Involvement of transcription repressor Snail in the regulation of human telomerase reverse transcriptase (hTERT) by transforming growth factor-β.

Abstract:

:Human telomerase reverse transcriptase (hTERT), a catalytic subunit of telomerase, is the primary determinant for telomerase enzyme activity, which has been associated with cellular immortality. Expression of the hTERT gene is regulated by various extracellular (external) stimuli and is aberrantly up-regulated in more than 90% of cancers. Here we show that hTERT gene expression was repressed in response to transforming growth factor-β (TGF-β) by a mechanism dependent on transcription factors Snail and c-Myc. TGF-β activated Snail and down-regulated c-Myc gene expression. In addition, ectopic expression of Snail strongly inhibited hTERT promoter activity, although co-expression of c-Myc abrogated this effect. Chromatin immunoprecipitation (ChIP) analysis revealed that TGF-β decreased c-Myc occupancy and dramatically increased recruitment of Snail to the E-box motifs of the hTERT promoter, thereby repressing hTERT expression. Our findings suggest a dynamic alteration in hTERT promoter occupancy by Snail and c-Myc is the mechanistic basis for TGF-β-mediated regulation of hTERT.

authors

Yoo YS,Park S,Gwak J,Ju BG,Oh S

doi

10.1016/j.bbrc.2015.07.146

subject

Has Abstract

pub_date

2015-09-11 00:00:00

pages

131-6

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(15)30376-4

journal_volume

465

pub_type

杂志文章
  • Bovine liver cDNA clones encoding a precursor of the alpha-subunit of the mitochondrial ATP synthase complex.

    abstract::cDNA clones encoding a precursor of the alpha-subunit of the mitochondrial ATP synthase complex have been isolated from a bovine liver cDNA library using the alpha-subunit gene from Saccharomyces cerevisiae as a probe. Analyses of the nucleotide sequence of these cDNA clones reveal that the bovine liver ATP synthase a...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(88)80709-5

    authors: Breen GA

    更新日期:1988-04-15 00:00:00

  • Calcium modulation of mitochondrial inner membrane channel activity.

    abstract::Protocols were defined that enable patch-clamp studies of the approximately 107 pS voltage dependent channel and a class of activity we refer to as MCC (multiconductance channel) which is characterized by multiple levels and transitions as high as 1 to 1.5 nS. If free calcium was kept at 10(-7) M or lower during mitoc...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(91)90410-9

    authors: Kinnally KW,Zorov D,Antonenko Y,Perini S

    更新日期:1991-05-15 00:00:00

  • Expression and characterization of recombinant kurtoxin, an inhibitor of T-type voltage-gated calcium channels.

    abstract::Kurtoxin, a 63-amino acid peptide stabilized by four disulfide bonds, is the first reported peptide inhibitor of T-type voltage-gated calcium channels. Although T-type calcium channels have been implicated in a number of disease states, including epilepsy, chronic pain, hypertension and cancer, the lack of selective i...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2011.11.003

    authors: Lee CW,Eu YJ,Min HJ,Cho EM,Lee JH,Kim HH,Nah SY,Swartz KJ,Kim JI

    更新日期:2011-12-16 00:00:00

  • Covalent conjugation of Groucho with SUMO-1 modulates its corepressor activity.

    abstract::Groucho is a corepressor that forms a macromolecular complex for its corepressor activity, in which HDAC1 is an essential component for the modulation of chromatin structure and transcriptional repression of target genes. Here, we show that Groucho is covalently conjugated with small ubiquitin-related modifier-1 (SUMO...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.12.043

    authors: Ahn JW,Lee YA,Ahn JH,Choi CY

    更新日期:2009-01-30 00:00:00

  • Characterization of the platelet-derived growth factor beta-receptor kinase activity by use of synthetic peptides.

    abstract::Synthetic peptides derived from the sequence surrounding tyrosine-857 in the human platelet-derived growth factor (PDGF) beta-receptor were used to elucidate the requirement for length and presence of negative and positively charged amino acids in substrates of the PDGF beta-receptor protein tyrosine kinase. The measu...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(90)90669-e

    authors: Rönnstrand L,Sorokin A,Engström U,Heldin CH

    更新日期:1990-03-30 00:00:00

  • Loss of virulence in NAD(P)H cytochrome b5 oxidoreductase deficient Leishmania major.

    abstract::Leishmania promastigotes have the ability to synthesize essential polyunsaturated fatty acids de novo and can grow in lipid free media. Recently, we have shown that NAD(P)H cytochrome b5 oxidoreductase (Ncb5or) enzyme in Leishmania acts as the redox partner for Δ12 fatty acid desaturase, which catalyses the conversion...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2018.06.037

    authors: Mukherjee A,Adhikari A,Das P,Biswas S,Mukherjee S,Adak S

    更新日期:2018-09-03 00:00:00

  • Functional interaction of poly(ADP-ribose) with the 20S proteasome in vitro.

    abstract::The nuclear enzyme poly(ADP-ribosyl) transferase (pADPRT) catalyzes the formation of poly(ADP-ribose) from NAD+. Several nuclear proteins and pADPRT itself are targets for the modification by poly(ADP-ribosyl)ation. It is demonstrated here that poly(ADP-ribose) or pADPRT automodified with poly(ADP-ribose) interacts no...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1999.0824

    authors: Mayer-Kuckuk P,Ullrich O,Ziegler M,Grune T,Schweiger M

    更新日期:1999-06-16 00:00:00

  • Properties and secondary structure analysis of BanI endonuclease: identification of putative active site.

    abstract::Biochemical properties of Type II restriction enzyme BanI were characterized. Kinetic parameters were evaluated and an enhancement of rate was observed when the recognition site was located in a more central position in the substrate, suggesting that BanI locates its recognition site by a sliding mechanism. As BanI ha...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2000.3621

    authors: Advani S,Roy KB

    更新日期:2000-12-09 00:00:00

  • Inorganic phosphate regulates CryIVA protoxin expression in Bacillus thuringiensis israelensis.

    abstract::The role of nutritional factors during CryIVA protoxin expression in Bacillus thuringiensis israelensis (Bti) has been investigated. Inorganic phosphate (Pi) was found to stimulate 135 kD protoxin synthesis by Bti cells. There was a corresponding increase in the cryIVA specific mRNA in the presence of Pi. Inorganic ph...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1999.1094

    authors: Banerjee-Bhatnagar N

    更新日期:1999-08-27 00:00:00

  • Domain architecture of the atypical Arf-family GTPase Arl13b involved in cilia formation.

    abstract::Arl13b is an atypical Arf/Arl-family GTPase consisting of an extending large C-terminal region (C domain) and Arf-homologous GTP-binding motifs in the N terminus (N domain). Although Arl13b appears to be involved in cilia formation, its precise function and roles of the domains remain unknown. Here, we show the unique...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.06.001

    authors: Hori Y,Kobayashi T,Kikko Y,Kontani K,Katada T

    更新日期:2008-08-15 00:00:00

  • Phosphoramidon, a metalloproteinase inhibitor, suppresses the secretion of endothelin-1 from cultured endothelial cells by inhibiting a big endothelin-1 converting enzyme.

    abstract::Time-dependent secretion of immunoreactive-endothelin (IR-ET) from cultured porcine aortic endothelial cells was markedly suppressed by phosphoramidon is due to proteinase inhibitor. Analysis of the culture supernatant with or without phosphoramidon by reverse-phase high performance liquid chromatography confirmed tha...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(90)91198-2

    authors: Ikegawa R,Matsumura Y,Tsukahara Y,Takaoka M,Morimoto S

    更新日期:1990-09-14 00:00:00

  • The role of lipopolysaccharide moieties in macrophage response to Escherichia coli.

    abstract::Lipopolysaccharide (LPS) is the main component of Gram-negative bacteria that - upon infection - activates the host immune system and is crucial in fighting pathogens as well as in the induction of sepsis. In the present study we addressed the question whether the key structural components of LPS equally take part in ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2009.08.082

    authors: Eder K,Vizler C,Kusz E,Karcagi I,Glavinas H,Balogh GE,Vigh L,Duda E,Gyorfy Z

    更新日期:2009-11-06 00:00:00

  • Purification and partial sequencing of human placental alkaline phosphatase.

    abstract::Two forms of human placental alkaline phosphatase have been purified to homogeneity utilizing high performance liquid chromatography. Both have the same amino acid composition but they differ in their carbohydrate substituents. Sequence data indicate that the two forms are identical for the first forty two residues fr...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(83)80252-6

    authors: Ezra E,Blacher R,Udenfriend S

    更新日期:1983-11-15 00:00:00

  • Down-regulation of integrin alpha M beta 2 ligand-binding function by the urokinase-type plasminogen activator receptor.

    abstract::The cell adhesion molecule integrin alphaMbeta2 associates with the urokinase-type plasminogen activator receptor (uPAR) on monocytes and neutrophils. uPAR also associates with members of the beta1 and beta3 integrins, and it modulates the ligand-binding function of these integrins. In this study, we showed that co-ex...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2006.07.179

    authors: Tang ML,Kong LS,Law SK,Tan SM

    更新日期:2006-09-29 00:00:00

  • D-galactosyl-beta1-1'-sphingosine and D-glucosyl-beta1-1'-sphingosine induce human natural killer cell apoptosis.

    abstract::Natural killer (NK) cells perform multiple biological functions including tumor cell lysis and eradicating virally infected cells. Here, we report for the first time that D-galactosyl-beta1-1' sphingosine and D-glucosyl-beta1- 1' sphingosine damage human NK cells. We show that these cells express T-cell-associated gen...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.06.027

    authors: Maghazachi AA,Knudsen E,Jin Y,Jenstad M,Chaudhry FA

    更新日期:2004-07-30 00:00:00

  • Lens-specific expression of a chicken beta A3/A1-crystallin promoter fragment in transgenic mice.

    abstract::Beta A3/A1-crystallin is one of the major refractive proteins of the chicken eye lens. Previously we showed that a fragment from -382 to +22 bp of the beta A3/A1-crystallin gene functions as a promoter in transfected lens cells. Here we show by use of the bacterial chloramphenicol acetyltransferase reporter gene that ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1996.0635

    authors: McDermott JB,Cvekl A,Piatigorsky J

    更新日期:1996-04-25 00:00:00

  • Bone morphogenetic proteins are involved in the pathobiology of synovial chondromatosis.

    abstract::Synovial chondromatosis is characterized by the formation of osteocartilaginous nodules (free bodies) under the surface of the synovial membrane in joints. Free bodies and synovium isolated from synovial chondromatosis patients expressed high levels of BMP-2 and BMP-4 mRNAs. BMP-2 stimulated the expression of Sox9, Co...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.12.170

    authors: Nakanishi S,Sakamoto K,Yoshitake H,Kino K,Amagasa T,Yamaguchi A

    更新日期:2009-02-20 00:00:00

  • Calmodulin and protein kinase C antagonists also inhibit the Ca2+-dependent protein protease, calpain I.

    abstract::The calmodulin and C-kinase antagonists melittin, calmidazolium, N-(6-aminohexyl)-5-chloro-1-napthalenesulfonamide (W7), and trifluoperazine (TFP) also inhibit the activity of the human erythrocyte Ca2+-dependent protease, calpain I. W-5, the nonchlorinated derivative of W-7, was ineffective as an inhibitor of calpain...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(89)92251-1

    authors: Brumley LM,Wallace RW

    更新日期:1989-03-31 00:00:00

  • Angiogenin is involved in morphological changes and angiogenesis in the ovary.

    abstract::Angiogenin is a potent angiogenic factor secreted by cultured tumor cells and is found in various normal organs and tissues. The ovary is one of the adult organs in which angiogenesis normally occurs during the female reproductive cycle. In this study, we examined whether angiogenin protein is localized and if angioge...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1999.0359

    authors: Lee HS,Lee IS,Kang TC,Jeong GB,Chang SI

    更新日期:1999-04-02 00:00:00

  • The acidic domain of hnRNPQ (NSAP1) has structural similarity to Barstar and binds to Apobec1.

    abstract::Apobec1 edits the ApoB mRNA by deaminating nucleotide C(6666), which results in a codon change from Glutamate to stop, and subsequent expression of a truncated protein. Apobec1 is regulated by ACF (Apobec1 complementation factor) and hnRNPQ, which contains an N-terminal "acidic domain" (AcD) of unknown function, three...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2006.09.044

    authors: Quaresma AJ,Oyama S Jr,Barbosa JA,Kobarg J

    更新日期:2006-11-17 00:00:00

  • Constitutive secretion of an endogenous insulin-like peptide binding protein with high affinity for insulin in Spodoptera frugiperda (Sf9) cell cultures.

    abstract::Serum-free medium from batch cultures of Sf9 insect cells was examined for the occurrence of proteins related to the insulin-like growth factor family. We found that the Sf9 cell line constitutively produced and secreted a soluble protein with a MW of 27 kDa that exerted specific binding to human insulin-like growth f...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1999.1745

    authors: Doverskog M,Tally M,Häggström L

    更新日期:1999-11-30 00:00:00

  • Joint cultivation of human erythroblastoid cells and mouse fibroblasts triggers release of a wide spectrum of cytotoxic factors.

    abstract::It has been demonstrated that joint cultivation of human K-562 cells and mouse L-929 cells results in triggering apoptosis in L-929 cells and possibly also in K-562 cells. Analysis of proteins recovered from the culture medium has revealed the presence of three cytotoxic factors including TNF-alpha. This is the first ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1997.6617

    authors: Gnuchev NV,Sashchenko LP,Iarovaia OV,Dukhanina E,Lukianova T,Razin SV

    更新日期:1997-05-29 00:00:00

  • Production and characterization of a novel site-specific-modifiable anti-OX40-receptor single-chain variable fragment for targeted drug delivery.

    abstract::OX40 receptor (tumor necrosis factor receptor superfamily, member 4; CD134) is a T-cell co-stimulatory molecule that plays an important role in T-cell activation and survival. OX40 receptor is activated by its ligand, OX40L; and modulation of the OX40-OX40L interaction is a promising target for the treatment of autoim...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2018.01.051

    authors: Tanabe A,Nakano K,Nakakido M,Nagatoishi S,Tanaka Y,Tsumoto K,Uchimaru K,Watanabe T

    更新日期:2018-02-05 00:00:00

  • Multiple processing forms and their biological activities of a novel angiogenesis inhibitor vasohibin.

    abstract::Vasohibin is a newly identified negative feedback regulator for angiogenesis. When expressed in cultured human endothelial cells, vasohibin polypeptides were detected in multiple distinct molecular weight forms, suggesting that some proteolytic events may occur within cells or the pericellular milieu. In order to iden...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2006.01.185

    authors: Sonoda H,Ohta H,Watanabe K,Yamashita H,Kimura H,Sato Y

    更新日期:2006-04-07 00:00:00

  • Superior serum half life of albumin tagged TNF ligands.

    abstract::Due to their immune stimulating and apoptosis inducing properties, ligands of the TNF family attract increasing interest as therapeutic proteins. A general limitation of in vivo applications of recombinant soluble TNF ligands is their notoriously rapid clearance from circulation. To improve the serum half life of the ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2010.04.134

    authors: Müller N,Schneider B,Pfizenmaier K,Wajant H

    更新日期:2010-06-11 00:00:00

  • Severe Fanconi Anemia phenotypes in Fancd2 depletion mice.

    abstract::Fanconi anemia (FA) is a genetic disorder characterized by congenital malfunction, bone marrow failure and hypersensitivity to DNA damage. FANCD2 protein play the central role in FA pathway. To study the in vivo role of FANCD2, we generated and characterized a new Fancd2 knockout mouse strain with 7bp deletion in Fanc...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2019.04.201

    authors: Yang Q,Xie H,Zhong Y,Li D,Ke X,Ying H,Yu B,Zhang T

    更新日期:2019-06-30 00:00:00

  • High D-glucose stimulates the cell cycle from the G1 to the S and M phases, but has no competent effect on the G0 phase, in vascular smooth muscle cells.

    abstract::The effects of high (28mM) D-glucose (HG) on the cell cycle progression were investigated in rat aorta vascular smooth muscle cells (VSMCs) in primary culture, using an immunocytochemical analysis of cell-cycle-specific nuclear antigens. HG had no effect on the cell cycle of the serum-deprived G0 cells, whereas platel...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1995.1858

    authors: Hiroishi G,Kobayashi S,Nishimura J,Inomata H,Kanaide H

    更新日期:1995-06-15 00:00:00

  • Tubulin polymerization promoting protein 1 (TPPP1) increases β-catenin expression through inhibition of HDAC6 activity in U2OS osteosarcoma cells.

    abstract::The Rho-associated coiled-coil kinase (ROCK) family of proteins, including ROCK1 and ROCK2, are key regulators of actin and intermediate filament morphology. The newly discovered ROCK substrate Tubulin polymerization promoting protein 1 (TPPP1) promotes microtubule polymerization and inhibits the activity of Histone d...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2013.05.076

    authors: Schofield AV,Gamell C,Bernard O

    更新日期:2013-07-12 00:00:00

  • Identification of differentially expressed genes in human heart with ventricular septal defect using suppression subtractive hybridization.

    abstract::Ventricular septal defect (VSD) accounts for the largest number of birth congenital heart defects in human, but the genetic programs that control ventricular septation are poorly understood. To identify differentially expressed genes between ventricular septal defect and normal ventricular septum myocardium, we have u...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2006.01.113

    authors: Zhang H,Zhou L,Yang R,Sheng Y,Sun W,Kong X,Cao K

    更新日期:2006-03-31 00:00:00

  • New partner proteins containing novel internal recognition motif for human glutaminase interacting protein (hGIP).

    abstract::Regulation of gene expression in cells is mediated by protein-protein, DNA-protein and receptor-ligand interactions. PDZ (PSD-95/Discs-large/ZO-1) domains are protein-protein interaction modules. PDZ-containing proteins function in the organization of multi-protein complexes controlling spatial and temporal fidelity o...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2013.01.098

    authors: Zencir S,Banerjee M,Dobson MJ,Ayaydin F,Fodor EA,Topcu Z,Mohanty S

    更新日期:2013-03-01 00:00:00