Abstract:
:Recently, crystalized mouse ketimine reductase/CRYM complexed with NADPH was found to have pyruvate bound in its active site. We demonstrate that the enzyme binds α-keto acids, such as pyruvate, in solution, and catalyzes the formation of N-alkyl-amino acids from alkylamines and α-keto acids (via reduction of imine intermediates), but at concentrations of these compounds not expected to be encountered in vivo. These findings confirm that, mechanistically, ketimine reductase/CRYM acts as a classical imine reductase and may explain the finding of bound pyruvate in the crystallized protein.
journal_name
Amino Acidsjournal_title
Amino acidsauthors
Hallen A,Cooper AJ,Smith JR,Jamie JF,Karuso Pdoi
10.1007/s00726-015-2044-8subject
Has Abstractpub_date
2015-11-01 00:00:00pages
2457-61issue
11eissn
0939-4451issn
1438-2199pii
10.1007/s00726-015-2044-8journal_volume
47pub_type
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