当前位置： SCI文献检索 > JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS期刊下所有文献 > Decoding active components in a formulation of multiple herbs for treatment of psoriasis based on three cell lines fishing and liquid chromatography-mass spectrometry analysis.

Decoding active components in a formulation of multiple herbs for treatment of psoriasis based on three cell lines fishing and liquid chromatography-mass spectrometry analysis.

Abstract：

:Chinese medicine formula (CMF) usually contains multiple herbs and its components are very complicated, which therapeutic effects are derived from the integrative activity of multiple components. Therefore, the screening of bioactive compounds in the formula is necessary, though it is very difficult, not only for elucidating the therapeutic components but also for developing the quality control method. In this work, three cell lines fishing combined with LC-MS analysis was developed for screening the potential bioactive compounds in Fuzhengzhiyanghefuzhiyang decoction (FZHFZY), a CMF for the treatment of psoriasis in clinic. Nineteen potential active compounds were discovered and thirteen of them were identified as rehmannioside D, melittoside or rehmannioside A, tufulingoside, neoastilbin, astilbin, neoisoastilbin, isoastilbin, engeletin, isoengeletin, spinosin, cnidimoside A, rutaevin and limonin, respectively. The effects of identified components were also analyzed in vitro. The results showed that IL-1β expression of RAW 264.7 cells treated with spinosin and melittoside as well as the TNF-α level of RAW 264.7 cells treated with rehmannioside D and melittoside were not significantly different compared with lipopolysaccharide (LPS)-treated cells. Though all identified potential active components had no apoptosis effect to HaCaT cells, rehmannioside D, rehmannioside A, astilbin and neoisoastilbin could significantly suppress HUVEC cells migration compared with control. These results indicated that the target cell fishing combined with LC-MS analysis is a useful tool for screening bioactive compounds from complicated CMF, and the identified active components may mainly contribute to the therapeutic effects of FZHFZY.

journal_name

J Pharm Biomed Anal

journal_title

Journal of pharmaceutical and biomedical analysis

authors

Chen L,Chen H,Lu Y,Han L,Wang S,Liu M,Li X,Zhao J,Lu C,Li S

doi

10.1016/j.jpba.2020.113331

subject

Has Abstract

pub_date

2020-07-15 00:00:00

pages

113331

eissn

0731-7085

issn

1873-264X

pii

S0731-7085(20)30521-5

journal_volume

186

pub_type

杂志文章

相关文献

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS文献大全
  • A colorimetric field method to assess the authenticity of drugs sold as the antimalarial artesunate.

    abstract：:Artesunate is the most widely used of the artemisinin derivatives. These drugs are being used increasingly throughout the tropical world, and are an essential component of the treatment of multi-drug resistant malaria. The recent and widespread appearance of counterfeit artesunate tablets in several countries in South...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/s0731-7085(00)00360-5

    authors： Green MD,Mount DL,Wirtz RA,White NJ

    更新日期：2000-12-01 00:00:00

  • Comparative pharmacokinetic study of the components in Alpinia oxyphylla Miq.-Schisandra chinensis (Turcz.) Baill. herb pair and its single herb between normal and Alzheimer's disease rats by UPLC-MS/MS.

    abstract：:Alzheimer's disease (AD) is a neurodegenerative disease that seriously affects daily life. Schisandra chinensis (Turcz.) Baill. Fructus (SCF) and Alpinia oxyphylla Miq. Fructus (AOF) have been regarded as classical herbs for dementia since ancient times. Alpinia oxyphylla Miq.-Schisandra chinensis (Turcz.) Baill. herb...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/j.jpba.2019.112874

    authors： Qi Y,Cheng X,Jing H,Yan T,Xiao F,Wu B,Bi K,Jia Y

    更新日期：2020-01-05 00:00:00

  • Method optimization of hydrophilic interaction chromatography separation of nucleotides using design of experiment approaches I: Comparison of several zwitterionic columns.

    abstract：:A systematic method in hydrophilic interaction chromatography (HILIC) was developed for the separation of four monophosphate nucleotides using design of experiment (DOE) approaches. Three HPLC parameters, the buffer concentration (ammonium acetate concentration), gradient time, and temperature, were evaluated within t...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/j.jpba.2018.05.014

    authors： Arase S,Kimura S,Ikegami T

    更新日期：2018-09-05 00:00:00

  • UHPLC-MS/MS method with automated on-line solid phase extraction for the quantification of entecavir in peripheral blood mononuclear cells of HBV+ patients.

    abstract：:To date five nucleoside analogs are used in the treatment of chronic hepatitis B: among these, entecavir is the most used. Nevertheless a few information about its distribution in tissues is currently known. Since the determination of entecavir disposition in the hepatocytes is impracticable because of its invasivenes...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/j.jpba.2015.10.017

    authors： De Nicolò A,Bonifacio G,Boglione L,Cusato J,Pensi D,Tomasello C,Di Perri G,D'Avolio A

    更新日期：2016-01-25 00:00:00

  • Determination of clotrimazole in mice plasma by capillary electrophoresis.

    abstract：:In addition to its antifungal activity, clotrimazole attracts interest as an anti-inflammatory drug. In order to correlate this effect with plasma concentrations in mice, a capillary electrophoretic method was developed. Sample preparation was carried out by protein precipitation using methanol. Quantification of clot...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/s0731-7085(02)00531-9

    authors： Wienen F,Laug S,Baumann K,Schwab A,Just S,Holzgrabe U

    更新日期：2003-01-15 00:00:00

  • Study of the British Pharmacopeia method on methimazole (thiamazole) content in carbimazole tablets.

    abstract：:The analysis of carbimazole tablets in The British Pharmacopoeia, 1993, includes a quantitative thin layer chromatography (TLC) determination of methimazole. Repeated analysis of the same samples did not give similar results. The repeatability and reproducibility of the method was studied. It was proved that the resid...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/s0731-7085(97)00119-2

    authors： Aletrari M,Kanari P,Partassides D,Loizou E

    更新日期：1998-01-01 00:00:00

  • Direct determination of s-carboxymethyl-l-cysteine in syrups by reversed-phase high-performance liquid chromatography.

    abstract：:A simple reversed-phase high-performance liquid chromatography method was developed for the determination of s-carboxymethyl-l-cysteine in syrup preparations. The experiments were performed without specific sample pre-treatment. The LC conditions used were acetonitrile-10 mM sodium dihydrogenphosphate buffer, pH 2.0 (...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/s0731-7085(00)00479-9

    authors： Suntornsuk L

    更新日期：2001-04-01 00:00:00

  • Simultaneous determination of methamphetamine and amphetamine in human urine using pipette tip solid-phase extraction and gas chromatography-mass spectrometry.

    abstract：:Methamphetamine and amphetamine were extracted from human urine samples using pipette tip solid-phase extraction (SPE) with MonoTip C18 tips (pipette tip volume, 200 microl), in which C18-bonded monolithic silica gel was fixed. A sample of human urine (0.5 ml) containing methamphetamine, amphetamine, and N-methylbenzy...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/j.jpba.2006.12.025

    authors： Kumazawa T,Hasegawa C,Lee XP,Hara K,Seno H,Suzuki O,Sato K

    更新日期：2007-06-28 00:00:00

  • Measurement of total and free docetaxel concentration in human plasma by ultra-performance liquid chromatography-tandem mass spectrometry.

    abstract：:Docetaxel is a semi-synthetic taxane with cytotoxic anti-neoplastic activity and, currently used as anticancer agent in several types of cancer. Docetaxel is highly bound to plasma proteins, and this significantly determines its clearance and activity. Therefore, measurement of free docetaxel in plasma is pharmacologi...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/j.jpba.2015.08.025

    authors： Rigo-Bonnin R,Cobo-Sacristán S,Gonzalo-Diego N,Colom H,Muñoz-Sánchez C,Urruticoechea A,Falo C,Alía P

    更新日期：2016-01-05 00:00:00

  • Voltammetric analysis of certain 4-quinolones in pharmaceuticals and biological fluids.

    abstract：:The voltammetric behaviour of Enrofloxacin (I), Sparfloxacin (II) and Fleroxacin (III) was studied using direct current (DCt), differential pulse (DPP) and alternating current (ACt). All the drugs manifest cathodic waves in Britton Robinson buffer over the pH range of 4.0-11.98. The waves were characterized as being i...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/s0731-7085(00)00401-5

    authors： Rizk M,Belal F,Ibrahim F,Ahmed S,El-Enany NM

    更新日期：2000-12-15 00:00:00

  • Purification of [18F]-fluoro-L-thymidine ([18F]-FLT) for positron emission tomography imaging.

    abstract：:3'-Deoxy-3'-[18F]fluorothymidine ([18F]-FLT) has recently been described as a positron emission tomography (PET) radiopharmaceutical for visualizing cellular proliferation in vivo. All published radiosyntheses of [18F]-FLT provide crude products that must be purified before injection to human. This study describes a r...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/j.jpba.2007.04.034

    authors： Bourgeois M,Mougin-Degraef M,Leost F,Cherel M,Gestin JF,Le Bars D,Barbet J,Faivre-Chauvet A

    更新日期：2007-09-21 00:00:00

  • Determination of porcine fibrinogen in rat and dog plasma after intraperitoneal injection of a porcine-derived fibrin glue by fluorescein-labeled assay method: Comparison with isotope-labeled assay method.

    abstract：:A sensitive and specific fluorescein isothiocyanate (FITC) label coupled with size-exclusion high-performance liquid chromatography-fluorescence detection (SE-HPLC-FLD) method was developed and validated for the estimation of the pharmacokinetic profiles of porcine fibrinogen after intraperitoneal injection of a porci...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/j.jpba.2011.08.037

    authors： Xie Y,He H,Fan G,Wu Y

    更新日期：2012-01-05 00:00:00

  • LC determination of the enantiomeric purity of levamisole using stationary phase with bonded naphthylethylcarbamoylated-beta-cyclodextrin.

    abstract：:A direct enantioselective high-performance liquid chromatography was employed successfully for determination of the enantiomeric purity of levamisole. The elaborated method used S-naphthylethylcarbamoylated beta-cyclodextrin stationary phase in reversed-phase mode. The optimized mobile phase composition was acetonitri...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/s0731-7085(00)00513-6

    authors： Dolezalová M,Tkaczyková M

    更新日期：2001-06-01 00:00:00

  • Improved sample preparation method for fast LC-MS/MS analysis of vitamin D metabolites in serum.

    abstract：:Despite the fact that more than 90% of vitamin D analysis are performed using immuno-enzymatic techniques, it is liquid chromatography coupled with tandem mass spectrometry that is currently the reference method. It allows for specific and selective analysis of all relevant vitamin D metabolites from a variety of biol...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/j.jpba.2020.113529

    authors： Rola R,Kowalski K,Bieńkowski T,Studzińska S

    更新日期：2020-10-25 00:00:00

  • Validated HPLC determination of 2-[(dimethylamino)methyl]cyclohexanone, an impurity in tramadol, using a precolumn derivatisation reaction with 2,4-dinitrophenylhydrazine.

    abstract：:A new method for the determination of 2-[(dimethylamino)methyl]cyclohexanone (DAMC) in Tramadol (as active substance or active ingredient in pharmaceutical formulations) is described. The method is based on the derivatisation of 2-[(dimethylamino)methyl]cyclohexanone with 2,4-dinitrophenylhydrazine (2,4-DNPH) in acidi...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/j.japna.2003.07.003

    authors： Medvedovici A,Albu F,Farca A,David V

    更新日期：2004-01-27 00:00:00

  • Intact charge variant analysis of ziv-aflibercept by cationic exchange liquid chromatography as a proof of concept: Comparison between volatile and non-volatile salts in the mobile phase.

    abstract：:Ziv-aflibercept (ziv-AFL) is a complex fusion protein which is widely used in hospitals for the treatment of colorectal metastatic cancer. Charge variants are critical attributes for assessing post-transitional modifications (PMTs) that have to be controlled during the development and manufacture of these proteins and...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/j.jpba.2020.113233

    authors： Pérez-Robles R,Cuadros-Rodríguez L,Salmerón-García A,Cabeza-Barrera J,Navas N

    更新日期：2020-06-05 00:00:00

  • Oxidation of diclofenac in water by sodium hypochlorite: Identification of new degradation by-products and their ecotoxicological evaluation.

    abstract：:Diclofenac (DCF) is the most widely prescribed non-steroidal anti-inflammatory drug in the world and it has been detected in drinking and surface waters. In this paper, the effect of chlorination process on DCF in aqueous solutions was investigated and the structures of 14 isolated degradation by-products (DPs), of wh...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/j.jpba.2020.113762

    authors： Luongo G,Guida M,Siciliano A,Libralato G,Saviano L,Amoresano A,Previtera L,Di Fabio G,Zarrelli A

    更新日期：2021-02-05 00:00:00

  • Characterization of three main degradation products from novel oral anticoagulant rivaroxaban under stress conditions by UPLC-Q-TOF-MS/MS.

    abstract：:Drugs of long-term use may cause the accumulation of chemical compounds in human body. Therefore, the evaluation and structure characterization of synthesis and degradation impurities is substantial to guarantee drug safety and successful pharmaceutical therapy. The present work evaluated the anticoagulant rivaroxaban...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/j.jpba.2016.01.053

    authors： Wingert NR,dos Santos NO,Nunes MA,Gomes P,Müller EI,Flores ÉM,Steppe M

    更新日期：2016-05-10 00:00:00

  • Development and full validation of an UPLC-MS/MS method for the quantification of the plant-derived alkaloid indirubin in rat plasma.

    abstract：:An UPLC-MS/MS method for the quantification of indirubin in lithium heparinized rat plasma was developed and validated according to current international guidelines. Indirubin was extracted from rat plasma by using Waters Ostro™ pass-through sample preparation plates. The method was validated with a LLOQ of 5.00ng/mL ...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/j.jpba.2016.05.018

    authors： Jähne EA,Sampath C,Butterweck V,Hamburger M,Oufir M

    更新日期：2016-09-05 00:00:00

  • Adsorptive cathodic stripping voltammetric assay of the estrogen drug ethinylestradiol in pharmaceutical formulation and human plasma at a mercury electrode.

    abstract：:The electroreduction of ethinylestradiol at the hanging mercury drop electrode in the Britton-Robinson universal buffer of pH 2-11 was studied and its interfacial adsorptive character onto the mercury electrode surface was identified. A validated simple, rapid, sensitive, specific, precise and inexpensive square-wave ...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/j.jpba.2005.07.024

    authors： Ghoneim EM,El-Desoky HS,Ghoneim MM

    更新日期：2006-02-13 00:00:00

  • Rapid liquid chromatographic assay of ciprofloxacin in human aqueous humor.

    abstract：:A simple, selective and sensitive method has been developed to determine ciprofloxacin in human aqueous humor. Separation of ciprofloxacin was carried out with pipemidic acid as internal standard using a Novapak C18 reversed-phase cartridge column (100 x 8 mm i.d., particle size 4 microns) and a mobile phase consistin...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 临床试验,杂志文章

    doi：10.1016/0731-7085(95)01614-7

    authors： Basci NE,Bozkurt A,Kalayci D,Kayaalp SO

    更新日期：1996-01-01 00:00:00

  • Structural characterization of dimethyldithiodenafil and dimethylthiocarbodenafil, analogs of sildenafil.

    abstract：:Two analogs of sildenafil (compounds 1 and 2) were detected in three powder products acquired from online drug markets during an LC-UV-MS analysis of psychotropic drugs. Their structures were established by high-resolution mass spectrometry and NMR spectroscopy. Compound 1 was identified as 5-(5-(3,5-dimethylpiperazin...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/j.jpba.2017.09.025

    authors： Sakamoto M,Suzuki J,Saito Y,Shimizu S,Kobayashi K,Nagashima M,Moriyasu T,Fukaya H,Saito K

    更新日期：2018-01-30 00:00:00

  • Simultaneous quantification of cefepime, meropenem, ciprofloxacin, moxifloxacin, linezolid and piperacillin in human serum using an isotope-dilution HPLC-MS/MS method.

    abstract：:The aim of the current study was to develop and validate a robust multi-analyte high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method for simultaneous quantification of cefepime, meropenem, ciprofloxacin, moxifloxacin, linezolid and piperacillin, which are the most commonly used antibioti...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/j.jpba.2018.01.031

    authors： Paal M,Zoller M,Schuster C,Vogeser M,Schütze G

    更新日期：2018-04-15 00:00:00

  • Determination of lovastatin in human plasma by ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry and its application in a pharmacokinetic study.

    abstract：:A selective, rapid and sensitive ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for the quantitative determination of lovastatin in human plasma and its application in a pharmacokinetic study. With mycophenolate mofetil as internal standard, sample pretreatment invol...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/j.jpba.2007.12.005

    authors： Yuan H,Wang F,Tu J,Peng W,Li H

    更新日期：2008-03-13 00:00:00

  • A bioassay for the detection of neutralizing antibodies against the α-melanocyte stimulating hormone analog afamelanotide in patients with erythropoietic protoporphyria.

    abstract：:The tridecapeptide afamelanotide (Scenesse®) is a congener of α-melanocyte stimulating hormone (α-MSH). Upon binding to the melanocortin 1 receptor (MC1R) on the surface of pigment cells of the skin, the melanocytes, α-MSH or afamelanotide trigger the synthesis of cAMP, which stimulates the synthesis of melanin and th...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/j.jpba.2012.11.040

    authors： Spichty R,Balimann M,Barman J,Minder EI

    更新日期：2013-03-05 00:00:00

  • Liquidambar styraciflua L.: A new potential source for therapeutic uses.

    abstract：:Liquidambar styraciflua L., ALTINGIACEAE, popularly known as sweet gum or alligator tree, is an aromatic tree with a natural distribution in North America and acclimated in Brazil. In traditional medicine, L. styraciflua L is used for the treatment of stomach disorders, wounds, and coughs. The present study was design...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/j.jpba.2019.06.003

    authors： Mancarz GFF,Laba LC,da Silva ECP,Prado MRM,de Souza LM,de Souza D,Nakashima T,Mello RG

    更新日期：2019-09-10 00:00:00

  • Development and validation of LC-MS/MS method for the detection and quantification of CpG oligonucleotides 107 (CpG ODN107) and its metabolites in mice plasma.

    abstract：:CpG oligodeoxynucleotide 107 (CpG ODN107) could be used as a novel radiosensitizer for glioma. Herein, a novel and sensitive reversed-phase HPLC coupled with electrospray triple quadrupole mass spectrometry (LC-MS/MS) following a one-step C18 solid-phase extraction (SPE) for biological matrix removal was developed and...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/j.jpba.2012.06.022

    authors： Cen Y,Li X,Liu D,Pan F,Cai Y,Li B,Peng W,Wu C,Jiang W,Zhou H

    更新日期：2012-11-01 00:00:00

  • Analytical approach for the determination of steroid profile of humans by gas chromatography isotope ratio mass spectrometry aimed at distinguishing between endogenous and exogenous steroids.

    abstract：:The contamination of commonly used supplements by unknown steroids as well as their metabolites (parent compounds) become a challenge for the analytical laboratories. Although the determination of steroids profile is not trivial because of the complex matrix and low concentration of single compound, one of the most di...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/j.jpba.2014.11.017

    authors： Bulska E,Gorczyca D,Zalewska I,Pokrywka A,Kwiatkowska D

    更新日期：2015-03-15 00:00:00

  • Preparation and evaluation of kaempferol-phospholipid complex for pharmacokinetics and bioavailability in SD rats.

    abstract：:As one of the dietary flavonoids, kaempferol (KP) has been well known to show strong anti-oxidative effect along with other biological properties. However, the oral bioavailability of KP is relatively low due to its poor solubility. In this study, we intended to increase the solubility and bioavailability of KP by pre...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/j.jpba.2015.05.017

    authors： Zhang K,Gu L,Chen J,Zhang Y,Jiang Y,Zhao L,Bi K,Chen X

    更新日期：2015-10-10 00:00:00

  • Simultaneous determination of four bioactive constituents in Liuwei Dihuang Pills by micellar electrokinetic chromatography.

    abstract：:A micellar electrokinetic chromatography (MEKC) method for the simultaneous determination of four bioactive constituents (morroniside, loganin, paeoniflorin and paeonal) in the Chinese patent medicine Liuwei Dihuang Pills is established. A carrier composed of 0.2M boric acid, 0.02 M sodium dodecyl sulfate (SDS) and 5%...

    journal_title：Journal of pharmaceutical and biomedical analysis

    pub_type： 杂志文章

    doi：10.1016/j.jpba.2007.04.025

    authors： Zhao X,Wang Y,Sun Y

    更新日期：2007-09-03 00:00:00