Insight Into Function and Subcellular Localization of Plasmopara viticola Putative RxLR Effectors.

Abstract:

:Grapevine downy mildew, caused by oomycete fungus Plasmopara viticola, is one of the most devastating diseases of grapes across the major production regions of the world. Although many putative effector molecules have been identified from this pathogen, the functions of the majority of these are still unknown. In this study, we analyzed the potential function of 26 P. viticola effectors from the highly virulent strain YL. Using transient expression in leaf cells of the tobacco Nicotiana benthamiana, we found that the majority of the effectors could suppress cell death triggered by BAX and INF1, while seven could induce cell death. The subcellular localization of effectors in N. benthamiana was consistent with their localization in cells of Vitis vinifera. Those effectors that localized to the nucleus (17/26) showed a variety of subnuclear localization. Ten of the effectors localized predominantly to the nucleolus, whereas the remaining seven localized to nucleoplasm. Interestingly, five of the effectors were strongly related in sequence and showed identical subcellular localization, but had different functions in N. benthamiana leaves and expression patterns in grapevine in response to P. viticola. This study highlights the potential functional diversity of P. viticola effectors.

journal_name

Front Microbiol

authors

Chen T,Liu R,Dou M,Li M,Li M,Yin X,Liu GT,Wang Y,Xu Y

doi

10.3389/fmicb.2020.00692

subject

Has Abstract

pub_date

2020-04-21 00:00:00

pages

692

issn

1664-302X

journal_volume

11

pub_type

杂志文章
  • Identification of the Replication Origins from Cyanothece ATCC 51142 and Their Interactions with the DnaA Protein: From In Silico to In Vitro Studies.

    abstract::Based on the complete genome of Cyanothece ATCC 51142, the oriCs of both the circular and linear chromosomes in Cyanothece ATCC 51142 have been predicted by utilizing a web-based system Ori-Finder. Here, we provide experimental support for the results of Ori-Finder to identify the replication origins of Cyanothece ATC...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01370

    authors: Huang H,Song CC,Yang ZL,Dong Y,Hu YZ,Gao F

    更新日期:2015-12-10 00:00:00

  • Comparison among the Quantification of Bacterial Pathogens by qPCR, dPCR, and Cultural Methods.

    abstract::The demand for rapid methods for the quantification of pathogens is increasing. Among these methods, those based on nucleic acids amplification (quantitative PCRs) are the most widespread worldwide. Together with the qPCR, a new approach named digital PCR (dPCR), has rapidly gained importance. The aim of our study was...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01174

    authors: Ricchi M,Bertasio C,Boniotti MB,Vicari N,Russo S,Tilola M,Bellotti MA,Bertasi B

    更新日期:2017-06-28 00:00:00

  • Genome Analysis of Clostridium difficile PCR Ribotype 014 Lineage in Australian Pigs and Humans Reveals a Diverse Genetic Repertoire and Signatures of Long-Range Interspecies Transmission.

    abstract::Clostridium difficile PCR ribotype (RT) 014 is well-established in both human and porcine populations in Australia, raising the possibility that C. difficile infection (CDI) may have a zoonotic or foodborne etiology. Here, whole genome sequencing and high-resolution core genome phylogenetics were performed on a contem...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.02138

    authors: Knight DR,Squire MM,Collins DA,Riley TV

    更新日期:2017-01-11 00:00:00

  • Saccharomyces cerevisiae - Insects Association: Impacts, Biogeography, and Extent.

    abstract::Over the last few years, an increasing number of studies have reported the existence of an association between the budding yeast Saccharomyces cerevisiae and insects. The discovery of this relationship has called into question the hypothesis that S. cerevisiae is unable to survive in nature and that the presence of S....

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.01629

    authors: Meriggi N,Di Paola M,Cavalieri D,Stefanini I

    更新日期:2020-07-14 00:00:00

  • The N-Terminus of Human Lactoferrin Displays Anti-biofilm Activity on Candida parapsilosis in Lumen Catheters.

    abstract::Candida parapsilosis is a major cause of hospital-acquired infection, often related to parenteral nutrition administered via catheters and hand colonization of health care workers, and its peculiar biofilm formation ability on plastic surfaces. The mortality rate of 30% points to the pressing need for new antifungal d...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02218

    authors: Fais R,Di Luca M,Rizzato C,Morici P,Bottai D,Tavanti A,Lupetti A

    更新日期:2017-11-13 00:00:00

  • Different metabolic features of Bacteroides fragilis growing in the presence of glucose and exopolysaccharides of bifidobacteria.

    abstract::Bacteroides is among the most abundant microorganism inhabiting the human intestine. They are saccharolytic bacteria able to use dietary or host-derived glycans as energy sources. Some Bacteroides fragilis strains contribute to the maturation of the immune system but it is also an opportunistic pathogen. The intestine...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00825

    authors: Rios-Covian D,Sánchez B,Salazar N,Martínez N,Redruello B,Gueimonde M,de Los Reyes-Gavilán CG

    更新日期:2015-08-18 00:00:00

  • Microbial resource utilization traits and trade-offs: implications for community structure, functioning, and biogeochemical impacts at present and in the future.

    abstract::Trait-based approaches provide a mechanistic framework to understand and predict the structure and functioning of microbial communities. Resource utilization traits and trade-offs are among key microbial traits that describe population dynamics and competition among microbes. Several important trade-offs have been ide...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00254

    authors: Litchman E,Edwards KF,Klausmeier CA

    更新日期:2015-04-08 00:00:00

  • Algae-based oral recombinant vaccines.

    abstract::Recombinant subunit vaccines are some of the safest and most effective vaccines available, but their high cost and the requirement of advanced medical infrastructure for administration make them impractical for many developing world diseases. Plant-based vaccines have shifted that paradigm by paving the way for recomb...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2014.00060

    authors: Specht EA,Mayfield SP

    更新日期:2014-02-17 00:00:00

  • Antimicrobial and Antibiofilm Potential of Acyclic Amines and Diamines against Multi-Drug Resistant Staphylococcus aureus.

    abstract::Multi-drug resistant Staphylococcus aureus (MDRSA) remains a great challenge despite a decade of research on antimicrobial compounds against their infections. In the present study, various acyclic amines and diamines were chemically synthesized and tested for their antimicrobial as well as antibiofilm activity against...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01767

    authors: Kaur G,Balamurugan P,Vasudevan S,Jadav S,Princy SA

    更新日期:2017-09-15 00:00:00

  • Relevance of GC content to the conservation of DNA polymerase III/mismatch repair system in Gram-positive bacteria.

    abstract::The mechanism of DNA replication is one of the driving forces of genome evolution. Bacterial DNA polymerase III, the primary complex of DNA replication, consists of PolC and DnaE. PolC is conserved in Gram-positive bacteria, especially in the Firmicutes with low GC content, whereas DnaE is widely conserved in most Gra...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2013.00266

    authors: Akashi M,Yoshikawa H

    更新日期:2013-09-17 00:00:00

  • PMQR genes oqxAB and aac(6')Ib-cr accelerate the development of fluoroquinolone resistance in Salmonella typhimurium.

    abstract::Emergence of multidrug-resistant Salmonella typhimurium strains, especially the ACSSuT and nalidixic acid R types, has significantly compromised the effectiveness of current strategies to control Salmonella infections, resulting in increased morbidity and mortality. Clinical S. typhimurium isolates recovered in Hong K...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00521

    authors: Wong MH,Chan EW,Liu LZ,Chen S

    更新日期:2014-10-02 00:00:00

  • Population Genomics of an Obligately Halophilic Basidiomycete Wallemia ichthyophaga.

    abstract:Background:Wallemia ichthyophaga is a highly specialized basidiomycetous fungus. It is one of the most halophilic fungi ever described, only able to grow at low water activity. This specialization is thought to explain why it is only rarely isolated from nature. Results:Genomes of 21 W. ichthyophaga strains were seque...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02019

    authors: Gostinčar C,Sun X,Zajc J,Fang C,Hou Y,Luo Y,Gunde-Cimerman N,Song Z

    更新日期:2019-09-04 00:00:00

  • Probiotic Enterococcus mundtii Isolate Protects the Model Insect Tribolium castaneum against Bacillus thuringiensis.

    abstract::Enterococcus mundtii strains isolated from the larval feces of the Mediterranean flour moth Ephestia kuehniella show antimicrobial activity against a broad spectrum of Gram-positive and Gram-negative bacteria. The in vitro probiotic characterization of one isolate revealed a high auto-aggregation score, a hydrophilic ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01261

    authors: Grau T,Vilcinskas A,Joop G

    更新日期:2017-07-07 00:00:00

  • Probiotic Supplements: Hope or Hype?

    abstract::Probiotic bacteria have been associated with various health benefits and included in overwhelming number of foods. Today, probiotic supplements are consumed with increasing regularity and record a rapidly growing economic value. With billions of heterogeneous populations of probiotics per serving, probiotic supplement...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00160

    authors: Wang Y,Jiang Y,Deng Y,Yi C,Wang Y,Ding M,Liu J,Jin X,Shen L,He Y,Wu X,Chen X,Sun C,Zheng M,Zhang R,Ye H,An H,Wong A

    更新日期:2020-02-28 00:00:00

  • Fungal Diversity and Mycotoxins in Low Moisture Content Ready-To-Eat Foods in Nigeria.

    abstract::Low moisture content ready-to-eat foods vended in Nigerian markets could be pre-packaged or packaged at point of sale. These foods are widely and frequently consumed across Nigeria as quick foods. Despite their importance in the daily diets of Nigerians, a comprehensive study on the diversity of fungi, fungal metaboli...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00615

    authors: Ezekiel CN,Oyedele OA,Kraak B,Ayeni KI,Sulyok M,Houbraken J,Krska R

    更新日期:2020-04-09 00:00:00

  • Upregulation of the PatAB Transporter Confers Fluoroquinolone Resistance to Streptococcus pseudopneumoniae.

    abstract::We characterized the mechanism of fluoroquinolone-resistance in two isolates of Streptococcus pseudopneumoniae having fluoroquinolone-efflux as unique mechanism of resistance. Whole genome sequencing and genetic transformation experiments were performed together with phenotypic determinations of the efflux mechanism. ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02074

    authors: Alvarado M,Martín-Galiano AJ,Ferrándiz MJ,Zaballos Á,de la Campa AG

    更新日期:2017-10-26 00:00:00

  • Antiviral Activity of Chicken Cathelicidin B1 Against Influenza A Virus.

    abstract::Cathelicidins (CATHs) are host defense peptides (HDPs) that play an important role in the innate immune response against infections. Although multiple functions of cathelicidins have been described, including direct antimicrobial activity and several immunomodulatory effects on the host, relatively little is known abo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00426

    authors: Peng L,Du W,Balhuizen MD,Haagsman HP,de Haan CAM,Veldhuizen EJA

    更新日期:2020-03-19 00:00:00

  • Gymnemic Acids Inhibit Adhesive Nanofibrillar Mediated Streptococcus gordonii-Candida albicans Mono-Species and Dual-Species Biofilms.

    abstract::Dental caries and periodontitis are the most common oral disease of all age groups, affecting billions of people worldwide. These oral diseases are mostly associated with microbial biofilms in the oral cavity. Streptococcus gordonii, an early tooth colonizing bacterium and Candida albicans, an opportunistic pathogenic...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02328

    authors: Veerapandian R,Vediyappan G

    更新日期:2019-10-11 00:00:00

  • Potential drivers of microbial community structure and function in Arctic spring snow.

    abstract::The Arctic seasonal snowpack can extend at times over a third of the Earth's land surface. This chemically dynamic environment interacts constantly with different environmental compartments such as atmosphere, soil and meltwater, and thus, strongly influences the entire biosphere. However, the microbial community asso...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00413

    authors: Maccario L,Vogel TM,Larose C

    更新日期:2014-08-07 00:00:00

  • Seasonal dynamics of bacterial and archaeal methanogenic communities in flooded rice fields and effect of drainage.

    abstract::We studied the resident (16S rDNA) and the active (16S rRNA) members of soil archaeal and bacterial communities during rice plant development by sampling three growth stages (vegetative, reproductive and maturity) under field conditions. Additionally, the microbial community was investigated in two non-flooded fields ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00752

    authors: Breidenbach B,Conrad R

    更新日期:2015-01-08 00:00:00

  • The Microbiome of Prostate Fluid Is Associated With Prostate Cancer.

    abstract:Objectives:To explore the microbiome of the prostatic fluid in high prostate-specific antigen (PSA) patients. Patients and Methods:The microbiome profiles of prostatic fluid samples from 32 prostate cancer (PCa) patients and 27 non-PCa people were assessed. Microbiome analysis was assessed by massive 16S ribosomal RNA...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01664

    authors: Ma X,Chi C,Fan L,Dong B,Shao X,Xie S,Li M,Xue W

    更新日期:2019-07-19 00:00:00

  • The Diverse Functional Roles of Elongation Factor Tu (EF-Tu) in Microbial Pathogenesis.

    abstract::Elongation factor thermal unstable Tu (EF-Tu) is a G protein that catalyzes the binding of aminoacyl-tRNA to the A-site of the ribosome inside living cells. Structural and biochemical studies have described the complex interactions needed to effect canonical function. However, EF-Tu has evolved the capacity to execute...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2019.02351

    authors: Harvey KL,Jarocki VM,Charles IG,Djordjevic SP

    更新日期:2019-10-24 00:00:00

  • Oral Supplementation of Lead-Intolerant Intestinal Microbes Protects Against Lead (Pb) Toxicity in Mice.

    abstract::Oral exposure to the heavy metal lead (Pb) causes various dysfunctions in animals. However, the influence of gut bacteria on Pb absorption, bioaccumulation, and excretion is largely unknown. In this study, we use a mouse model to investigate the relationship between gut microbiota, Pb-intolerant intestinal microbes an...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.03161

    authors: Zhai Q,Qu D,Feng S,Yu Y,Yu L,Tian F,Zhao J,Zhang H,Chen W

    更新日期:2020-01-22 00:00:00

  • A Single Amino Acid Substitution in the Matrix Protein (M51R) of Vesicular Stomatitis New Jersey Virus Impairs Replication in Cultured Porcine Macrophages and Results in Significant Attenuation in Pigs.

    abstract::In this study, we explore the virulence of vesicular stomatitis New Jersey virus (VSNJV) in pigs and its potential relationship with the virus's ability to modulate innate responses. For this purpose, we developed a mutant of the highly virulent strain NJ0612NME6, containing a single amino acid substitution in the mat...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01123

    authors: Velazquez-Salinas L,Pauszek SJ,Holinka LG,Gladue DP,Rekant SI,Bishop EA,Stenfeldt C,Verdugo-Rodriguez A,Borca MV,Arzt J,Rodriguez LL

    更新日期:2020-05-29 00:00:00

  • Origin, diversity, and maturation of human antiviral antibodies analyzed by high-throughput sequencing.

    abstract::Our understanding of how antibodies are generated and function could help develop effective vaccines and antibody-based therapeutics against viruses such as HIV-1, SARS coronavirus (SARS CoV), and Hendra and Nipah viruses (henipaviruses). Although broadly neutralizing antibodies (bnAbs) against the HIV-1 were observed...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00277

    authors: Prabakaran P,Zhu Z,Chen W,Gong R,Feng Y,Streaker E,Dimitrov DS

    更新日期:2012-08-02 00:00:00

  • Separating the Empirical Wheat From the Pseudoscientific Chaff: A Critical Review of the Literature Surrounding Glyphosate, Dysbiosis and Wheat-Sensitivity.

    abstract::The prevalence of digestive disorders has increased globally, as countries have adopted a more "Westernized" diet pattern. A Western diet, characterized as high in fat and refined carbohydrates, can also be defined as a product of increased technology and industrialization. Modern farmers rely on agrochemicals to meet...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.556729

    authors: Barnett JA,Gibson DL

    更新日期:2020-09-25 00:00:00

  • Combatting cyanobacteria with hydrogen peroxide: a laboratory study on the consequences for phytoplankton community and diversity.

    abstract::Experiments with different phytoplankton densities in lake samples showed that a high biomass increases the rate of hydrogen peroxide (HP) degradation and decreases the effectiveness of HP in the selective suppression of dominant cyanobacteria. However, selective application of HP requires usage of low doses only, acc...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00714

    authors: Weenink EF,Luimstra VM,Schuurmans JM,Van Herk MJ,Visser PM,Matthijs HC

    更新日期:2015-07-22 00:00:00

  • Metabolic Response of "Candidatus Accumulibacter Phosphatis" Clade II C to Changes in Influent P/C Ratio.

    abstract::The objective of this study was to investigate the ability of a culture highly enriched with the polyphosphate-accumulating organism, "Candidatus Accumulibacter phosphatis" clade IIC, to adjust their metabolism to different phosphate availabilities. For this purpose the biomass was cultivated in a sequencing batch rea...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.02121

    authors: Welles L,Abbas B,Sorokin DY,Lopez-Vazquez CM,Hooijmans CM,van Loosdrecht MC,Brdjanovic D

    更新日期:2017-01-05 00:00:00

  • Applications of Wild Isolates of Saccharomyces Yeast for Industrial Fermentation: The Gut of Social Insects as Niche for Yeast Hybrids' Production.

    abstract::In the industry of fermented food and beverages, yeast cultures are often selected and standardized in order to ensure a better control of fermentation and a more stable product over time. Several studies have shown that the organoleptic characteristics of fermented products reflect geographic variations of the microb...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.578425

    authors: Di Paola M,Meriggi N,Cavalieri D

    更新日期:2020-10-29 00:00:00

  • Effects of Sulfamethoxazole on the Microbial Community Dynamics During the Anaerobic Digestion Process.

    abstract::Anaerobic digestion (AD) treatment of cattle manure and slurry makes it possible to produce biogas, a renewable and storable biofuel, as well as digestate, a residual organic matter that can be used to replace chemical fertilizers. On the other hand, the intense use of antibiotics (e.g., sulfamethoxazole) in animal hu...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.537783

    authors: Mazzurco Miritana V,Massini G,Visca A,Grenni P,Patrolecco L,Spataro F,Rauseo J,Garbini GL,Signorini A,Rosa S,Barra Caracciolo A

    更新日期:2020-09-16 00:00:00