Role of Protein Kinase Akt Activation in Protective Effect of Ganglioside GM1 on PC12 Cells Exposed to H2O2.

Abstract:

:Micro- and nanomolar concentrations of ganglioside GM1 improved viability of neuronal PC12 cells under conditions of oxidative stress and reduced H2O2-induced ROS accumulation in these cells. These effects were more pronounced at micromolar concentrations. GM1 in concentrations of 100 nM and 10 μM significantly and substantially increased basal activity of protein kinase B (Akt) (the level of phosphorylated Akt form), but had virtually no effect on its expression in PC12 cells. In the presence of PI3K inhibitor LY294002 preventing protein kinase Akt activation, the protective effect of GM1 significantly decreased. These findings suggest that activation of protein kinase Akt by GM1 contributes to improvement of PC12 cell viability by this ganglioside.

journal_name

Bull Exp Biol Med

authors

Zakharova IO,Sokolova TV,Akhmetshina AO,Avrova NF

doi

10.1007/s10517-015-3026-2

subject

Has Abstract

pub_date

2015-09-01 00:00:00

pages

610-3

issue

5

eissn

0007-4888

issn

1573-8221

pii

10.1007/s10517-015-3026-2

journal_volume

159

pub_type

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