Abstract:
:Amitraz is a formamidine pesticide that has been reported to be a neurotoxic compound that induces convulsions among other effects. Excitatory and inhibitory neurotransmission is mediated mainly by glutamate and GABA, respectively, so their alteration could be responsible for induction of seizures. In this regard, amitraz α2 adrenergic agonist action, which has been suggested as likely responsible for this effect, could alter these neurotransmitter systems and lead to seizure induction. Moreover, other amitraz mechanisms such as histamine H1 receptor inhibition could be involved. To confirm if amitraz disrupts glutamatergic/GABAergic transmission by these mechanisms, we evaluated, in primary hippocampal neurons, the effect of amitraz (0.01 μM to 100 μM) with or without the α2 adrenergic antagonist idazoxan (1 μM) and/or the H1 receptor agonist n-methylhistaprodifen (30 μM) co-treatment on 4-aminobutyrate aminotransferase, glutamate decarboxylase 65 (GAD 65), succinate-semialdehyde dehydrogenase and glutaminase gene expression and on glutamate and GABA levels after 24h treatment. We observed that amitraz disrupts glutaminase and GAD 65 gene expression, altering glutamatergic and GABAergic transmission. These effects were mediated partially by H1 and α2 receptors suggesting that other mechanisms could be involved. These data could help explain the mechanism by which amitraz induces seizures and provide a therapeutic strategy to protect against this effect in case of poisoning.
journal_name
Neurotoxicol Teratoljournal_title
Neurotoxicology and teratologyauthors
Del Pino J,Frejo MT,Baselga MJ,Moyano P,Díaz MJdoi
10.1016/j.ntt.2015.06.007subject
Has Abstractpub_date
2015-07-01 00:00:00pages
82-7eissn
0892-0362issn
1872-9738pii
S0892-0362(15)30009-Xjournal_volume
50pub_type
杂志文章abstract::With the implementation of the Food Quality Protection Act in 1996, more detailed evaluations of possible health effects of pesticides on developing organisms have been required. As a result, considerable developmental neurotoxicity (DNT) data have been generated on a variety of endpoints, including developmental chan...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章,评审
doi:10.1016/j.ntt.2007.12.009
更新日期:2008-07-01 00:00:00
abstract::Zidovudine (AZT) is the main therapeutic agent against HIV vertical transmission and is routinely administered to seropositive pregnant women and their newborns. Toxicity after chronic administration as well as citogenetic effects following developmental AZT exposure has been reported. Furthermore, recent animal data ...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/j.ntt.2004.09.008
更新日期:2005-01-01 00:00:00
abstract::Male Fischer 344 rats received either tap water or water containing 250 ppm lead for 90 days prior to training in either Pavlovian fear conditioning or consummatory contrast, an aversive reward reduction paradigm. In Experiment 1, lead-exposed and -unexposed rats were trained in operant chambers over a 6-min session. ...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/s0892-0362(02)00265-9
更新日期:2002-07-01 00:00:00
abstract::Pregnant Sprague-Dawley rats were fed a liquid diet containing either 0% (group C), 18% (group L), or 36% (group H) ethanol-derived calories (EDC) from gestational day 1 to 20. Male offspring were assessed under a conditioned taste aversion paradigm (PND 35-45), in a complex maze (PND 68-80), and for operant behavior ...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/0892-0362(95)00014-i
更新日期:1995-09-01 00:00:00
abstract::While there is a long-standing tradition of using behavioral methods to study the effects of manufactured drugs and environmental chemicals, comparatively little attention has focused until recently on the behavioral effects of marine or freshwater toxins. A vast array of microorganisms, found in a variety of waters, ...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章,评审
doi:10.1016/j.ntt.2005.06.007
更新日期:2005-09-01 00:00:00
abstract::Two hundred thirty-six children from two established cohorts at risk for exposure to polychlorinated biphenyls (PCBs) and related contaminants were evaluated at age 4 years. Prenatal exposure (assessed by cord serum PCB level) was associated with lower weight, an effect consistent with reports of growth retardation in...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/0892-0362(90)90050-m
更新日期:1990-07-01 00:00:00
abstract::Disruptive Behavior Disorders (DBD) exhibit a sex-biased prevalence rate favoring boys, and prenatal testosterone exposure appears to be part of the complex etiology of these disorders. The current study examines whether high prenatal testosterone exposure may heighten the risk for DBD symptoms in males by increasing ...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/j.ntt.2014.05.001
更新日期:2014-07-01 00:00:00
abstract::While the toxic effects of lead have been recognized for millennia, it has remained a significant public health concern due to its continued use and toxicological potential. Of particular interest is the increased susceptibility of young children to the toxic effects of lead. Although the exact mechanism(s) for lead t...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/j.ntt.2011.05.006
更新日期:2011-07-01 00:00:00
abstract::The increased use of cocaine in the United States and worldwide has created great concern about its effects on fetuses and neonates of pregnant cocaine abusers. The effects on neonates are varied: fetal growth delay, microcephaly, abnormal neurological functions, microphthalmia, and maternal obstetric complications. I...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/s0892-0362(96)00227-9
更新日期:1997-05-01 00:00:00
abstract::One hundred and ten, 6- to 11-year-old children from a low-risk, predominantly middle class sample who are participants in an ongoing longitudinal drug study were assessed using a central auditory processing task (SCAN) that made perceptual rather than linguistic demands. Maternal smoking during pregnancy was linearly...
journal_title:Neurotoxicology and teratology
pub_type: 临床试验,杂志文章
doi:10.1016/0892-0362(94)90048-5
更新日期:1994-05-01 00:00:00
abstract::Previous work showed that rats develop tolerance to the acute behavioral effects of trichloroethylene (TCE) on signal detection if they inhale TCE while performing the task and that this tolerance depends more upon learning than upon changes in metabolism of TCE. The present study sought to characterize this tolerance...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/s0892-0362(01)00182-9
更新日期:2001-11-01 00:00:00
abstract::We have utilized a mouse model of transplacental cocaine exposure to investigate the effects of cocaine dose and gestational timing in altering brain and body growth and postnatal behavior in exposed offspring. Pregnant dams were injected with cocaine HCl at 40 mg/kg/day (COC 40) or 20 mg/kg/day (COC 20), or 10 mg/kg/...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/s0892-0362(97)00127-x
更新日期:1998-05-01 00:00:00
abstract::The interest in, and number of, neurobehavioral test batteries for use in occupational settings has increased markedly over the last decade. While this is a welcome development in furthering the cause for greater acceptance of these methods in hazard evaluation and toxicity testing, there are a number of issues that a...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/0892-0362(90)90015-5
更新日期:1990-09-01 00:00:00
abstract::1-beta-D-Arabinofuranosylcytosine (Ara-C), a cytidine analogue cytotoxic to proliferating cells, has a teratogenic effect in the brain of experimental animals and causes neural cell apoptosis in vitro and in vivo. In the present study, pregnant rats were injected with Ara-C on Day 13 of gestation and the fetal brain w...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/j.ntt.2004.03.010
更新日期:2004-07-01 00:00:00
abstract::Male Swiss mice, 25-30 g, were utilized to define some of the behavioral effects of the herbicides Lasso [alachlor 43%; (A)], Basalin [fluchloralin 45%; (F)], Premerge 3 [dinoseb 51%; (D)], and the fungicide Maneb-80 [maneb 80%; (M)]. These compounds were tested for their effects on locomotor activity and for their ab...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/0892-0362(89)90084-6
更新日期:1989-01-01 00:00:00
abstract::Measures of visual contrast sensitivity (VCS), rather than traditional measures of visual acuity using high-contrast stimuli, have been presented as better appraisals of visual dysfunction resulting from chemical exposures. The present study sought to determine if differences exist between two available measures of co...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/j.ntt.2003.10.007
更新日期:2004-03-01 00:00:00
abstract::Experimental models and clinical data indicate that the incidence of motor and learning disorders may be increased in children of epileptic mothers taking phenytoin (PHT) during pregnancy. There is little data on the vulnerability of infants to PHT-induced long-term behavioral toxicity after gestational or early life ...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/j.ntt.2004.12.006
更新日期:2005-03-01 00:00:00
abstract::Neurotoxicity has been described in workers exposed to solvents, PCBs, certain metals, and polyaromatic hydrocarbons but not often in residents near refineries or factories. We compared the neurobehavioral performance of residents near a plant that reprocessed used motor oil and chemical waste from 1966-1983 to refere...
journal_title:Neurotoxicology and teratology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0892-0362(94)00057-k
更新日期:1995-03-01 00:00:00
abstract::Long-Evans rats with micrencephaly induced by prenatal exposure to methylazoxymethanol acetate and normal controls were trained in a two-choice box to discriminate between stimuli of different brightness (black vs. white) or pattern (horizontal vs. vertical alternating black-and-white stripes). Mild footshock was used...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/0892-0362(90)90060-p
更新日期:1990-07-01 00:00:00
abstract::The study examines possible persisting effects on the peripheral nervous system and visual system in tunnel workers previously exposed to acrylamide and N-methylolacrylamide during grouting work. We compared neurophysiological function in 44 tunnel workers previously exposed during grouting operations (2-10 years post...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/j.ntt.2008.01.006
更新日期:2008-05-01 00:00:00
abstract::Brain-derived neurotrophic factor (BDNF) is a neurotrophin critical for many developmental and physiological aspects of CNS function. Severe hypothyroidism in the early neonatal period results in developmental and cognitive impairments and reductions in mRNA and protein expression of BDNF in a number of brain regions....
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/j.ntt.2011.04.001
更新日期:2011-07-01 00:00:00
abstract::Lead is a persistent metal and commonly present in our living environment. The present study was aimed to investigate lead-induced embryonic toxicity, behavioral responses, and adult learning/memory deficit in zebrafish. Lead acetate (PbAc) induced malformations such as uninflated swim bladder, bent spine and yolk-sac...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/j.ntt.2012.09.001
更新日期:2012-11-01 00:00:00
abstract::The central and peripheral nervous system effects of acute and subchronic exposure to three organic phosphoro-acid esters (dimethoate, dichlorvos, parathion-methyl) were studied. CNS-dependent variables included mean EEG amplitude, mean frequency of the EEG, and the activity (power density) of six component frequency ...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/0892-0362(88)90004-9
更新日期:1988-09-01 00:00:00
abstract::Fifty nondrug-exposed infants and 74 cocaine/polydrug-exposed infants were evaluated on the Movement Assessment of Infants (MAI). The test provides an assessment of risk for motor dysfunction at age 4 months. There was a significant difference in total risk scores between the two groups of infants with cocaine-exposed...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/0892-0362(92)90057-h
更新日期:1992-03-01 00:00:00
abstract::Hazard information essential to guide developmental neurotoxicity risk assessments is limited for many chemicals. As developmental neurotoxicity testing using rodents is laborious and expensive, alternative species such as zebrafish are being adapted for rapid toxicity screening. Assessing the developmental neurotoxic...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/j.ntt.2009.04.065
更新日期:2010-01-01 00:00:00
abstract::Risk assessment of developmental toxicants is almost exclusively based on single chemicals studied in animals under controlled experimental conditions, as to reduce stress. Although humans may be exposed simultaneously to numerous hazards, little is known about the interaction of prenatal chemical exposures with other...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章,评审
doi:10.1016/j.ntt.2007.02.003
更新日期:2007-07-01 00:00:00
abstract:OBJECTIVE:To examine the association between prenatal exposure to nicotine from tobacco smoke and infant neurobehavior using tobacco biomarkers and a sensitive and comprehensive measure of infant neurobehavior. STUDY DESIGN:Participants were 318 infants (206 White, 95 Black, 17 Other) and their mothers. Prenatal tobac...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/j.ntt.2009.07.004
更新日期:2009-11-01 00:00:00
abstract::The effect of inhalation exposure to toluene (3700 mg/m3, 1000 ppm, 21 h/day, 5 days/week, during 4 weeks) on male Sprague-Dawley rats was tested. A wide range of test situations was used, including an operant test with baseline performance and extinction, motor coordination, and exploratory activity. All tests were m...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/0892-0362(91)90052-x
更新日期:1991-09-01 00:00:00
abstract::Research suggests that not only is marijuana use prevalent among women of reproductive age, but a significant number of women continue to use marijuana and its derivatives throughout pregnancy. Many studies have shown, in both humans and animals, that marijuana exposure during adolescence and adulthood is detrimental ...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/j.ntt.2011.10.006
更新日期:2012-01-01 00:00:00
abstract::Computer competition analysis of 3H-DHA (3H-dihydroalprenolol, a nonselective beta-adrenergic radioligand) binding in the presence of unlabeled metoprolol (a beta 1-selective antagonist) indicates the existence of both beta 1- and beta 2-adrenergic receptor subtypes in the rat placenta and confirms previous reports th...
journal_title:Neurotoxicology and teratology
pub_type: 杂志文章
doi:10.1016/0892-0362(91)90026-s
更新日期:1991-01-01 00:00:00