Abstract:
:Lactic acid bacteria (LAB) are important microorganisms for the food industry due to their functional activity, as starters and potential probiotic strains. With that in mind, we explored the LAB diversity in raw buffalo milk, screening for novel potential probiotic strains. A total of 11 strains were identified by combination of MALDI-TOF and partial 16S rDNA sequencing and selected as potential probiotic candidates. Bacteria innocuity assessment was performed by determining antimicrobial susceptibility and the presence of virulence factors. Antagonism activity against Escherichia coli, Pseudomonas aeruginosa, Listeria monocytogenes and Staphylococcus aureus was assessed, as well as milk proteolytic activity and exopolysaccharides production. Seven strains were identified as innocuous and two of them, Lactobacillus rhamnosus LB1.5 and Lactobacillus paracasei LB6.4 were selected for further probiotic potential analyses. Both strains demonstrated adhesion ability to Caco-2 cells, coaggregated with S. aureus and E. coli and maintained cell viability after gastrointestinal simulation in vitro, suggesting their probiotic potential. Furthermore, the transcriptional response of Lact. rhamnosus LB1.5 and Lact. paracasei LB6.4 to in vitro acid stress was assessed by RT-qPCR targeting seven genes related to adhesion, aggregation, stress tolerance, DNA repair and central metabolism. The association between the transcriptional responses and the maintenance of cell viability after gastrointestinal simulation highlights the genetic ability as probiotic of the two selected strains. Finally, we have concluded that Lact. rhamnosus LB1.5 and Lact. paracasei LB6.4 are important probiotic candidates to further in vivo studies.
journal_name
Probiotics Antimicrob Proteinsjournal_title
Probiotics and antimicrobial proteinsauthors
Breyer GM,Arechavaleta NN,Siqueira FM,de Souza da Motta Adoi
10.1007/s12602-020-09700-4subject
Has Abstractpub_date
2020-08-23 00:00:00eissn
1867-1306issn
1867-1314pii
10.1007/s12602-020-09700-4pub_type
杂志文章abstract::Lactiplantibacillus plantarum subsp. plantarum 299v (L. plantarum 299v) is one of the most important probiotic strains in animal health, but the molecular mechanisms of how it exerts health benefits remain unclear. The purpose of this study was to explore the changes in miRNA expression profiles in the intestinal tiss...
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journal_title:Probiotics and antimicrobial proteins
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journal_title:Probiotics and antimicrobial proteins
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journal_title:Probiotics and antimicrobial proteins
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abstract::The original version of this article contained mistakes, and the authors would like to correct them. The correct details are given below. ...
journal_title:Probiotics and antimicrobial proteins
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journal_title:Probiotics and antimicrobial proteins
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journal_title:Probiotics and antimicrobial proteins
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journal_title:Probiotics and antimicrobial proteins
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journal_title:Probiotics and antimicrobial proteins
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journal_title:Probiotics and antimicrobial proteins
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journal_title:Probiotics and antimicrobial proteins
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journal_title:Probiotics and antimicrobial proteins
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journal_title:Probiotics and antimicrobial proteins
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journal_title:Probiotics and antimicrobial proteins
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abstract::The fate of dietary protein in the gut is determined by microbial and host digestion and utilization. Fermentation of proteins generates bioactive molecules that have wide-ranging health effects on the host. The type of protein can affect amino acid absorption, with animal proteins generally being more efficiently abs...
journal_title:Probiotics and antimicrobial proteins
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journal_title:Probiotics and antimicrobial proteins
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doi:10.1007/s12602-017-9302-x
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abstract::The aim of this work is to characterize the potential probiotic properties of 14 antifungal Lactobacillus strains isolated from traditional fermenting Moroccan green olives. The molecular identification of strains indicated that they are composed of five Lactobacillus brevis, two Lactobacillus pentosus, and seven Lact...
journal_title:Probiotics and antimicrobial proteins
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abstract::We studied as hydrogen peroxide, lactic acid, or surfactants from clinical isolates of vaginal lactobacilli and cell-free supernatants from probiotic strain LCR35 can influence on the sensitivity of opportunistic bacteria to antibiotics. We found that the most effective in increasing sensitivity to antibiotics were hy...
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