Abstract：
:Cancer cell lines have been used widely in cancer biology, and as biological or functional cell systems in many biomedical research fields. These cells are usually defective for many normal activities or functions due to significant genetic and epigenetic changes. Normal primary cell yields and viability from any original tissue specimens are usually relatively low or highly variable. These normal cells cease after a few passages or population doublings due to very limited proliferative capacity. Animal models (ferret, mouse, etc.) are often used to study virus-host interaction. However, viruses usually need to be adapted to the animals by several passages due to tropism restrictions including viral receptors and intracellular restrictions. Here we summarize applications of conditionally reprogrammed cells (CRCs), long-term cultures of normal airway epithelial cells from human nose to lung generated by conditional cell reprogramming (CR) technology, as an ex vivo model in studies of emerging viruses. CR allows to robustly propagate cells from non-invasive or minimally invasive specimens, for example, nasal or endobronchial brushing. This process is rapid (2 days) and conditional. The CRCs maintain their differentiation potential and lineage functions, and have been used for studies of adenovirus, rhinovirus, respiratory syncytial virus, influenza viruses, parvovirus, and SARS-CoV. The CRCs can be easily used for air-liquid interface (ALI) polarized 3D cultures, and these coupled CRC/ALI cultures mimic physiological conditions and are suitable for studies of viral entry including receptor binding and internalization, innate immune responses, viral replications, and drug discovery as an ex vivo model for emerging viruses.
journal_name
Virol Sinjournal_title
Virologica Sinicaauthors
Liu X,Wu Y,Rong Ldoi
10.1007/s12250-020-00244-zsubject
Has Abstractpub_date
2020-06-01 00:00:00pages
280-289issue
3eissn
1674-0769issn
1995-820Xpii
10.1007/s12250-020-00244-zjournal_volume
35pub_type
杂志文章,评审abstract：:HIV-indeterminate Western blotting (WB) results are typically obtained in WB confirmatory assays, and the number of indeterminate samples may increase with the detection of HIV infections, which will present considerable challenges for the management of HIV/AIDS. Nucleic acid detection has been used as a laboratory te...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-019-00130-3
更新日期：2019-08-01 00:00:00
abstract：:Respiratory syncytial virus (RSV) is the key underlying cause of acute lower respiratory tract infection in infants; however, no licensed vaccine against RSV infection is currently available. This study was undertaken to assess the preventive effect of vaccine on RSV infection. In this metaanalysis, 1,792 published ra...
journal_title：Virologica Sinica
pub_type： 杂志文章,meta分析
doi：10.1007/s12250-015-3630-3
更新日期：2015-10-01 00:00:00
abstract：:Bat SARS-like coronavirus (SL-CoV) has a genome organization almost identical to that of SARS-CoV, but the N-terminus of the Spike (S) proteins, which interacts with host receptor and is a major target of neutralizing antibodies against CoVs, of the two viruses has only 63-64% sequence identity. Although there have be...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-013-3292-y
更新日期：2013-04-01 00:00:00
abstract：:Isolates of Newcastle disease virus (NDV) from deceased wild and domestic pigeons in Kazakhstan were obtained from the Almaty region during 2005 and were genotypically analyzed by using reverse transcription polymerase chain reaction (RT-PCR) with primers specific to the viral fusion (F) protein gene. Part of the ampl...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-012-3234-0
更新日期：2012-04-01 00:00:00
abstract：:Ebola virus (EBOV) causes severe hemorrhagic fever in humans and non-human primates with high rates of fatality. Glycoprotein (GP) is the only envelope protein of EBOV, which may play a critical role in virus attachment and entry as well as stimulating host protective immune responses. However, the lack of expression ...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-016-3845-y
更新日期：2016-12-01 00:00:00
abstract：:Neutralizing antibodies are considered to be an important protective parameter used in HIV-1 vaccine evaluation. However, the exact role that neutralizing antibodies plays in controlling the disease progression of HIV-1 infected peoples is still undetermined. In this paper, we compared the protective function of the n...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-012-3239-8
更新日期：2012-06-01 00:00:00
abstract：:The virus receptors are key for the viral infection of host cells. Identification of the virus receptors is still challenging at present. Our previous study has shown that human virus receptor proteins have some unique features including high N-glycosylation level, high number of interaction partners and high expressi...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-020-00259-6
更新日期：2020-07-28 00:00:00
abstract：:Omsk hemorrhagic fever virus (OHFV) is a tick-borne flavivirus classified as a biosafety level-4 (BSL4) pathogen. Studies of OHFV are restricted to be conducted within BSL4 laboratories. Currently, no commercial vaccines or antiviral drugs are available against OHFV infection. In this study, we recovered a replication...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-019-00109-0
更新日期：2019-08-01 00:00:00
abstract：:Porcine respiratory disease complex (PRDC) is a serious health problem that mainly affects growing and finishing pigs. PRDC is caused by a combination of viral and bacterial agents, such as porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza virus (SIV), Mycoplasma hyopneumoniae (Myh), Actinob...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-014-3471-5
更新日期：2014-08-01 00:00:00
abstract：:Exosomes are cell-derived vesicles that are secreted by many eukaryotic cells. It has recently attracted attention as vehicles of intercellular communication. Virus-infected cells release exosomes, which contain viral proteins, RNA, and pathogenic molecules. However, the role of exosomes in virus infection process rem...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-019-00087-3
更新日期：2019-02-01 00:00:00
abstract：:Oncolytic alphavirus M1 has been shown to selectively target and kill cancer cells, but cytopathic morphologies induced by M1 virus and the life cycle of the M1 strain in cancer cells remain unclear. Here, we study the key stages of M1 virus infection and replication in the M1 virus-sensitive HepG2 liver cancer cell l...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-020-00339-7
更新日期：2021-01-22 00:00:00
abstract：:Shiga toxin B-subunit (STxB) from Shigella dysenteriae targets in vivo antigen to cancer cells, dendritic cells (DC) and B cells, which preferentially express the globotriaosylceramide (Gb3) receptor. This pivotal role has encouraged scientists to investigate fusing STxB with other clinical antigens. Due to the challe...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-013-3286-9
更新日期：2013-02-01 00:00:00
abstract：:Our previous work has shown that Saffold virus (SAFV) induced several rodent and primate cell lines to undergo apoptosis (Xu et al. in Emerg Microb Infect 3:1-8, 2014), but the essential viral proteins of SAFV involved in apoptotic activity lack study. In this study, we individually transfected the viral proteins of S...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-019-00116-1
更新日期：2019-06-01 00:00:00
abstract：:Effective oral infection is set off by interaction of a group of conserved per os infectivity factors (PIFs) with larval midgut columnar epithelial cells. We constructed pseudotyped viruses by substituting pif1, pif2 or pif3 genes of Helicoverpa armigera nucleopolyhedrovirus (HearNPV) with their homologs from Mamestra...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-018-0014-5
更新日期：2018-04-01 00:00:00
abstract：:Avian infectious bronchitis virus (IBV) is a Gammacoronavirus in the family Coronaviridae and causes highly contagious respiratory disease in chickens. Innate immunity plays significant roles in host defense against IBV. Here, we explored the interaction between IBV and the host innate immune system. Severe histopatho...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-015-3696-y
更新日期：2016-02-01 00:00:00
abstract：:The epidemiology of HIV-1 varies in different areas of the world, and it is possible that this complexity may leave unique footprints in the viral genome. Thus, we attempted to find significant patterns in global HIV-1 genome sequences. By applying the rule inference algorithm RIPPER (Repeated Incremental Pruning to P...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-013-3348-z
更新日期：2013-08-01 00:00:00
abstract：:The papain-like protease (PL(pro)) of Middle-East respiratory syndrome coronavirus (MERS-CoV) has proteolytic, deubiquitinating, and deISGylating activities. The latter two are involved in the suppression of the antiviral innate immune response of the host cell. To contribute to an understanding of this process, we pr...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-016-3742-4
更新日期：2016-08-01 00:00:00
abstract：:Ebola virus (EBOV) and Marburg virus (MARV) are causative agents of severe hemorrhagic fever with high mortality rates in humans and non-human primates and there is currently no licensed vaccine or therapeutics. To date, there is no specific laboratory diagnostic test in China, while there is a national need to provid...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-012-3252-y
更新日期：2012-10-01 00:00:00
abstract：:The recent emergence and rapid global spread of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pose an unprecedented medical and socioeconomic crisis, and the disease caused by it, Coronavirus disease 2019 (COVID-19), was declared a pandemic by the World Health Organization (WHO) on March 11, 2020. C...
journal_title：Virologica Sinica
pub_type： 杂志文章,评审
doi：10.1007/s12250-020-00298-z
更新日期：2020-12-01 00:00:00
abstract：:Human cytomegalovirus (HCMV), a herpesvirus, is an important human pathogen that causes asymptomatic infections in healthy or immunocompetent individuals but can lead to severe and potentially life-threatening complications in immune-immature individuals such as neonates or immune-compromised patients such as organ-tr...
journal_title：Virologica Sinica
pub_type： 杂志文章,评审
doi：10.1007/s12250-017-4055-y
更新日期：2017-10-01 00:00:00
abstract：:An exogenous avian leukosis virus (ALV) strain SDAU09C1 was isolated in DF-1 cells from one of 240 imported 1-day-old white meat-type grand parent breeder chicks. Inoculation of SDAU09C1 in ALV-free chickens induced antibody reactions specific to subgroup A or B. But gp85 amino acid sequence comparisons indicated that...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-010-3095-3
更新日期：2010-04-01 00:00:00
abstract：:An emerging mosquito-borne arbovirus named Zika virus (ZIKV), of the family Flaviviridae and genus Flavivirus, is becoming a global health threat. ZIKV infection was long neglected due to its sporadic nature and mild symptoms. However, recently, with its rapid spread from Asia to the Americas, affecting more than 30 c...
journal_title：Virologica Sinica
pub_type： 杂志文章,评审
doi：10.1007/s12250-016-3780-y
更新日期：2016-04-01 00:00:00
abstract：:Histone deacetylase (HDAC) inhibitors show clinical promise for the treatment of cancers, including hepatocellular carcinoma (HCC). In this study, we investigated the effect of HDAC inhibitor treatment on hepatitis C virus (HCV) replication in Huh7 human liver cells and in a mouse model of HCV infection. Viral replica...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-018-0057-7
更新日期：2018-10-01 00:00:00
abstract：:The protein HTRP (human transcription regulator protein) is encoded by the differential gene htrp and induced by Herpes simplex virus type 1 (HSV-1) infection in KMB-17 cells. HTRP was found to interact with SAP30 (mSin3A Association Protein), one of the components of co-repressor complex mSin3A, which is part of the ...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-010-3147-8
更新日期：2010-12-01 00:00:00
abstract：:Newcastle disease virus (NDV) and H9N2 subtype Avian influenza virus (AIV) are two notorious avian respiratory pathogens that cause great losses in the poultry industry. Current inactivated commercial vaccines against NDV and AIV have the disadvantages of inadequate mucosal responses, while an attenuated live vaccine ...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-020-00199-1
更新日期：2020-08-01 00:00:00
abstract：:In this paper, in vitro anti-influenza virus activities of sulfated polysaccharide fractions from Gracilaria lemaneiformis were investigated. Cytotoxicities and antiviral activities of Gracilaria lemaneiformis polysaccharides (PGL), Gracilaria lemaneiformis polysaccharide fraction-1 (GL-1), Gracilaria lemaneiformis po...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-010-3137-x
更新日期：2010-10-01 00:00:00
abstract：:Circular RNAs (circRNAs) belong to a class of non-coding RNAs with diverse biological functions. However, little is known about their roles in case of pseudorabies virus (PrV) infection. Here, we analyzed the expression profile of host circRNAs from a virulent PrV type II strain DX (PrV-DX) infected and an attenuated ...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-020-00255-w
更新日期：2020-07-02 00:00:00
abstract：:Similar to Hepatitis C virus (HCV) infection in humans, HCVcc infection can also result in persistent and chronic infection. The core protein is a variable protein and exists in several sizes. Some sizes of core proteins have been reported to be related to chronic HCV infection. To study the possible role of the core ...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-013-3296-7
更新日期：2013-06-01 00:00:00
abstract：:Hepatitis E virus (HEV) is the prototype of the family Hepeviridae and the causative agent of common acute viral hepatitis. Genetically diverse HEV-related viruses have been detected in a variety of mammals and some of them may have zoonotic potential. In this study, we tested 278 specimens collected from seven wild s...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-018-0011-8
更新日期：2018-02-01 00:00:00
abstract：:Southern rice black-streaked dwarf virus (SRBSDV) is a novel Fijivirus prevalent in rice in southern and central China, and northern Vietnam. Its genome has 10 segments of double-stranded RNA named S1 to S10 according to their size. An isolate of SRBSDV, JNi4, was obtained from naturally infected maize plants from Ji'...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-011-3170-9
更新日期：2011-02-01 00:00:00
