Ketimine reductase/CRYM catalyzes reductive alkylamination of α-keto acids, confirming its function as an imine reductase.

Abstract:

:Recently, crystalized mouse ketimine reductase/CRYM complexed with NADPH was found to have pyruvate bound in its active site. We demonstrate that the enzyme binds α-keto acids, such as pyruvate, in solution, and catalyzes the formation of N-alkyl-amino acids from alkylamines and α-keto acids (via reduction of imine intermediates), but at concentrations of these compounds not expected to be encountered in vivo. These findings confirm that, mechanistically, ketimine reductase/CRYM acts as a classical imine reductase and may explain the finding of bound pyruvate in the crystallized protein.

journal_name

Amino Acids

journal_title

Amino acids

authors

Hallen A,Cooper AJ,Smith JR,Jamie JF,Karuso P

doi

10.1007/s00726-015-2044-8

subject

Has Abstract

pub_date

2015-11-01 00:00:00

pages

2457-61

issue

11

eissn

0939-4451

issn

1438-2199

pii

10.1007/s00726-015-2044-8

journal_volume

47

pub_type

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