Abstract:
:Clinical response to chimeric antigen receptor (CAR) T cell therapy is correlated with CAR T cell persistence, especially for CAR T cells that target CD19+ hematologic malignancies. 4-1BB-costimulated CAR (BBζ) T cells exhibit longer persistence after adoptive transfer than do CD28-costimulated CAR (28ζ) T cells. 4-1BB signaling improves T cell persistence even in the context of 28ζ CAR activation, which indicates distinct prosurvival signals mediated by the 4-1BB cytoplasmic domain. To specifically study signal transduction by CARs, we developed a cell-free, ligand-based activation and ex vivo culture system for CD19-specific CAR T cells. We observed greater ex vivo survival and subsequent expansion of BBζ CAR T cells when compared to 28ζ CAR T cells. We showed that only BBζ CARs activated noncanonical nuclear factor κB (ncNF-κB) signaling in T cells basally and that the anti-CD19 BBζ CAR further enhanced ncNF-κB signaling after ligand engagement. Reducing ncNF-κB signaling reduced the expansion and survival of anti-CD19 BBζ T cells and was associated with a substantial increase in the abundance of the most pro-apoptotic isoforms of Bim. Although our findings do not exclude the importance of other signaling differences between BBζ and 28ζ CARs, they demonstrate the necessary and nonredundant role of ncNF-κB signaling in promoting the survival of BBζ CAR T cells, which likely underlies the engraftment persistence observed with this CAR design.
journal_name
Sci Signaljournal_title
Science signalingauthors
Philipson BI,O'Connor RS,May MJ,June CH,Albelda SM,Milone MCdoi
10.1126/scisignal.aay8248subject
Has Abstractpub_date
2020-03-31 00:00:00issue
625eissn
1945-0877issn
1937-9145pii
13/625/eaay8248journal_volume
13pub_type
杂志文章abstract::GIV (Gα-interacting vesicle-associated protein; also known as Girdin) enhances Akt activation downstream of multiple growth factor- and G protein (heterotrimeric guanosine 5'-triphosphate-binding protein)-coupled receptors to trigger cell migration and cancer invasion. We demonstrate that GIV is a tyrosine phosphoprot...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.2002049
更新日期:2011-09-27 00:00:00
abstract::DNAX-activating protein of 12 kD (DAP12) is an immunoreceptor tyrosine-based activation motif (ITAM)-containing adaptor protein found in myeloid cells and natural killer cells, and it couples to various receptors that mediate either cellular activation or inhibition. DAP12 inhibits Toll-like receptor (TLR) signaling, ...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.2003801
更新日期:2013-08-20 00:00:00
abstract::For decades, the fission yeast Schizosaccharomyces pombe has been used as an excellent model with which to explore how cellular growth is coordinated with the division cycle, a yet-unanswered question in biology. New studies in this organism show that TOR (target of rapamycin) kinase and stress-responsive MAPK (mitoge...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.296pe74
更新日期:2009-11-10 00:00:00
abstract::Cancer-associated pain is debilitating. Understanding the mechanisms that cause it can inform drug development that may improve quality of life in patients. Here, we found that the reduced abundance of potassium channels called TRESK in dorsal root ganglion (DRG) neurons sensitized nociceptive sensory neurons and canc...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.aao5150
更新日期:2018-10-16 00:00:00
abstract::Regulatory factor X (Rfx) homologs regulate the transcription of genes necessary for ciliogenesis in invertebrates and vertebrates. Primary cilia are necessary for Hedgehog signaling and regulation of the activity of the transcriptional regulators known as Gli proteins, which are targets of Hedgehog signaling. Here, w...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.2000602
更新日期:2009-11-03 00:00:00
abstract::Cardiomyocytes contract against a mechanical load during each heartbeat, and excessive mechanical stress leads to heart diseases. Using a cell-in-gel system that imposes an afterload during cardiomyocyte contraction, we found that nitric oxide synthase (NOS) was involved in transducing mechanical load to alter Ca(2+) ...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.2005046
更新日期:2014-03-18 00:00:00
abstract::Educated natural killer (NK) cells have inhibitory receptors specific for self major histocompatibility complex (MHC) class I molecules and kill cancer cells more efficiently than do NK cells that do not have such receptors (hyporesponsive NK cells). The mechanism behind this functional empowerment through education h...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.aai9200
更新日期:2018-02-13 00:00:00
abstract::The cytoplasmic phosphatase PTPN22 (protein tyrosine phosphatase nonreceptor type 22) plays a key role in regulating lymphocyte homeostasis, which ensures that the total number of lymphocytes in the periphery remains relatively constant. Mutations in PTPN22 confer an increased risk of developing autoimmune diseases; h...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.2003365
更新日期:2012-11-27 00:00:00
abstract::MKK4 (mitogen-activated protein kinase kinase 4) is a pivotal upstream activator of c-Jun N-terminal kinase and p38. Here, we report that the abundance of MKK4 increases in senescent human diploid fibroblasts through enhanced translation. We identified four microRNAs (miR-15b, miR-24, miR-25, and miR-141) that target ...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.2000442
更新日期:2009-10-27 00:00:00
abstract::Alzheimer's disease (AD) is a progressive dementia disorder characterized by synaptic degeneration and amyloid-β (Aβ) accumulation in the brain. Through whole-genome sequencing of 1345 individuals from 410 families with late-onset AD (LOAD), we identified three highly penetrant variants in PRKCA, the gene that encodes...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.aaf6209
更新日期:2016-05-10 00:00:00
abstract::Ewing's sarcoma (ES) is a rare and highly malignant cancer that grows in the bones or surrounding tissues mostly affecting adolescents and young adults. A chimeric fusion between the RNA binding protein EWS and the ETS family transcription factor FLI1 (EWS-FLI1), which is generated from a chromosomal translocation, is...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.aam8429
更新日期:2017-10-03 00:00:00
abstract::Myeloproliferative neoplasms (MPNs) frequently have an activating mutation in the gene encoding Janus kinase 2 (JAK2). Thus, targeting the pathway mediated by JAK and its downstream substrate, signal transducer and activator of transcription (STAT), may yield clinical benefit for patients with MPNs containing the JAK2...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.2005301
更新日期:2014-12-23 00:00:00
abstract::The endoplasmic reticulum (ER) has emerged as a critical regulator of cell survival. IRE1 is a transmembrane protein with kinase and RNase activities that is localized to the ER and that promotes resistance to ER stress. We showed a mechanism by which IRE1 conferred protection against ER stress-mediated cell death. IR...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.aaa0341
更新日期:2015-06-23 00:00:00
abstract::Endothelial cell dysfunction, characterized by a diminished response to endothelial cell-dependent vasodilators, is a hallmark of hypertension. TRPV4 channels play a major role in endothelial-dependent vasodilation, a function mediated by local Ca(2+) influx through clusters of functionally coupled TRPV4 channels rath...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.2005052
更新日期:2014-07-08 00:00:00
abstract::The beta-amyloid (Abeta) peptide is associated with the pathogenesis of Alzheimer's disease (AD). Evidence gathered over the last two decades suggests that the gradual accumulation of soluble and insoluble Abeta peptide species triggers a cascade of events that leads to the clinical manifestation of AD. Abeta accumula...
journal_title:Science signaling
pub_type: 杂志文章,评审
doi:10.1126/scisignal.293re8
更新日期:2009-10-20 00:00:00
abstract::Under drought stress, abscisic acid (ABA) triggers closure of leaf cell pores called stomata, which are formed by two specialized cells called guard cells in plant epidermis. Two pathways downstream of ABA stimulate phosphorylation of the S-type anion channels SLAC1 (slow anion channel associated 1) and SLAH3 (SLAC1 h...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.2005703
更新日期:2014-09-09 00:00:00
abstract::Epigenetic modifications, such as DNA cytosine methylation, contribute to the mechanisms underlying learning and memory by coordinating adaptive gene expression and neuronal plasticity. Transcription-dependent plasticity regulated by DNA methylation includes synaptic plasticity and homeostatic synaptic scaling. Memory...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.aaf5642
更新日期:2016-08-23 00:00:00
abstract::The scattering of cultured epithelial cells in response to hepatocyte growth factor (HGF) is a model system that recapitulates key features of metastatic cell behavior in vitro, including disruption of cell-cell adhesions and induction of cell migration. We have developed image analysis tools that do not require fluor...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.2002677
更新日期:2012-07-03 00:00:00
abstract::The CXC chemokine CXCL12 mediates the chemoattraction of T cells and enhances the stimulation of T cells through the T cell receptor (TCR). The adaptor SLP-76 [Src homology 2 (SH2) domain-containing leukocyte protein of 76 kD] has two key tyrosine residues, Tyr(113) and Tyr(128), that mediate signaling downstream of t...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.2004018
更新日期:2013-07-30 00:00:00
abstract::Signaling pathways control cell fate decisions that ultimately determine the behavior of cancer cells. Therefore, the dynamics of pathway activity may contain prognostically relevant information different from that contained in the static nature of other types of biomarkers. To investigate this hypothesis, we characte...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.aab0990
更新日期:2015-12-22 00:00:00
abstract::The mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 2 (ERK2) is ubiquitously expressed in mammalian tissues and is involved in a wide range of biological processes. Although MAPKs have been intensely studied, identification of their substrates remains challenging. We have optimized a chem...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.2002010
更新日期:2011-10-25 00:00:00
abstract::The deubiquitylating enzyme OTUB1 is present in all tissues and targets many substrates, in both the cytosol and nucleus. We found that casein kinase 2 (CK2) phosphorylated OTUB1 at Ser(16) to promote its nuclear accumulation in cells. Pharmacological inhibition or genetic ablation of CK2 blocked the phosphorylation o...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.aaa0441
更新日期:2015-04-14 00:00:00
abstract::Transforming growth factor-β (TGF-β) is a pluripotent cytokine that regulates cell fate and plasticity in normal tissues and tumors. The multifunctional cellular responses evoked by TGF-β are mediated by the canonical SMAD pathway and by noncanonical pathways, including mitogen-activated protein kinase (MAPK) pathways...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.aal4186
更新日期:2017-07-04 00:00:00
abstract::The interplay between calcium ion (Ca(2+)) and cyclic adenosine monophosphate (cAMP) signaling underlies crucial aspects of cell homeostasis. The membrane-bound Ca(2+)-regulated adenylyl cyclases (ACs) are pivotal points of this integration. These enzymes display high selectivity for Ca(2+) entry arising from the acti...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.2002299
更新日期:2012-04-10 00:00:00
abstract::Regulatory T (T(reg)) cells are a unique CD4(+) T cell lineage that plays a crucial role in the maintenance of immunological tolerance. The Forkhead box transcription factor Foxp3 is critically involved in T(reg) cell development and responsible for determining the suppressive function of these cells. The majority of ...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.2001783
更新日期:2011-02-01 00:00:00
abstract::Intracellular calcium (Ca2+) signals are of prime importance for cellular function and behavior and are underpinned by a plethora of Ca2+ channels, pumps, transporters, and binding proteins that are regulated in complex ways. A series of biennial meetings, the International Meetings of the European Calcium Society (EC...
journal_title:Science signaling
pub_type:
doi:10.1126/scisignal.aaz0961
更新日期:2019-09-17 00:00:00
abstract::Defects in the biogenesis of or transport through primary cilia affect Hedgehog protein signaling, and many Hedgehog pathway components traffic through or accumulate in cilia. The Hedgehog receptor Patched negatively regulates the activity and ciliary accumulation of Smoothened, a seven-transmembrane protein that is e...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.aaa5622
更新日期:2015-06-02 00:00:00
abstract::T helper (T(H)) cells integrate biochemical cues present in the tissue microenvironment and produce cytokines that orchestrate immune responses. Previous discoveries have revealed a qualitative understanding of how T(H) cells process this biochemical information; however, the lack of methods to quantify how well these...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.2002657
更新日期:2012-04-17 00:00:00
abstract::The diacylglycerol kinases (DGKs) attenuate diacylglycerol (DAG)-mediated signals by catalyzing the conversion of DAG to phosphatidic acid. In T lymphocytes, the antigen-stimulated generation of DAG links signal strength to the intensity and duration of signaling by the Ras-extracellular signal-regulated kinase (ERK) ...
journal_title:Science signaling
pub_type: 杂志文章,评审
doi:10.1126/scisignal.aaa0974
更新日期:2015-04-28 00:00:00
abstract::During T cell activation by antigen-presenting cells (APCs), the diverse spatiotemporal organization of components of T cell signaling pathways modulates the efficiency of activation. Here, we found that loss of the tyrosine kinase interleukin-2 (IL-2)-inducible T cell kinase (Itk) in mice altered the spatiotemporal d...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.2001821
更新日期:2011-10-04 00:00:00