Localization and role of RAP55/LSM14 in HeLa cells: a new finding on the mitotic spindle assembly.

Abstract:

:The MAP family includes large proteins like MAP-1A, MAP-1B, MAP-1C, MAP-2, and MAP-4 and smaller components like tau and MAP-2C. This article focuses on the relevant aspects of RAP55/LSM14 position with emphasis on its role in mitotic spindle formation and stability. In this context, the localization of RNA associated Protein 55kDa (RAP55/LSM14) during mitosis was identified as a Mitotic Spindle Protein (MSP). We found a new location obtained by expressing GFP-tagged proteins in HeLa Cells during mitosis that has never been previously reported. We demonstrated also, for the first time, that the depletion of RAP55/LSM14 destabilizes spindle assembly, stops cells in mitosis and induces many other cell cytoskeletal disorders. Finally, by using an "in vitro" assay investigation, we found that RAP55/LSM14 binds directly the tubulin and that is implicated in the process of the mitotic spindle stabilization, which is a novel discovery in this field of research.

journal_name

Acta Biochim Pol

journal_title

Acta biochimica Polonica

authors

Mili D,Georgesse D,Kenani A

doi

10.18388/abp.2015_1107

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

613-9

issue

3

eissn

0001-527X

issn

1734-154X

pii

2015_1107

journal_volume

62

pub_type

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