Human Rhinovirus Infection Enhances the Th2 Environment in Allergic and Non-allergic Patients with Chronic Rhinosinusitis.

Abstract:

Objectives:This study was conducted to determine whether patients with allergy might be more susceptible to human rhinovirus (HRV) infection and whether the effects of infection on the elicited immune responses are different in allergic and non-allergic patients with chronic rhinosinusitis (CRS). Methods:Uncinate process tissues were obtained from 61 chronic rhinosinusitis patients (of which, 39 had allergy and 22 did not) who were infected with HRV16 using an air-liquid interface organ culture system. The expression levels of programmed cell death-ligand (PD-L)1, PD-L2, intracellular adhesion molecule 1 (ICAM-1), IFN-γ, IL-4, IL-5, and IL-10 were evaluated in the infected nasal mucosa. Results:The HRV infection rates were not significantly different between allergy (74.4 %) and non-allergy (72.7 %) groups. In the allergy group, the expression of PD-L1 (P=0.013) and IL-10 (P=0.040) was significantly elevated in the HRV-infected tissues; there was a strong correlation between PDL1 and IL-10 (r= 0.868, P<0.001). In contrast, infected tissues from the non-allergy group displayed increased levels of IL-4 (P=0.039), IL-5 (P=0.023), and IFN-γ (P=0.031) as well as an increased IL-4/IFN-γ ratio after HRV infection (P=0.043). Conclusion:This study shows that, regardless of the presence of allergy, HRV infection rates are similar in the nasal mucosa of patients with CRS. HRV infection enhances the Th2 environment by modulating PD-L1 and PD-L2 expression levels in allergic mucosa and by increasing the IL-4/IFN-γ ratio in non-allergic mucosa.

authors

Ko YK,Zhang YL,Wee JH,Han DH,Kim HJ,Rhee CS

doi

10.21053/ceo.2020.00444

subject

Has Abstract

pub_date

2020-09-11 00:00:00

eissn

1976-8710

issn

2005-0720

pii

ceo.2020.00444

pub_type

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