Overexpression of miR-455-5p affects retinol (vitamin A) absorption by downregulating STRA6 in a nitrofen-induced CDH with lung hypoplasia rat model.

Abstract:

:Lung hypoplasia is the main cause of congenital diaphragmatic hernia (CDH)-associated death but pathogenesis remains unclear. MiR-455-5p is involved in lung hypoplasia. We hypothesized that nitrofen causes abnormal miR-455-5p expression during lung development and designed this study to determine the relationship between miR-455-5p, stimulated by retinoic acid 6 (STRA6), and retinol in a nitrofen-induced CDH with lung hypoplasia rat model. Nitrofen or olive oil was administered to Sprague-Dawley rats by gavage on day 9.5 of gestation, and the rats were divided into a nitrofen group and a control group (n = 6). The left lung of fetuses was dissected on day 15.5. The expression of miR-455-5p or STRA6 messenger RNA (mRNA) was determined by quantitative real-time polymerase chain reaction. Average integrated optical density (IOD) of STRA6 protein was determined by immunofluorescence histochemistry. The average retinol level was detected by enzyme-linked immunosorbent assay (n = 6 lungs, respectively). Compared with the control group, the nitrofen group exhibited significantly increased miR-455-5p expression levels (29.450 ± 9.253 vs 5.955 ± 2.330; P = .00045) and significantly decreased STRA6 mRNA levels (0.197 ± 0.097 vs 0.588 ± 0.184; P = .0047). In addition, the average IOD of the STRA6 protein was significantly lower in the nitrofen group (805.643 ± 291.182 vs 1616.391 ± 572.308, P = .015), and the average retinol level was significantly reduced (4.013 ± 0.195 vs 5.317 ± 0.337 µg/L, P = .000). In summary, the overexpression of miR-455-5p affected retinol absorption by downregulating STRA6 in the nitrofen-induced CDH with lung hypoplasia rat model, and this downregulation may be one cause of CDH with lung hypoplasia.

journal_name

Pediatr Pulmonol

journal_title

Pediatric pulmonology

authors

Zheng J,He Q,Tang H,Li J,Xu H,Mao X,Liu G

doi

10.1002/ppul.24739

subject

Has Abstract

pub_date

2020-06-01 00:00:00

pages

1433-1439

issue

6

eissn

8755-6863

issn

1099-0496

journal_volume

55

pub_type

杂志文章
  • Quality of life in children with CF: Psychometrics and relations with stress and mealtime behaviors.

    abstract:BACKGROUND:The purpose of this study was to evaluate the utility of the Cystic Fibrosis Questionnaire-Revised (CFQ-R) with toddlers and preschool-aged children. Clinically relevant relations between health-related quality of life (HRQOL), stress, and mealtime behaviors have not been examined. It was hypothesized that p...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/ppul.23149

    authors: Driscoll KA,Modi AC,Filigno SS,Brannon EE,Chamberlin LA,Stark LJ,Powers SW

    更新日期:2015-06-01 00:00:00

  • Partitioning the respiratory effects of airway citric acid and normal saline in lambs.

    abstract::Aspiration is common in the intubated human neonate. Thus, the ventilatory and blood gas responses to citric acid and saline instillation into different airway sites were studied in ten awake, unanesthetised lambs, breathing spontaneously via a tracheostomy tube. With a system of balloons, 1 ml of saline or citric aci...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/ppul.1950030112

    authors: Hutchison AA,Caton D,Thomas RG,Bucciarelli RL

    更新日期:1987-01-01 00:00:00

  • Lung function in hyper IgE syndrome.

    abstract::Pneumatoceles and bronchiectasis are secondary complications of respiratory infections in patients with hyper IgE syndrome (HIgES). We report on a patient with HIgES and recurrent pneumonias since 1 year of age, with progression to pneumatoceles and bronchiectasis and fatal outcome, with disseminated infection and sus...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/ppul.22532

    authors: Roxo P Jr,Torres LA,Menezes UP,Melo JM

    更新日期:2013-01-01 00:00:00

  • Effect of cumulative oxygen exposure on respiratory symptoms during infancy among VLBW infants without bronchopulmonary dysplasia.

    abstract:UNLABELLED:Very low birth weight (VLBW) infants, even those without bronchopulmonary dysplasia (BPD) are at risk for pulmonary morbidity during infancy. Although some studies have found an association between the level of neonatal oxygen exposure and later morbidity, others have not. A possible explanation for these in...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/ppul.21199

    authors: Stevens TP,Dylag A,Panthagani I,Pryhuber G,Halterman J

    更新日期:2010-04-01 00:00:00

  • Respiratory pattern at hospital discharge in asymptomatic preterm infants.

    abstract::Pneumogram (PG) recordings were performed in 87 very low birthweight (VLBW) asymptomatic infants just prior to hospital discharge to determine the relationships between: 1) a prior history of apnea of prematurity (AOP) and cardiorespiratory pattern; and 2) cardiorespiratory pattern at hospital discharge and postconcep...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/ppul.1950040204

    authors: Hageman JR,Holmes D,Suchy S,Hunt CE

    更新日期:1988-01-01 00:00:00

  • Congenital lung abnormality quantification by computed tomography: The CLAQ method.

    abstract:INTRODUCTION:To date, no consensus has been reached on the optimal management of congenital lung abnormalities, and factors predicting postnatal outcome have not been identified. We developed an objective quantitative computed tomography (CT) scoring method, and assessed its value for clinical decision-making. METHODS...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/ppul.25032

    authors: Hermelijn SM,Dragt OV,Bosch JJ,Hijkoop A,Riera L,Ciet P,Wijnen RMH,Schnater JM,Tiddens HAWM

    更新日期:2020-11-01 00:00:00

  • A decade's experience of pediatric lung abscess and empyema at a community hospital.

    abstract:BACKGROUND:Discussions on the diagnostic and management of acquired pediatric lung pathology are usually published by large tertiary children's hospitals. It is likely that much of this pathology is actually seen and managed in nonacademic practices. METHODS:A 10-year retrospective review of patients under 18-years of...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/ppul.25254

    authors: Kanitra JJ,Thampy CA,Cullen ML

    更新日期:2021-01-02 00:00:00

  • Magnetic resonance imaging of the larynx in the pediatric population: A systematic review.

    abstract:BACKGROUND:Magnetic Resonance Imaging (MRI) techniques to image the larynx have evolved rapidly into a promising and safe imaging modality, without need for sedation or ionizing radiation. MRI is therefore of great interest to image pediatric laryngeal diseases. Our aim was to review MRI developments relevant for the p...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/ppul.24250

    authors: Elders BBLJ,Hermelijn SM,Tiddens HAWM,Pullens B,Wielopolski PA,Ciet P

    更新日期:2019-04-01 00:00:00

  • A case of pulmonary artery sling where onset Was induced by therapeutic hypothermia.

    abstract::A neonate with severe neonatal asphyxia was treated with therapeutic hypothermia. He developed hypothermia-induced respiratory deterioration, after which congenital tracheal stenosis and pulmonary artery sling were diagnosed. Even low-grade hypothermia is likely to induce bronchial narrowing in neonates, especially in...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/ppul.23538

    authors: Nakajima J,Sunohara D,Kawashima H

    更新日期:2017-03-01 00:00:00

  • Ultrastructural ciliary defects in children with recurrent infections of the lower respiratory tract.

    abstract::One hundred fifty-four children with recurrent or chronic infections of the lower respiratory tract compatible with the diagnosis of primary ciliary dyskinesia (PCD) were evaluated for the presence of ultrastructural ciliary abnormalities. Studies were performed on multiple samples of respiratory mucosa obtained by na...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/ppul.1950100104

    authors: Barlocco EG,Valletta EA,Canciani M,Lungarella G,Gardi C,De Santi MM,Mastella G

    更新日期:1991-01-01 00:00:00

  • Dynamic responses to tube breathing during the first 10 days of life.

    abstract::To assess whether there is a maturation of the response to tube breathing during the first 10 days of life, we have measured the dynamic ventilatory responses of nine term infants (34 studies) to added tubes equivalent to two anatomical dead spaces. Such a method allows an assessment of the baby's ability to increase ...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/ppul.1950090203

    authors: Upton CJ,Milner AD,Stokes GM,Wilson AJ

    更新日期:1990-01-01 00:00:00

  • Treatment of anorexia and weight loss with megestrol acetate in patients with cystic fibrosis.

    abstract::Four patients with severe cystic fibrosis lung disease, anorexia and weight loss, received Megestrol Acetate (MA), as an appetite stimulant. The initial dose was 400-800 mg daily and was continued for 6-15 months. Appetite was improved, with significant weight gain in all patients and an increase in their weight for a...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/(sici)1099-0496(199911)28:5<380::aid-ppul1

    authors: Nasr SZ,Hurwitz ME,Brown RW,Elghoroury M,Rosen D

    更新日期:1999-11-01 00:00:00

  • Standardized procedure for measurement of nasal potential difference: an outcome measure in multicenter cystic fibrosis clinical trials.

    abstract::Patients with cystic fibrosis (CF) can be discriminated from healthy subjects by measurement of the nasal potential difference, which has become a useful outcome measure for therapies directed toward correcting defective electrolyte transport in CF. A standard operating procedure was developed by a CF Foundation clini...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章,多中心研究

    doi:10.1002/ppul.10448

    authors: Standaert TA,Boitano L,Emerson J,Milgram LJ,Konstan MW,Hunter J,Berclaz PY,Brass L,Zeitlin PL,Hammond K,Davies Z,Foy C,Noone PG,Knowles MR

    更新日期:2004-05-01 00:00:00

  • Radiographic findings among children hospitalized with severe community-acquired pneumonia.

    abstract:BACKGROUND:Community-acquired pneumonia (CAP) is a leading cause of childhood death. There are few published reports of radiographic findings among children with severe CAP. OBJECTIVE:To describe chest X-ray (CXR) findings and assess association between these radiographic findings and pneumococcal isolation in childre...

    journal_title:Pediatric pulmonology

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1002/ppul.21287

    authors: Ferrero F,Nascimento-Carvalho CM,Cardoso MR,Camargos P,March MF,Berezin E,Ruvinsky R,Sant'Anna C,Feris-Iglesias J,Maggi R,Benguigui Y,CARIBE group.

    更新日期:2010-10-01 00:00:00

  • Obstructive sleep apnea in infancy: a 7-year experience at a pediatric sleep center.

    abstract:PURPOSE:To investigate the common indications for polysomnogram (PSG) associated co-morbid conditions, evaluation strategies, treatment options, and outcomes in a series of infants diagnosed with obstructive sleep apnea (OSA) by a PSG. METHODS:Retrospective chart review of infants who underwent PSG over a 7-year perio...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/ppul.22867

    authors: Ramgopal S,Kothare SV,Rana M,Singh K,Khatwa U

    更新日期:2014-06-01 00:00:00

  • Corticosteroid treatment for pulmonary lymphoid hyperplasia in children with the acquired immune deficiency syndrome.

    abstract::Five children with positive serology for human immunodeficiency virus (HIV) infection by enzyme-linked immunosorbent assay and Western blot were followed for chronic pulmonary disease. Lung biopsies were performed in all patients, and confirmed the diagnosis of pulmonary lymphoid hyperplasia. All children demonstrated...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/ppul.1950040105

    authors: Rubinstein A,Bernstein LJ,Charytan M,Krieger BZ,Ziprkowski M

    更新日期:1988-01-01 00:00:00

  • Prevalence of respiratory syncytial virus infection in Italian infants hospitalized for acute lower respiratory tract infections, and association between respiratory syncytial virus infection risk factors and disease severity.

    abstract::This study was designed to collect data on the prevalence of respiratory syncytial virus (RSV) infection in Italy in infants hospitalized for lower respiratory tract infections, and to evaluate which of the recognized risk factors might be associated with disease severity. Thirty-two centers throughout Italy participa...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章,多中心研究

    doi:10.1002/ppul.10047

    authors: Lanari M,Giovannini M,Giuffré L,Marini A,Rondini G,Rossi GA,Merolla R,Zuccotti GV,Salvioli GP,Investigators R.A.DA.R. Study Group.

    更新日期:2002-06-01 00:00:00

  • Are lower airway or throat cultures predictive of sinus bacteriology in cystic fibrosis?

    abstract::The choice of antibiotics for sinusitis in children with cystic fibrosis (CF) is empirical or based on lower airway cultures, because sinus cultures are difficult to obtain. The aim of this study was to identify the main organisms cultured from CF children with chronic sinusitis, and to evaluate the concordance of con...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/ppul.20396

    authors: Muhlebach MS,Miller MB,Moore C,Wedd JP,Drake AF,Leigh MW

    更新日期:2006-05-01 00:00:00

  • Reference equations for the interpretation of forced expiratory and plethysmographic measurements in infants.

    abstract:BACKGROUND:Pulmonary function testing is commonly performed for diagnosis and clinical management of respiratory diseases. It is important to use appropriate reference equations from healthy subjects for interpretation of data from infants with lung disease. This study aimed to determine if published reference equation...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/ppul.24063

    authors: Lu Z,Foong RE,Kowalik K,Moraes TJ,Dubeau A,Lefebvre D,Davis SD,Balkovec S,Becker A,Mandhane P,Turvey SE,Lou W,Sears MR,Ratjen F,Subbarao P

    更新日期:2018-07-01 00:00:00

  • Effectiveness and tolerability of high-dose salmeterol in cystic fibrosis.

    abstract::The efficacy and tolerability of high-dose salmeterol (100 mcg, BID) and albuterol (2.5 mg, BID) were compared with those of albuterol (2.5 mg, BID) in outpatients with cystic fibrosis in a randomized, double-blind, double-dummy, placebo-controlled, crossover study with both short- (4 weeks of each) and long-term (24 ...

    journal_title:Pediatric pulmonology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1002/ppul.10162

    authors: Hordvik NL,Sammut PH,Judy CG,Colombo JL

    更新日期:2002-10-01 00:00:00

  • Validation of the pediatric sleep questionnaire in children with asthma.

    abstract:OBJECTIVE:The prevalence of obstructive sleep apnea (OSA) is higher in children with poorly controlled asthma. We aimed to determine the validity of the Pediatric Sleep Questionnaire (PSQ) to screen for OSA in children with asthma. METHODS:This retrospective review encompassed sleep studies and medical records of asth...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/ppul.23568

    authors: Ehsan Z,Kercsmar CM,Collins J,Simakajornboon N

    更新日期:2017-03-01 00:00:00

  • Familial susceptibility to severe respiratory infection in early life.

    abstract::Lower respiratory tract infections (LRTI) are common in the first year of life and are mostly caused by viruses. Severity of LRTI in infants is associated with early-life environmental factors. Genetic association studies also suggest a role of heredity in susceptibility to acute bronchiolitis. We designed a case cont...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/ppul.20069

    authors: Goetghebuer T,Kwiatkowski D,Thomson A,Hull J

    更新日期:2004-10-01 00:00:00

  • Impact of home spirometry on medication adherence among adolescents with cystic fibrosis.

    abstract:OBJECTIVE:Medication adherence among adolescents with cystic fibrosis (CF) is often suboptimal and this has significant impact on their health and quality of life. The purpose of the study was to evaluate the impact of frequent home pulmonary function (PFT) monitoring on medication adherence among adolescents with CF. ...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/ppul.23950

    authors: Shakkottai A,Kaciroti N,Kasmikha L,Nasr SZ

    更新日期:2018-04-01 00:00:00

  • Development of the pulmonary airways in the fetal rat and its relation to the prenatal environment.

    abstract::We studied the left lung using multi-focus microphotography in 378 rat fetuses, assessing airway branching from day 13 to day 19 of gestation, and lung growth variables from day 13 to day 21. Longitudinal growth, and monopodial and dichotomous branching brought about a consistent airway pattern with variations within ...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1099-0496(199604)21:4<219::AID-PPUL3

    authors: Rotschild A,Massoud EA,Solimano A,Puterman ML,Sekhon HS,Thurlbeck WM

    更新日期:1996-04-01 00:00:00

  • Pulmonary hypertension in the premature infant population: Analysis of echocardiographic findings and biomarkers.

    abstract:OBJECTIVE:Extremely low gestational age neonates (ELGANs) are at risk for pulmonary hypertension (PH). We hypothesized that PH, defined by echocardiogram at 36 weeks gestational age (GA), would associate with respiratory morbidity, increased oxidant stress, and reduced nitric oxide production. STUDY DESIGN:ELGANs in t...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/ppul.23913

    authors: O'Connor MG,Suthar D,Vera K,Slaughter JC,Maitre NL,Steele S,Beller A,Fike CD,Aschner JL,Moore PE,Austin ED

    更新日期:2018-03-01 00:00:00

  • Pulmonary dysfunction after primary closure of an abdominal wall defect and its improvement with bronchodilators.

    abstract::To determine the extent of pulmonary dysfunction following primary closure of an abdominal wall defect, we obtained pulmonary function tests (PFT) in 11 newborn infants with gastroschisis and 6 with large omphaloceles admitted to a newborn ICU in a children's hospital. Patients were 1 to 30 days of age at the time of ...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/ppul.1950120309

    authors: Nakayama DK,Mutich R,Motoyama EK

    更新日期:1992-03-01 00:00:00

  • Short-term efficacy of ultrasonically nebulized hypertonic saline in cystic fibrosis.

    abstract::Progressive lung disease in patients with cystic fibrosis (CF) is caused by thick secretions, which cause airway obstruction and subsequent colonization and infection by inhaled pathogenic microorganisms. Recently, recombinant human DNase has been shown to reduce the viscoelasticity of sputum in patients with cystic f...

    journal_title:Pediatric pulmonology

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验

    doi:10.1002/(SICI)1099-0496(199602)21:2<77::AID-PPUL3>

    authors: Eng PA,Morton J,Douglass JA,Riedler J,Wilson J,Robertson CF

    更新日期:1996-02-01 00:00:00

  • Necrotizing sarcoid granulomatosis: a rarity in childhood.

    abstract::Necrotizing sarcoid granulomatosis (NSG) is characterized by pulmonary nodular infiltrates, a typical histology, and a benign clinical course. The etiology and pathogenesis of the disease are still unknown. In childhood, it is extremely rare, with only three reported cases so far. Here we report on an 8-year-old girl,...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/ppul.10242

    authors: Heinrich D,Gordjani N,Trusen A,Marx A,Hebestreit H

    更新日期:2003-05-01 00:00:00

  • Risk factors for fixed airflow obstruction in children and adolescents with asthma: 4-Year follow-up.

    abstract:BACKGROUND:Asthma is a disease with reversible bronchoconstriction; however, some patients develop fixed airflow obstruction (FAO). Previous studies have reported the incidence and risk factors of FAO in adults; however, the corresponding factors in children remain poorly understood. AIM:To evaluate the incidence and ...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/ppul.24625

    authors: Sousa AW,Barros Cabral AL,Arruda Martins M,Carvalho CRF

    更新日期:2020-03-01 00:00:00

  • Reference values for spirometric parameters in healthy children living in a Colombian city located at 2640 m altitude.

    abstract:OBJECTIVES:To identify the spirometric equations that are most appropriate for use in children and adolescents living in Bogota, Colombia after evaluating a set of relevant previously-developed equations, including the Global Lung Function Initiative (GLI) 2012 spirometry reference equations. METHODS:Healthy children ...

    journal_title:Pediatric pulmonology

    pub_type: 杂志文章

    doi:10.1002/ppul.24331

    authors: Aristizabal-Duque R,Castiblanco E,Rodriguez I,Sossa-Briceño MP,Rodriguez-Martinez CE

    更新日期:2019-06-01 00:00:00