Molecular and serological characterization of occult hepatitis B among blood donors in Maputo, Mozambique.

Abstract:

BACKGROUND:Occult hepatitis B virus (HBV) - characterized by the absence of detectable HBsAg in the presence of HBV DNA - represents a potential threat for blood safety. OBJECTIVES:This study was conducted with the aim to investigate the serological and molecular characterization of occult HBV infection (OBI) among blood donors in Mozambique. METHODS:1,502 blood donors were tested for HBsAg. All HBsAg-negative individuals were tested for HBV DNA. Antibodies against HBV core, surface and HBe antigen (anti-HBc, anti-HBs, HBeAg) were measured in HBV DNA positive individuals. FINDINGS:1435 serum samples were HBsAg negative and 16 positive for HBV DNA, 14 confirmed to have OBI, corresponding to a frequency of 0.98%. Of the 14 OBI infections identified, 13/14 (92.8%) were positive for anti-HBc, 4/14 (28.5%) for anti-HBs, and no samples were reactive for HBeAg. Of the 14 OBI cases, nine samples (64.2%) were sequenced for the S/P region. Eight samples (88.9%) belonged to genotype A1 and one (11.1%) to genotype E. One escape mutation (T123A) associated with OBI and various amino acid substitutions for genotype A1 and E were observed. MAIN CONCLUSIONS:Our results show the importance of using nucleic acid amplification test to detect occult hepatitis B infection in blood donors in Mozambique.

journal_name

Mem Inst Oswaldo Cruz

authors

Mabunda N,Zicai AF,Ismael N,Vubil A,Mello F,Blackard JT,Lago B,Duarte V,Moraes M,Lewis L,Jani I

doi

10.1590/0074-02760200006

subject

Has Abstract

pub_date

2020-09-23 00:00:00

pages

e200006

eissn

0074-0276

issn

1678-8060

pii

S0074-02762020000100339

journal_volume

115

pub_type

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