Abstract:
Objectives:Urinary tract infections are the second most common bacterial infections occurring at all ages and both sexes. The increasing rate of antibiotic resistance is a global concern. The use of routinely used antibiotics is resulting in treatment failure. The objective of this study was to diagnose the urinary tract infections by routine culture sensitivity test and by molecular methods. Methods:This study was conducted in Microbiology laboratory, Bolan Medical Complex Hospital, Quetta, from July 1st to 31st March 2019. Isolates were identified biochemically by API20E & API20NE. Antibiogram was performed using disc diffusion Kirby Bauer technique. The 16S rDNA gene approach was used for molecular identification of bacterial isolates. The presence of the blaNDM-1 gene was identified by polymerase chain reaction (PCR). Results:We isolated 146 bacterial isolates namely Escherichia coli (n=99) 67.80%, Klebsiellapneumoniae (n=33) 22.60%, Pseudomonas aeruginosa (n=11) 7.53% and Proteus mirabilis (n=3) 2.05% from 2032 urine samples. The resistance pattern was dominated by Multi Drug Resistance (MDR). Remarkably, four isolates of Escherichia coli (n=3) and Klebsiellapneumoniae (n=1) were displaying resistance against a range of antibiotics used in the study, including carbapenems but sensitive to tigecycline and polymyxins only, suggesting extensive drug resistance having bla NDM-1 gene. Conclusion:This is the first report on direct molecular detection of bacterial pathogens from urinary tract infected patients in Balochistan. The presence of blaNDM-1 in different bacterial species and their extensive drug resistance pattern poses a significant clinical threat. Molecular detection of bacteria and resistant gene may reduce the diagnostic time of patients.
journal_name
Pak J Med Scijournal_title
Pakistan journal of medical sciencesauthors
Pirkani GS,Awan MA,Abbas F,Din Mdoi
10.12669/pjms.36.3.1577subject
Has Abstractpub_date
2020-03-01 00:00:00pages
391-395issue
3eissn
1682-024Xissn
1681-715Xpii
PJMS-39-391journal_volume
36pub_type
杂志文章abstract:Objective:Hepatitis is a serious health concern with a high rate of mortality and morbidity world over. Saudi Arabia also has its course of the disease incidence. The data on the prevalence of the disease is still limiting. This study aimed to estimate the prevalence of hepatitis B virus [HBV] and hepatitis C virus [HC...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.342.14278
更新日期:2018-03-01 00:00:00
abstract:Objective:To find out the frequency of ZAP-70, CD38 and CD49d in patients diagnosed with CLL in our population. Methods:This is a cross sectional study conducted in Army Medical College in collaboration with Armed Forces Institute of Pathology and Military Hospital Rawalpindi from 1st January 2018 to 30th November 201...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.36.3.541
更新日期:2020-03-01 00:00:00
abstract:Objective:Zinc is a vital trace element and its deficiency in cirrhosis might potentiate the development of hepatic encephalopathy. The objective of this study was to assess the zinc levels in serum of patients having viral cirrhosis and compare it with normal healthy controls. Methods:This study was conducted in Depa...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.342.14457
更新日期:2018-03-01 00:00:00
abstract:Background & Objective:Routine physiotherapy has been advocated was an effective treatment for internal shoulder impingement syndrome. However, there is lack of best exercise treatment and lots of studies are under consideration. The objective of the study was to compare the effects of Neuromobilization and routine phy...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.36.4.1545
更新日期:2020-05-01 00:00:00
abstract:OBJECTIVE:To study the effect and clinical value of mammography in the diagnosis of breast lump so as to improve the diagnosis level of breast cancer. METHODS:A retrospective analysis was carried out on clinical data of 110 patients with mammary lump confirmed by pathology to study the compliance of mammography diagno...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.324.9384
更新日期:2016-07-01 00:00:00
abstract:OBJECTIVE:Emergency medicine staff is working at risk of blood-borne infections during their daily practice every time. The risk of transmission is higher when dealing with critically ill patients. Our objective was to find out the prevalence of Hepatitis B, Hepatitis C, and HIV, in critically ill red-coded emergency d...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.304.4975
更新日期:2014-07-01 00:00:00
abstract:OBJECTIVE:Ectopic pregnancy in the interstitial part of the Fallopian tubes can be life-threatining considering the thin myometrial tissue surrounding the gestational sac and highly vascularization which may result in catastrophic haemorrhage when interstitium is ruptured. The diagnosis and management is challenging. C...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.332.12093
更新日期:2017-03-01 00:00:00
abstract:Objective:To review the current status of departments of medical education in all public and private medical colleges located in the city of Lahore, Pakistan. Methods:This was a quantitative, cross sectional descriptive study; conducted from March to October 2015 in Pakistan Medical & Dental Council (PM&DC) recognized...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.346.15942
更新日期:2018-11-01 00:00:00
abstract:OBJECTIVE:To explore the factors associated with preoperative epileptic seizure and surgical outcome in patients with cerebral gangliolioma (GG). METHODS:A total of 31 consecutive patients with pathologically confirmed ganglioglioma and surgically treated from January 2003 to June 2011 in West China Hospital of Sichua...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.302.4362
更新日期:2014-03-01 00:00:00
abstract:OBJECTIVE:Ventilator Associated Pneumonia (VAP) has an imperative place amongst nosocomial infections leading to increase morbidity and mortality rates. The present study aimed to determine risk factors for acquisition of ventilator- associated pneumonia in an intensive care unit (ICU). METHODS:A nested case-control s...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.295.3375
更新日期:2013-09-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to investigate the difference of liver function changes according to the liver regeneration rate after liver transplantation through blood tests. METHODS:Fifty donors, who underwent computed tomography (CT) 3D volumetry, were analyzed before and after liver transplantation. CT 3D v...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.311.5864
更新日期:2015-01-01 00:00:00
abstract:Background and Objectives:Learning is an interplay between cognition and environmental factors. Any learning environment, that fulfills the intrinsic and extrinsic needs of the students will probably lead to better and more promising learning outcomes. This study aimed to investigate the student perceptions of Learning...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.35.3.712
更新日期:2019-01-01 00:00:00
abstract:OBJECTIVE:The objective was to determine the job satisfaction and burnout levels of primary care health workers in Malatya in Turkey. METHODS:The sample of the study included 186 physicians, 126 midwives and 106 nurses working in primary health care. The Minnesota Job Satisfaction Scale and the Maslach Burnout Scale w...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.313.6795
更新日期:2015-01-01 00:00:00
abstract::Trans fatty acids (TFA) are produced either by hydrogenation of unsaturated oils or by biohydrogenation in the stomach of ruminant animals. Vanaspati ghee and margarine have high contents of TFA. A number of studies have shown an association of TFA consumption and increased risk of cardiovascular disease (CVD). This i...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章,评审
doi:10.12669/pjms.301.4525
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:To determine the frequency and characteristics of dengue fever (DF) in patients of acute febrile illness presenting at a secondary care hospital. METHODOLOGY:The observational cross sectional study was carried out from May to October 2010 in Remedial Centre Karachi and included patients above the age of 12 y...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.291.2742
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:Fentanyl is frequently used during anesthesia induction. The use of fentanyl can cause cough through different mechanisms. Here, we aimed to investigate effects of pheniramine maleate (PM), an antihistaminic agent, and compare it with lidocaine on fentanyl induced cough. METHODS:This is a randomized double-b...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.323.9496
更新日期:2016-05-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Breakpoint cluster region-Abelson (BCR-ABL) rearrangement or Philadelphia (Ph) chromosome in Chronic Myeloid Leukemia (CML) is derived from a reciprocal chromosomal translocation between ABL gene on chromosome 9 and BCR gene on chromosome 22. This chimeric protein has various sizes and therefor...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:
更新日期:2014-07-01 00:00:00
abstract:UNLABELLED:Objectives : The aim of this study was to determine the frequency of otomycosis, the clinical presentation, predisposing factors and treatment outcomes. METHODS:This observational study was conducted at ENT department of Combined Military Hospital Attock, from October, 2010 to September, 2012. Convenient sa...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.303.4106
更新日期:2014-05-01 00:00:00
abstract:Objective:To determine the cases of Noninvasive Follicular thyroid neoplasm with papillary like nuclear features (NIFTP) in Pakistani population retrospectively. Another objective was to determine their clinical and radiological outcomes with respect to local and systemic disease recurrence, reconfirming the benign cou...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.36.2.1123
更新日期:2020-01-01 00:00:00
abstract:Objective:To study the clinical, biochemical and radiological features and management outcomes of patients with primary hyperparathyroidism. Methods:This retrospective study was conducted at the Aga Khan University Hospital, Karachi, Pakistan and comprised data of patients with primary hyperparathyroidism from 2008 to...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.36.6.2572
更新日期:2020-09-01 00:00:00
abstract:Background and Objective:Brucellosis is an important zoonotic disease and a major public health problem in the Middle East countries including Saudi Arabia. This study has evaluated the seroprevalence of human brucellosis in Wadi Al Dawaser region of Central Saudi Arabia. Methods:The study was conducted for three year...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.35.1.55
更新日期:2019-01-01 00:00:00
abstract:OBJECTIVE:To explore the relationship between tea drinking habits and osteoporotic hip/femur fractures. METHODS:Paired case-control method was used for face-to-face interviews from January 2010 to June 2014. Patients (n=435) with newly osteoporotic hip/femur fracture and 435 controls with the same gender and age (±3) ...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.322.9092
更新日期:2016-03-01 00:00:00
abstract:Objective:To find the perceived stress level and monetary burden in the thalassemia patients and their caregivers related to thalassemia treatment sessions. Methods:A descriptive cross sectional study was conducted at Rawalpindi Thalassemia Centre and Military Hospital Rawalpindi of six months' duration from November ...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.344.15420
更新日期:2018-07-01 00:00:00
abstract:Background & Objective:Catalase (CAT) is an important endogenous antioxidant enzyme that detoxifies H2O2 into water and oxygen, consequently limiting the deleterious effects of reactive oxygen species. It has suggested that CAT-21A/T (rs7943316) OMIM: 115500 gene promoter polymorphism is predominantly associated with d...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.336.13188
更新日期:2017-11-01 00:00:00
abstract:OBJECTIVE:To investigate the role of Brachial ankle Pulse Wave Relocity (baPWV) and cfPWV on the risk of Coronary artery disease and the interaction between baPWV and risk factors of Coronary artery disease (CAD). METHODS:A case-control study was conducted at Department of Emergency, SunYat-Sen memorial Hospital, Chin...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.306.5584
更新日期:2014-11-01 00:00:00
abstract:Background and Objective:Cardiovascular deaths usually occur in older pregnancies and arrhythmia is the third most common cause. Our study aimed to determine whether the risk of arrhythmia increases in pregnancy with advanced age. Methods:In total, 280 pregnant women, of whom 98 were of advanced age and 182 were under...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.343.14313
更新日期:2018-05-01 00:00:00
abstract:Objective:To develop a tool to evaluate faculty perceptions of assessment quality in an undergraduate medical program. Methods:The Assessment Implementation Measure (AIM) tool was developed by a mixed method approach. A preliminary questionnaire developed through literature review was submitted to a panel of 10 medica...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.341.14354
更新日期:2018-01-01 00:00:00
abstract:Objective:The aim of the study was to assess the failure loads and compressive stresses among bilayered press on Y-TZP (POZ) and press on metal (POM) crowns with different core-veneer thickness. Methods:Thirty metal and Y-TZP copings were fabricated using CAD-CAM technology with specified thickness. All copings were v...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.36.7.2472
更新日期:2020-11-01 00:00:00
abstract:Objective:The study examines the level and source of perceived social support in pregnant adolescents and the factors related to their perception of social support. Methods:This descriptive study was conducted with the voluntary participation of 127 adolescent pregnant females who visited the Gynecology and Pediatric ...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.341.14221
更新日期:2018-01-01 00:00:00
abstract:OBJECTIVE:To determine the various spectrum of pancytopenia with its frequency on the basis of bone marrow examination in children from 6 months to 14 years. METHODS:A retrospective descriptive study was carried out at Department of Pediatric Rehman Medical Institute Peshawar from January 2006 to December 2012. A tota...
journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.295.3865
更新日期:2013-09-01 00:00:00