Abstract:
:Lignocellulosic biomass is a renewable and sustainable energy source. Cellulases are the enzymes that cleave β-1, 4-glycosidic linkages in cellulose to liberate sugars that can be fermented to ethanol, butanol, and other products. Low enzyme activity and yield, and thermostability are, however, some of the limitations posing hurdles in saccharification of lignocellulosic residues. Recent advancements in synthetic and systems biology have generated immense interest in metabolic and genetic engineering that has led to the development of sustainable technology for saccharification of lignocellulosics in the last couple of decades. There have been several attempts in applying genetic engineering in the production of a repertoire of cellulases at a low cost with a high biomass saccharification. A diverse range of cellulases are produced by different microbes, some of which are being engineered to evolve robust cellulases. This review summarizes various successful genetic engineering strategies employed for improving cellulase kinetics and cellulolytic efficiency.
journal_name
Front Microbioljournal_title
Frontiers in microbiologyauthors
Dadwal A,Sharma S,Satyanarayana Tdoi
10.3389/fmicb.2020.01387subject
Has Abstractpub_date
2020-06-24 00:00:00pages
1387issn
1664-302Xjournal_volume
11pub_type
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更新日期:2017-05-17 00:00:00
abstract::Lactic acid bacteria (LAB) are important for human health. However, the relative abundance of LAB in complex samples, such as fecal samples, is low and their presence and diversity (at the species level) is understudied. Therefore, we designed LAB-specific primer pairs based on 16S rRNA gene consensus sequences from 4...
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doi:10.3389/fmicb.2020.01860
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abstract::Atypical porcine pestivirus (APPV) is an RNA virus newly discovered from swine in Asia, Europe, and the Americas. This novel virus has been confirmed as the cause of congenital tremor (CT) in piglets, which causes extensive economic losses to the swine industry. To investigate the genetic diversity and evolutionary re...
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pub_type: 杂志文章
doi:10.3389/fmicb.2019.01406
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pub_type: 杂志文章
doi:10.3389/fmicb.2019.00260
更新日期:2019-02-25 00:00:00
abstract::Species of Trichoderma exert direct biocontrol activity against soil-borne plant pathogens due to their ability to compete for nutrients and to inhibit or kill their targets through the production of antibiotics and/or hydrolytic enzymes. In addition to these abilities, Trichoderma spp. have beneficial effects for pla...
journal_title:Frontiers in microbiology
pub_type: 杂志文章
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journal_title:Frontiers in microbiology
pub_type: 杂志文章,评审
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更新日期:2017-09-29 00:00:00
abstract::Infections that reach the placenta via maternal blood can target the fetal-placental barrier and are associated with reduced birth weight, increased stillbirth, miscarriage and perinatal mortality. Malaria during pregnancy can lead to infection of the placental tissue and to adverse effects on the unborn child even if...
journal_title:Frontiers in microbiology
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doi:10.3389/fmicb.2019.01126
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:2020-05-20 00:00:00
abstract::The success of Ericaceae in stressful habitats enriched in heavy metals has been ascribed to the distinctive abilities of their mycorrhizal fungal partners to withstand heavy metal stress and to enhance metal tolerance in the host plant. Whereas heavy metal tolerance has been extensively investigated in some ericoid m...
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pub_type: 杂志文章
doi:10.3389/fmicb.2020.00341
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abstract::Wheezing is a lower respiratory tract symptom induced by various viral respiratory infections. Epidemiological studies have revealed the phenotypes of wheezing in early childhood which have different risk factors for the development of asthma among school age children. The major viral species causing wheezing in child...
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doi:10.3389/fmicb.2019.01255
更新日期:2019-06-05 00:00:00