.

    abstract::Biofouling is a phenomenon that describes the fouling organisms attached to man-made surfaces immersed in water over a period of time. It has emerged as a chronic problem to the oceanic industries, especially the shipping and aquaculture fields. The metal-containing coatings that have been used for many years to preve...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:10.21307/pjm-2019-003

    authors: Supardy NA,Ibrahim D,Mat Nor SR,Noordin WNM

    更新日期:2019-01-01 00:00:00

  • Antiviral Resistance of Splenocytes in Aged Mice.

    abstract::We compared the susceptibility to viral infection of splenocytes, isolated from young versus old CBA mice, and evaluated the antiviral actions of lactoferrin in splenocytes infected with Encephalomyocarditis virus (EMCV). Recombinant mouse lactoferrin (rmLF) and bovine lactoferrin (bLF) were used. There were no differ...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:10.5604/17331331.1235002

    authors: Zaczyńska E,Artym J,Kocięba M,Burster T,Kruzel M,Paprocka M,Zimecki M

    更新日期:2017-03-30 00:00:00

  • Inhibition of lactophage activity by quinolinilporphyrin and its zinc compex.

    abstract::The influence of free base of quinolinilporphyrin and its Zn complex on infectivity of lactophages E3, E5 and E17 has been studied. The results of our investigations show that inhibition of lactophage activity by Zn complex of quinolinilporphyrin at concentration 10 microM and 20 microM was in the range 53-62% and 65-...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Vodzinska N,Galkin B,Ishkov Y,Kirichenko A,Kondratyuk A,Filipova T

    更新日期:2011-01-01 00:00:00

  • Dependence of Colonization of the Large Intestine by Candida on the Treatment of Crohn's Disease.

    abstract::The aim of this study was to determine if there are quantitative differences in Candida fungi between pediatric patients with Crohn's disease (before and after exclusive enteral nutrition (EEN), and the biologic therapy with anti-tumor necrosis factor alpha - (IFX)), and healthy controls. DNA was isolated from fecal s...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:10.21307/pjm-2019-014

    authors: Kowalska-Duplaga K,Krawczyk A,Sroka-Oleksiak A,Salamon D,Wędrychowicz A,Fyderek K,Gosiewski T

    更新日期:2019-01-01 00:00:00

  • The Prevalence of Campylobacter spp. and Occurrence of Virulence Genes Isolated from Dogs.

    abstract::This study was conducted to determine the prevalence of Campylobacter spp. isolated from dogs' faecal samples. From June 2012 to June 2013, a total of 210 faecal samples from pet dogs living in different kennels (n = 210) were collected by the owners in Greater Poland Voivodeship, Poznań District, Poland. The study re...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Selwet M,Cłapa T,Galbas M,Słomski R,Porzucek F

    更新日期:2015-01-01 00:00:00

  • Metallo-Beta-Lactamase Producing Pseudomonas aeruginosa in a Healthcare Setting in Alexandria, Egypt.

    abstract::Pseudomonas aeruginosa has emerged as a major healthcare associated pathogen that creates a serious public health disaster in both developing and developed countries. In this work we aimed at studying the occurrence of metallo-beta-lactamase (MBL) producing P. aeruginosa in a healthcare setting in Alexandria, Egypt. T...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:10.5604/01.3001.0010.4855

    authors: Abaza AF,El Shazly SA,Selim HSA,Aly GSA

    更新日期:2017-09-27 00:00:00

  • Sonication as a diagnostic approach used to investigate the infectious etiology of prosthetic hip joint loosening.

    abstract::The purpose of the study was to evaluate the usefulness of sonication for the diagnosis of prosthetic joint infections (PJIs) by its comparison with periprosthetic tissues (PTs) and synovial fluid (SV-F) cultures. The study groups included 54 patients undergoing exchange of total hip prostheses for so called "aseptic"...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Bogut A,Niedźwiadek J,Kozioł-Montewka M,Strzelec-Nowak D,Blacha J,Mazurkiewicz T,Macias J,Marczyński W

    更新日期:2014-01-01 00:00:00

  • Toxoplasma gondii: usefulness of ROP1 recombinant antigen in an immunoglobulin G avidity assay for diagnosis of acute toxoplasmosis in humans.

    abstract::The results present in this study suggest that the Toxoplasma gondii recombinant ROP1 antigen in an IgG avidity test can be useful for detection of acute stage of infection. Specific antibodies of low avidity were detected in most of the sera from individuals with acute toxoplasmosis, while the absence or specific ant...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Holec-Gasior L,Drapała D,Lautenbach D,Kuri J

    更新日期:2010-01-01 00:00:00

  • Inorganic phosphorus and nitrogen modify composition and diversity of microbial communities in water of mesotrophic lake.

    abstract::The effects of inorganic nutrients (N, P) enrichment of mesotrophic lake water on changes in bacterial and protistan (heterotrophic nanoflagellates and ciliates) communities compositions were studied in the mesocosm experiment. Phosphorus (PO4(3-)) and nitrogen (NH4+) alone and in combination were added to three types...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Chróst RJ,Adamczewski T,Kalinowska K,Skowrońska A

    更新日期:2009-01-01 00:00:00

  • The cytotoxic and genotoxic effects of conjugated trans-2-nonenal (T2N), an off-flavor compound in beer and heat processed food arising from lipid oxidation.

    abstract::This study investigates the toxic effect of E(2)nonenal (trans-2-nonenal, T2N) and its conjugate with horse muscle myoglobin (Mb) tested on murine cell line L929 and human cell line A549, as well as the genotoxic effect of these compounds assayed by measuring of micronuclei in human cells K562. It is an aldehyde, whic...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Dey ES,Staniszewska M,Paściak M,Konopacka M,Rogoliński J,Gamian A,Danielsson B

    更新日期:2005-01-01 00:00:00

  • Methanogenic diversity studies within the rumen of Surti buffaloes based on methyl coenzyme M reductase A (mcrA) genes point to Methanobacteriales.

    abstract::Methane emissions from ruminant livestock are considered to be one of the more potent forms of greenhouse gases contributing to global warming. Many strategies to reduce emissions are targeting the methanogens that inhabit the rumen, but such an approach can only be successful if it targets all the major groups of rum...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Singh KM,Pandya PR,Parnerkar S,Tripathi AK,Ramani U,Koringa PG,Rank DN,Joshi CG,Kothari RK

    更新日期:2010-01-01 00:00:00

  • Development of a multiplex PCR (m-PCR) test for rapid identification of genes encoding heat-labile (LTI) and heat-stable (STI and STII) toxins of enterotoxigenic Escherichia coli (ETEC) with internal control of amplification.

    abstract::A multiplex PCR system was developed for specific identification of genes encoding heat-labile (LTI) and heat-stable (STI and STII) toxins of enterotoxigenic Escherichia coli (ETEC) strains. In addition, primers specific for the E. coli gene coding for 16S rRNA were used as an internal control of the DNA amplification...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Weiner M,Osek J

    更新日期:2004-01-01 00:00:00

  • Application of FLiP method for differentiation of Mycobacterium tuberculosis strains in comparison to commonly used methods, spoligotyping and MIRU-VNTR typing.

    abstract::The current "gold standard" in molecular epidemiological studies of Mycobacterium tuberculosis is IS6110 RFLP based on IS6110 polymorphism. However PCR-based methods are becoming increasingly important. Recently, fast ligation-mediated PCR (FLiP), based on IS6110 polymorphism was proposed. In this study, the discrimin...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Zaczek A,Brzostek A,Górna A,Sajduda A,Wojtasik A,Dziadek J

    更新日期:2013-01-01 00:00:00

  • Pathogenicity and ultrastructural studies of the mode of penetration by Phoma strasseri in peppermint stems and rhizomes.

    abstract::Pathogenicity and ultrastructural investigation of the inoculation of peppermint stems and rhizomes with Phoma strasseri conidia was undertaken using scanning and transmission electron microscopy to examine the host-parasite relationship. Pathogenicity experiments demonstrated that all tested P. strasseri isolates had...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Zimowska B

    更新日期:2012-01-01 00:00:00

  • Emerging linezolid-resistant, vancomycin resistant Enterococcus faecium from a patient of a haematological unit in Poland.

    abstract::The oxazolidinone antimicrobial, linezolid, has been approved for the treatment of infections caused by various gram-positive bacteria, including vancomycin-resistant enterococci (VRE). This is the first report on isolation of a linezolid resistant, vancomycin-resistant Enterococcus faecium (LRVREF) strain in Poland, ...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Krawczyk B,Samet A,Bronk M,Hellmann A,Kur J

    更新日期:2004-01-01 00:00:00

  • Assessment of molecular diversity in chickpea (Cicer arietinum L.) rhizobia and structural analysis of 16S rDNA sequences from Mesorhizobium ciceri.

    abstract::Molecular diversity studies of 19 rhizobia isolates from chickpea were conducted using simple sequence repeats (SSR) and 16S rDNA-RFLP markers. Phenotypic characterization with special reference to salinity and pH tolerance was performed. These isolates were identified as different strains of Mesorhizobium, Rhizobium,...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Yadav A,Singh AL,Rai GK,Singh M

    更新日期:2013-01-01 00:00:00

  • The Application of Impedance Microsensors for Real-Time Analysis of Pseudomonas aeruginosa Biofilm Formation.

    abstract::Biofilms formed by nosocomial pathogens represent a major threat to patients undergoing invasive procedures. As prophylaxis remains the most efficient anti-biofilm option, it is of paramount importance to develop diagnostic tools able to detect biofilm at the early stage of formation. The present study investigates th...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Chabowski K,Junka AF,Szymczyk P,Piasecki T,Sierakowski A,Mączynska B,Nitsch K

    更新日期:2015-01-01 00:00:00

  • Culturable Endophytes Diversity Isolated from Paeonia ostii and the Genetic Basis for Their Bioactivity.

    abstract::Paeonia ostii is known for its excellent medicinal values as Chinese traditional plant. To date, the diversity of culturable endophytes associated with P. ostii is in its initial phase of exploration. In this study, 56 endophytic bacteria and 51 endophytic fungi were isolated from P. ostii roots in China. Subsequent c...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:10.21307/pjm-2018-052

    authors: Yang RX,Zhang SW,Xue D,Xuan JH,Zhang YB,Peng BB

    更新日期:2018-01-01 00:00:00

  • Effect of ciprofloxacin and N-acetylcysteine on bacterial adherence and biofilm formation on ureteral stent surfaces.

    abstract::The aim of this study was to evaluate the effect of ciprofloxacin (CIP), N-acetylcysteine (NAC) alone and in combination on biofilm production and pre-formed mature biofilms on ureteral stent surfaces. Two strains each of Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Klebseilla pneumoniae, Pseud...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: El-Feky MA,El-Rehewy MS,Hassan MA,Abolella HA,Abd El-Baky RM,Gad GF

    更新日期:2009-01-01 00:00:00

  • First isolation of Clostridium difficile PCR-ribotype 027/toxinotype III in Poland.

    abstract::Of 175 Clostridium difficile strains isolated from patient hospitalized in one academic hospital in Warsaw between 2005-2006, one isolate belonged to PCR-ribotype 027/toxinotype III. This isolate had tcdA, tcdB, binary toxin genes (cdtA and cdtB), a 18-bp deletion and a 1 bp deletion at 117 position in the tcdC gene. ...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Pituch H,Bakker D,Kuijper E,Obuch-Woszczatyński P,Wultańska D,Nurzyńska G,Bielec A,Bar-Andziak E,Łuczak M

    更新日期:2008-01-01 00:00:00

  • Monitoring of Virulence Genes, Drug-Resistance in Campylobacter coli Isolated from Golden Retrievers.

    abstract::The investigation was performed on 75 of Golden Retriever puppies. Faecal samples were collected on the 42 day of the puppies life (con-trol). Probiotic preparation was administered on 43 day of the puppies life and 10 days after the application of the probiotic, faecal samples were collected again (on 53 day of puppi...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:10.5604/17331331.1204486

    authors: Selwet M,Galbas M,Słomski R,Cłapa T,Porzucek F

    更新日期:2016-06-07 00:00:00

  • Enzymatic Activity of Prototheca zopfii Strains Isolated from Cows with Mastitis.

    abstract::Bovine mastitis caused by Prototheca spp. can be a disease of high significance because of economic losses and the potential risk to public health. The aim of our study was to evaluate enzymatic activity of Prototheca zopfii. For this study, we used 15 P. zopfii strains previously isolated from cows with clinical and ...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Krukowski H,Lisowski A,Nowakowicz-Dębek B,Wlazło Ł

    更新日期:2012-09-28 00:00:00

  • Stable sulfur isotope fractionation by the green bacterium Chlorobaculum parvum during photolithoautotrophic growth on sulfide.

    abstract::Growing cultures of the green obligate photolithotroph, Chlorobaculum parvum DSM 263T (formerly Chlorobium vibrioforme forma specialis thiosulfatophilum NCIB 8327), oxidized sulfide quantitatively to elemental sulfur, with no sulfate formation. In the early stages of growth and sulfide oxidation, the sulfur product be...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Kelly DP

    更新日期:2008-01-01 00:00:00

  • Cytokine Levels in the In Vitro Response of T Cells to Planktonic and Biofilm Corynebacterium amycolatum.

    abstract::Unravelling of the interplay between the immune system and non-diphtheria corynebacteria would contribute to understanding their increasing role as medically important microorganisms. We aimed at the analysis of pro- (TNF, IL-1β, IL-6, IL-8, and IL-12p70) and anti-inflammatory (IL-10) cytokines produced by Jurkat T ce...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:10.33073/pjm-2019-045

    authors: Olender A,Bogut A,Magryś A,Tabarkiewicz J

    更新日期:2019-12-01 00:00:00

  • Could a step in the isolation of mycobacteria from sputum samples be eliminated by new decontamination kits?

    abstract::The efficacy of two mycobacterial homogenization, decontamination and concentration kits, Mycoprosafe and Decomics, were compared. A total of 146 sputum samples were examined in this study; 46 (31.5%) mycobacterial isolates were recovered with Mycoprosafe, while 39 (26.7%) mycobacterial isolates were recovered with De...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Karakeçe E,Terzi HA,Çiftci İH

    更新日期:2014-01-01 00:00:00

  • Molecular epidemiology of Salmonella enterica serovar Enteritidis strains by pulsed-field gel electrophoresis isolated in the Slovak Republic.

    abstract::A total 44 isolates of S. enterica serovar Enteritidis (S. Enteritidis) belonged to three different phage types (PTs) 9a, 13a, 25 were analyzed by the technique of pulsed-field gel electrophoresis (PFGE). Thirty and three strains were from two outbreaks (central and southern regions of the Slovak Republic PTs 13a, 25)...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Majtánová L,Szaboová M,Majtán V

    更新日期:2004-01-01 00:00:00

  • The occurrence, biodiversity and toxicity of Bacillus thuringiensis strains isolated from the insect pest Lymantria dispar (Poland).

    abstract::The aim of this investigation was to survey the occurrence, biodiversity, and toxicity of Bacillus thuringiensis strains originating from dead caterpillars of the forest pest, Lymantria dispar (Lepidoptera). Morphological, biochemical, and microscopic identification of isolates from the insects showed the presence of ...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Guz K,Bugla-Płoskońska G,Doroszkiewicz W

    更新日期:2009-01-01 00:00:00

  • Development of synbiotics with inulin, palatinose, alpha-cyclodextrin and probiotic bacteria.

    abstract::Success in creating a synbiotic depends on compatibility between the chosen components--prebiotic and probiotic. In this work the interactions between Lactobacillus sp. strains isolated from yogurts and type strains of Lactobacillus sp. and Lactococcus sp., and the dependence of their growth and antibacterial activity...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Pranckute R,Kaunietis A,Kuisiene N,Citavicius D

    更新日期:2014-01-01 00:00:00

  • Probable Interspecies Transfer of the bla(VIM-4) Gene between Enterobacter cloacae and Klebsiella pneumoniae in a Single Infant Patient.

    abstract::We report the interspecies transfer of the bla(VLM-4) gene in MBL-producing Enterobacter cloacae and Klebsiella pneumoniae isolates from a newborn patient who had received meropenem therapy. We show evidence that gene bla(VIM-4) was transmitted as a part of the class-1 integron on a ca. -90 kb conjugative plasmid. Hig...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:10.5604/17331331.1185239

    authors: Piekarska K,Zacharczuk K,Rzeczkowska M,Wołkowicz T,Januszkiewicz A,Podsiadły E,Demkow U,Gierczyński R

    更新日期:2015-01-01 00:00:00

  • Coomassie Blue G250 for Visualization of Active Bacteria from Lake Environment and Culture.

    abstract::Bacteria play a fundamental role in the cycling of nutrients in aquatic environments. A precise distinction between active and inactive bacteria is crucial for the description of this process. We have evaluated the usefulness of Coomassie Blue G250 for fluorescent staining of protein containing potentially highly acti...

    journal_title:Polish journal of microbiology

    pub_type: 杂志文章

    doi:10.5604/01.3001.0010.4867

    authors: Kiersztyn B,Siuda W,Chróst R

    更新日期:2017-09-27 00:00:00