Identification of Novel Genes Mediating Survival of Salmonella on Low-Moisture Foods via Transposon Sequencing Analysis.

Abstract:

:Salmonella enterica is the leading foodborne pathogen associated with outbreaks involving low-moisture foods (LMFs). However, the genes involved in Salmonella's long-term survival on LMFs remain poorly characterized. In this study, in-shell pistachios were inoculated with Tn5-based mutant libraries of S. Enteritidis P125109, S. Typhimurium 14028s, and S. Newport C4.2 at approximate 108 CFU/g and stored at 25°C. Transposon sequencing analysis (Tn-seq) was then employed to determine the relative abundance of each Tn5 insertion site immediately after inoculation (T0), after drying (T1), and at 120 days (T120). In S. Enteritidis, S. Typhimurium, and S. Newport mutant libraries, the relative abundance of 51, 80, and 101 Tn5 insertion sites, respectively, was significantly lower at T1 compared to T0, while in libraries of S. Enteritidis and S. Typhimurium the relative abundance of 42 and 68 Tn5 insertion sites, respectively, was significantly lower at T120 compared to T1. Tn5 insertion sites with reduced relative abundance in this competition assay were localized in DNA repair, lipopolysaccharide biosynthesis and stringent response genes. Twelve genes among those under strong negative selection in the competition assay were selected for further study. Whole gene deletion mutants in ten of these genes, sspA, barA, uvrB, damX, rfbD, uvrY, lrhA, yifE, rbsR, and ompR, were impaired for individual survival on pistachios. The findings highlight the value of combined mutagenesis and sequencing to identify novel genes important for the survival of Salmonella in low-moisture foods.

journal_name

Front Microbiol

authors

Jayeola V,McClelland M,Porwollik S,Chu W,Farber J,Kathariou S

doi

10.3389/fmicb.2020.00726

subject

Has Abstract

pub_date

2020-05-15 00:00:00

pages

726

issn

1664-302X

journal_volume

11

pub_type

杂志文章
  • Links between Soil Fungal Diversity and Plant and Soil Properties on the Loess Plateau.

    abstract::Previous studies have revealed inconsistent correlations between fungal diversity and plant/soil properties from local to global scales. Here, we investigated the internal relationships between soil fungal diversity and plant/soil properties on the Loess Plateau following vegetation restoration, using Illumina sequenc...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02198

    authors: Yang Y,Dou Y,Huang Y,An S

    更新日期:2017-11-07 00:00:00

  • The giant ciliate Zoothamnium niveum and its thiotrophic epibiont Candidatus Thiobios zoothamnicoli: a model system to study interspecies cooperation.

    abstract::Symbioses between chemoautotrophic sulfur-oxidizing (thiotrophic) bacteria and protists or animals are among the most diverse and prevalent in the ocean. They are extremely difficult to maintain in aquaria and no thiotrophic symbiosis involving an animal host has ever been successfully cultivated. In contrast, we have...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2014.00145

    authors: Bright M,Espada-Hinojosa S,Lagkouvardos I,Volland JM

    更新日期:2014-04-07 00:00:00

  • A modular method for the extraction of DNA and RNA, and the separation of DNA pools from diverse environmental sample types.

    abstract::A method for the extraction of nucleic acids from a wide range of environmental samples was developed. This method consists of several modules, which can be individually modified to maximize yields in extractions of DNA and RNA or separations of DNA pools. Modules were designed based on elaborate tests, in which permu...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00476

    authors: Lever MA,Torti A,Eickenbusch P,Michaud AB,Šantl-Temkiv T,Jørgensen BB

    更新日期:2015-05-19 00:00:00

  • High Diversity of Myocyanophage in Various Aquatic Environments Revealed by High-Throughput Sequencing of Major Capsid Protein Gene With a New Set of Primers.

    abstract::Myocyanophages, a group of viruses infecting cyanobacteria, are abundant and play important roles in elemental cycling. Here we investigated the particle-associated viral communities retained on 0.2 μm filters and in sediment samples (representing ancient cyanophage communities) from four ocean and three lake location...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00887

    authors: Hou W,Wang S,Briggs BR,Li G,Xie W,Dong H

    更新日期:2018-05-03 00:00:00

  • Temporally Variable Geographical Distance Effects Contribute to the Assembly of Root-Associated Fungal Communities.

    abstract::Root-associated fungi are key contributors to ecosystem functioning, however, the factors which determine community assembly are still relatively poorly understood. This study simultaneously quantified the roles of geographical distance, environmental heterogeneity and time in determining root-associated fungal commun...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00195

    authors: Barnes CJ,van der Gast CJ,Burns CA,McNamara NP,Bending GD

    更新日期:2016-02-25 00:00:00

  • Genomic and Secondary Metabolite Analyses of Streptomyces sp. 2AW Provide Insight into the Evolution of the Cycloheximide Pathway.

    abstract::The dearth of new antibiotics in the face of widespread antimicrobial resistance makes developing innovative strategies for discovering new antibiotics critical for the future management of infectious disease. Understanding the genetics and evolution of antibiotic producers will help guide the discovery and bioenginee...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00573

    authors: Stulberg ER,Lozano GL,Morin JB,Park H,Baraban EG,Mlot C,Heffelfinger C,Phillips GM,Rush JS,Phillips AJ,Broderick NA,Thomas MG,Stabb EV,Handelsman J

    更新日期:2016-05-03 00:00:00

  • Prediction of T4SS Effector Proteins for Anaplasma phagocytophilum Using OPT4e, A New Software Tool.

    abstract::Type IV secretion systems (T4SS) are used by a number of bacterial pathogens to attack the host cell. The complex protein structure of the T4SS is used to directly translocate effector proteins into host cells, often causing fatal diseases in humans and animals. Identification of effector proteins is the first step in...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01391

    authors: Esna Ashari Z,Brayton KA,Broschat SL

    更新日期:2019-06-21 00:00:00

  • Adaptation to Blue Light in Marine Synechococcus Requires MpeU, an Enzyme with Similarity to Phycoerythrobilin Lyase Isomerases.

    abstract::Marine Synechococcus has successfully adapted to environments with different light colors, which likely contributes to this genus being the second most abundant group of microorganisms worldwide. Populations of Synechococcus that grow in deep, blue ocean waters contain large amounts of the blue-light absorbing chromop...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00243

    authors: Mahmoud RM,Sanfilippo JE,Nguyen AA,Strnat JA,Partensky F,Garczarek L,Abo El Kassem N,Kehoe DM,Schluchter WM

    更新日期:2017-02-21 00:00:00

  • Meta-Analysis of Vaginal Microbiome Data Provides New Insights Into Preterm Birth.

    abstract::Preterm birth (PTB) is defined as the birth of an infant before 37 weeks of gestational age. It is the leading cause of perinatal morbidity and mortality worldwide. In this study, we present a comprehensive meta-analysis of vaginal microbiome in PTB. We integrated raw longitudinal 16S rRNA vaginal microbiome data from...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00476

    authors: Kosti I,Lyalina S,Pollard KS,Butte AJ,Sirota M

    更新日期:2020-04-08 00:00:00

  • Characterization of the Gut Microbiome Using 16S or Shotgun Metagenomics.

    abstract::The advent of next generation sequencing (NGS) has enabled investigations of the gut microbiome with unprecedented resolution and throughput. This has stimulated the development of sophisticated bioinformatics tools to analyze the massive amounts of data generated. Researchers therefore need a clear understanding of t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00459

    authors: Jovel J,Patterson J,Wang W,Hotte N,O'Keefe S,Mitchel T,Perry T,Kao D,Mason AL,Madsen KL,Wong GK

    更新日期:2016-04-20 00:00:00

  • Microbial Communities in Sediments of Lagos Lagoon, Nigeria: Elucidation of Community Structure and Potential Impacts of Contamination by Municipal and Industrial Wastes.

    abstract::Estuarine sediments are significant repositories of anthropogenic contaminants, and thus knowledge of the impacts of pollution upon microbial communities in these environments is important to understand potential effects on estuaries as a whole. The Lagos lagoon (Nigeria) is one of Africa's largest estuarine ecosystem...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01213

    authors: Obi CC,Adebusoye SA,Ugoji EO,Ilori MO,Amund OO,Hickey WJ

    更新日期:2016-08-05 00:00:00

  • A New Whole Genome Culture-Independent Diagnostic Test (WG-CIDT) for Rapid Detection of Salmonella in Lettuce.

    abstract::The rapid detection of foodborne microbial pathogens contaminating fresh fruits and vegetables during the intervening period between harvest and consumption could revolutionize microbial quality assurance of food usually consumed raw and those with a limited shelf life. We have developed a sensitive, shotgun whole gen...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00602

    authors: Ogunremi D,Dupras AA,Naushad S,Gao R,Duceppe MO,Omidi K,Márquez IG,Huang H,Goodridge L,Lévesque RC,Hasan NA,Dadlani M,Dixon B,Magierowski S,Masson L

    更新日期:2020-04-17 00:00:00

  • The MHC-II transactivator CIITA, a restriction factor against oncogenic HTLV-1 and HTLV-2 retroviruses: similarities and differences in the inhibition of Tax-1 and Tax-2 viral transactivators.

    abstract::The activation of CD4(+) T helper cells is strictly dependent on the presentation of antigenic peptides by MHC class II (MHC-II) molecules. MHC-II expression is primarily regulated at the transcriptional level by the AIR-1 gene product CIITA (class II transactivator). Thus, CIITA plays a pivotal role in the triggering...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2013.00234

    authors: Forlani G,Abdallah R,Accolla RS,Tosi G

    更新日期:2013-08-22 00:00:00

  • Development and Identification of a Novel Anti-HIV-1 Peptide Derived by Modification of the N-Terminal Domain of HIV-1 Integrase.

    abstract::The viral enzyme integrase (IN) is essential for the replication of human immunodeficiency virus type 1 (HIV-1) and represents an important target for the development of new antiretroviral drugs. In this study, we focused on the N-terminal domain (NTD), which is mainly involved into protein oligomerization process, fo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00845

    authors: Sala M,Spensiero A,Esposito F,Scala MC,Vernieri E,Bertamino A,Manfra M,Carotenuto A,Grieco P,Novellino E,Cadeddu M,Tramontano E,Schols D,Campiglia P,Gomez-Monterrey IM

    更新日期:2016-06-10 00:00:00

  • Correlation of Salmonella enterica and Listeria monocytogenes in Irrigation Water to Environmental Factors, Fecal Indicators, and Bacterial Communities.

    abstract::Outbreaks of foodborne illnesses linked to fresh fruits and vegetables have been key drivers behind a wide breadth of research aiming to fill data gaps in our understanding of the total ecology of agricultural water sources such as ponds and wells and the relationship of this ecology to foodborne pathogens such as Sal...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.557289

    authors: Gu G,Strawn LK,Ottesen AR,Ramachandran P,Reed EA,Zheng J,Boyer RR,Rideout SL

    更新日期:2021-01-08 00:00:00

  • Total Arsenic, pH, and Sulfate Are the Main Environmental Factors Affecting the Microbial Ecology of the Water and Sediments in Hulun Lake, China.

    abstract::Bacteria have the metabolic potential to produce a diverse array of secondary metabolites, which have important roles in biogeochemical cycling processes. However, for Hulun Lake and the rivers that enter into it, the bacterial community structures and their effects have not previously been widely studied, limiting ou...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.548607

    authors: Shang Y,Wu X,Wei Q,Dou H,Wang X,Chen J,Zhang H,Ma S,Zhang H

    更新日期:2020-09-24 00:00:00

  • Swine-Derived Probiotic Lactobacillus plantarum Inhibits Growth and Adhesion of Enterotoxigenic Escherichia coli and Mediates Host Defense.

    abstract::Weaning stress renders piglets susceptible to pathogen infection, which leads to post-weaning diarrhea, a severe condition characterized by heavy diarrhea and mortality in piglets. Enterotoxigenic Escherichia coli (ETEC) is one of typical strains associated with post-weaning diarrhea. Thus, prevention and inhibition o...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01364

    authors: Wang J,Zeng Y,Wang S,Liu H,Zhang D,Zhang W,Wang Y,Ji H

    更新日期:2018-06-26 00:00:00

  • Molecular Targets for Coevolutionary Interactions Between Pacific Oyster Larvae and Their Sympatric Vibrios.

    abstract::Bacteria of the Vibrio genus are the most predominant infectious agents threatening marine wildlife and aquaculture. Due to the large genetic diversity of these pathogens, the molecular determinants of Vibrio virulence are only poorly understood. Furthermore, studies tend to ignore co-evolutionary interactions between...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02067

    authors: Wegner KM,Piel D,Bruto M,John U,Mao Z,Alunno-Bruscia M,Petton B,Le Roux F

    更新日期:2019-09-06 00:00:00

  • Antibacterial Effect of Copaifera duckei Dwyer Oleoresin and Its Main Diterpenes against Oral Pathogens and Their Cytotoxic Effect.

    abstract::This study evaluates the antibacterial activity of the Copaifera duckei Dwyer oleoresin and two isolated compounds [eperu-8(20)-15,18-dioic acid and polyalthic acid] against bacteria involved in primary endodontic infections and dental caries and assesses the cytotoxic effect of these substances against a normal cell ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00201

    authors: Abrão F,Alves JA,Andrade G,de Oliveira PF,Ambrósio SR,Veneziani RCS,Tavares DC,Bastos JK,Martins CHG

    更新日期:2018-02-21 00:00:00

  • A Genetic and Chemical Perspective on Symbiotic Recruitment of Cyanobacteria of the Genus Nostoc into the Host Plant Blasia pusilla L.

    abstract::Liverwort Blasia pusilla L. recruits soil nitrogen-fixing cyanobacteria of genus Nostoc as symbiotic partners. In this work we compared Nostoc community composition inside the plants and in the soil around them from two distant locations in Northern Norway. STRR fingerprinting and 16S rDNA phylogeny reconstruction sho...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01693

    authors: Liaimer A,Jensen JB,Dittmann E

    更新日期:2016-11-01 00:00:00

  • Characterization of Vibrio cholerae's Extracellular Nuclease Xds.

    abstract::The Gram-negative bacterium Vibrio cholerae encodes two nucleases, Dns and Xds, which play a major role during the human pathogen's lifecycle. Dns and Xds control three-dimensional biofilm formation and bacterial detachment from biofilms via degradation of extracellular DNA and thus contribute to the environmental, in...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02057

    authors: Pressler K,Mitterer F,Vorkapic D,Reidl J,Oberer M,Schild S

    更新日期:2019-09-10 00:00:00

  • Pyrosequencing detects human and animal pathogenic taxa in the grapevine endosphere.

    abstract::Generally, plants are not considered as hosts for human and animal pathogens (HAP). The recent produce-associated outbreaks of food-borne diseases have drawn attention toward significant deficiencies in our understanding of the ecology of HAP, and their potential for interkingdom transfer. To examine the association o...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00327

    authors: Yousaf S,Bulgari D,Bergna A,Pancher M,Quaglino F,Casati P,Campisano A

    更新日期:2014-07-08 00:00:00

  • Identifying reference genes with stable expression from high throughput sequence data.

    abstract::Genes that are constitutively expressed across multiple environmental stimuli are crucial to quantifying differentially expressed genes, particularly when employing quantitative reverse transcriptase polymerase chain reaction (RT-qPCR) assays. However, the identification of these potential reference genes in non-model...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00385

    authors: Alexander H,Jenkins BD,Rynearson TA,Saito MA,Mercier ML,Dyhrman ST

    更新日期:2012-11-09 00:00:00

  • Heterologous Production of a Novel Cyclic Peptide Compound, KK-1, in Aspergillus oryzae.

    abstract::A novel cyclic peptide compound, KK-1, was originally isolated from the plant-pathogenic fungus Curvularia clavata. It consists of 10 amino acid residues, including five N-methylated amino acid residues, and has potent antifungal activity. Recently, the genome-sequencing analysis of C. clavata was completed, and the b...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00690

    authors: Yoshimi A,Yamaguchi S,Fujioka T,Kawai K,Gomi K,Machida M,Abe K

    更新日期:2018-04-09 00:00:00

  • Long-term impact of farm management and crops on soil microorganisms assessed by combined DGGE and PLFA analyses.

    abstract::In the present study, long-term organic and conventional managements were compared at the experimental field of Monsampolo del Tronto (Marche region, Italy) with the aim of investigating soil chemical fertility and microbial community structure. A polyphasic approach, combining soil fertility indicators with microbiol...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00644

    authors: Stagnari F,Perpetuini G,Tofalo R,Campanelli G,Leteo F,Della Vella U,Schirone M,Suzzi G,Pisante M

    更新日期:2014-12-10 00:00:00

  • Chitosan, a Biopolymer With Triple Action on Postharvest Decay of Fruit and Vegetables: Eliciting, Antimicrobial and Film-Forming Properties.

    abstract::Chitosan is a natural biopolymer from crab shells that is known for its biocompatibility, biodegradability, and bioactivity. In human medicine, chitosan is used as a stabilizer for active ingredients in tablets, and is popular in slimming diets. Due to its low toxicity, it was the first basic substance approved by the...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.02745

    authors: Romanazzi G,Feliziani E,Sivakumar D

    更新日期:2018-12-04 00:00:00

  • Bioactive Molecule from Streptomyces sp. Mitigates MDR Klebsiella pneumoniae in Zebrafish Infection Model.

    abstract::The emergence and spread of multi-drug resistant (MDR) especially carbapenem-resistant Klebsiella pneumoniae is a major emerging threat to public health, leading to excess in mortality rate as high as 50-86%. MDR K. pneumoniae manifests all broad mechanisms of drug resistance, hence development of new drugs to treat M...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00614

    authors: Cheepurupalli L,Raman T,Rathore SS,Ramakrishnan J

    更新日期:2017-04-12 00:00:00

  • Temporal changes in the diazotrophic bacterial communities associated with Caribbean sponges Ircinia stroblina and Mycale laxissima.

    abstract::Sponges that harbor microalgal or, cyanobacterial symbionts may benefit from photosynthetically derived carbohydrates, which are rich in carbon but devoid of nitrogen, and may therefore encounter nitrogen limitation. Diazotrophic communities associated with two Caribbean sponges, Ircinia strobilina and Mycale laxissim...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00561

    authors: Zhang F,Vicente J,Hill RT

    更新日期:2014-10-28 00:00:00

  • Different Effects of Premature Infant Formula and Breast Milk on Intestinal Microecological Development in Premature Infants.

    abstract::Intestinal microecology has been shown to participate in the pathogenesis of many diseases through different pathways, and the intestinal microecology of premature infants is significantly different from full-term infants. Intestinal microecology in premature infants is affected by various factors such as gestational ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.03020

    authors: Chen C,Yin Q,Wu H,Cheng L,Kwon JI,Jin J,Han T,Che H

    更新日期:2020-01-17 00:00:00

  • Global Emergence and Dissemination of Enterococci as Nosocomial Pathogens: Attack of the Clones?

    abstract::Enterococci are Gram-positive bacteria that are found in plants, soil and as commensals of the gastrointestinal tract of humans, mammals, and insects. Despite their commensal nature, they have also become globally important nosocomial pathogens. Within the genus Enterococcus, Enterococcus faecium, and Enterococcus fae...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.00788

    authors: Guzman Prieto AM,van Schaik W,Rogers MR,Coque TM,Baquero F,Corander J,Willems RJ

    更新日期:2016-05-26 00:00:00