Abstract:
BACKGROUND:Normothermic ex vivo lung perfusion (EVLP) has been used successfully to evaluate and recondition marginal donor lungs; however, multiple barriers continue to prevent its widespread adoption. We sought to develop a common hospital ingredient-derived perfusate (CHIP) with equivalent functional and inflammatory characteristics to a standard Krebs-Henseleit buffer with 8% serum albumin-derived perfusate (KHB-Alb) to improve access and reduce costs of ex vivo organ perfusion. METHODS:Sixteen porcine lungs were perfused using negative pressure ventilation (NPV) EVLP for 12 hours in a normothermic state and were allocated equally to 2 groups: KHB-Alb vs CHIP. Physiological parameters, cytokine profiles, and edema formation were compared between treatment groups. RESULTS:Perfused lungs in both groups demonstrated equivalent oxygenation (partial pressure of arterial oxygen/fraction of inspired oxygen ratio >350 mm Hg) and physiological parameters. There was equivalent generation of tumor necrosis factor-α and IL-6, irrespective of perfusate solution used, when comparing CHIP vs KHB-Alb. Pig lungs developed equivalent edema formation between groups (CHIP: 15.8 ± 4.8%, KHB-Alb 19.5 ± 4.4%, P > .05). CONCLUSION:A perfusate derived of common hospital ingredients provides equivalent results to a standard Krebs-Henseleit buffer with 8% serum albumin-based perfusate in NPV-EVLP.
journal_name
Transplant Procjournal_title
Transplantation proceedingsauthors
Buchko MT,Himmat S,Aboelnazar NS,Stewart CJ,Hatami S,Dromparis P,Adam B,Freed DH,Nagendran Jdoi
10.1016/j.transproceed.2020.05.007subject
Has Abstractpub_date
2020-12-01 00:00:00pages
2941-2946issue
10eissn
0041-1345issn
1873-2623pii
S0041-1345(19)31455-1journal_volume
52pub_type
杂志文章abstract::The aim of this work was to understand the epidemiologic characteristics of TB and human immunodeficiency virus (HIV) dual infection cases, thus providing the basis for effective prevention and control measures. On the basis of HIV/TB screening of new and old TB and HIV/acquired immunodeficiency syndrome (AIDS) patien...
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2004-12-01 00:00:00
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