Abstract：
:A large-scale isolation of islets is required for islet transplantation. We improved our conventional method, and could obtain about three times more islets than by the conventional methods. Pancreata of adult Wistar rats were inflated by injection of buffer with (A) or without 1.3 mg/ml collagenase (B). The rats were bled from the inferior vena cava and the aorta in (A) simultaneously with the inflation. They were further digested with collagenase and filtered through two different meshes (pore size: 1190 and 590 microns) (A1) or three different meshes (pore size: 1190, 590 120 microns) (A2) in order. Insulin released from islets isolated in this manner was determined by 1-h incubation with 3.3 and 16.7 mM glucose. Besides, 600 islets each were transplanted into the liver of streptozotocin-induced diabetic Wistar rats and their fasting plasma glucose was measured at weekly intervals. (1) With these methods more numerous islets were harvested by A1 (mean: 554) and A2 (mean: 746) than B (mean: 224). (2) Insulin released at both glucose concentrations was similar among islets obtained by A1, A2 and B. (3) The plasma glucose-lowering effect was similar among the islets obtained by these methods. (4) A more selected range of islet sizes was obtained by A2 than A1. These observations indicate that the present techniques (A1 and A2) are less time-consuming and simpler for a large-scale isolation of islets.
journal_name
Diabetes Res Clin Practjournal_title
Diabetes research and clinical practiceauthors
Hara Y,Taniguchi H,Ishihara K,Ejiri K,Tsutou A,Narutaki K,Baba Sdoi
10.1016/0168-8227(89)90113-7subject
Has Abstractpub_date
1989-02-15 00:00:00pages
103-8issue
2eissn
0168-8227issn
1872-8227pii
0168-8227(89)90113-7journal_volume
6pub_type
杂志文章abstract：:We have studied the association between heart rate and glycemia in 221 diabetic and 109 non-diabetic adult subjects. Supine and standing heart rates were both statistically significantly higher (P less than 0.001) in the diabetic patients than in the control subjects. Among the diabetics, both the supine and standing ...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/s0168-8227(88)80074-3
更新日期：1988-05-19 00:00:00
abstract：:Patients with diabetes mellitus have a higher rate of mortality than the general population. This higher mortality may be attributed mainly to cardiovascular disease. A high prevalence of dyslipidemia in diabetics can be one of the reasons for this. The most commonly recognized lipid abnormality in non-insulin-depende...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,评审
doi：10.1016/0168-8227(96)01263-6
更新日期：1996-06-01 00:00:00
abstract：AIMS:To investigate the relationship of coronary artery calcium (CAC) scores with common carotid artery intima media thickness (CCA-IMT), albuminuria and inflammatory factors in type 2 diabetes. METHODS AND RESULTS:128 asymptomatic type 2 diabetic patients, with at least one cardiovascular risk factor in addition to d...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2012.04.012
更新日期：2012-10-01 00:00:00
abstract：:A low-carbohydrate/high-fat evening meal impaired the glucose tolerance of healthy subjects to almost the same extent as the impairment caused by overnight fasting. This suggests that the decreased component (carbohydrate) is more relevant than the increased component (fat) to the impairment of glucose tolerance by a ...
journal_title：Diabetes research and clinical practice
pub_type： 信件
doi：10.1016/s0168-8227(01)00291-1
更新日期：2002-01-01 00:00:00
abstract：AIMS:To compare the feasibility of high intensity interval exercise (HI-IE) versus moderate intensity continuous exercise (MI-CE) in patients with type 2 diabetes (T2D), and to investigate the preliminary efficacy of HI-IE and MI-CE for improving glycated hemoglobin A1c (HbA1c) and body composition. METHODS:Individual...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.diabres.2012.10.019
更新日期：2013-02-01 00:00:00
abstract：AIMS:To explore associations between IRS-1 rs1801278 G>A polymorphism and metabolic syndrome (MS). METHODS:rs1801278 G>A was genotyped in 610 healthy Argentinian men. RESULTS:GA carriers had lower risk of MS (OR=0.52, P=0.045), particularly among smokers (OR=0.10, P=0.006). CONCLUSIONS:rs1801278 GA carriers had lowe...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2011.05.018
更新日期：2011-09-01 00:00:00
abstract：:Amputation has been known to be a rare adverse event of sodium glucose co-transporter-2 (SGLT2) inhibitors. It remains unclear whether the SGLT2 inhibitor as a class or specific categories of the SGLT2 inhibitors are linked with an increased risk of amputation. The objective of this meta-analysis was to investigate th...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,meta分析
doi：10.1016/j.diabres.2020.108136
更新日期：2020-05-01 00:00:00
abstract：:Prediabetes (IPD; n=122) and normoglycemic individuals (n=100) underwent assessment of polymorphisms of TNFα (-238, -308) and IL6 (-174). After 27.25±5.64 months, 16 IPD had reverted to normoglycemia and 18 progressed to diabetes. TNFα -238AA/GA genotypes were significantly more common in IPD, had higher TNFα, higher ...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2012.12.007
更新日期：2013-03-01 00:00:00
abstract：:Cultured cells derived from hamster insulinoma (In-111 R1 cells) were placed in 1.4 M dimethyl sulfoxide (Me2SO)-containing RPMI 1640 at 20 degrees C for 20 min. They were frozen to -40 degrees C at a cooling rate of 1.0 or 0.5 degrees C/min, subsequently to -80 degrees C at 3 degrees C/min with a programmable freezer...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/0168-8227(91)90097-w
更新日期：1991-02-01 00:00:00
abstract：AIM:To assess efficacy/safety of initial linagliptin/metformin single-pill combination (SPC) therapies versus individual drug components over 24weeks in treatment-naïve Asian patients with type 2 diabetes mellitus and insufficient glycemic control. METHODS:Patients (initial glycated hemoglobin [HbA1c] ⩾7.5% to <11.0% ...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.diabres.2016.11.026
更新日期：2017-02-01 00:00:00
abstract：AIMS:Myopia may have protective effects against diabetic retinopathy (DR). However, the data from epidemiologic studies are inconsistent. We aimed to examine the association between myopia and DR by conducting a meta-analysis. METHODS:We identified studies by searching the PubMed and EMBASE databases. Study-specific o...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.diabres.2015.10.020
更新日期：2016-01-01 00:00:00
abstract：:Glucagon-like peptide-1 (GLP-1) (1-37) and the fraction derived from it, GLP-1 (7-36 amide), are peptides encoded by the preproglucagon gene and possibly co-secreted with enteroglucagon. When added at a 25-nM concentration, GLP-1 (7-36 amide) decreased the release of glucagon from the perfused rat pancreas from 68.5 +...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/s0168-8227(88)80063-9
更新日期：1988-10-14 00:00:00
abstract：AIMS:This study was designed to compare the risk of long-term health outcomes, including microvascular, macrovascular complications and mortality, across 4 cohorts: triple-goal, dual-goal, single-goal, and no-goal achievers. METHODS:A retrospective cohort of 53,120 patients with T2DM were identified (97.51% male, 61.4...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2018.02.013
更新日期：2018-06-01 00:00:00
abstract：AIM:Whether exposure to relatively high levels of air pollution is associated with diabetes occurrence remains unclear. We sought to assess and quantify the association between exposure to major air pollutants and risk of type 2 diabetes. METHODS:PubMed and EMBASE databases (through September 2013) were searched using...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.diabres.2014.08.010
更新日期：2014-11-01 00:00:00
abstract：AIM:To examine the effect of oral diabetes medication on the risk of dementia in an elderly cohort with type 2 diabetes. METHODS:This was a population-based cohort study using the Korean National Health Insurance claims data from 2002 to 2013. Elderly subjects (60 years of age or older) with and without type 2 diabete...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2019.07.004
更新日期：2019-08-01 00:00:00
abstract：AIM:Oxytocin regulates food intake, carbohydrate and lipid metabolism, and urinary sodium excretion. We assessed the association between serum oxytocin levels and presence of metabolic syndrome (MetS) in older men. METHODS:Cross-sectional study was performed in 540 volunteer men aged 50-85yrs from the MINOS cohort. Ox...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2016.09.022
更新日期：2016-12-01 00:00:00
abstract：AIMS:To examine the relationship of non-high-density lipoprotein cholesterol (non-HDL-C) level with cardiovascular disease (CVD) risk in type 2 diabetes patients and the general population by conducting a meta-analysis. METHODS:We made a comprehensive literature search for relevant observational studies investigating ...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,meta分析
doi：10.1016/j.diabres.2018.11.002
更新日期：2019-01-01 00:00:00
abstract：AIMS:Accelerated atherosclerosis is the major cause of mortality in diabetic patients and increased oxidative stress probably plays an important role in its development. The aim of our study was to evaluate the relationship between thioredoxin-interacting protein (TXNIP) as an oxidative stress parameter and carotid art...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2015.05.028
更新日期：2015-08-01 00:00:00
abstract：AIMS:The objective of this study was to evaluate the prospective associations between type 2 diabetes mellitus (T2DM) and the risk of periodontitis and tooth loss. METHODS:35,247 male participants of the Health Professionals Follow-Up Study who were dentate, free of periodontitis and cancer at baseline, were followed ...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2012.09.039
更新日期：2012-12-01 00:00:00
abstract：AIM:The increased incidence of type 2 diabetes mellitus (T2DM) and the importance of early identification and management of its complications, especially diabetic nephropathy (DN), have spotted the light on genetic factors that increase risk of T2DM and its related nephropathy. The present study aimed at investigating ...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2018.03.053
更新日期：2018-06-01 00:00:00
abstract：:In order to evaluate the prevalence of gestational diabetes mellitus (GDM) and the presence of risk factors for GDM, we conducted a retrospective study of a cohort of Italian women. In addition, we compared universal versus selective screening to validate the ADA's recommendations in our population. From June 1st, 199...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2003.07.004
更新日期：2003-11-01 00:00:00
abstract：:Methylglyoxal and other alpha-oxoaldehydes are formed from glycolytic intermediates and may be involved in the development of diabetic microangiopathy. Glyoxalase I and glyoxalase II metabolise methylglyoxal to D-lactic acid, via the intermediate S-D-lactoylglutathione. The activities of the glyoxalase enzymes and the...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/0168-8227(89)90101-0
更新日期：1989-08-01 00:00:00
abstract：:In recent years, out-patient protocols have mainly displaced historical obstetric management of diabetic pregnancy. The impact of the change from centralized in-patient to decentralized out-patient treatment on glycaemic control and its effects on the outcome of newborns in diabetic pregnancies was therefore studied u...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/s0168-8227(99)00120-5
更新日期：2000-02-01 00:00:00
abstract：INTRODUCTION:Diabetes is a serious and increasing global health burden and estimates of prevalence are essential for appropriate allocation of resources and monitoring of trends. METHODS:We conducted a literature search of studies reporting the age-specific prevalence for diabetes and used the Analytic Hierarchy Proce...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2013.11.002
更新日期：2014-02-01 00:00:00
abstract：AIMS:To determine whether chronic life stress is differentially associated with quality of life (QoL) for Blacks vs. Hispanics with type 2 diabetes. METHODS:We assessed self-reported chronic stress and QoL in 125 patients with type 2 diabetes who self-identified as either non-Hispanic Black or Hispanic. Separate cross...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,多中心研究
doi：10.1016/j.diabres.2015.01.025
更新日期：2015-04-01 00:00:00
abstract：AIMS:The current study sought to identify patient-level factors related to health care use among White and minority men and women with diabetes. METHODS:A sample of 447 of non-pregnant individuals with diabetes, ages 18-64, was drawn from the 2015-2016 National Health and Nutrition Examination Surveys dataset. Poisson...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/j.diabres.2019.03.001
更新日期：2019-04-01 00:00:00
abstract：:Aminoguanidine, an inhibitor of advanced glycation reactions in vitro, inhibits the development of diabetic complications in animal models of diabetes, suggesting that it acts by inhibition of advanced glycation reactions in vivo. However, effects of aminoguanidine on the formation of specific advanced glycation end-p...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/s0168-8227(98)00121-1
更新日期：1999-02-01 00:00:00
abstract：:Mutations of mitochondrial DNA have been found in cardiomyopathic and diabetic patients as well as mitochondrial myopathic patients. Mitochondrial DNA mutations are maternally transmitted. It has also been reported that these mutations can be acquired under the influence of free radicals. This article is a mini-review...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,评审
doi：10.1016/0168-8227(96)01239-9
更新日期：1996-07-01 00:00:00
abstract：:In Japan, diabetic patients are known to be able to sit on the floor for a prolonged time because of mildness or absence of ischemic paresthesia. In order to investigate this resistance to ischemia quantitatively, the mixed nerve function of the median nerve was studied during 30 min of ischemia using surface electrod...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章
doi：10.1016/s0168-8227(86)80004-3
更新日期：1986-09-01 00:00:00
abstract：:To determine the status of diabetes care by general practitioners and diabetes specialists in Japan, we conducted a nation-wide cross-sectional survey. We asked 8112 clinics and hospitals randomly, from throughout Japan, to participate in this study and 721 facilities agreed. A total of 15,652 patients aged from 15 to...
journal_title：Diabetes research and clinical practice
pub_type： 杂志文章,多中心研究
doi：10.1016/j.diabres.2008.11.036
更新日期：2009-03-01 00:00:00
