Abstract:
:To estimate membrane potential in synaptosomes with the use of tetraphenylphosphonium (TPP+), an equation relating the amount of TPP+ accumulated in synaptosomes with membrane potential was derived from the following two assumptions. (1) TPP+ molecules were distributed into plasma membranes, mitochondria and cytosol of synaptosomes. (2) TPP+ achieves a Nernst equilibrium across both the synaptosomal and inner mitochondrial membranes. We propose three methods for calculation of membrane potential using this equation. The concentration of TPP+ was measured under various controlled conditions with an electrode selective for TPP+. The amount of TPP+ accumulated in synaptosomes was determined by measuring the difference between its initial concentration and the concentration after addition of synaptosomes, and membrane potential was estimated by the three methods. The resting potential of synaptosomes was estimated to be -75 to -90 mV by all of these methods. Membrane potentials under various controlled conditions were calculated, and the characteristics of the methods for estimation of membrane potential and those of membrane potential obtained by the methods are discussed.
journal_name
Chem Pharm Bull (Tokyo)journal_title
Chemical & pharmaceutical bulletinauthors
Aiuchi T,Matsunaga M,Nakaya K,Nakamura Ydoi
10.1248/cpb.37.3333subject
Has Abstractpub_date
1989-12-01 00:00:00pages
3333-7issue
12eissn
0009-2363issn
1347-5223journal_volume
37pub_type
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pub_type: 杂志文章
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journal_title:Chemical & pharmaceutical bulletin
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pub_type: 杂志文章
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journal_title:Chemical & pharmaceutical bulletin
pub_type: 杂志文章
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journal_title:Chemical & pharmaceutical bulletin
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journal_title:Chemical & pharmaceutical bulletin
pub_type: 杂志文章,评审
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journal_title:Chemical & pharmaceutical bulletin
pub_type: 杂志文章
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journal_title:Chemical & pharmaceutical bulletin
pub_type: 杂志文章
doi:10.1248/cpb.49.525
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journal_title:Chemical & pharmaceutical bulletin
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journal_title:Chemical & pharmaceutical bulletin
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journal_title:Chemical & pharmaceutical bulletin
pub_type: 杂志文章
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更新日期:2008-04-01 00:00:00
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journal_title:Chemical & pharmaceutical bulletin
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journal_title:Chemical & pharmaceutical bulletin
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journal_title:Chemical & pharmaceutical bulletin
pub_type: 杂志文章
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journal_title:Chemical & pharmaceutical bulletin
pub_type: 杂志文章
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journal_title:Chemical & pharmaceutical bulletin
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Chemical & pharmaceutical bulletin
pub_type: 杂志文章
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更新日期:2006-08-01 00:00:00
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journal_title:Chemical & pharmaceutical bulletin
pub_type: 杂志文章
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更新日期:2007-04-01 00:00:00
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journal_title:Chemical & pharmaceutical bulletin
pub_type: 杂志文章
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更新日期:2013-01-01 00:00:00
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journal_title:Chemical & pharmaceutical bulletin
pub_type: 杂志文章
doi:10.1248/cpb.38.1983
更新日期:1990-07-01 00:00:00
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journal_title:Chemical & pharmaceutical bulletin
pub_type: 杂志文章
doi:10.1248/cpb.53.100
更新日期:2005-01-01 00:00:00