当前位置： SCI文献检索 > AMINO ACIDS期刊下所有文献 > Antimicrobial activity and membrane-active mechanism of tryptophan zipper-like β-hairpin antimicrobial peptides.

Antimicrobial activity and membrane-active mechanism of tryptophan zipper-like β-hairpin antimicrobial peptides.

Abstract：

:Antimicrobial peptides (AMPs) with amphipathic β-hairpin structures have been demonstrated to possess potent antimicrobial activities and great cell selectivities. However, our understanding of β-hairpin antimicrobial peptides lags behind that of α-helices, mainly because it is difficult for short peptides to form robust β-hairpin structures. Tryptophan zipper (trpzip) peptides are among the most stable β-hairpin peptides known to fold spontaneously without requiring covalent disulfide constraint or metal binding. To develop model β-hairpin AMPs with small size and remarkable stability, a series of amphiphilic linear peptides were designed based on the trpzip motif. The sequence of designed peptides is (WK) n (D) PG(KW) n -NH2 (n = 1, 2, 3, 4, 5), and the antimicrobial activity and membrane interaction mechanism of the peptides were evaluated. The results showed that these peptides readily fold into β-hairpin structures in aqueous and membrane-mimicking environments and exhibit broad-spectrum antimicrobial activities against both gram-positive and gram-negative bacteria. The antibacterial potency of the peptides initially increased and then decreased with increasing chain length. WK3, a 14-residue peptide, displayed excellent antimicrobial activity with minimal hemolytic activity and cytotoxicity, suggesting that it possesses great cell selectivity. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), fluorescence spectroscopy, and flow cytometry indicated that representative peptides WK3 and WK4 exert their activities by permeabilizing the microbial membrane and damaging cell membrane integrity. This study reveals the application potential of the designed peptides as promising antimicrobial agents for the control of infectious diseases, and it also provides new insights into the design and optimization of highly stable β-hairpin AMPs with great antimicrobial activities and cell selectivities.

journal_name

Amino Acids

journal_title

Amino acids

authors

Xu L,Chou S,Wang J,Shao C,Li W,Zhu X,Shan A

doi

10.1007/s00726-015-2029-7

subject

Has Abstract

pub_date

2015-11-01 00:00:00

pages

2385-97

issue

11

eissn

0939-4451

issn

1438-2199

pii

10.1007/s00726-015-2029-7

journal_volume

47

pub_type

杂志文章

相关文献

AMINO ACIDS文献大全
  • Effect of taurine chloramine, the product of activated neutrophils, on the development of collagen-induced arthritis in DBA 1/J mice.

    abstract：:Taurine chloramine (TauCl), a product of neutrophil myeloperoxidase - halide system, formed by a reaction of taurine with HOCl, is known as an anti-microbial and anti-inflammatory long-lived oxidant. We previously reported that TauCl inhibits in vitro the production of proinflammatory cytokines (IL-6, IL-8) by RA syno...

    journal_title：Amino acids

    pub_type： 杂志文章

    doi：10.1007/s00726-002-0207-x

    authors： Kwaśny-Krochin B,Bobek M,Kontny E,Gluszko P,Biedroń R,Chain BM,Maśliński W,Marcinkiewicz J

    更新日期：2002-01-01 00:00:00

  • The role of dietary creatine.

    abstract：:The daily requirement of a 70-kg male for creatine is about 2 g; up to half of this may be obtained from a typical omnivorous diet, with the remainder being synthesized in the body Creatine is a carninutrient, which means that it is only available to adults via animal foodstuffs, principally skeletal muscle, or via su...

    journal_title：Amino acids

    pub_type： 杂志文章,评审

    doi：10.1007/s00726-016-2188-1

    authors： Brosnan ME,Brosnan JT

    更新日期：2016-08-01 00:00:00

  • Cell-type specificity of mGluR activation in striatal neuronal subtypes.

    abstract：:The effects of metabotropic glutamate receptor (mGluR) activation were studied in medium spiny neurons and large aspiny (LA) interneurons by means of electrophysiological and optical recordings. DCG-IV and L-SOP, agonists for group II and III mGluRs, respectively, produced a presynaptic inhibitory effect on corticostr...

    journal_title：Amino acids

    pub_type： 杂志文章

    doi：10.1007/s007260070040

    authors： Pisani A,Bernardi G,Bonsi P,Centonze D,Giacomini P,Calabresi P

    更新日期：2000-01-01 00:00:00

  • Protein-polyamine conjugates by transglutaminase 2 as potential markers for antineoplastic screening of natural compounds.

    abstract：:The role of post-translational modification of cell proteins with polyamines, a reaction catalyzed by a tissue tranglutaminase (TG, EC 2.3.2.13), in the induction of cell differentiation, represents an intriguing strategy to control cell proliferation and metastatic ability of different tumor cell lines. In this revie...

    journal_title：Amino acids

    pub_type： 杂志文章,评审

    doi：10.1007/s00726-008-0157-z

    authors： Lentini A,Provenzano B,Tabolacci C,Beninati S

    更新日期：2009-04-01 00:00:00

  • Hydration of amino acids: FTIR spectra and molecular dynamics studies.

    abstract：:The hydration of selected amino acids, alanine, glycine, proline, valine, isoleucine and phenylalanine, has been studied in aqueous solutions by means of FTIR spectra of HDO isotopically diluted in H2O. The difference spectra procedure and the chemometric method have been applied to remove the contribution of bulk wat...

    journal_title：Amino acids

    pub_type： 杂志文章

    doi：10.1007/s00726-015-2005-2

    authors： Panuszko A,Adamczak B,Czub J,Gojło E,Stangret J

    更新日期：2015-11-01 00:00:00

  • Synthesis of medicinally useful lipidicα-amino acids, 2-amino alcohols and diamines.

    abstract：:The lipidicα-amino acids (LAAs) are non-naturalα-amino acids with saturated or unsaturated long aliphatic side chains. LAAs and their derivatives (lipid mimetics) together with the lipidic peptides represent a class of compounds which combine structural features of lipids with those of amino acids and peptides. Racemi...

    journal_title：Amino acids

    pub_type： 杂志文章

    doi：10.1007/BF00807940

    authors： Kokotos G,Martin V,Constantinou-Kokotou V,Gibbons WA

    更新日期：1996-09-01 00:00:00

  • Amino acids and gut function.

    abstract：:The intestine is not only critical for the absorption of nutrients, but also interacts with a complex external milieu. Most foreign antigens enter the body through the digestive tract. Dietary amino acids are major fuels for the small intestinal mucosa, as well as important substrates for syntheses of intestinal prote...

    journal_title：Amino acids

    pub_type： 杂志文章,评审

    doi：10.1007/s00726-008-0152-4

    authors： Wang WW,Qiao SY,Li DF

    更新日期：2009-05-01 00:00:00

  • Physiological and therapeutic effects of carnosine on cardiometabolic risk and disease.

    abstract：:Obesity, type 2 diabetes (T2DM) and cardiovascular disease (CVD) are the most common preventable causes of morbidity and mortality worldwide. They represent major public health threat to our society. Increasing prevalence of obesity and T2DM contributes to escalating morbidity and mortality from CVD and stroke. Carnos...

    journal_title：Amino acids

    pub_type： 杂志文章,评审

    doi：10.1007/s00726-016-2208-1

    authors： Baye E,Ukropcova B,Ukropec J,Hipkiss A,Aldini G,de Courten B

    更新日期：2016-05-01 00:00:00

  • Structure-activity relationships in ultrashort cationic lipopeptides: the effects of amino acid ring constraint on antibacterial activity.

    abstract：:Taking a minimalistic approach in efforts to lower the cost for the development of new synthetic antimicrobial peptides, ultrashort cationic lipopeptides were designed to mimic the amphiphilic nature crucial for their activity but with only a very short peptide sequence ligated to a lipidic acid. Nine ultrashort catio...

    journal_title：Amino acids

    pub_type： 杂志文章

    doi：10.1007/s00726-014-1806-z

    authors： Domalaon R,Yang X,O'Neil J,Zhanel GG,Mookherjee N,Schweizer F

    更新日期：2014-11-01 00:00:00

  • In sickness and in health: the widespread application of creatine supplementation.

    abstract：:There is an extensive and still growing body of the literature supporting the efficacy of creatine (Cr) supplementation. In sports, creatine has been recognized as the most effective nutritional supplement in enhancing exercise tolerance, muscle strength and lean body mass. From a clinical perspective, the application...

    journal_title：Amino acids

    pub_type： 杂志文章,评审

    doi：10.1007/s00726-011-1132-7

    authors： Gualano B,Roschel H,Lancha AH Jr,Brightbill CE,Rawson ES

    更新日期：2012-08-01 00:00:00

  • Structure-based design, synthesis and anticancer effect of cyclic Smac-polyarginine peptides.

    abstract：:The second mitochondria-derived activator of caspase (Smac/DIABLO) is a pro-apoptotic protein that released from mitochondria into the cytosol when cells undergo apoptosis. Smac promotes caspase activation by binding the inhibitors of apoptosis proteins (IAP), particularly XIAP and eliminating their inhibitory activit...

    journal_title：Amino acids

    pub_type： 杂志文章

    doi：10.1007/s00726-018-2637-0

    authors： Khalily MP,Gerekçi S,Güleç EA,Özen C,Özçubukçu S

    更新日期：2018-11-01 00:00:00

  • The effects of oral taurine administration on behavior and hippocampal signal transduction in rats.

    abstract：:Taurine, 2-aminoethylsulfonic acid, is one of the most abundant amino acids in the brain. It has various important physiological functions as a neuromodulator and antioxidant. Taurine is expected to be involved in depression; however, knowledge regarding its function in relation to depression is limited. In this study...

    journal_title：Amino acids

    pub_type： 杂志文章

    doi：10.1007/s00726-012-1282-2

    authors： Iio W,Matsukawa N,Tsukahara T,Toyoda A

    更新日期：2012-11-01 00:00:00

  • Acetaminophen reduces the protein levels of high affinity amino acid permeases and causes tryptophan depletion.

    abstract：:In yeast, toxicity of acetaminophen (APAP), a frequently used analgesic and antipyretic drug, depends on ubiquitin-controlled processes. Previously, we showed a remarkable overlap in toxicity profiles between APAP and tyrosine, and a similarity with drugs like rapamycin and quinine, which induce degradation of the ami...

    journal_title：Amino acids

    pub_type： 杂志文章

    doi：10.1007/s00726-018-2613-8

    authors： Huseinovic A,Dekker SJ,Boogaard B,Vermeulen NPE,Kooter JM,Vos JC

    更新日期：2018-10-01 00:00:00

  • Effects of S-adenosylmethionine decarboxylase, polyamines, amino acids, and weak bases (amines and ammonia) on development and ribosomal RNA synthesis in Xenopus embryos.

    abstract：:We have been studying control mechanisms of gene expression in early embryogenesis in a South African clawed toad Xenopus laevis, especially during the period of midblastula transition (MBT), or the transition from the phase of active cell division (cleavage stage) to the phase of extensive morphogenesis (post-blastul...

    journal_title：Amino acids

    pub_type： 杂志文章,评审

    doi：10.1007/s00726-009-0403-z

    authors： Shiokawa K,Aso M,Kondo T,Takai J,Yoshida J,Mishina T,Fuchimukai K,Ogasawara T,Kariya T,Tashiro K,Igarashi K

    更新日期：2010-02-01 00:00:00

  • Comparative metabolomics charts the impact of genotype-dependent methionine accumulation in Arabidopsis thaliana.

    abstract：:Methionine (Met) is an essential amino acid for all organisms. In plants, Met also functions as a precursor of plant hormones, polyamines, and defense metabolites. The regulatory mechanism of Met biosynthesis is highly complex and, despite its great importance, remains unclear. To investigate how accumulation of Met i...

    journal_title：Amino acids

    pub_type： 杂志文章

    doi：10.1007/s00726-010-0562-y

    authors： Kusano M,Fukushima A,Redestig H,Kobayashi M,Otsuki H,Onouchi H,Naito S,Hirai MY,Saito K

    更新日期：2010-10-01 00:00:00

  • Enhanced osteoblast adhesion on transglutaminase 2-crosslinked fibronectin.

    abstract：:Fibronectin (FN) is a cell adhesion protein that binds integrins in a process also involving the protein-crosslinking enzyme transglutaminase 2 (TG2) as a co-receptor. The cell-adhesive property of TG2 has been linked to a complex formation with FN and to its ability to crosslink and polymerize FN on the cell surface....

    journal_title：Amino acids

    pub_type： 杂志文章

    doi：10.1007/s00726-008-0125-7

    authors： Forsprecher J,Wang Z,Nelea V,Kaartinen MT

    更新日期：2009-04-01 00:00:00

  • Recovery of pan-genotypic and genotype-specific amino acid alterations in chronic hepatitis C after viral clearance: transition at the crossroad of metabolism and immunity.

    abstract：:Recovery of amino acid (AA) metabolism and the associated clinical implications in chronic hepatitis C (CHC) patients with sustained virological response (SVR) following anti-hepatitis C virus (HCV) therapy remains elusive. A prospective cohort study was conducted on 222 CHC patients with SVR. Eighty-two age-matched m...

    journal_title：Amino acids

    pub_type： 杂志文章

    doi：10.1007/s00726-016-2360-7

    authors： Chang ML,Cheng ML,Chang SW,Tang HY,Chiu CT,Yeh CT,Shiao MS

    更新日期：2017-02-01 00:00:00

  • Up-regulation of HIF-1α is associated with neuroprotective effects of agmatine against rotenone-induced toxicity in differentiated SH-SY5Y cells.

    abstract：:Agmatine, a metabolite generated by arginine decarboxylation, has been reported as neuromodulator and neuroactive substance. Several findings suggest that agmatine displays neuroprotective effects in several models of neurodegenerative disorders, such as Parkinson's disease (PD). It has been hypothesized that biogenic...

    journal_title：Amino acids

    pub_type： 杂志文章

    doi：10.1007/s00726-019-02759-6

    authors： Ferlazzo N,Currò M,Giunta ML,Longo D,Rizzo V,Caccamo D,Ientile R

    更新日期：2020-02-01 00:00:00

  • Polyamine depletion inhibits etoposide-induced NF-kappaB activation in transformed mouse fibroblasts.

    abstract：:In a previous research, we have shown that adequate levels of polyamines are required in transformed mouse fibroblasts for the correlated activations of MAPK subtypes (ERK and JNK) and caspases induced by etoposide and leading to apoptosis. We report now that the treatment of fibroblasts with etoposide also elicited a...

    journal_title：Amino acids

    pub_type： 杂志文章

    doi：10.1007/s00726-004-0101-9

    authors： Tantini B,Pignatti C,Fattori M,Fiumana E,Facchini A,Stefanelli C,Caldarera CM,Pegg AE,Flamigni F

    更新日期：2004-10-01 00:00:00

  • Enhancing effect of macroporous adsorption resin on gamma-aminobutyric acid production by Enterococcus faecium in whole-cell biotransformation system.

    abstract：:Gamma-aminobutyric acid (GABA) biosynthesis depended to a great extent on the biotransformation characterization of glutamate decarboxylase (GAD) and process conditions. In this paper, the enhancing effect of D101 macroporous adsorption resin (MAR) on the GABA production was investigated based on the whole-cell biotra...

    journal_title：Amino acids

    pub_type： 杂志文章

    doi：10.1007/s00726-020-02850-3

    authors： Yang SY,Liu SM,Jiang M,Wang BS,Peng LH,Zeng C

    更新日期：2020-05-01 00:00:00

  • The effects of red bull energy drink on human performance and mood.

    abstract：:The effects of Red Bull Energy Drink, which includes taurine, glucuronolactone, and caffeine amongst the ingredients, were examined over 3 studies in a total of 36 volunteers. Assessments included psychomotor performance (reaction time, concentration, memory), subjective alertness and physical endurance. When compared...

    journal_title：Amino acids

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1007/s007260170021

    authors： Alford C,Cox H,Wescott R

    更新日期：2001-01-01 00:00:00

  • Synthesis and biological activity of oxytocin analogues containing unnatural amino acids in position 9: structure activity study.

    abstract：:We report the solid phase synthesis and some pharmacological properties of 24 oxytocin (OT) analogues. Basic modifications at position 9 (introduction of L- or D-beta-(2-thienyl)-alanine [L- or D-Thi], or L- or D-3-Pyridylalanine [L- or D-3-Pal]) were combined with D-tyrosine(OEthyl) [D-Tyr(Et)] or D-1-naphthylalanine...

    journal_title：Amino acids

    pub_type： 杂志文章

    doi：10.1007/s00726-009-0372-2

    authors： Magafa V,Borovicková L,Slaninová J,Cordopatis P

    更新日期：2010-05-01 00:00:00

  • Optimal and effective oral dose of taurine to prolong exercise performance in rat.

    abstract：:The aim of this study was to determine the effective and optimum dose of taurine for exercise performance and to maintain tissue taurine concentration. Rats received a respective daily dose of 0, 20, 100, and 500 mg/kg body weight of taurine (EC and ET-1, -2, -3 groups, respectively) for two weeks, and then, were subj...

    journal_title：Amino acids

    pub_type： 杂志文章

    doi：10.1007/s00726-004-0133-1

    authors： Miyazaki T,Matsuzaki Y,Ikegami T,Miyakawa S,Doy M,Tanaka N,Bouscarel B

    更新日期：2004-12-01 00:00:00

  • Rehydration during exercise prevents the increase of homocysteine concentrations.

    abstract：:This study aimed to assess the effect of rehydration during and after acute aerobic submaximal exercise on total homocysteine (tHcy) concentrations and related parameters in physically active adult males. Twenty trained males (29.4 ± 7.9 years old) completed four exercise tests: two without rehydration during exercise...

    journal_title：Amino acids

    pub_type： 杂志文章,随机对照试验

    doi：10.1007/s00726-018-2655-y

    authors： Maroto-Sánchez B,Lopez-Torres O,Valtueña J,Benito PJ,Palacios G,Díaz-Martínez ÁE,González-Lamuño D,Zinellu A,González-Gross M,Carru C

    更新日期：2019-02-01 00:00:00

  • Intramuscular adaptations to eccentric exercise and antioxidant supplementation.

    abstract：:Prophylactic supplementation of N-acetyl-cysteine (NAC) and epigallocatechin gallate (EGCG) was studied for physiological and cellular changes in skeletal muscle after eccentric muscle contractions. Thirty healthy, active males (20.0 +/- 1.8 years, 160 +/- 7.1 cm, 76.1 +/- 17.0 kg) ingested for 14 days either 1,800 mg...

    journal_title：Amino acids

    pub_type： 杂志文章

    doi：10.1007/s00726-009-0432-7

    authors： Kerksick CM,Kreider RB,Willoughby DS

    更新日期：2010-06-01 00:00:00

  • Protection from hypoxic injury in cultured hepatocytes by glycine, alanine, and serine.

    abstract：:Isolated hepatocytes from rat liver in primary culture rapidly lost viability under hypoxic conditions. In the presence of glycine, L-alanine or L-serine loss of viability under hypoxic conditions was greatly retarded. Glycine and L-serine already showed significant protection from hypoxic injury at a concentration of...

    journal_title：Amino acids

    pub_type： 杂志文章

    doi：10.1007/BF00808120

    authors： Brecht M,de Groot H

    更新日期：1994-02-01 00:00:00

  • Leucine supplementation does not affect protein turnover and impairs the beneficial effects of endurance training on glucose homeostasis in healthy mice.

    abstract：:Endurance exercise training as well as leucine supplementation modulates glucose homeostasis and protein turnover in mammals. Here, we analyze whether leucine supplementation alters the effects of endurance exercise on these parameters in healthy mice. Mice were distributed into sedentary (C) and exercise (T) groups. ...

    journal_title：Amino acids

    pub_type： 杂志文章

    doi：10.1007/s00726-014-1903-z

    authors： Costa Júnior JM,Rosa MR,Protzek AO,de Paula FM,Ferreira SM,Rezende LF,Vanzela EC,Zoppi CC,Silveira LR,Kettelhut IC,Boschero AC,de Oliveira CA,Carneiro EM

    更新日期：2015-04-01 00:00:00

  • Glutathione and its associated enzymes in peripheral blood cells in different stages of chronic renal insufficiency.

    abstract：:Reduced glutathione (GSH) levels and glutathione reductase (GR) and glutathione S-transferase (GST) activities were investigated in the erythrocytes and lymphocytes of non-dialyzed patients with varying degrees of chronic renal insufficiency, and also of patients on regular hemodialysis treatment. GSH, GR and GST leve...

    journal_title：Amino acids

    pub_type： 杂志文章

    doi：10.1007/BF00805943

    authors： Mimic-Oka J,Simic T,Djukanovic L,Stefanovski J,Ramic Z

    更新日期：1992-10-01 00:00:00

  • Microwave-induced synthesis of new optically active and soluble polyamides containing pendent 4-(2-phthalimidiylpropanoylamino)benzoylamino-groups.

    abstract：:An aromatic chiral diacid monomer, 5-[4-(2-phthalimidiylpropanoylamino)-benzoylamino]isophthalic acid was synthesized in five steps under conventional heating in high yield and purity. A series of soluble, thermally stable and optically active polyamides (PA)s containing pendent groups made of phthalimide, flexible L:...

    journal_title：Amino acids

    pub_type： 杂志文章

    doi：10.1007/s00726-008-0186-7

    authors： Mallakpour S,Rafiee Z

    更新日期：2009-10-01 00:00:00

  • N-acetylcysteine reduces inflammation in the small intestine by regulating redox, EGF and TLR4 signaling.

    abstract：:This study determined whether N-acetylcysteine (NAC) could affect intestinal redox status, proinflammatory cytokines, epidermal growth factor (EGF), EGF receptor (EGFR), Toll-like receptor-4 (TLR4), and aquaporin-8 in a lipopolysaccharide (LPS)-challenged piglet model. Eighteen piglets (35-day-old) were randomly alloc...

    journal_title：Amino acids

    pub_type： 杂志文章

    doi：10.1007/s00726-012-1295-x

    authors： Hou Y,Wang L,Yi D,Ding B,Yang Z,Li J,Chen X,Qiu Y,Wu G

    更新日期：2013-09-01 00:00:00