Abstract:
:The potential use of 13C multilabeled substrates has been studied in biological applications using in vivo and in vitro proton and 13C NMR spectroscopy. In 13C NMR spectroscopy, multilabeled compounds allow the simultaneous observation of several nuclei or increase distinctly the signal to noise ratio due to a higher degree of enrichment. Contiguous labeling of substrates leads to homonuclear 13C-13C spin couplings and provides a simple means to distinguish between endogenous stores of metabolites and metabolites derived from added substrates.
journal_name
NMR Biomedjournal_title
NMR in biomedicineauthors
Künnecke B,Cerdan Sdoi
10.1002/nbm.1940020516subject
Has Abstractpub_date
1989-12-01 00:00:00pages
274-7issue
5-6eissn
0952-3480issn
1099-1492journal_volume
2pub_type
杂志文章abstract::One of the hallmarks of diffusion NMR and MRI is its ability to utilize restricted diffusion to probe compartments much smaller than the excited volume or the MRI voxel, respectively, and to extract microstructural information from them. Single-pulsed field gradient (s-PFG) MR methodologies have been employed with gre...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.1550
更新日期:2010-08-01 00:00:00
abstract::Diffusion tensor MRI (DTI) is a method for the noninvasive assessment of cellular organization and integrity in vivo. In this study, in vivo DTI was performed to demonstrate its ability to reflect photoreceptor cell alignment in adult C57BL/6 wild-type mice. Age-matched retinal degeneration 1 (rd1) mice were employed ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1609
更新日期:2011-06-01 00:00:00
abstract::Recent work has shown that solid-state (1) H and (31) P MRI can provide detailed insight into bone matrix and mineral properties, thereby potentially enabling differentiation of osteoporosis from osteomalacia. However, (31) P MRI of bone mineral is hampered by unfavorable relaxation properties. Hence, accurate knowled...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.2930
更新日期:2013-09-01 00:00:00
abstract::Development and evaluation of new anticancer drugs are expedited when minimally invasive biomarkers of pharmacokinetic and pharmacodynamic behaviour are available. Gene-directed enzyme prodrug therapy (GDEPT) is a suicide gene therapy in which the anticancer drug is activated in the tumor by an exogenous enzyme previo...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1375
更新日期:2009-06-01 00:00:00
abstract::We describe the optimal high-level postprocessing of single-voxel (1)H magnetic resonance spectra and assess the benefits and limitations of automated methods as diagnostic aids in the detection of recurrent brain tumor. In a previous clinical study, 90 long-echo-time single-voxel spectra were obtained from 52 patient...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1041
更新日期:2006-08-01 00:00:00
abstract::Perfusion is an important biomarker of tissue function and has been associated with tumor pathophysiology such as angiogenesis and hypoxia. Arterial spin labeling (ASL) MRI allows noninvasive and quantitative imaging of perfusion; however, the application in mouse xenograft tumor models has been challenging due to the...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3330
更新日期:2015-08-01 00:00:00
abstract::Lung cancer is a primary cause of cancer deaths worldwide. Timely detection of this pathology is necessary to delay or interrupt lung cancer progression, ultimately resulting in a possible better prognosis for the patient. In this context, magnetic resonance imaging (MRI) is especially promising. Ultra-short echo time...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3790
更新日期:2017-11-01 00:00:00
abstract::Owing to the small quantity of tissue available in human biopsy specimens, aqueous and lipid components often have to be determined in the same tissue sample. Perchloric acid (PCA) used for the extraction of aqueous metabolites has a deleterious effect on lipid components; the severity of the damage is not known. In t...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1159
更新日期:2008-02-01 00:00:00
abstract::MR Z-spectroscopy allows enhanced imaging contrast on the basis of saturation transfer between the proton pools of cellular compounds and water, occurring via chemical exchange (chemical exchange saturation transfer, CEST) or dipole-dipole coupling (nuclear Overhauser effect, NOE). In previous studies, signals observe...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3021
更新日期:2013-12-01 00:00:00
abstract::High-energy phosphate metabolism, which allows the synthesis and regeneration of adenosine triphosphate (ATP), is a vital process for neuronal survival and activity. In particular, creatine kinase (CK) serves as an energy reservoir for the rapid buffering of ATP levels. Altered CK enzyme activity, reflecting compromis...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1636
更新日期:2011-08-01 00:00:00
abstract::The development and implementation of novel MRI pulse sequences remains challenging and laborious. Gradient waveforms are typically designed using a combination of analytical and ad hoc methods to construct each gradient waveform axis independently. This strategy makes coding the pulse sequence complicated, in additio...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.4308
更新日期:2020-12-01 00:00:00
abstract::Despite 40 years of NMR spectroscopy and 10 years of in vivo spectroscopy in humans, the break-even point for routine clinical diagnosis has not yet been reached. Obviously there are many factors that stimulate but also restrict the use of proton NMR spectroscopy in clinical routine. ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940070803
更新日期:1994-12-01 00:00:00
abstract::Resolution enhancement for glutamate (Glu), glutamine (Gln) and glutathione (GSH) in the human brain by TE-optimized point-resolved spectroscopy (PRESS) at 7 T is reported. Sub-TE dependences of the multiplets of Glu, Gln, GSH, γ-aminobutyric acid (GABA) and N-acetylaspartate (NAA) at 2.2-2.6 ppm were investigated wit...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1529
更新日期:2010-11-01 00:00:00
abstract::Normal subjects performed voluntary, isometric exercise 1 s contraction, 1 s rest for 10 min) of the first dorsal interosseous (FDI) muscle with a target force of 25, 50 and 100% of the maximal voluntary contraction (MVC) force. 31P NMR spectra were collected continuously before, during and after exercise. Data were a...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940030504
更新日期:1990-10-01 00:00:00
abstract::Glycine (Gly) has been implicated in several neurological disorders, including malignant brain tumors. The precise measurement of Gly is challenging largely as a result of the spectral overlap with myo-inositol (mI). We report a new triple-refocusing sequence for the reliable co-detection of Gly and mI at 3 T and for ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3747
更新日期:2017-09-01 00:00:00
abstract::The dependence of apparent diffusion coefficients (ADCs) of molecules in biological tissues on an acquisition-specific timescale is a powerful mechanism for studying tissue microstructure. Unlike water, metabolites are confined mainly to intracellular compartments, thus providing higher specificity to tissue microstru...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3085
更新日期:2014-05-01 00:00:00
abstract::Transmit efficiency specifies the amplitude of the magnetic resonance excitation field produced over a region of interest with respect to the radiofrequency (RF) power deposited in the sample. This metric is highly important at ultra-high field magnetic resonance imaging (≥7 T), where excitation inhomogeneities and el...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.4383
更新日期:2020-11-01 00:00:00
abstract::Results of the evaluation of transrectal ultrasound (TRUS) guided needle biopsy of voxels identified as suspicious of malignancy on magnetic resonance spectroscopic imaging (MRSI) in a large cohort of men (n = 83) with abnormal digital rectal examination (DRE) [prostate specific antigen (PSA) 0-4 ng/ml] or PSA less th...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1083
更新日期:2007-02-01 00:00:00
abstract::One of the most commonly used methods for in vivo MRS detection of γ-aminobutyric acid (GABA) is the MEGA-point-resolved spectroscopy (MEGA-PRESS) technique. However, accurate quantification of GABA using MEGA-PRESS is complicated by spectral co-editing of macromolecular resonances. In this article, a new pulse sequen...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1688
更新日期:2011-12-01 00:00:00
abstract::Qualitative and quantitative (1) H NMR analysis of lipid extracts of gallbladder tissue in chronic cholecystitis (CC, benign) (n = 14), xanthogranulomatous cholecystitis (XGC, intermediate) (n = 9) and gallbladder cancer (GBC, malignant) (n = 8) was carried out to understand the mechanisms involved in the transformati...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1594
更新日期:2011-05-01 00:00:00
abstract::The signal decay with increasing b-factor at fixed echo time from brain tissue in vivo has been measured using a line scan Stejskal-Tanner spin echo diffusion approach in eight healthy adult volunteers. The use of a 175 ms echo time and maximum gradient strengths of 10 mT/m allowed 64 b-factors to be sampled, ranging ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/(sici)1099-1492(199902)12:1<51::aid-nbm546
更新日期:1999-02-01 00:00:00
abstract::The measurement of pulmonary perfusion (blood delivered to the capillary bed within a voxel) using arterial spin labeling (ASL) magnetic resonance imaging is often complicated by signal artifacts from conduit vessels that carry blood destined for voxels at a distant location in the lung. One approach to dealing with c...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3358
更新日期:2015-09-01 00:00:00
abstract::Citicoline supplementation has been used to ameliorate memory disturbances in older people and those with Alzheimer's disease. This study used MRS to characterize the effects of citicoline on high-energy phosphate metabolites and constituents of membrane synthesis in the frontal lobe. Phosphorus ((31)P) metabolite dat...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1281
更新日期:2008-11-01 00:00:00
abstract::Localization techniques are considered an indispensable part of the clinical spectroscopy examination. The results are often a marked improvement in diagnostic information (amplification), but because of the introduction of new artifacts and the more prolonged examination time, some of the undoubted advantage is lost ...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.1940020525
更新日期:1989-12-01 00:00:00
abstract::The phosphorus NMR spectra of isolated perfused rat liver displays a prominent inorganic phosphate peak at 2.76 +/- 0.05 ppm relative to liver glycerolphosphocholine at 0.49 ppm. From titration curves of phosphorus-containing compounds this corresponds to a pH of 7.4. The spectra also display a shoulder on the promine...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940010207
更新日期:1988-04-01 00:00:00
abstract::The purpose of this study was to identify the optimal tracer kinetic model from T1 -weighted dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) data and evaluate whether parameters estimated from the optimal model predict tumor aggressiveness determined from histopathology in patients with papillary thyroi...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.4166
更新日期:2020-01-01 00:00:00
abstract::This study investigated the association between the total choline (tCho) concentration and the clinical characteristics and biomarker status of breast cancer. Sixty-two patients with breast cancer, 1.5 cm or larger in size on MR images, were studied. The tCho concentration was correlated with the MRI features, contra...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1595
更新日期:2011-04-01 00:00:00
abstract::The detection of Parkinson's disease (PD) in its preclinical stages prior to outright neurodegeneration is essential to the development of neuroprotective therapies and could reduce the number of misdiagnosed patients. However, early diagnosis is currently hampered by lack of reliable biomarkers. (1) H magnetic resona...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.2817
更新日期:2013-01-01 00:00:00
abstract::The aim of the study was to establish the normal range and to evaluate the reproducibility of dynamic contrast enhanced MRI (DCE-MRI) parameter estimates in normal human pelvic tissues. Nineteen patients with prostate cancer, undergoing androgen deprivation treatment, had paired DCE-MRI examinations of the pelvis usin...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.732
更新日期:2002-04-01 00:00:00
abstract::Spin-exchange NMR techniques enable the measurement of the rates of exchange of solutes between chemically or physically distinct sites in reactions taking place at chemical equilibrium. The time scale of the events that are able to be investigated lies in the neighbourhood of 1 s. The earliest studies in this area of...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.1940030303
更新日期:1990-06-01 00:00:00