Effects of local administration of allogenic adipose tissue-derived mesenchymal stem cells on functional recovery in experimental traumatic brain injury.

Abstract:

OBJECTIVE:Traumatic brain injury (TBI) is the leading cause of mortality and morbidity in paediatric patients after the first year of life. The aim of this study was to evaluate effects of locally administered allogeneic mesenchymal stem cells (MSC), in the acute period after a TBI. METHODOLOGY:MSC were isolated from peritoneal fat of healthy rats, expanded in vitro and labelled with the green fluorescent protein. Rats were placed in one of three experimental groups: (1) CONTROL: TBI, (2) IP-CONTROL: TBI + local saline and (3) IP-Treat: TBI + 2 × 10(5) MSC 24 hours after receiving a moderate, unilateral, controlled cortical impact. Motor and cognitive behavioural tests were performed to evaluate functional recovery. Histological examination and immunohistochemistry were used to identify cell distribution. MAIN RESULTS:Improved performance was found on motor tests in the MSC-treated group compared to control groups. MSC were found in the perilesional area and their number decreased with time after transplantation. MSC treatment increased the cell density in the hippocampus (CA3 pyramidal cells and granule cells in the dentate gyrus) and enhanced neurogenesis in this area. CONCLUSION:MSC cell therapy resulted in better recovery of motor function compared with the control group. This cellular therapy might be considered for patients suffering from TBI.

journal_name

Brain Inj

journal_title

Brain injury

authors

Mastro-Martínez I,Pérez-Suárez E,Melen G,González-Murillo Á,Casco F,Lozano-Carbonero N,Gutiérrez-Fernández M,Díez-Tejedor E,Casado-Flores J,Ramírez-Orellana M,Serrano-González A

doi

10.3109/02699052.2015.1053525

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

1497-510

issue

12

eissn

0269-9052

issn

1362-301X

journal_volume

29

pub_type

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