Abstract:
:Coronavirus disease 2019 (COVID-19), caused by the novel human coronavirus SARS-CoV-2, is currently a major threat to public health worldwide. The viral spike protein binds the host receptor angiotensin-converting enzyme 2 (ACE2) via the receptor-binding domain (RBD), and thus is believed to be a major target to block viral entry. Both SARS-CoV-2 and SARS-CoV share this mechanism. Here we functionally analyzed the key amino acid residues located within receptor binding motif of RBD that may interact with human ACE2 and available neutralizing antibodies. The in vivo experiments showed that immunization with either the SARS-CoV RBD or SARS-CoV-2 RBD was able to induce strong clade-specific neutralizing antibodies in mice; however, the cross-neutralizing activity was much weaker, indicating that there are distinct antigenic features in the RBDs of the two viruses. This finding was confirmed with the available neutralizing monoclonal antibodies against SARS-CoV or SARS-CoV-2. It is worth noting that a newly developed SARS-CoV-2 human antibody, HA001, was able to neutralize SARS-CoV-2, but failed to recognize SARS-CoV. Moreover, the potential epitope residues of HA001 were identified as A475 and F486 in the SARS-CoV-2 RBD, representing new binding sites for neutralizing antibodies. Overall, our study has revealed the presence of different key epitopes between SARS-CoV and SARS-CoV-2, which indicates the necessity to develop new prophylactic vaccine and antibody drugs for specific control of the COVID-19 pandemic although the available agents obtained from the SARS-CoV study are unneglectable.
journal_name
Cell Mol Immunoljournal_title
Cellular & molecular immunologyauthors
Yi C,Sun X,Ye J,Ding L,Liu M,Yang Z,Lu X,Zhang Y,Ma L,Gu W,Qu A,Xu J,Shi Z,Ling Z,Sun Bdoi
10.1038/s41423-020-0458-zsubject
Has Abstractpub_date
2020-06-01 00:00:00pages
621-630issue
6eissn
1672-7681issn
2042-0226pii
10.1038/s41423-020-0458-zjournal_volume
17pub_type
杂志文章abstract::B cells play an important role in the clearance of hepatitis B virus (HBV) and protection against reinfection. However, the functional characteristics of these cells that are associated with the outcome of chronic HBV infection remain unknown. We comprehensively investigated the frequency, phenotype, and function of p...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章
doi:10.1038/cmi.2015.25
更新日期:2015-05-01 00:00:00
abstract::Recombinant human prolactin (rhPRL) was administered to huPBL-SCID mice to determine its effects on human immunologic reconstitution and function. The huPBL-SCID mice were given 10 microg i.p. injection of rhPRL every other day for a total of 10 injections after huPBL were transferred. The results demonstrated that rh...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章
doi:
更新日期:2004-04-01 00:00:00
abstract::Hypoxia is a major characteristic of the tumor microenvironment, and its effects on immune cells are proposed to be important factors for the process of tumor immune escape. It has been reported that hypoxia affects the function of dendritic cells and the antitumor function of T cells. Here we discuss the effects of h...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章
doi:10.1038/cmi.2009.105
更新日期:2010-01-01 00:00:00
abstract::Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been redetected after discharge in some coronavirus disease 2019 (COVID-19) patients. The reason for the recurrent positivity of the test and the potential public health concern due to this occurrence are still unknown. Here, we analyzed the viral data a...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章
doi:10.1038/s41423-020-00550-2
更新日期:2020-11-01 00:00:00
abstract::Follicular helper T (Tfh) cells select mutated B cells in germinal centres, which can then differentiate into long-lived high affinity memory B cells and plasma cells. Tfh cells are regulated by a unique molecular programme orchestrated by the transcriptional repressor Bcl6. This transcription factor turns down expres...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章,评审
doi:10.1038/cmi.2010.18
更新日期:2010-05-01 00:00:00
abstract::Innate lymphoid cells (ILCs), including natural killer (NK) cells, ILC1s, ILC2s, ILC3s, and lymphoid tissue inducer (LTi) cells, comprise the first line of innate immune defense against pathogens and tumors. Over the past decade, accumulating evidence has demonstrated immunological memory in ILC subsets: for example, ...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章,评审
doi:10.1038/s41423-019-0212-6
更新日期:2019-05-01 00:00:00
abstract::We demonstrate that Mycobacterium tuberculosis recombinant leucine-responsive regulatory protein (rLrp) inhibits lipopolysaccharide (LPS)-induced tumor necrosis factor alpha (TNF-α), interleukin-6, and interleukin-12 production and blocks the nuclear translocation of subunits of the nuclear-receptor transcription fact...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章
doi:10.1038/cmi.2015.58
更新日期:2016-11-01 00:00:00
abstract::Some pathogens can use host suppressor of cytokine signaling 1 (SOCS-1), an important negative-feedback molecule, as the main mode of immune evasion. Here we found that group A Streptococcus (GAS) is capable of inducing SOCS-1 expression in RAW264.7 and BMDM macrophages. IFN-β plays a role in GAS-induced SOCS-1 expres...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章
doi:10.1038/cmi.2014.107
更新日期:2015-05-01 00:00:00
abstract::The disturbance of maternal immune tolerance to a semiallogeneic fetus is recognized as one of the key pathologies of preeclampsia (PE), in which an imbalance between the inflammation-limiting regulatory T cells (Tregs) and the inflammation-mediating Th17 cells plays an essential role. Previously, we reported that the...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章
doi:10.1038/s41423-018-0186-9
更新日期:2019-01-01 00:00:00
abstract::Organ transplantation is an effective therapeutic tool for treating many terminal diseases. However, one of the biggest challenges of transplantation is determining how to achieve the long-term survival of the allogeneic or xenogeneic transplant by, for example, preventing transplant rejection. In the current study, C...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章
doi:10.1038/s41423-018-0009-z
更新日期:2019-06-01 00:00:00
abstract::Exercise at regular intervals is assumed to have a positive effect on immune functions. Conversely, after spaceflight and under simulated weightlessness (e.g., bed rest), immune functions can be suppressed. We aimed to assess the effects of simulated weightlessness (Second Berlin BedRest Study; BBR2-2) on immunologica...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章,随机对照试验
doi:10.1038/cmi.2014.106
更新日期:2015-07-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Cellular & molecular immunology
pub_type: 已发布勘误
doi:10.1038/s41423-020-00543-1
更新日期:2020-10-01 00:00:00
abstract::The human immunodeficiency virus-1 (HIV-1) envelope protein gp120 is the major contributor to the pathogenesis of HIV-associated neurocognitive disorder (HAND). Neuroinflammation plays a pivotal role in gp120-induced neuropathology, but how gp120 triggers neuroinflammatory processes and subsequent neuronal death remai...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章
doi:10.1038/s41423-019-0260-y
更新日期:2020-03-01 00:00:00
abstract::Allergen-specific immunotherapy (allergen-SIT) is a highly effective treatment for children with allergic asthma (AA), an immune-mediated chronic disease leading to bronchial muscle hypertrophy and airway obstruction in response to specific allergens. T helper cells and secreted cytokines play important roles in the p...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章
doi:10.1038/cmi.2017.26
更新日期:2018-11-01 00:00:00
abstract::Viral RNAs produced during viral infection are recognized by the cytoplasmic RNA helicases retinoic acid-inducible gene-I (RIG-I) and melanoma differentiation-associated gene 5 (MDA5). A central adapter protein downstream of RIG-I and MDA5 is the mitochondrial membrane protein virus-induced signaling adaptor (VISA), w...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章
doi:10.1038/cmi.2010.36
更新日期:2010-09-01 00:00:00
abstract::Viral encephalitis is a devastating disease with high mortality, and survivors often suffer from severe neurological complications. Microglia are innate immune cells of the central nervous system (CNS) parenchyma whose turnover is reliant on local proliferation. Microglia express a diverse range of proteins, which all...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章,评审
doi:10.1038/s41423-020-00620-5
更新日期:2021-02-01 00:00:00
abstract::The nuclear receptor superfamily and the transcriptional factors associated with cytokines are inherently different families of signaling molecules and activate gene transcription by binding to their respective responsive element. However, it has become increasingly clear from our works and others that nuclear recepto...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章,评审
doi:
更新日期:2004-12-01 00:00:00
abstract::Dendritic cells are powerful antigen-presenting cells that are essential for the priming of T cell responses. In addition to providing T-cell-receptor ligands and co-stimulatory molecules for naive T cell activation and expansion, dendritic cells are thought to also provide signals for the differentiation of CD4+ T ce...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章,评审
doi:10.1038/s41423-020-0465-0
更新日期:2020-06-01 00:00:00
abstract:OBJECTIVE:Plasma phospholipid transfer protein (PLTP) is a key determinant of lipoprotein metabolism, and both animal and human studies converge to indicate that PLTP promotes atherogenesis and its thromboembolic complications. Moreover, it has recently been reported that PLTP modulates inflammation and immune response...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章
doi:10.1038/cmi.2015.75
更新日期:2016-11-01 00:00:00
abstract::A complex process including both the innate and acquired immune responses results in allograft rejection. Some chemokine receptors and their ligands play essential roles not only for leukocyte migration into the graft but also in facilitating dendritic and T cell trafficking between lymph nodes and the transplant in t...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章,评审
doi:
更新日期:2005-10-01 00:00:00
abstract::Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by B-cell hyperreactivity. The Toll-like receptor 7 (TLR7) signaling pathway is abnormally activated in SLE B cells. CyclinD3 (CCND3) plays an important role in B-cell proliferation, development, and differentiation. Although previous studies fo...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章
doi:10.1038/cmi.2015.48
更新日期:2016-11-01 00:00:00
abstract::Induction of osteopontin (OPN), a well-known pro-inflammatory molecule, has been observed in acetaminophen (APAP)-induced hepatotoxicity. However, the precise cell source for OPN induction and its role during APAP-induced hepatotoxicity has not been fully explored. By employing a hepatotoxic mouse model induced by APA...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章
doi:10.1038/s41423-018-0033-z
更新日期:2019-05-01 00:00:00
abstract::pR(ST98) is a large and conjugative resistant plasmid (R plasmid) of 98.6 mega-dalton from multi-drug resistant Salmonella typhi (S. typhi), which was classified to incompatibility group C (Inc C). It has been found that pRST98 made its host bacteria not only antibiotic resistant but also more virulent. In this study ...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章
doi:
更新日期:2005-04-01 00:00:00
abstract::The major limitation for the maturation of dendritic cells (DCs) using Toll-like receptor (TLR) agonists is their decreased ability to migrate into lymph nodes compared with conventional DCs. CD38 can be used as a multifunctional marker to modulate migration, survival and Th1 responses of DCs. CD74 has been shown to n...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章
doi:10.1038/cmi.2011.7
更新日期:2011-07-01 00:00:00
abstract::Efficient immune responses against invading pathogens often involve coordination between cells from both the innate and adaptive immune systems. For multiple decades, it has been believed that CD8+ memory T cells and natural killer (NK) cells constantly and uniformly recirculate. Only recently was the existence of non...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章,评审
doi:10.1038/s41423-018-0192-y
更新日期:2019-03-01 00:00:00
abstract::B cells are generally considered to be positive regulators of the immune response because of their capability to produce antibodies, including autoantibodies. The production of antibodies facilitates optimal CD4(+) T-cell activation because B cells serve as antigen-presenting cells and exert other modulatory functions...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章,评审
doi:10.1038/cmi.2012.60
更新日期:2013-03-01 00:00:00
abstract::Patients with primary ciliary dyskinesia (PCD) and cystic fibrosis (CF), two inherited disorders, suffer from recurrent airway infections characterized by persistent bacterial colonization and uncontrollable inflammation. Although present in high counts, neutrophils fail to clear infection in the airways. High levels ...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章,评审
doi:10.1038/cmi.2017.118
更新日期:2018-04-01 00:00:00
abstract::Inflammatory bowel disease (IBD) is an important factor in the induction of colon cancer, but its mechanism is unclear. Colitis and colitis-associated colorectal cancer (CAC) models induced using both dextran sulfate sodium (DSS) and the azoxymethane/DSS protocol were established in wild-type (WT) and CTRP4 transgenic...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章
doi:10.1038/cmi.2016.16
更新日期:2016-09-01 00:00:00
abstract::Neutrophil infiltration is a hallmark of alcoholic steatohepatitis; however, the underlying mechanisms remain unclear. We previously reported that chronic-plus-binge ethanol feeding synergistically induces hepatic recruitment of neutrophils, which contributes to liver injury. In this paper, we investigated the roles o...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章
doi:10.1038/cmi.2015.06
更新日期:2016-03-01 00:00:00
abstract::Appropriate in vivo control of plasmacytoid dendritic cell (pDC) recruitment and activation is a fundamental requirement for defense against viral infection. During this process, a pivotal event that influences the outcome of viral infection is the production of high levels of type I interferon by pDCs. In particular,...
journal_title:Cellular & molecular immunology
pub_type: 杂志文章,评审
doi:
更新日期:2005-12-01 00:00:00