Abstract:
OBJECTIVE:Triple-negative breast cancer (TNBC) is one of the most common malignant, highly heterogeneous tumors in women. MicroRNAs (miRNAs), such as miR-200c, play an important role in various types of malignant cancer, including TNBC. However, the biological role of miRNA-200c in TNBC is not well understood. In this study, we investigated the mechanism of miR-200c in the growth of TNBC. METHODS:Reverse transcription quantitative polymerase chain reaction was used to detect the expression of miR-200c in TNBC tissues and TNBC cells. Cell Counting Kit-8 (CCK-8) assays, wound healing, and transwell assays were used to observe the effects of miR-200c on TNBC cell proliferation, migration, and invasion, respectively. The expression of epithelial-mesenchymal transition (EMT) markers were detected by Western blotting. Dual luciferase reporter assays were used to test whether ZEB2 is a novel target of miR-200c. RESULT:Our results show that ZEB2 is a novel target of miR-200c and that ZEB2 mediates the metastasis of triple-negative breast cancer via EMT. CONCLUSION:miR-200c attenuates TNBC cell invasion and EMT by targeting ZEB2. Our data therefore suggest that miR-200c may be used to develop novel early-stage diagnostic and therapeutic strategies for TNBC.
journal_name
Ann Clin Lab Scijournal_title
Annals of clinical and laboratory scienceauthors
Chen H,Li Z,Zhang L,Zhang L,Zhang Y,Wang Y,Xu M,Zhong Qsubject
Has Abstractpub_date
2020-07-01 00:00:00pages
519-527issue
4eissn
0091-7370issn
1550-8080pii
50/4/519journal_volume
50pub_type
杂志文章abstract::Iron accumulation, especially that of free oxidized ferrous iron, has been shown to induce tissue oxidative damage and contribute to brain aging and the development of neurodegenerative disease. Here we examine whether sex and advanced age affect the expression of iron-related molecules that participate in regulating ...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2012-04-01 00:00:00
abstract::Nitric oxide is generated from L-arginine by the action of nitric oxide synthase, an enzyme encoded by three different genes. Nitric oxide is involved in an expanding number of phenomena. This involvement may be documented by direct detection using spectrophotometric or electrochemical methods or more often by indirec...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章,评审
doi:
更新日期:1996-11-01 00:00:00
abstract::The purpose of this study was to evaluate immunoassay methods for the measurement of serum cardiac creatine kinase isoenzyme (CK-MB) with respect to sensitivity and specificity. The CK-MB electrophoretic assay (Helena Laboratories) was used as the reference. Two principles of immunoassay were included in the evaluatio...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1994-03-01 00:00:00
abstract::The detection of oligoclonal bands of IgG in cerebrospinal fluid by isoelectric focusing is a useful aid in the diagnosis of multiple sclerosis. At present, this test is performed on polyacrylamide gels which are technically very difficult to prepare and are not suitable for the routine laboratory. A technique is desc...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1984-03-01 00:00:00
abstract::More than 300 million routine clinical analyses are performed annually in the United States. Methods for routine clinical urine examination, including detection of bacteriuria, are briefly reviewed. Prospects of some newer, better techniques to carry out such analyses are introduced. A preliminary report is presented ...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章,评审
doi:
更新日期:1999-07-01 00:00:00
abstract::A rapid homogeneous enzyme immunoassay for theophylline has been adapted to a centrifugal analyzer. The sample volume used was 10 microliter and sera from as many as 28 patients could be measured in 180 seconds. The assay temperature was 30 degrees and the absorbance rates were measured at 340 nm. Within-run and run-t...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1980-07-01 00:00:00
abstract::Lung cancer is the leading cause of cancer-related death worldwide. Lung adenocarcinoma (AC) cases account for approximately 40% of lung cancers. Early diagnosis can reduce mortality, improve prognosis, prolong survival, and improve quality of life. Specific and stable biomarkers for early diagnosis of AC are still la...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2018-11-01 00:00:00
abstract::The aim of this study was to assess the efficacy and pharmacokinetic profiles of gentamicin, vancomycin, and levofloxacin in a rat air pouch model, in which Staphylococcus aureus (ATCC 25293) was used as the test organism. Antibiotic treatments (i.p.) were started 1 hour after bacterial inoculation and continued for 5...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2012-01-01 00:00:00
abstract::Within the next 20-25 years, 20% of the U.S. population is expected to be 65 years old or older. Unless significant changes are made (ie, increased research, improved treatment, and promotion of health and disease prevention), medical costs will continue to rise but with fewer citizens to finance them. However, this n...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章,评审
doi:
更新日期:2000-01-01 00:00:00
abstract::Increased lipid peroxidation (LP) occurs in the presence of various transition, metal ions, including copper and iron, as well as various heme moieties. In addition, irradiation significantly increases LP. The degree of LP, as determined by the liquid chromatographic measurement of plasma malondialdehyde (MDA), can be...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract:OBJECTIVE:This study aims to investigate the effect of different concentrations of docosahexaenoic acid (DHA) on proliferation and apoptosis in HepG2 cell lines, and to research the possible molecular mechanisms. METHODS:DHA concentration was 0 g/mL in the negative control group, and 15, 30, 45, 60 and 75 ug/mL, respe...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2019-01-01 00:00:00
abstract::Several studies have provided reference ranges for the concentration of serum transferrin receptor (sTfR) in various white populations, but there is a dearth of relevant reference sTfR data in non-whites. The aim of this investigation was to establish sTfR reference ranges and mean values for a healthy non-white Arab ...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::Primary adenocarcinoma of the fallopian tube is a rare malignant neoplasm of the female reproductive system. Unusual patterns of metastasis may be observed. Report is made of a patient with recurrent adenocarcinoma of the fallopian tube, which presented as leptomeningeal carcinomatosis, as a first site of distant meta...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1996-03-01 00:00:00
abstract::Gain-of-function mutations of the CKIT gene have been reported to specifically occur in core-binding factor (CBF) acute myeloid leukemia (AML) with a poor prognostic implication. Here we report a case of therapy-related AML with t(9;11)(p22;q23) who had CKIT mutation. A 48-year-old woman with breast cancer received pa...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2011-04-01 00:00:00
abstract::Von Willebrand factor is a complex protein which is important in several ways for normal hemostasis. Von Willebrand's disease results when there is either a quantitative or qualitative disorder of von Willebrand factor. In this review, the structure and function of von Willebrand factor are discussed. Additionally, th...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章,评审
doi:
更新日期:1989-05-01 00:00:00
abstract::Blood lymphocyte distributions, serum immunoglobulin and cytokine levels, and serum IgE and IgG anti-varicella zoster virus (VZV) levels were measured in an atopic girl (age 15 yr) who developed shingles 10 yr after infection with chicken pox. Before, during, and 5 months after the shingles episode, the child's distri...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::This study compared the assay results of HBV markers obtained with two automated immunoassay systems in serum samples with concomitant HBV antigens and antibodies. Eighty-nine samples of concomitant HBsAg and anti-HBs and 74 samples of concomitant HBeAg and anti-HBe were analyzed by the Abbott Architect i2000 and the ...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract::The most common ultrastructural features of endocrine tumors are reviewed. The diagnostic applications of the electron microscope are illustrated by citation of case histories. ...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1979-03-01 00:00:00
abstract::A simple determination of 5'-Nucleotidase in blood serum without deproteinization is described. The enzyme activity is distinguished from that of a nonspecific alkaline phosphatase by nickel inhibition and the inorganic phosphate released from adenosine monophosphate used as substrate is determined by a method previou...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1977-11-01 00:00:00
abstract::A brief historical review is presented of the development of bovine serum albumin as a standard for the determination of serum total protein. Bovine serum albumin is now available as a standard reference material from the National Bureau of Standards. Also reviewed is the present status of the immunoglobulin standards...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1978-05-01 00:00:00
abstract::Iron pill gastritis has been shown to be associated with superficial gastric erosion and deposition of iron in lamina propria and gastric antral glands. However, iron absorption in gastric parietal and chief cells is rare. We present a case of a 62-year-old man with iron deficiency anemia. His past medical history is ...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2017-05-01 00:00:00
abstract:BACKGROUND:Quality assurance of newly developed or manufactured blood grouping reagents with reagent red blood cells (RBCs) is crucial in the process of product approval by governmental agency. However, RBCs with rare blood group are not easily available in the fresh state. We investigated the feasibility of cryopreser...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2018-03-01 00:00:00
abstract:CONTEXT:Myotonic dystrophies (DMs) are a group of autosomal dominant neuromuscular disorders which are caused by large CTG/CCTG-repeat expansions in untranslated regions of DMPK/ZNF9 gene. The "phenotypic overlap" in DMs creates complication in distinguishing patients with DM1 from patients with DM2 and underscores the...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2015-10-01 00:00:00
abstract::The aim of the study was to determine if amniotic fluid cells of rats can be used to provide evidence of genotoxicity. In order to do that micronuclei formation was induced in rats during pregnancy after treatment with cyclophosphamide (CP), at different CP doses. On gestational day 19, we collected the amniotic fluid...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2018-03-01 00:00:00
abstract::Toll-like receptors (TLR) activation is thought to modulate the macrophage cholesterol efflux and contribute to the atherosclerosis progression; however, the precise pathophysiological mechanism remains unclear. We investigated the effects of TLR2- and TLR4-activation on the expression of the ATP-binding cassette (ABC...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2017-08-01 00:00:00
abstract::A retrospective study of acquired renal lesions in 42 renal transplants in children revealed a variety of histologic lesions. Although acute and chronic rejection changes were the most frequent, a number of metabolic, infectious, vascular, and other lesions were encountered. Renal biopsy allows rapid diagnosis of thes...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1981-07-01 00:00:00
abstract::Qualitative urinary screening procedures were converted to quantitative methods for urinary cystine and homocystine based on the reactions between these amino acids and cyanide-nitroprusside reagents. Cystine and homocystine are quantified by the measurement of absorbances at 521 and 524 nm, respectively. Cyanide-nitr...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract::We previously reported that proper dilution of sera that contain IgG monoclonal proteins (M-proteins) is necessary for accurate quantitation of serum albumin and M-protein concentrations separated by serum protein electrophoresis (SPE) using the Beckman PARAGON agarose electrophoresis system. We now report the signifi...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2003-04-01 00:00:00
abstract::Previous studies have suggested that there is a disproportionally higher risk of infection following traumatic brain injury (TBI). This predisposition to infection may be driven by a poorly understood, brain-specific response in the immune system after TBI. However, there is a lack of studies that have fully character...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2017-01-01 00:00:00
abstract::Trehalose 6,6' dimycolate (TDM), a mycobacterial glycolipid, induces granulomas and hemorrhagic toxic reactions when administered in oil but not as a suspension in saline. It was previously demonstrated by us that TDM forms highly structured layers at oil-water interfaces and then postulated that its toxicity derives ...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1993-07-01 00:00:00