Abstract:
:Multiepitope peptide vaccine has some advantages over traditional recombinant protein vaccine due to its easy and fast production and possible inclusion of multiple protective epitopes of pathogens. However, it is usually poorly immunogenic and needs to conjugate to a large carrier protein. Peptides conjugated to a central lysine core to form multiple antigen peptides (MAPs) will increase the immunogenicity of peptide vaccine. In this study, we constructed a MAP consisting of CD4+ T cell and B cell epitopes of paramyosin (Pmy) of Trichinella spiralis (Ts-Pmy), which has been proved to be a good vaccine candidate in our previous work. The immunogenicity and induced protective immunity of MAP against Trichinella spiralis (T. spiralis) infection were evaluated in mice. We demonstrated that mice immunized with MAP containing CD4+ T cell and B cell epitopes (MAP-TB) induced significantly higher protection against the challenge of T. spiralis larvae (35.5% muscle larva reduction) compared to the MAP containing B cell epitope alone (MAP-B) with a 12.4% muscle larva reduction. The better protection induced by immunization of MAP-TB was correlated with boosted antibody titers (both IgG1 and IgG2a) and mixed Th1/Th2 cytokine production secreted by the splenocytes of immunized mice. Further flow cytometry analysis of lymphocytes in spleens and draining lymph nodes demonstrated that mice immunized with MAP-TB specifically enhanced the generation of T follicular helper (Tfh) cells and germinal center (GC) B cells, while inhibiting follicular regulatory CD4+ T (Tfr) cells and regulatory T (Treg) cells. Immunofluorescence staining of spleen sections also confirmed that MAP-TB vaccination enhanced the formation of GCs. Our results suggest that CD4+ T cell epitope of Ts-Pmy is crucial in vaccine component for inducing better protection against T. spiralis infection.
journal_name
J Immunol Resjournal_title
Journal of immunology researchauthors
Gu Y,Sun X,Huang J,Zhan B,Zhu Xdoi
10.1155/2020/2074803subject
Has Abstractpub_date
2020-01-08 00:00:00pages
2074803eissn
2314-8861issn
2314-7156journal_volume
2020pub_type
杂志文章abstract::An important function of the immune system is its ability to differentiate between healthy cells in the organism and "foreign" cells, allowing the latest to be attacked and the first ones to be conserved. The most important molecules in this process are considered to be checkpoint inhibitors. This review is focused on...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2018/4625472
更新日期:2018-10-17 00:00:00
abstract::Ischemia/reperfusion injury (IRI) remains a major problem in organ transplantation, which represents the main cause of graft dysfunction posttransplantation. Hepatic IRI is characterized by an excessive inflammatory response within the liver. Mesenchymal stem cells (MSCs) have been shown to be immunomodulatory cells a...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2018/7283703
更新日期:2018-12-03 00:00:00
abstract::Purified protein vaccines often require adjuvants for efficient stimulation of immune responses. There is no licensed mucosal adjuvant on the market to adequately boost the immune response to purified antigens for intranasal applications in humans. Bacterial outer membrane vesicles (OMV) are attractive candidates pote...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2016/3576028
更新日期:2016-01-01 00:00:00
abstract::Following fetal or neonatal gene transfer in mice and other species immune tolerance of the transgenic protein is frequently observed; however the underlying mechanisms remain largely undefined. In this study fetal and neonatal BALB/c mice received adenovirus vector to deliver human factor IX (hFIX) cDNA. The long-ter...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2015/397879
更新日期:2015-01-01 00:00:00
abstract::Bone disease in rheumatoid arthritis (RA) is a complex phenomenon where genetic risk factors have been partially evaluated. The system formed by receptor activator for nuclear factor-κB (RANK), receptor activator for nuclear factor-κB ligand (RANKL), and osteoprotegerin (OPG): RANK/RANKL/OPG is a crucial molecular pat...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2015/376197
更新日期:2015-01-01 00:00:00
abstract::We investigated cytokine production and expression of degranulation marker CD107a after different strategies of hepatitis B virus (HBV) vaccination in human immunodeficiency virus-infected individuals, which were three doses of 20 μg (standard dose group), four doses of 20 μg (four doses group), or four doses of 40 μg...
journal_title:Journal of immunology research
pub_type: 杂志文章,随机对照试验
doi:10.1155/2018/8350862
更新日期:2018-02-27 00:00:00
abstract::Recruitment of human NK cells to porcine tissues has been demonstrated in pig organs perfused ex vivo with human blood in the early 1990s. Subsequently, the molecular mechanisms leading to adhesion and cytotoxicity in human NK cell-porcine endothelial cell (pEC) interactions have been elucidated in vitro to identify t...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2017/4627384
更新日期:2017-01-01 00:00:00
abstract::Rheumatoid arthritis (RA) is a disease of chronic systemic inflammation (SI). In the present study, we used four datasets to explore whether methylation-derived neutrophil-to-lymphocyte ratio (mdNLR) might be a marker of SI in new onset, untreated, and treated prevalent RA cases and/or a marker of treatment response t...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2018/2624981
更新日期:2018-08-14 00:00:00
abstract::Bullous systemic lupus erythematosus (BSLE) is an autoantibody-mediated vesiculobullous disease in patients with SLE. Autoimmunity in BSLE is characterized by the presence of circulating anti-type VII collagen antibodies. BSLE patients often present with multiple, tense, clear fluid-filled vesicles and bullae overlyin...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2015/167064
更新日期:2015-01-01 00:00:00
abstract::The human leukocyte antigen-G (HLA-G) is considered an immune checkpoint molecule involved in tumor immune evasion. Hypoxia and the metabolic sensor hypoxia-inducible factor 1 (HIF-1) are hallmarks of metastasization, angiogenesis, and intense tumor metabolic activity. The purpose of this review was to examine origina...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2017/4587520
更新日期:2017-01-01 00:00:00
abstract::Antiphospholipid syndrome (APS) is characterized by development of venous and/or arterial thrombosis and pregnancy morbidity. Biological criteria are the persistent presence of lupus anticoagulant (LA) and/or anti-cardiolipin (aCL) and/or anti-B2GP1 autoantibodies' positivity. The assays' performances are of crucial i...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2015/983094
更新日期:2015-01-01 00:00:00
abstract::Chlorogenic acid (CA) is a phenolic compound commonly found in human plant-based diets. CA is the main component of many traditional Chinese medicine preparations, and in recent years, it has been found to have hypoglycemic, hypolipidemic, anti-inflammatory, antioxidant, and other pharmacological properties. Specifica...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2020/9680508
更新日期:2020-05-28 00:00:00
abstract::The phenotype and functional properties of antigen-presenting cells (APC), that is, circulating monocytes and generated in vitro macrophages and dendritic cells, were investigated in the patients with pulmonary tuberculosis (TB) differing in lymphocyte reactivity to M. tuberculosis antigens (PPD-reactive versus PPD-an...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2015/793292
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Systemic lupus erythematosus (SLE) is a multisystem disease that is characterized by the appearance of serum autoantibodies. No effective treatment for SLE currently exists. METHODS:We used human umbilical cord mesenchymal stem cell (H-UC-MSC) transplantation to treat B6.Fas mice. RESULTS:After four rounds...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2014/530501
更新日期:2014-01-01 00:00:00
abstract::Therapeutic monoclonal antibodies (mAbs) represent a milestone in pharmacological development. Their superiority is based on the combination of high specificity, low toxicity, and long half-life that characterizes biologics. If biologics have Achilles' heel, it is their potential immunogenicity. To better understand t...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2016/9096059
更新日期:2016-01-01 00:00:00
abstract:PURPOSES:To investigate the possible changes in B cell subsets and in B cell expression patterns of lipid rafts (LRs) and F-actin in patients with SLE and whether leflunomide treatment may have effect on these changes. METHODS:The B cell subsets and LRs expression were determined by flow cytometry and confocal microsc...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2015/832916
更新日期:2015-01-01 00:00:00
abstract::Systemic lupus erythematosus (SLE) is characterized by deregulated activation of T and B cells, autoantibody production, and consequent formation of immune complexes. Liposomes with nonbilayer phospholipid arrangements (NPA), induced by chlorpromazine, procainamide, or manganese, provoke a disease resembling human lup...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2017/8751642
更新日期:2017-01-01 00:00:00
abstract::Accumulating evidence has pointed out that metastasis is the leading cause of death in several malignant tumor, including CRC. During CRC, metastatic capacity is closely correlated with reprogrammed energy metabolism. Mitochondrial Pyruvate Carrier 1 (MPC1), as the carrier of transporting pyruvate into mitochondria, l...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2020/8340329
更新日期:2020-08-19 00:00:00
abstract::Bronchopulmonary dysplasia (BPD) is a severe complication of the respiratory system associated with preterm birth. Type 2 innate lymphoid cells (ILC2s) play a major role in tissue homeostasis, inflammation, and wound healing. However, the role in BPD remains unclear. The present study showed that ILC2s, interleukin-4 ...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2020/8050186
更新日期:2020-10-31 00:00:00
abstract::Adverse drug reactions (ADRs) remain a common and major problem in healthcare. Severe cutaneous adverse drug reactions (SCARs), such as Stevens-Johnson syndrome (SJS)/toxic epidermal necrolysis (TEN) with mortality rate ranges from 10% to more than 30%, can be life threatening. A number of recent studies demonstrated ...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2017/3186328
更新日期:2017-01-01 00:00:00
abstract::Human papillomavirus (HPV) is a small double-stranded DNA virus with tropism for epithelial cells. To this date, over 150 genotypes are known and are classified into two major groups, low-risk and high-risk strains, depending on the ability of the virus to induce malignant transformation. The host's immunity plays a c...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2018/5315816
更新日期:2018-08-27 00:00:00
abstract::Background. U65, a self-aggregating peptide scaffold, traps fused protein antigens in yeast cells. Conversion to Yeast Cell Particle (YCP) vaccines by partial removal of surface mannoproteins exposes β-glucan, mediating efficient uptake by antigen-presenting cells (APCs). YCP vaccines are inexpensive, capable of rapid...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2016/2743292
更新日期:2016-01-01 00:00:00
abstract::A derivative formula, DGBX, which is composed of three herbs (Radix astragali, Radix Angelicae sinensis, and Coptis chinensis Franch), is derived from a famous Chinese herbal formula, Danggui Buxue Tang (DBT) (Radix astragali and Radix Angelicae sinensis). We aimed to investigate the effects of DGBX on the regulation ...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2017/9786972
更新日期:2017-01-01 00:00:00
abstract::Oropouche virus (OROV) is an emerging pathogen which causes Oropouche fever and meningitis in humans. Several outbreaks of OROV in South America, especially in Brazil, have changed its status as an emerging disease, but no vaccine or specific drug target is available yet. Our approach was to identify the epitope-based...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2018/6718083
更新日期:2018-10-08 00:00:00
abstract::Calcitonin gene-related peptide (CGRP) is a mediator of microglial activation at the transcriptional level. The involvement of the epigenetic mechanism in this process is largely undefined. Histone deacetylase (HDAC)1/2 are considered important epigenetic regulators of gene expression in activated microglia. In this s...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2020/4384696
更新日期:2020-08-04 00:00:00
abstract::Inflammation plays an essential role in the control of pathogens and in shaping the ensuing adaptive immune responses. Traditionally, innate immunity has been described as a rapid response triggered through generic and nonspecific means that by definition lacks the ability to remember. Recently, it has become clear th...
journal_title:Journal of immunology research
pub_type: 杂志文章,评审
doi:10.1155/2018/1467538
更新日期:2018-06-11 00:00:00
abstract::Endotoxin shock is a life-threatening disorder, associated with the rapid release of neutrophil enzymes, including neutrophil collagenase/matrix metalloproteinase-8 (MMP-8) and gelatinase B/matrix metalloproteinase-9 (MMP-9). After activation, these enzymes cleave extracellular matrix components and cytokines and thus...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2014/747426
更新日期:2014-01-01 00:00:00
abstract::Type I interferon (IFN-I) plays a critical role in the antitumor immune response. In our previous study, we showed that IFN-I-inducible 2'-5' oligoadenylate synthetase-like 1 (OASL1) negatively regulated IFN-I production upon tumor challenge similar to that of viral infection. Thus, OASL1-deficient (Oasl1-/-) mice wer...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2019/7596786
更新日期:2019-03-31 00:00:00
abstract::In vitro differentiation of mesenchymal stromal cells (MSC) into osteocytes (human differentiated osteogenic cells, hDOC) before implantation has been proposed to optimize bone regeneration. However, a deep characterization of the immunological properties of DOC, including their effect on dendritic cell (DC) function,...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2015/526195
更新日期:2015-01-01 00:00:00
abstract::Regulatory T cells (Treg) can show plasticity whereby FOXP3 expression, the master transcription factor for Treg suppressor function, is lost and proinflammatory cytokines are produced. Optimal FOXP3 expression strongly depends on hypomethylation of the FOXP3 gene. 5-Azacytidine (Aza) and its derivative 5-aza-2'-deoxy...
journal_title:Journal of immunology research
pub_type: 杂志文章
doi:10.1155/2018/4973964
更新日期:2018-04-15 00:00:00