DNA Content in Ischemic Stroke Thrombi Can Help Identify Cardioembolic Strokes Among Strokes of Undetermined Cause.

Abstract:

BACKGROUND AND PURPOSE:Identification of acute ischemic stroke (AIS) cause is crucial for guidance of secondary prevention. Previous studies have yielded inconsistent results regarding possible correlations between AIS cause and thrombus composition, as assessed by semiquantitative histological analysis. Here, we performed a correlation analysis between AIS cause and AIS thrombus cellular composition and content, as assessed using quantitative biochemical assays. METHODS:Homogenates of 250 patients with AIS thrombi were prepared by mechanical grinding. Platelet, red blood cell, and leukocyte content of AIS thrombi were estimated by quantification of GP (glycoprotein) VI, heme, and DNA in thrombus homogenates. AIS cause was defined as cardioembolic, noncardioembolic, or embolic stroke of undetermined source, according to the TOAST classification (Trial of ORG 10172 in Acute Stroke Treatment). RESULTS:Cardioembolic thrombi were richer in DNA (35.8 versus 13.8 ng/mg, P<0.001) and poorer in GPVI (0.104 versus 0.117 ng/mg, P=0.045) than noncardioembolic ones. The area under the receiver operating characteristic curve of DNA content to discriminate cardioembolic thrombi from noncardioembolic was 0.72 (95% CI, 0.63-0.81). With a threshold of 44.7 ng DNA/mg thrombus, 47% of thrombi from undetermined cause would be classified as cardioembolic with a specificity of 90%. CONCLUSIONS:Thrombus DNA content may provide an accurate biomarker for identification of cardioembolic thrombi in patients with AIS with embolic stroke of undetermined source. Registration: URL: https://www.clinicaltrials.gov. Unique identifier: NCT03268668.

journal_name

Stroke

journal_title

Stroke

authors

Di Meglio L,Desilles JP,Solonomenjanahary M,Labreuche J,Ollivier V,Dupont S,Deschildre C,Maacha MB,Consoli A,Lapergue B,Piotin M,Blanc R,Ho-Tin-Noe B,Mazighi M,compoCLOT study group†.

doi

10.1161/STROKEAHA.120.029134

subject

Has Abstract

pub_date

2020-09-01 00:00:00

pages

2810-2816

issue

9

eissn

0039-2499

issn

1524-4628

journal_volume

51

pub_type

杂志文章

相关文献

STROKE文献大全
  • Cation activities in reversible ischemia of the cat brain.

    abstract::In normothermic anesthetized cats cerebral blood flow was interrupted completely for one hour by arterial clamping and induced hypotension. The effect of ischemia on the ionic gradients of the cerebral cortex was assayed by determining total cortical electrolytes and by recording the activities of extracellular potass...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/01.str.8.1.77

    authors: Hossmann KA,Sakaki S,Zimmerman V

    更新日期:1977-01-01 00:00:00

  • Homocysteine and pulsatility index of cerebral arteries.

    abstract:BACKGROUND AND PURPOSE:A pulsatility index (PI) represents vascular resistance distal to an examined artery. The purpose of the present study was to evaluate an association between plasma total homocysteine (tHcy) and PIs of the cerebral arteries in patients with ischemic stroke. METHODS:Consecutive patients with isch...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/STROKEAHA.109.558403

    authors: Lim MH,Cho YI,Jeong SK

    更新日期:2009-10-01 00:00:00

  • Late outcome of patients with premature carotid atherosclerosis after carotid endarterectomy.

    abstract:BACKGROUND AND PURPOSE:The durability of carotid endarterectomy (CEA) in young adults with premature carotid atherosclerosis has not been adequately assessed. This study examined the late recurrence and mortality rates in young adults undergoing CEA. METHODS:We studied 42 young patients (mean age, 45.5 +/- 0.5 years) ...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/01.str.27.9.1502

    authors: Valentine RJ,Myers SI,Hagino RT,Clagett GP

    更新日期:1996-09-01 00:00:00

  • Reliability of the National Institutes of Health Stroke Scale. Extension to non-neurologists in the context of a clinical trial.

    abstract:BACKGROUND AND PURPOSE:The reliability of the National Institutes of Health Stroke Scale (NIHSS) has been established through testing its use in live and videotaped patients. This reliability testing has primarily focused on the use of the scale by neurologists. We sought to determine the reliability of the NIHSS as us...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/01.str.28.2.307

    authors: Goldstein LB,Samsa GP

    更新日期:1997-02-01 00:00:00

  • Vascular Tissue-Type Plasminogen Activator Promotes Intracranial Aneurysm Formation.

    abstract:BACKGROUND AND PURPOSE:Although the mechanisms that contribute to intracranial aneurysm (IA) formation and rupture are not totally elucidated, inflammation and matrix remodeling are incriminated. Because tPA (tissue-type plasminogen activator) controls both inflammatory and matrix remodeling processes, we hypothesized ...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/STROKEAHA.117.017305

    authors: Labeyrie PE,Goulay R,Martinez de Lizarrondo S,Hébert M,Gauberti M,Maubert E,Delaunay B,Gory B,Signorelli F,Turjman F,Touzé E,Courthéoux P,Vivien D,Orset C

    更新日期:2017-09-01 00:00:00

  • Gene therapy for cerebral vascular disease.

    abstract:BACKGROUND:Gene transfer to peripheral arteries has been accomplished with catheter-based approaches. Recently, gene transfer to the carotid artery and intracranial vessels has been achieved both in vitro and in vivo. Although gene therapy for cerebral vascular disease may not be accomplished for years, currently avail...

    journal_title:Stroke

    pub_type: 杂志文章,评审

    doi:10.1161/01.str.27.9.1688

    authors: Heistad DD,Faraci FM

    更新日期:1996-09-01 00:00:00

  • Low-Dose Tirofiban Improves Functional Outcome in Acute Ischemic Stroke Patients Treated With Endovascular Thrombectomy.

    abstract:BACKGROUND AND PURPOSE:Chinese patients largely experience acute ischemic stroke (AIS) because of large artery atherosclerosis rather than cardioembolism, and whether tirofiban is safe and effective in these patients treated with endovascular thrombectomy (ET) is unknown. This study evaluated the safety and efficacy of...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/STROKEAHA.117.019193

    authors: Zhao W,Che R,Shang S,Wu C,Li C,Wu L,Chen J,Duan J,Song H,Zhang H,Ling F,Wang Y,Liebeskind D,Feng W,Ji X

    更新日期:2017-12-01 00:00:00

  • Flow and compartmental weight in relation to the cours of stroke.

    abstract::Using a scintillation camera system, hemispheric and regional cerebral blood flow was measured repeatedly during the course after a stroke. In 20 patients who improved clinically mean hemispheric and regional flow and relative weight of rapidly perfused compartment increased, while these values decreased in 15 patient...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/01.str.7.4.399

    authors: Heiss WD,Zeiler K,Turnheim M,Podreka I

    更新日期:1976-07-01 00:00:00

  • Human neural stem cell grafts modify microglial response and enhance axonal sprouting in neonatal hypoxic-ischemic brain injury.

    abstract:BACKGROUND AND PURPOSE:Hypoxic-ischemic (HI) brain injury in newborn infants represents a major cause of cerebral palsy, development delay, and epilepsy. Stem cell-based therapy has the potential to rescue and replace the ischemic tissue caused by HI and to restore function. However, the mechanisms by which stem cell t...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/STROKEAHA.109.573691

    authors: Daadi MM,Davis AS,Arac A,Li Z,Maag AL,Bhatnagar R,Jiang K,Sun G,Wu JC,Steinberg GK

    更新日期:2010-03-01 00:00:00

  • Role of transcranial Doppler and stump pressure during carotid endarterectomy.

    abstract:BACKGROUND AND PURPOSE:The aim of our study was to clarify the pathophysiology of perioperative cerebral complications during carotid endarterectomy in our series. METHODS:By means of transcranial Doppler ultrasonography and stump pressure measurement, we monitored 112 patients who underwent carotid endarterectomy und...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/01.str.28.12.2448

    authors: Finocchi C,Gandolfo C,Carissimi T,Del Sette M,Bertoglio C

    更新日期:1997-12-01 00:00:00

  • Corpus callosum and experimental stroke: studies in callosotomized rats and acallosal mice.

    abstract:BACKGROUND AND PURPOSE:Interhemispheric inhibition via the corpus callosum has been proposed as an exacerbating factor in outcome from stroke. METHODS:We measured infarct volume and behavioral outcome after middle cerebral artery occlusion in callosotomized rats and acallosal mice. RESULTS:Neither callosotomy in rats...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/STROKEAHA.111.613349

    authors: Jin K,Xie L,Sun F,Mao X,Greenberg DA

    更新日期:2011-09-01 00:00:00

  • Spinal cord infarction: a highly reproducible stroke model.

    abstract::The study of focal central nervous system ischemia has been impeded by the lack of animal models that are both reproducible and simulate human ischemic strokes. To circumvent these problems, we have developed a rabbit spinal cord infarction model. Infarction of the caudal lumbar cord is produced by temporary occlusion...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/01.str.11.2.200

    authors: Zivin JA,DeGirolami U

    更新日期:1980-03-01 00:00:00

  • Changes in locomotor activity and passive avoidance task performance induced by cerebral ischemia in Mongolian gerbils.

    abstract:BACKGROUND AND PURPOSE:We investigated changes in locomotor activity, passive avoidance task performance, and hippocampal CA1 neurons induced by cerebral ischemia in Mongolian gerbils to examine the relation between these behavioral changes and CA1 neuronal damage. METHODS:Spontaneous locomotor activity was measured u...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/01.str.25.3.645

    authors: Karasawa Y,Araki H,Otomo S

    更新日期:1994-03-01 00:00:00

  • Noninvasive assessment of cerebral collateral blood supply through the ophthalmic artery.

    abstract::We assessed the potential of 2-MHz pulsed-wave transorbital Doppler ultrasonography to delineate the role of the ophthalmic artery as a source of collateral cerebral blood supply by comparing oculopneumoplethysmography, transorbital Doppler ultrasonography, periorbital continuous-wave Doppler ultrasonography, and tran...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/01.str.22.1.31

    authors: Schneider PA,Rossman ME,Bernstein EF,Ringelstein EB,Otis SM

    更新日期:1991-01-01 00:00:00

  • Dissemination of thrombolysis for acute ischemic stroke across a nation: experiences from the Swedish stroke register, 2003 to 2008.

    abstract:BACKGROUND AND PURPOSE:We used Riks-Stroke, the Swedish Stroke Register, to explore how thrombolysis has been disseminated in Swedish hospitals since it was approved in 2003. METHODS:All 78 hospitals in Sweden admitting patients with acute stroke participate in Riks-Stroke. Between 2003 and 2008, 72 033 adult patients...

    journal_title:Stroke

    pub_type: 杂志文章,多中心研究

    doi:10.1161/STROKEAHA.109.577106

    authors: Eriksson M,Jonsson F,Appelros P,Asberg KH,Norrving B,Stegmayr B,Terént A,Asplund K,Riks-Stroke Collaboration.

    更新日期:2010-06-01 00:00:00

  • Cerebral infarction immediately after ingestion of hydrogen peroxide solution.

    abstract:BACKGROUND:We report the clinical and neuroimaging findings of a patient who sustained multiple cerebral infarcts after the ingestion of concentrated hydrogen peroxide solution sold as a "health food" product. CASE DESCRIPTION:An 84-year-old man sustained focal neurological deficits immediately after ingesting 30 mL o...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/01.str.25.5.1065

    authors: Sherman SJ,Boyer LV,Sibley WA

    更新日期:1994-05-01 00:00:00

  • Intakes of potassium, magnesium, and calcium and risk of stroke.

    abstract:BACKGROUND AND PURPOSE:We aimed to investigate the associations of dietary and total potassium, magnesium, and calcium intakes with stroke occurrence. METHODS:A prospective cohort study was conducted among 36 094 participants aged 21 to 70 years. Dietary intake was assessed with a food frequency questionnaire. RESULT...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/STROKEAHA.113.004032

    authors: Sluijs I,Czernichow S,Beulens JW,Boer JM,van der Schouw YT,Verschuren WM,Grobbee DE

    更新日期:2014-04-01 00:00:00

  • What makes family caregivers happy during the first 2 years post stroke?

    abstract:BACKGROUND AND PURPOSE:This study aimed to identify aspects of the caregiving situation contributing to family caregivers' psychological well-being. METHODS:Longitudinal cohort study with structured quantitative interviews 1, 3, 6, and 12 months post stroke. A subset of participants also completed surveys 18 and 24 mo...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/STROKEAHA.113.004309

    authors: Cameron JI,Stewart DE,Streiner DL,Coyte PC,Cheung AM

    更新日期:2014-04-01 00:00:00

  • Does the mismatch match the penumbra? Magnetic resonance imaging and positron emission tomography in early ischemic stroke.

    abstract:BACKGROUND AND PURPOSE:In ischemic stroke, diffusion-weighted (DW) and perfusion-weighted (PW) magnet resonance imaging (MRI) is used to define the mismatch as the therapeutic target. With positron emission tomography (PET), we characterized the metabolic patterns of tissue compartments identified by MRI and compared t...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/01.STR.0000160751.79241.a3

    authors: Sobesky J,Zaro Weber O,Lehnhardt FG,Hesselmann V,Neveling M,Jacobs A,Heiss WD

    更新日期:2005-05-01 00:00:00

  • Carbon Monoxide Preserves Circadian Rhythm to Reduce the Severity of Subarachnoid Hemorrhage in Mice.

    abstract:BACKGROUND AND PURPOSE:Subarachnoid hemorrhage (SAH) is associated with a temporal pattern of stroke incidence. We hypothesized that natural oscillations in gene expression controlling circadian rhythm affect the severity of neuronal injury. We moreover predict that heme oxygenase-1 (HO-1/Hmox1) and its product carbon ...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/STROKEAHA.116.016165

    authors: Schallner N,Lieberum JL,Gallo D,LeBlanc RH 3rd,Fuller PM,Hanafy KA,Otterbein LE

    更新日期:2017-09-01 00:00:00

  • Alberta Stroke Program Early CT Scale evaluation of multimodal computed tomography in predicting clinical outcomes of stroke patients treated with aspiration thrombectomy.

    abstract:BACKGROUND AND PURPOSE:Patient selection is crucial in the endovascular treatment of acute ischemic stroke patients. Baseline computed tomographic (CT) images, evaluated with the Alberta Stroke Program Early CT Scale (ASPECTS), are considered significant predictors of outcome. In this study, we evaluated CT images and ...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/STROKEAHA.113.001068

    authors: Psychogios MN,Schramm P,Frölich AM,Kallenberg K,Wasser K,Reinhardt L,Kreusch AS,Jung K,Knauth M

    更新日期:2013-08-01 00:00:00

  • Topography and temporal evolution of hypoxic viable tissue identified by 18F-fluoromisonidazole positron emission tomography in humans after ischemic stroke.

    abstract:BACKGROUND AND PURPOSE:We sought to characterize the spatial and temporal evolution of human cerebral infarction. Using a novel method of quantitatively mapping the distribution of hypoxic viable tissue identified by 18F-fluoromisonidazole (18F-FMISO) PET relative to the final infarct, we determined its evolution and s...

    journal_title:Stroke

    pub_type: 临床试验,杂志文章

    doi:10.1161/01.STR.0000094422.74023.FF

    authors: Markus R,Reutens DC,Kazui S,Read S,Wright P,Chambers BR,Sachinidis JI,Tochon-Danguy HJ,Donnan GA

    更新日期:2003-11-01 00:00:00

  • Muscle sympathetic nerve activity during cold pressor test in patients with cerebrovascular accidents.

    abstract:BACKGROUND AND PURPOSE:Autonomic dysfunction is frequently present in patients with cerebrovascular accidents (CVA). However, the pathophysiological mechanisms of these disorders are not clear. The purpose of the study was to assess the effects of CVA on the autonomic nervous system. METHODS:In eight male patients wit...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/01.str.29.3.607

    authors: Mizushima T,Tajima F,Nakamura T,Yamamoto M,Lee KH,Ogata H

    更新日期:1998-03-01 00:00:00

  • Transesophageal two-dimensional echocardiography in young patients with cerebral ischemic events.

    abstract::Using transesophageal echocardiography, cardiac structures can be imaged with high resolution. The aim of our study was to evaluate whether transesophageal echocardiography is superior in detecting mitral valve prolapse and other cardiac abnormalities compared with transthoracic echocardiography in an age-matched cont...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/01.str.19.3.345

    authors: Zenker G,Erbel R,Krämer G,Mohr-Kahaly S,Drexler M,Harnoncourt K,Meyer J

    更新日期:1988-03-01 00:00:00

  • Optimal depth and duration of mild hypothermia in a focal model of transient cerebral ischemia: effects on neurologic outcome, infarct size, apoptosis, and inflammation.

    abstract:BACKGROUND AND PURPOSE:Mild hypothermia is possibly the single most effective method of cerebroprotection developed to date. However, many questions regarding mild hypothermia remain to be addressed before its potential implementation in the treatment of human stroke. Here we report the results of 2 studies designed to...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/01.str.29.10.2171

    authors: Maier CM,Ahern Kv,Cheng ML,Lee JE,Yenari MA,Steinberg GK

    更新日期:1998-10-01 00:00:00

  • Physical activity and stroke incidence: the Harvard Alumni Health Study.

    abstract:BACKGROUND AND PURPOSE:Physiologically, it appears plausible for physical activity to decrease stroke risk; however, epidemiological studies have produced mixed findings. Furthermore, few studies have examined specific kinds and intensities of activities. The purpose of this study was to examine the association between...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/01.str.29.10.2049

    authors: Lee IM,Paffenbarger RS Jr

    更新日期:1998-10-01 00:00:00

  • Predicting improvement in gait after stroke: a longitudinal prospective study.

    abstract:BACKGROUND AND PURPOSE:To study the longitudinal relationship of functional change in walking ability and change in time-dependent covariates and to develop a multivariate regression model to predict longitudinal change of walking ability. METHODS:A total of 101 acute stroke patients with first-ever ischemic middle ce...

    journal_title:Stroke

    pub_type: 杂志文章,随机对照试验

    doi:10.1161/01.STR.0000190839.29234.50

    authors: Kollen B,van de Port I,Lindeman E,Twisk J,Kwakkel G

    更新日期:2005-12-01 00:00:00

  • Artery-to-artery embolism from a thrombus formed in stenotic middle cerebral artery. Report of an autopsy case.

    abstract::The authors report an autopsy case with repeated transient ischemic attacks and subsequent strokes demonstrating evidence of artery-to-artery embolism from thrombi generated in the stenotic middle cerebral artery to the distal cortical branches. This case substantiates the existence of intracranial artery-to-artery em...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/01.str.18.3.680

    authors: Masuda J,Ogata J,Yutani C,Miyashita T,Yamaguchi T

    更新日期:1987-05-01 00:00:00

  • Effect of insular injury on autonomic functions in patients with ruptured middle cerebral artery aneurysms.

    abstract:BACKGROUND AND PURPOSE:Insular injuries are known to cause autonomic derangements. Patients with ruptured middle cerebral artery aneurysms frequently develop temporal hematomas (THs) in addition to subarachnoid hemorrhages, and those with TH may sustain autonomic derangements more frequently than those without TH. Hemi...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/STROKEAHA.113.003099

    authors: Inamasu J,Sugimoto K,Watanabe E,Kato Y,Hirose Y

    更新日期:2013-12-01 00:00:00

  • Validity of self-reported stroke : The Tromso Study.

    abstract:BACKGROUND AND PURPOSE:The aim of this study was to validate the diagnosis of self-reported stroke. METHODS:During 1994-1995, 27 159 people attended a population health survey in the community of Tromso, Norway, a response rate of 77%. A total of 418 attenders reported a history of stroke. In 1997, all individuals wit...

    journal_title:Stroke

    pub_type: 杂志文章

    doi:10.1161/01.str.31.7.1602

    authors: Engstad T,Bonaa KH,Viitanen M

    更新日期:2000-07-01 00:00:00