Abstract:
:Micro- and nanomolar concentrations of ganglioside GM1 improved viability of neuronal PC12 cells under conditions of oxidative stress and reduced H2O2-induced ROS accumulation in these cells. These effects were more pronounced at micromolar concentrations. GM1 in concentrations of 100 nM and 10 μM significantly and substantially increased basal activity of protein kinase B (Akt) (the level of phosphorylated Akt form), but had virtually no effect on its expression in PC12 cells. In the presence of PI3K inhibitor LY294002 preventing protein kinase Akt activation, the protective effect of GM1 significantly decreased. These findings suggest that activation of protein kinase Akt by GM1 contributes to improvement of PC12 cell viability by this ganglioside.
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Zakharova IO,Sokolova TV,Akhmetshina AO,Avrova NFdoi
10.1007/s10517-015-3026-2subject
Has Abstractpub_date
2015-09-01 00:00:00pages
610-3issue
5eissn
0007-4888issn
1573-8221pii
10.1007/s10517-015-3026-2journal_volume
159pub_type
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