A Low-Cost Perfusate Alternative for Ex Vivo Lung Perfusion.

Abstract:

BACKGROUND:Normothermic ex vivo lung perfusion (EVLP) has been used successfully to evaluate and recondition marginal donor lungs; however, multiple barriers continue to prevent its widespread adoption. We sought to develop a common hospital ingredient-derived perfusate (CHIP) with equivalent functional and inflammatory characteristics to a standard Krebs-Henseleit buffer with 8% serum albumin-derived perfusate (KHB-Alb) to improve access and reduce costs of ex vivo organ perfusion. METHODS:Sixteen porcine lungs were perfused using negative pressure ventilation (NPV) EVLP for 12 hours in a normothermic state and were allocated equally to 2 groups: KHB-Alb vs CHIP. Physiological parameters, cytokine profiles, and edema formation were compared between treatment groups. RESULTS:Perfused lungs in both groups demonstrated equivalent oxygenation (partial pressure of arterial oxygen/fraction of inspired oxygen ratio >350 mm Hg) and physiological parameters. There was equivalent generation of tumor necrosis factor-α and IL-6, irrespective of perfusate solution used, when comparing CHIP vs KHB-Alb. Pig lungs developed equivalent edema formation between groups (CHIP: 15.8 ± 4.8%, KHB-Alb 19.5 ± 4.4%, P > .05). CONCLUSION:A perfusate derived of common hospital ingredients provides equivalent results to a standard Krebs-Henseleit buffer with 8% serum albumin-based perfusate in NPV-EVLP.

journal_name

Transplant Proc

authors

Buchko MT,Himmat S,Aboelnazar NS,Stewart CJ,Hatami S,Dromparis P,Adam B,Freed DH,Nagendran J

doi

10.1016/j.transproceed.2020.05.007

subject

Has Abstract

pub_date

2020-12-01 00:00:00

pages

2941-2946

issue

10

eissn

0041-1345

issn

1873-2623

pii

S0041-1345(19)31455-1

journal_volume

52

pub_type

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