Per Artursson's Major Contributions to the Caco-2 Cell Literature in Pharmaceutical Sciences.


:This edition of the Journal of Pharmaceutical Sciences is dedicated to the wonderful career of Per Artursson from the Uppsala University. My Commentary focusses on Per's major contributions to the Caco-2 cell literature over the past 30 years. Two especially influential papers have been cited more than 1000 times out of a total citation count of almost 30,000 and a h-index of 93 (Google Scholar), making Per one of the most cited and influential Pharmaceutical scientists of his generation. The Caco-2 field to which Per contributed so many advances has informed the community on key areas including predictive drug fluxes across the intestine, metabolism by intestinal epithelia, the role of transporters during flux, enantiomer-selective flux, excipient interaction with tight junctions, and nanoparticle uptake by enterocytes. In this pioneering work, Per has been careful to emphasise that Caco-2 monolayers have limitations and are a model of the human small intestine where observations must be backed up with in vitro tissue and in vivo work. Throughout, he has paid great attention to detail in methodology, as reflected by co-authorship of two Nature Protocols on Caco-2 assays. The article briefly assesses some of the most important milestones in Per's published Caco-2 research.


J Pharm Sci


Brayden DJ




Has Abstract


2021-01-01 00:00:00














  • Structural studies of commercial fat emulsions used in parenteral nutrition.

    abstract::In this paper, we demonstrate that by employing a combination of sedimentation field-flow fractionation (sedFFF) and other characterization techniques, such as photon correlation spectroscopy (PCS) and freeze-fracture electron microscopy (EM), it is possible to show that commercial fat emulsions of similar overall che...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Li J,Caldwell KD

    更新日期:1994-11-01 00:00:00

  • Pectin-gelatin complex coacervates II: Effect of microencapsulated sulfamerazine on size, morphology, recovery, and extraction of water-dispersible microglobules.

    abstract::Spherical medicated microglobules were prepared by complex coacervation of Type A gelatin with pectin, having nominal diameters of 5, 10, and 25 micron and containing 37.3, 44.9, and 45.2% (w/w) sulfamerazine, respectively. They were recovered as water-insoluble powders and were spontaneously revertible to highly disp...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: McMullen JN,Newton DW,Becker CH

    更新日期:1984-12-01 00:00:00

  • The Organic Anion-Transporting Peptide 2B1 Is Localized in the Basolateral Membrane of the Human Jejunum and Caco-2 Monolayers.

    abstract::The organic anion-transporting polypeptide (OATP) 2B1 which is ubiquitously expressed in the human body is assumed to play an important role in the cellular uptake of many drugs. Although the expression and function of this solute carrier transporter is well characterized in the human liver and other tissues, little i...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Keiser M,Kaltheuner L,Wildberg C,Müller J,Grube M,Partecke LI,Heidecke CD,Oswald S

    更新日期:2017-09-01 00:00:00

  • Simultaneous GLC determination of methyl salicylate and menthol in a topical analgesic formulation.

    abstract::A simultaneous GLC determination for methyl salicylate and menthol in a topical analgesic formulation is reported. n-Nonadecane is used as the internal standard. The method is rapid, accurate, precise, and selective in the presence of salicylic acid and ethyl salicylate. ...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Sapio JP,Sethachutkul K,Moody JE

    更新日期:1979-04-01 00:00:00

  • Development of a Stable Liquid Formulation for Live Attenuated Influenza Vaccine.

    abstract::Live attenuated influenza vaccine (LAIV) is considered one of the most effective vaccines and can be manufactured quickly and inexpensively to counter seasonal or pandemic influenza. Lyophilization is widely used in vaccine production. However, it requires a longer production cycle and large-scale equipment, thus posi...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Sun Y,Shen Z,Zhang C,Yi Y,Zhu K,Xu F,Kong W

    更新日期:2019-07-01 00:00:00

  • Determination of water-soluble acid distribution in poly(lactide-co-glycolide).

    abstract::Determination of the kinetics of water-soluble degradation products inside poly(lactide-co-glycolide) (PLGA) delivery systems during polymer degradation is important to evaluate the polymer microclimate conditions, particularly microclimate pH changes for optimization of encapsulated drug stability. A pre-derivatizati...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Ding AG,Schwendeman SP

    更新日期:2004-02-01 00:00:00

  • Indexing powder patterns in physical form screening: instrumentation and data quality.

    abstract::Two multisample laboratory powder diffractometers have been evaluated for the purpose of pattern indexing in the context of physical form screening. Both diffractometers utilise foil transmission geometry, primary monochromated radiation, and a position-sensitive detector. Data collected from six compounds (sotalol hy...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Florence AJ,Baumgartner B,Weston C,Shankland N,Kennedy AR,Shankland K,David WI

    更新日期:2003-09-01 00:00:00

  • Effect of individual Fc methionine oxidation on FcRn binding: Met252 oxidation impairs FcRn binding more profoundly than Met428 oxidation.

    abstract::The long serum half-lives of mAbs are conferred by pH-dependent binding of IgG-Fc to the neonatal Fc receptor (FcRn). The Fc region of human IgG1 has three conserved methionine residues, Met252, Met358, and Met428. Recent studies showed oxidation of these Met residues impairs FcRn binding and consequently affects phar...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Gao X,Ji JA,Veeravalli K,Wang YJ,Zhang T,Mcgreevy W,Zheng K,Kelley RF,Laird MW,Liu J,Cromwell M

    更新日期:2015-02-01 00:00:00

  • Freezing of Biologicals Revisited: Scale, Stability, Excipients, and Degradation Stresses.

    abstract::Although many biotech products are successfully stored in the frozen state, there are cases of degradation of biologicals during freeze storage. These examples are discussed in the Perspective to emphasize the fact that stability of frozen biologicals should not be taken for granted. Frozen-state degradation (predomin...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章,评审


    authors: Authelin JR,Rodrigues MA,Tchessalov S,Singh SK,McCoy T,Wang S,Shalaev E

    更新日期:2020-01-01 00:00:00

  • Solasodine stability under conditions of saponin hydrolysis.

    abstract::The extent of formation of solasodiene from solasodine upon treatment with hydrochloric acid under various conditions was determined. The diene formed was assayed by using the characteristic extinction at 236 nm. Diene formation was effected by the acid concentration and the boiling point of the solvent used. Optimal ...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Segal R,Breuer A,Milo-Goldzweig I

    更新日期:1978-08-01 00:00:00

  • The formation of oxytocin dimers is suppressed by the zinc-aspartate-oxytocin complex.

    abstract::The aim of this study was to investigate the effect of divalent metal ions (Ca, Mg(2+) , and Zn(2+) ) on the stability of oxytocin in aspartate buffer (pH 4.5) and to determine their interaction with the peptide in aqueous solution. Reversed-phase high-performance liquid chromatography and high-performance size-exclus...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Avanti C,Hinrichs WLJ,Casini A,Eissens AC,Van Dam A,Kedrov A,Driessen AJM,Frijlink HW,Permentier HP

    更新日期:2013-06-01 00:00:00

  • Comparison of analytical methods for residual ethylene oxide analysis.

    abstract::A round-robin study compared four methods of residual ethylene oxide analysis. Results from the six participating laboratories, along with a statistical treatment of the data, are presented. ...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Romano SJ,Renner JA

    更新日期:1975-08-01 00:00:00

  • PEGylation as a tool for the biomedical engineering of surface modified microparticles.

    abstract::Microparticles are of considerable interest for drug delivery, vaccination and diagnostic imaging. In order to obtain microparticles with long circulation times, or to provide the prerequisite for tissue specific targeting through decoration with suitable ligands, their surfaces need to be modified such that they beco...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章,评审


    authors: Wattendorf U,Merkle HP

    更新日期:2008-11-01 00:00:00

  • Quantitation of the area of overlap between second-derivative amide I infrared spectra to determine the structural similarity of a protein in different states.

    abstract::Maintaining a native-like structure of protein pharmaceuticals during lyophilization is an important aspect of formulation. Infrared spectroscopy can be used to evaluate the effectiveness of formulations in protecting the secondary structural integrity of proteins in the dried solid. This necessitates making quantitat...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Kendrick BS,Dong A,Allison SD,Manning MC,Carpenter JF

    更新日期:1996-02-01 00:00:00

  • Synthesis and bioevaluation of a series of fatty acid esters of p-[N,N-bis(2-chloroethyl)amino]phenol.

    abstract::A series of even numbered fatty acid esters (C2-C18) of p-[N,N-bis(2-chloroethyl)amino]phenol were synthesized and evaluated as to acute toxicity as well as effectiveness against L-1210 mouse leukemia. The acetate through the decanoate derivatives demonstrated toxicity between 2 and 3 times that of phenol mustard in H...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Wynn JE,Caldwell ML,Robinson JR,Beamer RL,Bauguess CT

    更新日期:1982-07-01 00:00:00

  • Bioavailability of sulfadiazine in rabbits using tablets prepared by direct compression and fluidized-bed granulation.

    abstract::Experimental sulfadiazine tablets prepared by direct compression, using a commercially available direct compression tablet mass, were compared with experimental sulfadiazine tablets prepared by fluidized-bed granulation and commercially available sulfadiazine tablets USP. The values for friability and the time require...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Ritschel WA,Erni W

    更新日期:1977-10-01 00:00:00

  • Synergistic effect of iontophoresis and a series of fatty acids on LHRH permeability through porcine skin.

    abstract::The effect of chemical penetration enhancers (e.g., fatty acids) in combination with iontophoresis was examined on the in vitro permeability of luteinizing hormone releasing hormone (LHRH) through porcine skin. Porcine epidermis was pretreated with either ethanol (EtOH) or 10% fatty acid/EtOH. The permeability coeffic...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Bhatia KS,Singh J

    更新日期:1998-04-01 00:00:00

  • Quantitative TLC determination of propranolol in human plasma.

    abstract::A spectrodensitometric assay was developed for propranolol based on measurement of the absorbance of the drug on silica gel plates irradiated at 822 nm. Quantities as low as 0.10 microgram can be detected, and a linear relationship was obtained between 0.010 and 0.400 microgram. The percent recovery from plasma spiked...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Hadzija BW,Mattocks AM

    更新日期:1978-09-01 00:00:00

  • Dissolution studies with a multichannel continuous-flow apparatus.

    abstract::A multichannel continuous-flow apparatus for dissolution rate measurements is described. Typical data are presented to demonstrate its utility for studies with bulk drug powders as well as with tablets and capsules without any change of setup. Procedures are given for the preparation of powder samples for dissolution ...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Cakiryildiz C,Mehta PJ,Rahmen W,Schoenleber D

    更新日期:1975-10-01 00:00:00

  • Disposition of 8-methyl-8-azabicyclo[3,2,1]octan-3-yl 3,5-dichlorobenzoate, a potent 5-hydroxytryptamine antagonist, and two metabolites in dogs and monkeys.

    abstract::The disposition of 8-methyl-8-azabicyclo[3,2,1]octan-3-yl 3,5-dichlorobenzoate (MDL 72,222; 1), a potent 5-hydroxytryptamine antagonist, and its N-demethylated and N-oxide metabolites was studied in dogs and monkeys. After single, intravenous doses of 1 at 5 mg/kg, the mean terminal half-lives of 1 in plasma were 2.6 ...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Cheng H,Larsen DL,Ragner JA,Sproles GD,Gordon WP

    更新日期:1992-04-01 00:00:00

  • Determination of nofedone in human serum by electron-capture GLC.

    abstract::An electron-capture GLC method to measure nofedone in human serum was developed. A homolog of nofedone was added to the serum as an internal standard before the sample was alkalinized with pH 9.5 phosphate buffer and extracted with ethylene dichloride containing 0.5% isopentyl alcohol. This organic phase was extracted...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Heusse D,Populaire P,Renard A,Pasquier P,Gregoire J

    更新日期:1980-05-01 00:00:00

  • Time-dependent changes in the activation of RhoA/ROCK and ERM/p-ERM in the increased expression of intestinal P-glycoprotein by repeated oral treatment with etoposide.

    abstract::Previously, we reported that repeated oral treatment with etoposide (ETP) increased ileal membrane localization of P-glycoprotein (P-gp) and that this was possibly mediated by increased expression of the ezrin/radixin/moesin (ERM)/phosphorylated ERM (p-ERM) via activation of RhoA/ROCK. These changes caused decreases i...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Kobori T,Harada S,Nakamoto K,Tokuyama S

    更新日期:2013-05-01 00:00:00

  • Introduction of solvent-accessible surface area in the calculation of the hydrophobicity parameter log P from an atomistic approach.

    abstract::A conventional atomistic approach of estimating molecular hydrophobicity (log P) was improved by taking into account the proximity effect of substituent groups as well as the importance of solute-solvent interaction in the partition phenomena. The new method reassigns atomic parameters when the molecule is fully expos...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Masuda T,Jikihara T,Nakamura K,Kimura A,Takagi T,Fujiwara H

    更新日期:1997-01-01 00:00:00

  • Characterization of glucose-mediated insulin release from implantable polymers.

    abstract::We characterized a glucose-sensitive, controlled-release insulin delivery system. Insulin release rates increased when glucose was perfused in the release media surrounding the matrix. The system was composed of solid, particulate insulin, incorporated into an ethylene-vinyl acetate copolymer (EVAc) matrix. Feedback c...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Brown LR,Edelman ER,Fischel-Ghodsian F,Langer R

    更新日期:1996-12-01 00:00:00

  • How do formulation and process parameters impact blend and unit dose uniformity? Further analysis of the product quality research institute blend uniformity working group industry survey.

    abstract::Responses from the second Product Quality Research Institute (PQRI) Blend Uniformity Working Group (BUWG) survey of industry have been reanalyzed to identify potential links between formulation and processing variables and the measured uniformity of blends and unit dosage forms. As expected, the variability of the ble...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Hancock BC,Garcia-Munoz S

    更新日期:2013-03-01 00:00:00

  • Synthesis and therapeutic testing of mono- and dialkyl esters of pentetic (diethylenetriaminepentaacetic) acid for decorporation of polymeric plutonium.

    abstract::The synthesis, characterization, and therapeutic evaluation of a series of partially esterified derivatives of pentetic (diethylenetriaminepentaacetic) acid are reported. These compounds were prepared in an attempt to promote increased decorporation of insoluble colloidal forms of plutonium, which are removed by pente...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Guilmette RA,Parks JE,Lindenbaum A

    更新日期:1979-02-01 00:00:00

  • Anticonvulsant activity of some 4-methoxy- and 4-chlorobenzanilides.

    abstract::A series of mono-, di-, and trimethylated derivatives of 4-chloro- and 4-methoxybenzanilide was synthesized and evaluated for anticonvulsant activity. This series was prepared in the course of studies designed to examine the relationship between anticonvulsant effects and benzamide structure. The compounds were tested...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Clark CR,McMillian CL

    更新日期:1990-03-01 00:00:00

  • Comparison of granule strength and tablet tensile strength.

    abstract::The granule strength (crushing load) of lactose granulated with 1-9% povidone was measured initially and at intervals during a 1-year period. The granule strengths of dibasic calcium phosphate dihydrate granulated with various concentrations of starch and povidone were measured. The axial and radial tensile strengths ...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Jarosz PJ,Parrott EL

    更新日期:1983-05-01 00:00:00

  • Development of a Carrier-Free Dry Powder Ofloxacin Formulation With Enhanced Aerosolization Properties.

    abstract::Tuberculosis (TB) is a serious infectious disease that affects more than new 10 million patients each year. Many of these cases are resistant to first-line drugs so second-line ones, like fluoroquinolones, need to be incorporated into the therapeutic. Ofloxacin (OF) is a fluoroquinolone which demonstrates high antibio...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Ceschan NE,Rosas MD,Olivera ME,Dugour AV,Figueroa JM,Bucalá V,Ramírez-Rigo MV

    更新日期:2020-09-01 00:00:00

  • Analytical methods for the determination of sulindac and metabolites in plasma, urine, bile, and gastric fluid by liquid chromatography using ultraviolet detection.

    abstract::A high-performance liquid chromatographic method using a linear elution gradient has been developed for the analysis of sulindac, sulindac sulfone, and sulindac sulfide in plasma, urine, bile, and gastric fluid. The methodology uses reverse-phase, radial compression chromatography with gradient elution, and UV detecti...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Musson DG,Vincek WC,Constanzer ML,Detty TE

    更新日期:1984-09-01 00:00:00