Abstract:
:The aim of study was to evaluate the morphological and immunohystochemical characteristics of aspirated thrombi in patients with ST-elevation myocardial infarction undergoing precutaneous coronary intervention to find the possible platelet activation and inflammatory cell involvement in coronary thrombi. MATERIALS AND METHODS:Thrombi collected from 67 patients with ST-elevation myocardial infarction were studied. Macroscopic and immunohystochemical analyses were performed to reveal the cellular components of thrombi. The peroxidase - antiperoxidase immunohystochemical method was applied with monoclonal antibodies to CD4+, CD8+, CD15+, CD63+ and CD105+ using diaminobenzidine agent as a visualization agent to investigate the potential involvement of different cellular subpopulations. RESULTS:The macroscopic examination revealed friable thrombi with apparent laminations (lines of Zahn). Microscopic analysis of thrombi revealed a loose meshwork of fibrin fibers with compact accumulation of activated platelets. The platelets were surrounded by neutrophilic granulocytes.The immunohystochemical analysis confirmed that cell aggregates are consisting of CD 63+ activated platelets and leucocytes. The granulocytes also tested positive for CD15 antigen. The immunohystochemical analysis revealed the presence of CD8+ cytotoxic T-lymphocytes and CD4+ helper T-lymphocytes. No expression of iNOS, CD105 and VEGF was found in the analyzed thrombi. CONCLUSIONS:The hystopathological evaluation of thrombi in patients with acute ST-elevation myocardial infarction revealed the presence of activated CD63+ platelets and CD15+ neutrophilic granulocytes. Activated platelets and neutrophils may play a role in thromboinflammatory activation course leading to destabilization of atherosclerotic plaque and development of acute thrombosis in patients with ST-elevation acute myocardial infarction. :Цель исследования: оценить морфологические и иммуногистохимические характеристики аспирированных тромбов у пациентов с инфарктом миокарда с подъемом ST сегмента, которым провели чрескожное коронарное вмешательство. Материалы и методы. Исследовали тромбы, полученные от 67 пациентов с инфарктом миокарда с подъемом ST сегмента. Проводили макроскопический и иммуногистохимический анализы для определения клеточных компонентов тромбов. Использовали иммуногистохимический метод пероксидаза - антипероксидазы с помощью моноклональных антител к CD4, CD8, CD15, CD63 и CD105 с использованием диаминобензидина в качестве визуализирующего агента для исследования потенциального вовлечения различных клеточных субпопуляций. Результаты. Макроскопический анализ выявил рыхлые тромбы с видимыми слоями (lines of Zahn), которые при микроскопическом анализе выявлялись в виде рыхлой сетчатой структуры волокон фибрина с компактными скоплениями активированных тромбоцитов. Тромбоциты были окружены нейтрофильными гранулоцитами. Иммуногистохимический анализ подтвердил, что сгусток содержит CD63 позитивные активированные тромбоциты и лейкоциты. Гранулоциты были положительны к CD15-антигену. Иммуногистохимический анализ выявил также наличие CD8+ цитотоксических Т-лимфоцитов и CD4+ T-хелперных лимфоцитов. В анализированных тромбах не выявлено экспрессии iNOS, CD105 и сосудистого эндотелиального фактора роста. Заключение. Гистопатологический анализ тромбов у пациентов с острым инфарктом миокарда с подъемом ST сегмента выявил возможное наличие активированных CD63+-тромбоцитов и CD15+-нейтрофильных гранулоцитов. Активированные тромбоциты и нейтрофилы могут играть важную патогенетическую роль в тромбовоспалительном процессе, приводящем к дестабилизации атеросклеротической бляшки и развитию острого тромбоза при остром инфаркте миокарда с подъемом ST сегмента.
journal_name
Ter Arkhjournal_title
Terapevticheskii arkhivauthors
Sisakian HS,Mkhitaryan AG,Sahakyan VG,Kocharyan AS,Asatryan BAdoi
10.26442/00403660.2019.09.000199subject
Has Abstractpub_date
2019-09-15 00:00:00pages
21-25issue
9eissn
0040-3660issn
2309-5342journal_volume
91pub_type
杂志文章abstract:AIM:To study coronary-myocardial reserve in patients with a programmed pacemaker. MATERIALS AND METHODS:Stress-echocardiography was performed in 64 patients with coronary heart disease (CHD). The stress was induced by a gradual increase in the frequency of electrostimulation (ES) by 10 imp/min from initial 90 imp/min ...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::As many as 30 patients suffering from systemic lupus erythematosus (SLE) with the clinical signs of central nervous system derangement were examined. The mean age of the patients was 31.1 years. Using EIA, antibodies against cardiolipin (a-CL) were detected in 21 patients (70%). A-CL were revealed in all the patients ...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract::The relevance of investigating carbohydrate metabolism (CM) in patients with acromegaly and Itsenko-Cushing disease is attributable to frequent glucose metabolic disturbances, on the one hand, and to difficulties in choosing sugar-lowering therapy in these categories of patients, on the other. The efficiency of hyperg...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:10.17116/terarkh2015871098-104
更新日期:2015-01-01 00:00:00
abstract::The data obtained in a prospective study made it possible, using Markov's analysis, to assess different forms of coronary heart disease (CHD) of long duration and to elaborate an unsophisticated scale for estimating individual risk of death from that disease. The Markov's analysis demonstrated a high enough probabilit...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::Loop diuretics take the lead in the treatment of patients with symptomatic heart failure. Torasemide is the most effective and safe loop diuretic with the optimal pharmacokinetic profile, the additional properties associated with simultaneous blockade of the renin-angiotensin-aldosterone system and with the antiadrene...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章,评审
doi:10.17116/terarkh2017899115-119
更新日期:2017-01-01 00:00:00
abstract::A study of 186 coronary patients with rhythm disturbance has shown that early incorporation of the elements of physical rehabilitation in combined treatment improves functioning of all the links of the blood circulation system, helps to raise cardiac rhythm stability and reduces the period of patients' stay in hospita...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
abstract::Takayasu arteritis (TA) is a systemic vasculitis with predominatly lesions of aorta and its large branches. In some cases pulmonary arteries (PA) are involved in the pathological inflammatory process and lead to the formation of pulmonary hypertension and significantly worse the prognosis. Timely development of lesion...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:10.26442/00403660.2020.05.000623
更新日期:2020-06-05 00:00:00
abstract::Structural hemoglobinopathy due to carriage of Hb Camden was diagnosed in a male patient and his mother. Anomalous Hb was detected after a detailed analysis of the clinical evidence, blood smears microscopy, marrow examination, data obtained by a complex of methods to identify unstable Hb. It was defined as Hb Camden ...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract:AIM:To prove the value of summary data from the State Statistics Commission to characterize of risk of death from circulatory system diseases (CSD). MATERIAL AND METHODS:The official data of the State Statistics Commission of the Azerbaijan Republic, which are available at its website (www.stat.gov.az), were analyzed ...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:10.17116/terarkh201688352-55
更新日期:2016-01-01 00:00:00
abstract::The efficacy and tolerability of high-dose neurotrophic drug cerebrolysin was studied in 20 patients with vascular dementia diagnosed by criteria NINDS-AIREN and DSM-IV. The drug was well tolerated, affected positively cognitive function, especially neurodynamic processes. EEG featured after treatment intensification ...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract:AIM:To study the results of mobilizing and collecting autologous hematopoietic stem cells (HSC) in patients with multiple myeloma (MM) receiving bortezomib as part of induction therapy regimens. MATERIALS AND METHODS:In June 2001 to April 2010, the Department of Bone Marrow Transplantation, Hematology Research Center,...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:2012-01-01 00:00:00
abstract::The authors studied the activity of cholesterol esters transport (CET), concentrations of apolipoprotein E in the blood and high density lipoproteins (HDLP) in parallel with other parameters of lipoprotein metabolism in 79 males and 62 females. 122 of them had ischemic heart disease (IHD) and primary hyperlipoproteine...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::A total of 160 persons including 50 patients with duodenal ulcer (DU) and 38 with gastric ulcer (GU) were examined for antibodies to the parietal cells of the stomach (PCA) and cell cellular immunity responses to autologous antigen from the gastric mucosa. It was shown that both PCA of the stomach and cell immunity re...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract:AIM:To specify causes of discontinuation of the disease-modifying antirheumatic drugs (DMAD) used in rheumatoid arthritis (RA) by the results of special questionnaire survey. MATERIAL AND METHODS:The study included 298 RA patients treated in the Institute of Rheumatology (34 males and 264 females aged 18-82, mean age ...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::The paper gives an update on the pathogenetic role of viral infection and immune mechanisms in the development of cardiac dilatation at the cellular, ultrastructural, and molecular levels. Particular attention is given to the discussion of the possible role of herpesvirus infection in the mechanisms of cardiomyocyte d...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章,评审
doi:10.17116/terarkh2017891179-83
更新日期:2017-01-01 00:00:00
abstract::A study was made of adrenal function in 2 groups of patients with chronic renal insufficiency on programmed hemodialytic therapy. Insufficiency of gluco- and mineralo-corticoid function of the adrenal glands and hyperfunction of their medulla developed in patients with normal BP and controlled hypertension. In patient...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract:AIM:To analyse clinical implications of chromosome 8 trisomy in Ph-negative cells of the bone marrow in patients with chronic myeloid leukemia (CML) treated with inhibitors of tyrosinkinases (ITK). MATERIAL AND METHODS:A total of 386 patients with CML (chronic phase--288, acceleration phase--77) received imatinib (400...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
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journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
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journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract::Short-term results of rehabilitation of 1046 CHD patients at a cardiological sanatorium in mountains at average altitude (1600 m above the sea level) were studied. Large focal myocardial infarction (MI) was found in 738 of them, small focal MI in 249, and acute focal myocardial dystrophy of ischemic genesis in 59. Of ...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
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journal_title:Terapevticheskii arkhiv
pub_type: 临床试验,杂志文章
doi:
更新日期:2004-01-01 00:00:00
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journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
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journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:10.26442/terarkh201890915-26
更新日期:2018-09-20 00:00:00
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journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
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journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:10.26442/00403660.2019.04.000209
更新日期:2019-05-16 00:00:00
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journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
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journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章,meta分析
doi:
更新日期:2006-01-01 00:00:00
abstract:AIM:to investigate time course of changes in the adrenoreactivity (AR) of erythrocyte membranes (EM) after radiofrequency ablation of the synaptic nerves of the renal arteries (RA) in patients with resistant hypertension (RH) and to assess whether this indicator can be used for the early evaluation of the efficiency of...
journal_title:Terapevticheskii arkhiv
pub_type: 临床试验,杂志文章
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更新日期:2016-01-01 00:00:00
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journal_title:Terapevticheskii arkhiv
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doi:10.17116/terarkh201587348-52
更新日期:2015-01-01 00:00:00
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journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00