Photoaged Skin Therapy with Adipose-Derived Stem Cells.

Abstract:

BACKGROUND:The major intrinsic cause of facial skin degeneration is age, associated with extrinsic factors such as exposure to sun. Its major pathologic causes are degeneration of the elastin matrix, with loss of oxytalan and elaunin fibers in the subepidermal region, and actinic degeneration of elastin fibers that lose their functional properties in the deep dermis. Therapy using autologous adipose mesenchymal stem cells for regeneration of extracellular matrix in patients with solar elastosis was addressed in qualitative and quantitative analyses of the dermal elastic fiber system and the associated cells. METHODS:Mesenchymal stem cells were obtained from lipoaspirates, expanded in vitro, and introduced into the facial skin of patients submitted after 3 to 4 months to a face-lift operation. In the retrieved skin, immunocytochemical analyses quantified elastic matrix components; cathepsin K; matrix metalloproteinase 12 (macrophage metalloelastase); and the macrophage M2 markers CD68, CD206, and hemeoxygenase-1. RESULTS:A full de novo formation of oxytalan and elaunin fibers was observed in the subepidermal region, with reconstitution of the papillary structure of the dermal-epidermal junction. Elastotic deposits in the deep dermis were substituted by a normal elastin fiber network. The coordinated removal of the pathologic deposits and their substitution by the normal ones was concomitant with activation of cathepsin K and matrix metalloproteinase 12, and with expansion of the M2 macrophage infiltration. CONCLUSION:The full regeneration of solar elastosis was obtained by injection of in vitro expanded autologous adipose mesenchymal stem cells, which are appropriate, competent, and sufficient to elicit the full structural regeneration of the sun-aged skin. CLINICAL QUESTION/LEVEL OF EVIDENCE:Therapeutic, IV.

journal_name

Plast Reconstr Surg

authors

Charles-de-Sá L,Gontijo-de-Amorim NF,Rigotti G,Sbarbati A,Bernardi P,Benati D,Bizon Vieira Carias R,Maeda Takiya C,Borojevic R

doi

10.1097/PRS.0000000000006867

subject

Has Abstract

pub_date

2020-06-01 00:00:00

pages

1037e-1049e

issue

6

eissn

0032-1052

issn

1529-4242

pii

00006534-202006000-00012

journal_volume

145

pub_type

临床试验,杂志文章
  • The effect of preoperative angiography on experimental free-flap survival.

    abstract::In a rat model designed to simulate clinical angiography, preoperative angiography had no effect on free-flap survival. Only when a large dose of RCM was delivered directly into a surgically isolated circulation did significant free-flap loss ensue. ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-198108000-00013

    authors: Yaremchuk MJ,Bartlett SP,Sedacca T,May JW Jr

    更新日期:1981-08-01 00:00:00

  • Sweet's syndrome presenting as acute hand infection.

    abstract:BACKGROUND:Sweet's syndrome, originally described as an acute febrile neutrophilic dermatosis, belongs to a class of skin lesions that histologically have intense epidermal and/or dermal inflammatory infiltrate of neutrophils without evidence of infection or vasculitis. Skin lesions of Sweet's syndrome most commonly pr...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/01.prs.0000172890.53979.09

    authors: O'Halloran LE,Bennett LL,Grothaus PC,Lynch DJ

    更新日期:2005-08-01 00:00:00

  • Changing the convexity and concavity of nasal cartilages and cartilage grafts with horizontal mattress sutures: part II. Clinical results.

    abstract::Horizontal mattress sutures have previously been shown to remove unwanted bulbosity and convexity of nasal tip cartilages. The purpose of this study was to extend that concept by investigating the universal applicability of the horizontal mattress suture to change and control the curvature (e.g., convexity or concavit...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/01.prs.0000150146.04465.81

    authors: Gruber RP,Nahai F,Bogdan MA,Friedman GD

    更新日期:2005-02-01 00:00:00

  • The use of epidurals in abdominal wall reconstruction: an analysis of outcomes and cost.

    abstract:BACKGROUND:Ventral hernias are a common, challenging, and expensive problem for general and reconstructive surgeons. The authors assessed the impact of epidurals on morbidity following abdominal wall reconstruction for hernia. METHODS:A retrospective review of abdominal wall reconstruction patients operated on between...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/01.prs.0000438048.91139.31

    authors: Fischer JP,Nelson JA,Wes AM,Wink JD,Yan C,Braslow BM,Chen L,Kovach SJ

    更新日期:2014-03-01 00:00:00

  • Selection of topical antimicrobial agents for cultured skin for burns by combined assessment of cellular cytotoxicity and antimicrobial activity.

    abstract::Cultured epidermal skin has become an adjunctive therapy for treatment of major burn injuries, but its effectiveness is greatly limited due to destruction by microbial contamination. To evaluate candidate drugs for use with cultured skin, a combined cytotoxicity-antimicrobial assay system was developed for determinati...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-199309000-00018

    authors: Boyce ST,Holder IA

    更新日期:1993-09-01 00:00:00

  • Osteogenesis in calvarial defects: contribution of the dura, the pericranium, and the surrounding bone in adult versus infant animals.

    abstract::Guided bone regeneration is a promising means for reconstructing bone defects in the cranium. The present study was performed to better define those factors that affect osteogenesis in the cranium. The authors studied a single animal model, investigating the contribution of the dura, the pericranium, and the adjacent ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/01.PRS.0000070728.56716.51

    authors: Gosain AK,Santoro TD,Song LS,Capel CC,Sudhakar PV,Matloub HS

    更新日期:2003-08-01 00:00:00

  • Double-paddle peroneal tissue transfer for oromandibular reconstruction.

    abstract::The double-paddle peroneal tissue transfer is a useful technique for reconstructing the extensive and complex defect that results after ablative surgery for oral cancer. It can facilitate the design and inset of the skin paddle and avoid the need for a second free flap. The two skin paddles can be based on either two ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-200007000-00008

    authors: Yang KC,Leung JK,Chen JS

    更新日期:2000-07-01 00:00:00

  • Urokinase protocol for free-flap salvage following prolonged venous thrombosis.

    abstract::The incidence of free-flap failure is reported at 4 to 5 percent. Often, these failures are attributed to postoperative venous thrombosis with salvage rates reported at 42 percent. The use of thrombolytics has been effective in laboratory protocols; however, there have been only case reports to substantiate their use ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-199811000-00021

    authors: Serletti JM,Moran SL,Orlando GS,O'Connor T,Herrera HR

    更新日期:1998-11-01 00:00:00

  • Surgical management of penoscrotal lymphangioma circumscriptum.

    abstract::Lymphangioma circumscriptum of the penis and scrotum is an unusual entity that may be indistinguishable from genital warts. After confirmation of the diagnosis, a treatment plan consisting of wide excision should be outlined. To lower the chance of recurrence, not only the affected skin but all the subjacent subcutane...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-199901000-00028

    authors: Latifoğlu O,Yavuzer R,Demir Y,Ayhan S,Yenidünya S,Atabay K

    更新日期:1999-01-01 00:00:00

  • Tolerance induction in composite facial allograft transplantation in the rat model.

    abstract::Clinical application of composite tissue allograft transplants opened discussion on the restoration of facial deformities by allotransplantation. The authors introduce a hemifacial allograft transplant model to investigate the rationale for the development of functional tolerance across the major histocompatibility co...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/01.prs.0000142414.92308.ab

    authors: Demir Y,Ozmen S,Klimczak A,Mukherjee AL,Siemionow M

    更新日期:2004-12-01 00:00:00

  • Analyzing Regional Differences over a 15-Year Trend of One-Stage versus Two-Stage Breast Reconstruction in 941,191 Postmastectomy Patients.

    abstract:BACKGROUND:Implant-based reconstruction is the predominant form of breast reconstruction, with the two-stage tissue expander/implant approach being the most popular. Recently, the direct-to-implant, one-stage breast reconstruction procedure has gained momentum. In this study, national and regional trends across the Uni...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/PRS.0000000000002267

    authors: Kamali P,Koolen PGL,Ibrahim AMS,Paul MA,Dikmans RE,Schermerhorn ML,Lee BT,Lin SJ

    更新日期:2016-07-01 00:00:00

  • Alar rim excision: a method of thinning bulky nostrils.

    abstract::A thick nostril is a disturbing deformity and one for which surgeons and patients may be reluctant to employ the traditional "piece of pie" skin excisions commonly described. As an alternative in selected circumstances, a method of internally thinning the nostril rim using an alar base groove incision known as alar ri...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-199604000-00023

    authors: Matarasso A

    更新日期:1996-04-01 00:00:00

  • A comparison of factors affecting aesthetic outcomes of TRAM flap breast reconstructions.

    abstract::The aesthetic results of TRAM flap breast reconstructions were assessed in 237 consecutive breast cancer patients. Nine independent judges, none of whom were plastic surgeons or members of the reconstructive team, assigned an aesthetic score to each patient after viewing postoperative photographs. We found that bilate...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-199509001-00015

    authors: Kroll SS,Coffey JA Jr,Winn RJ,Schusterman MA

    更新日期:1995-09-01 00:00:00

  • Comparison of innervated and noninnervated free flaps in oral reconstruction.

    abstract::Thirteen patients who had undergone ablative surgery for advanced squamous cell carcinoma in which more than half of the tongue had been resected underwent reconstruction in which the cutaneous nerve of a free flap was anastomosed to the stump of the transected lingual nerve. Eight of the patients underwent reconstruc...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-199910000-00010

    authors: Kimata Y,Uchiyama K,Ebihara S,Kishimoto S,Asai M,Saikawa M,Ohyama W,Haneda T,Hayashi R,Onitsuka T,Nakatsuka T,Harii K

    更新日期:1999-10-01 00:00:00

  • Shaped versus Round Implants in Breast Reconstruction: A Multi-Institutional Comparison of Surgical and Patient-Reported Outcomes.

    abstract:BACKGROUND:Since the 2012 approval of shaped implants, their use in breast reconstruction has increased in the United States. However, large-scale comparisons of complications and patient-reported outcomes are lacking. The authors endeavored to compare surgical and patient-reported outcomes across implant types. METHO...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章,多中心研究

    doi:10.1097/PRS.0000000000003238

    authors: Khavanin N,Clemens MW,Pusic AL,Fine NA,Hamill JB,Kim HM,Qi J,Wilkins EG,Kim JYS

    更新日期:2017-05-01 00:00:00

  • Carpal tunnel syndrome in women undergoing reduction mammaplasty.

    abstract::Women with mammary hypertrophy who present for reduction mammaplasty have several well-described musculoskeletal complaints, but a high prevalence of carpal tunnel syndrome has not been reported. We identified 151 patients from a plastic surgery practice who underwent reduction mammaplasty from 1994 to 1996. To this g...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-200004040-00009

    authors: Pernia LR,Ronel DN,Leeper JD,Miller HL

    更新日期:2000-04-01 00:00:00

  • Complications after polyacrylamide hydrogel injection for facial soft-tissue augmentation in China: twenty-four cases and their surgical management.

    abstract:BACKGROUND:Polyacrylamide hydrogel has been used for soft-tissue augmentation for more than 10 years. Although it is considered a nontoxic, nonimmunogenic material, complications after polyacrylamide hydrogel injections during facial soft-tissue augmentation have been reported. METHODS:Between 2003 and 2009, 24 patien...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/PRS.0b013e3182589b7d

    authors: Shen H,Lv Y,Xu JH,Hong XY,Zeng BW,Xiao W,Zheng LJ

    更新日期:2012-08-01 00:00:00

  • Cost analysis of functional restoration surgery for extremity soft-tissue sarcoma.

    abstract:BACKGROUND:Limb-sparing surgery, consisting of wide-margin tumor resection and preoperative or postoperative radiotherapy/chemotherapy, has become the surgical treatment of choice for extremity sarcomas. However, adequate tumor resection can sometimes compromise crucial limb function, necessitating functional restorati...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/01.prs.0000187140.83705.cf

    authors: Nelson AA,Frassica FJ,Gordon TA,Deune EG

    更新日期:2006-01-01 00:00:00

  • APACHE II scoring for the prediction of immediate surgical complications in head and neck cancer patients.

    abstract:BACKGROUND:Aggressive surgical treatment of oral and oropharyngeal cancers may be compromised by significant surgical complications. Early identification of patients at risk for postoperative complications may assist in clinical decision-making. The objective of this study was to assess the value of the Acute Physiolog...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/01.prs.0000259038.36210.f8

    authors: Grant CA,Dempsey GA,Lowe D,Brown JS,Vaughan ED,Rogers SN

    更新日期:2007-05-01 00:00:00

  • Financial conflicts of interest in plastic surgery: background, potential for bias, disclosure, and transparency.

    abstract::Relationships between physicians and industry, whether it be pharmaceutical companies, medical device manufacturers, or purveyors of medical technology, contain both an element of potential for good and a potential for harm. Certainly, the potential for good is realized when the collaboration results in improved plast...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/PRS.0000000000000788

    authors: Luce EA

    更新日期:2015-04-01 00:00:00

  • Three- and four-dimensional arterial and venous anatomies of the thoracodorsal artery perforator flap.

    abstract:BACKGROUND:The vascular anatomy of the thoracodorsal artery perforator flap, which has not previously been elucidated, was examined using three- and four-dimensional computed tomographic angiography and venography. METHODS:Twenty-five thoracodorsal artery perforator flaps were harvested from fresh cadavers from the We...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/PRS.0b013e31816b1479

    authors: Schaverien M,Saint-Cyr M,Arbique G,Brown SA,Rohrich RJ

    更新日期:2008-05-01 00:00:00

  • Alternatives to subcutaneous mastectomy.

    abstract::It is our belief that subcutaneous mastectomy often leads to complications, with poor results. In addition, because a considerable amount of breast tissue may be left behind, its value as a prophylactic operation must be questioned. Two alternatives are presented. In our experience, these lead to fewer complications w...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-197908000-00014

    authors: Hoffman S,Simon BE,Kahn S

    更新日期:1979-08-01 00:00:00

  • Redefining the Ideal Buttocks: A Population Analysis.

    abstract:BACKGROUND:The buttocks are a key element of female beauty, with aesthetic gluteoplasty becoming one of the fastest growing plastic surgery procedures. However, there remains no clear standard for the ideal buttocks. The authors performed a population analysis of the characteristics of the ideal buttocks to guide surgi...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/PRS.0000000000002192

    authors: Wong WW,Motakef S,Lin Y,Gupta SC

    更新日期:2016-06-01 00:00:00

  • Migration studies and histology of injectable microspheres of different sizes in mice.

    abstract::Injectable dermal filler materials consist of either fluids, biological fragments, or suspensions of particles or microspheres. Particles and microspheres are said to "migrate," but migration can occur only when they are injected into blood vessels. To evaluate biocompatibility and transport, five nonresorbable polyme...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/01.prs.0000112764.22839.7a

    authors: Lemperle G,Morhenn VB,Pestonjamasp V,Gallo RL

    更新日期:2004-04-15 00:00:00

  • The surgical treatment of congenital webbing (pterygium) of the popliteal area.

    abstract::We describe a case of congenital popliteal pterygium in conjunction with other specific congenital malformations. This syndrome has been called the quadruple syndrome. The literature is reviewed. A rationale for more aggressive treatment of this deformity is discussed. One child who has undergone major multiple nerve ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-197509000-00020

    authors: Tuerk D,Edgerton MT

    更新日期:1975-09-01 00:00:00

  • Surgical Treatment and Visual Outcomes of Adult Orbital Roof Fractures.

    abstract:BACKGROUND:Fractures of the orbital roof require high-energy trauma and have been linked to high rates of neurologic and ocular complications. However, there is a paucity of literature exploring the association between injury, management, and visual prognosis. METHODS:The authors performed a 3-year retrospective revie...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/PRS.0000000000007436

    authors: Gebran SG,Lopez J,Wasicek PJ,Elegbede A,Rasko YM,Liang F,Nam AJ,Manson PN,Grant MP

    更新日期:2021-01-01 00:00:00

  • Evidence-based medicine: Unilateral cleft lip and nose repair.

    abstract:LEARNING OBJECTIVES:After reading this article, the participant should be able to: 1. Describe the anatomical malformations found in unilateral cleft lip deformity. 2. Discuss current methods of measuring the deformity and subsequent outcomes. 3. Discuss preoperative assessments, workup, and the use of early interventi...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章,评审

    doi:10.1097/PRS.0000000000000721

    authors: Greives MR,Camison L,Losee JE

    更新日期:2014-12-01 00:00:00

  • Anaphylactic-like reaction to methylene blue: case report and review of perioperative hypersensitivity reactions.

    abstract:BACKGROUND:Anaphylaxis and anaphylactic-like reactions in the perioperative period are a significant concern for anesthesiologists and surgeons. With an incidence as high as one in 3500 and a mortality rate of 3 to 6 percent, proper management of these reactions is essential. The available surgical literature provides ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/PRS.0b013e318254b2b8

    authors: Giladi AM,Kasten SJ

    更新日期:2012-07-01 00:00:00

  • Randomized comparison of the single-injection volar subcutaneous block and the two-injection dorsal block for digital anesthesia.

    abstract:BACKGROUND:Two commonly used methods of digital nerve block with local anesthetic are the two-injection dorsal technique and the single-injection volar subcutaneous technique. The authors compared these two digital block techniques with respect to local anesthetic injection pain and recipient preference of anesthetic t...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章,随机对照试验

    doi:10.1097/01.prs.0000237016.00941.96

    authors: Williams JG,Lalonde DH

    更新日期:2006-10-01 00:00:00

  • Propranolol-epinephrine interaction: a potential disaster.

    abstract::Presented here are six examples of potentially life-threatening propranolol-epinephrine interactions. The only report found that warns of a deleterious clinical interaction between propranolol and epinephrine appeared in 1980. With widespread use of propranolol for approved and unapproved conditions, the population at...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章

    doi:10.1097/00006534-198307000-00017

    authors: Foster CA,Aston SJ

    更新日期:1983-07-01 00:00:00