Abstract:
:As a novel enteropathogenic coronavirus, porcine deltacoronavirus (PDCoV) warrants further investigation. In this study, a Chinese PDCoV strain, designated CHN-HN-1601, was isolated from the faeces of a diarrhoeic piglet. After plaque purification, the genome was determined which shared 97.5%-99.5% nucleotide identities with 71 representative PDCoV strains available in the GenBank. The pathogenic properties of CHN-HN-1601 were evaluated using 5-day-old piglets. All inoculated piglets developed severe diarrhoea from 2 days post-infection (dpi) onwards. To our surprise, two periods of diarrhoea starting from 2 to 7 dpi and from 13 to 19 dpi were observed in affected piglets during the experiment. Faecal viral shedding of the inoculated piglets was detected by real-time RT-PCR, with viral shedding peaked at 4 and 16 dpi, respectively. At necropsy at 5 dpi, the main gross lesions included transparent, thin-walled and gas-distended intestines containing yellow watery contents. Further histopathological examinations, including haematoxylin and eosin staining, immunohistochemistry and RNAscope in situ hybridization, revealed that the virus infection caused severe villous atrophy of the small intestines, with PDCoV antigen and RNA mainly distributed in the cytoplasm of the villous epithelial cells of jejunum and ileum in piglets. The dynamic production of PDCoV-specific IgG and neutralizing antibodies in serum of the affected piglets was also assessed using a whole virus-based ELISA and an immunofluorescence assay-based neutralization test, respectively. Furthermore, a full-length cDNA infectious clone of CHN-HN-1601 was constructed using a bacterial artificial chromosome system. The rescued virus exhibited in vitro growth and pathogenic properties similar to the parental virus. Taken together, our study not only enriches the information of PDCoV, but also provides a useful reverse genetics platform for further pathogenesis exploration of the virus.
journal_name
Transbound Emerg Disjournal_title
Transboundary and emerging diseasesauthors
Zhou X,Zhou L,Zhang P,Ge X,Guo X,Han J,Zhang Y,Yang Hdoi
10.1111/tbed.13862subject
Has Abstractpub_date
2020-10-04 00:00:00eissn
1865-1674issn
1865-1682pub_type
杂志文章abstract::An 8-month-old crossbred ewe, normal upon physical examination, was humanely euthanized for tissue collection. After approximately 3 weeks in tissue culture, fungi began budding out of cells obtained from the choroid plexus. After an additional 3 weeks, budding was observed in kidney cell cultures and eventually in mo...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12062
更新日期:2014-12-01 00:00:00
abstract::In 2011, a human brucellosis case with severe clinical symptoms was reported at the University Clinic for Infectious Diseases in Prishtina, Kosovo. A trace-back investigation was conducted to find the source of human infection. A total of 49 blood samples and 15 corresponding milk samples from sheep and goats raised o...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12336
更新日期:2016-12-01 00:00:00
abstract::Animal tuberculosis remains a great source of socioeconomic and health concern worldwide. Its main causative agents, Mycobacterium bovis and Mycobacterium caprae, have been isolated from many different domestic and wild animals. Naturally, occurring tuberculosis is extremely rare in rabbits, and implication of M. capr...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13366
更新日期:2020-01-01 00:00:00
abstract::Numerous infectious diseases impacting livestock impose an important economic burden and in some cases also represent a threat to humans and are classified as zoonoses. Some zoonotic diseases are transmitted by vectors and, due to complex environmental and socio-economic factors, the distribution of many of these path...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13776
更新日期:2020-08-07 00:00:00
abstract::We report details of the first seven equine cases of confirmed West Nile neuroinvasive disease in Austria. The cases presented during summer and autumn of 2016 (n = 2), 2017 (n = 3) and 2018 (n = 2). All horses showed gait abnormalities and 6 of 7 horses exhibited fasciculations and/or tremors, and we provide video re...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13452
更新日期:2020-05-01 00:00:00
abstract::The raw case fatality rate (CFR, the reported number of COVID-19 deaths divided by the total number of cases) is an important indicator to quantify the severity or treatment efficacy. In many countries, the pandemic had experienced two waves to date. To our knowledge, no studies have compared the CFR between the two w...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13819
更新日期:2020-09-06 00:00:00
abstract::Since African swine fever (ASF) first appeared in the Caucasus region in 2007, it has spread rapidly and is now present in numerous European and Asian countries. In Europe, mainly wild boar populations are affected and pose a risk for domestic pigs. In Asia, domestic pigs are almost exclusively affected. An effective ...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13678
更新日期:2020-11-01 00:00:00
abstract::Since the first report of bovine digital dermatitis (DD) in 1974, there is a large body of the literature published; however, effective prevention and control of the disease remain elusive. Although many aspects of the pathogenesis of DD have been investigated, even some of the most basic questions such as the aetiolo...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章,评审
doi:10.1111/tbed.12729
更新日期:2018-05-01 00:00:00
abstract::Beginning in 2006, point infection control operations and aerial distribution of oral rabies vaccines along the US border were performed in Quebec, Canada, to control the potential spread of raccoon rabies. A benefit-cost analysis assessed the economic efficiency of this rabies control programme into the future. In th...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/j.1865-1682.2012.01351.x
更新日期:2013-08-01 00:00:00
abstract::A case-control study conducted during 2011 involved 90 randomly selected commercial layer farms infected with highly pathogenic avian influenza type A subtype H5N1 (HPAI) and 175 control farms randomly selected from within 5 km of infected farms. A questionnaire was designed to obtain information about potential risk ...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12071
更新日期:2014-12-01 00:00:00
abstract::Bovine viral diarrhoea virus (BVDV) infection in cattle can result in growth retardation, reduced milk production, reproductive disorders and death. Persistently infected animals are the primary source of infection. In Hokkaido, Japan, all cattle entering shared pastures in summer are vaccinated before movement for di...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12693
更新日期:2018-02-01 00:00:00
abstract::Highly pathogenic avian influenza (HPAI) viruses continue to threaten smallholder poultry producers in several South-east Asian countries, including Vietnam. In particular, the free-grazing duck system has been repeatedly highlighted as a major risk factor for HPAI outbreaks. Free-grazing ducks, which scavenge on rice...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12697
更新日期:2018-04-01 00:00:00
abstract::Psittacine beak and feather disease (PBFD) is a common disease in psittacine bird that caused by beak and feather disease virus (BFDV). BFDV is widely spread and threatening psittacine birds worldwide. However, the BFDV infection in China remains largely unknown. In this study, a surveillance study of BFDV was conduct...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13274
更新日期:2019-09-01 00:00:00
abstract::Lumpy skin disease (LSD) is an economically important cattle disease. The disease is endemic in many African countries, but outbreaks have also been reported in Madagascar and the Middle East. The aim of this study was to investigate the potential role of ixodid (hard) ticks in the transmission of the disease. Cattle ...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/j.1865-1682.2010.01184.x
更新日期:2011-04-01 00:00:00
abstract::Quantitative real-time polymerase chain reaction was used to characterize the growth of Rickettsia slovaca, a tick-borne pathogen transmitted by Dermacentor reticulatus and D. marginatus ticks, in static (L929 and Vero cells) and dynamic (D. marginatus and Ixodes ricinus ticks) cultivation systems. The highest points ...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/j.1865-1682.2010.01104.x
更新日期:2010-04-01 00:00:00
abstract::In April 2012, highly pathogenic avian influenza virus of the H5N1 subtype (HPAIV H5N1) emerged in poultry layers in Ningxia. A retrospective case-control study was conducted to identify possible risk factors associated with the emergence of H5N1 infection and describe and quantify the spatial variation in H5N1 infect...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12433
更新日期:2017-06-01 00:00:00
abstract::European starlings (Sturnus vulgaris), house sparrows (Passer domesticus) and rock pigeons (Columba livia) are all wild birds commonly found in large numbers in and around human dwellings and domestic livestock operations. This study evaluated the susceptibility of these species to three strains of highly pathogenic a...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13147
更新日期:2019-05-01 00:00:00
abstract::Onchocerca lupi is a recently recognized threat for the health of animals and humans in European, American, African and Middle Eastern countries. We describe a case of imported O. lupi infection in Italy and report the lifespan of this parasite in a non-endemic area, to advocate increased awareness of the veterinary c...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12842
更新日期:2018-08-01 00:00:00
abstract::Data from affected lumpy skin disease (LSD) locations between July 2012 and September 2018 in the Balkans, Caucasus, and Middle East were retrieved from FAO's Global Animal Disease Information System (EMPRES-i) from the European Commission's Animal Disease Notification System (ADNS) and completed with data from the of...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13090
更新日期:2019-03-01 00:00:00
abstract::Brucella melitensis is a serious public health threat, with human infection exhibiting acute febrile illness and chronic health problems. The present study investigated the genetic diversity and epidemiological links of the important zoonotic bacterium B. melitensis in Egypt using multilocus variable-number tandem rep...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13581
更新日期:2020-11-01 00:00:00
abstract::Mycoplasma hyopneumoniae (M. hyopneumoniae) is the aetiologic agent of enzootic pneumonia in swine, a prevalent chronic respiratory disease worldwide. Mycoplasma hyopneumoniae is a small, self-replicating microorganism that possesses several characteristics allowing for limited biosynthetic abilities, resulting in the...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章,评审
doi:10.1111/tbed.13233
更新日期:2019-09-01 00:00:00
abstract::Avian influenza H7N9 viruses have caused five epidemic waves of human infections since the first human cases were reported in 2013. In 2016, the initial low pathogenic avian influenza (LPAI) H7N9 viruses became highly pathogenic, acquiring multi-basic amino acids at the haemagglutinin cleavage site. These highly patho...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13398
更新日期:2020-03-01 00:00:00
abstract::Piscine orthoreoviruses (PRVs) are emerging pathogens causing circulatory disorders in salmonids. PRV-1 is the etiological cause of heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar), characterized by epicarditis, inflammation and necrosis of the myocardium, myositis and necrosis of ...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13018
更新日期:2019-01-01 00:00:00
abstract::We use swine shipping data from Manitoba to construct one-mode dynamic contact networks of swine locations and two-mode location-to-truck networks at four time scales: daily, weekly, monthly and for the entire two-year study period. We provide measures of graph evolution and graph characterization for each, useful in ...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13220
更新日期:2019-09-01 00:00:00
abstract::Many aspects of the porcine reproductive and respiratory syndrome virus (PRRSV) between-farm transmission dynamics have been investigated, but uncertainty remains about the significance of farm type and different transmission routes on PRRSV spread. We developed a stochastic epidemiological model calibrated on weekly ...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.14007
更新日期:2021-01-27 00:00:00
abstract::Swine influenza viruses (SIVs) are important not only for pig farming, but also for public health. In fact, pandemic A(H1N1) 2009 viruses [A(H1N1)pdm09] were derived from SIVs. Therefore, timely characterization of locally circulating SIVs is necessary for understanding the global status of SIVs. To genetically charac...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12887
更新日期:2018-12-01 00:00:00
abstract::Outbreaks of highly pathogenic avian influenza (HPAI) have been reported worldwide. Wild waterfowl play a major role in the maintenance and transmission of HPAI. Highly pathogenic avian influenza subtype H5N6 and H5N8 viruses simultaneously emerged in South Korea. In this study, the comparative pathogenicity and infec...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12790
更新日期:2018-06-01 00:00:00
abstract::H5N1, a highly pathogenic avian influenza (H5N1 HPAI), is an endemic disease that is significant for public health in Egypt. Live bird markets (LBMs) are widespread in Egypt and play an important role in HPAI disease dynamics. The aim of the study was to evaluate the H5N1 HPAI prevalence in representative LBMs from 20...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12440
更新日期:2017-06-01 00:00:00
abstract::The time needed to wean porcine reproductive and respiratory syndrome (PRRS) virus negative pigs consistently from a breeding herd after an outbreak is referred to as time-to-stability (TTS). TTS is an important measure to plan herd closure as well as to manage economic expectations. Weekly PRRS incidence data from 82...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.13091
更新日期:2019-03-01 00:00:00
abstract::African swine fever (ASF) re-entered in Europe in 2007 by Georgia rapidly affecting neighbouring countries. Since then, ASF has caused severe problems to the Russian Federation (RF) and spread to Northern and Western regions, including Ukraine (2012 and 2014) and Belarus (2013). At the beginning of 2014, dead wild boa...
journal_title:Transboundary and emerging diseases
pub_type: 杂志文章
doi:10.1111/tbed.12304
更新日期:2016-10-01 00:00:00