In Vitro Anticancer Potential of Berberis lycium Royle Extracts against Human Hepatocarcinoma (HepG2) Cells.

Abstract:

:Human liver cancer has emerged as a serious health concern in the world, associated with poorly available therapies. The Berberis genus contains vital medicinal plants with miraculous healing properties and a wide range of bioactivities. In this study, different crude extracts of B. lycium Royle were prepared and screened against Human Hepatocarcinoma (HepG2) cell lines. The water/ethanolic extract of B. lycium Royle (BLE) exhibited significant antiproliferative activity against the HepG2 cancer cell line with an IC50 value of 47 μg/mL. The extract decreased the clonogenic potential of HepG2 cells in a dose-dependent manner. It induced apoptotic cell death in HepG2 cells that were confirmed by cytometric analysis and microscopic examination of cellular morphology through DAPI-stained cells. Biochemical evidence of apoptosis came from elevating the intracellular ROS level that was accompanied by the loss of mitochondrial membrane potential. The mechanism of apoptosis was further confirmed by gene expression analysis using RT-qPCR that revealed the decline in Bcl-2 independent of p53 mRNA and a rise in CDK1 while downregulating CDK5, CDK9, and CDK10 mRNA levels at 48 h of BLE treatment. The most active fraction was subjected to HPLC which indicated the presence of berberine (48 μg/mL) and benzoic acid (15.8 μg/mL) as major compounds in BLE and a trace amount of luteolin, rutin, and gallic acid. Our study highlighted the importance of the most active BLE extract as an excellent source of nutraceuticals against Human Hepatocarcinoma that can serve as an herbal natural cure against liver cancer.

journal_name

Biomed Res Int

authors

Mustafa K,Mohamed H,Shah AM,Yu S,Akhlaq M,Xiao H,Li S,Naz T,Nosheen S,Bai X,Song Y

doi

10.1155/2020/8256809

subject

Has Abstract

pub_date

2020-10-14 00:00:00

pages

8256809

eissn

2314-6133

issn

2314-6141

journal_volume

2020

pub_type

杂志文章
  • Pathophysiology of spasticity: implications for neurorehabilitation.

    abstract::Spasticity is the velocity-dependent increase in muscle tone due to the exaggeration of stretch reflex. It is only one of the several components of the upper motor neuron syndrome (UMNS). The central lesion causing the UMNS disrupts the balance of supraspinal inhibitory and excitatory inputs directed to the spinal cor...

    journal_title:BioMed research international

    pub_type: 杂志文章,评审

    doi:10.1155/2014/354906

    authors: Trompetto C,Marinelli L,Mori L,Pelosin E,Currà A,Molfetta L,Abbruzzese G

    更新日期:2014-01-01 00:00:00

  • Rank-One and Transformed Sparse Decomposition for Dynamic Cardiac MRI.

    abstract::It is challenging and inspiring for us to achieve high spatiotemporal resolutions in dynamic cardiac magnetic resonance imaging (MRI). In this paper, we introduce two novel models and algorithms to reconstruct dynamic cardiac MRI data from under-sampled k - t space data. In contrast to classical low-rank and sparse mo...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2015/169317

    authors: Xiu X,Kong L

    更新日期:2015-01-01 00:00:00

  • Applying the maternal near miss approach for the evaluation of quality of obstetric care: a worked example from a Multicenter Surveillance Study.

    abstract:OBJECTIVE:To assess quality of care of women with severe maternal morbidity and to identify associated factors. METHOD:This is a national multicenter cross-sectional study performing surveillance for severe maternal morbidity, using the World Health Organization criteria. The expected number of maternal deaths was cal...

    journal_title:BioMed research international

    pub_type: 杂志文章,多中心研究

    doi:10.1155/2014/989815

    authors: Haddad SM,Cecatti JG,Souza JP,Sousa MH,Parpinelli MA,Costa ML,Pacagnella RC,Brum IR,Moraes Filho OB,Feitosa FE,Menezes CA,Guanabara EM,Moreira JL,Peret FA,Schmaltz LE,Katz L,Lima AC,Amorim MM,Martins MG,Nascimento D

    更新日期:2014-01-01 00:00:00

  • Image-Based Structural Modeling of the Cardiac Purkinje Network.

    abstract::The Purkinje network is a specialized conduction system within the heart that ensures the proper activation of the ventricles to produce effective contraction. Its role during ventricular arrhythmias is less clear, but some experimental studies have suggested that the Purkinje network may significantly affect the gene...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2015/621034

    authors: Liu BR,Cherry EM

    更新日期:2015-01-01 00:00:00

  • Comparative analysis of context-dependent mutagenesis using human and mouse models.

    abstract::Substitution rates strongly depend on their nucleotide context. One of the most studied examples is the excess of C > T mutations in the CG context in various groups of organisms, including vertebrates. Studies on the molecular mechanisms underlying this mutation regularity have provided insights into evolution, mutag...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2013/989410

    authors: Medvedeva SA,Panchin AY,Alexeevski AV,Spirin SA,Panchin YV

    更新日期:2013-01-01 00:00:00

  • Microelectrical Impedance Spectroscopy for the Differentiation between Normal and Cancerous Human Urothelial Cell Lines: Real-Time Electrical Impedance Measurement at an Optimal Frequency.

    abstract:PURPOSE:To distinguish between normal (SV-HUC-1) and cancerous (TCCSUP) human urothelial cell lines using microelectrical impedance spectroscopy (μEIS). MATERIALS AND METHODS:Two types of μEIS devices were designed and used in combination to measure the impedance of SV-HUC-1 and TCCSUP cells flowing through the channe...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2016/8748023

    authors: Park Y,Kim HW,Yun J,Seo S,Park CJ,Lee JZ,Lee JH

    更新日期:2016-01-01 00:00:00

  • Effect of Direct and Indirect Materials on Stress Distribution in Class II MOD Restorations: A 3D-Finite Element Analysis Study.

    abstract::The aim of this study is to investigate the stress distributions of different restoration options for class II mesio-occluso-distal (MOD) cavities. A class II MOD cavity with proximal box gingival floor 1 mm below cementoenamel junction was designed in a mandibular first molar tooth model. 3D finite-element analysis (...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2020/7435054

    authors: Alp Ş,Gulec Alagoz L,Ulusoy N

    更新日期:2020-12-15 00:00:00

  • The effect of nicotine dependence on psychopathology in patients with schizophrenia.

    abstract:INTRODUCTION:Our study aims to determine the prevalence of nicotine dependence and investigate the effect of nicotine dependence on psychopathology among schizophrenia patients. METHODS:A cross-sectional study was carried out in an outpatient psychiatric clinic at a general hospital in Malaysia. 180 recruited subjects...

    journal_title:BioMed research international

    pub_type: 临床试验,杂志文章

    doi:10.1155/2015/730291

    authors: Yee A,Bt Nek Mohamed NN,Binti Hashim AH,Loh HS,Harbajan Singh MK,Ng CG,Jambunathan ST

    更新日期:2015-01-01 00:00:00

  • Early aging in Chernobyl clean-up workers: long-term study.

    abstract::This paper represents data of long-term open prospective study. 312 male clean-up workers, who participated in elimination of the Chernobyl disaster consequences in 1986-87, were observed and examined in Moscow Research Institute of Psychiatry. The average age of patients was 57,0 ± 6,8 years. All patients were diagno...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2015/948473

    authors: Krasnov V,Kryukov V,Samedova E,Emelianova I,Ryzhova I

    更新日期:2015-01-01 00:00:00

  • A Genomewide Integrative Analysis of GWAS and eQTLs Data Identifies Multiple Genes and Gene Sets Associated with Obesity.

    abstract::To identify novel susceptibility genes and gene sets for obesity, we conducted a genomewide expression association analysis of obesity via integrating genomewide association study (GWAS) and expression quantitative trait loci (eQTLs) data. GWAS summary data of body mass index (BMI) and waist-to-hip ratio (WHR) was dri...

    journal_title:BioMed research international

    pub_type: 杂志文章,meta分析

    doi:10.1155/2018/3848560

    authors: Liu L,Fan Q,Zhang F,Guo X,Liang X,Du Y,Li P,Wen Y,Hao J,Wang W,He A

    更新日期:2018-05-08 00:00:00

  • CD57 expression in incidental, clinically manifest, and metastatic carcinoma of the prostate.

    abstract:OBJECTIVES:CD57 is normally found on NK-cells, but little is known about its expression in prostatic tissue. METHODS:We investigated CD57 expression by immunohistochemistry using tissue microarrays containing 3262 prostate cancers (PCa), lymph node metastases, and benign prostatic tissue. The results were compared wit...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2014/356427

    authors: Wangerin H,Kristiansen G,Schlomm T,Stephan C,Gunia S,Zimpfer A,Weichert W,Sauter G,Erbersdobler A

    更新日期:2014-01-01 00:00:00

  • Blockade of RBP-J-Mediated Notch Signaling Pathway Exacerbates Cardiac Remodeling after Infarction by Increasing Apoptosis in Mice.

    abstract:Background:Ischemic heart disease (IHD) is the major cause of death in patients with cardiovascular disease. Cardiac remodeling is a common pathological change following myocardial infarction (MI), and cardiomyocyte apoptosis plays a key role in this change. Transcription factor recombination signal-binding protein-J (...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2018/5207031

    authors: He Y,Pang S,Huang J,Zhu K,Tong J,Tang Y,Ma G,Chen L

    更新日期:2018-07-03 00:00:00

  • Brain-Derived Neurotropic Factor Val66Met Polymorphism and Posttraumatic Stress Disorder among Survivors of the 1998 Dongting Lake Flood in China.

    abstract:OBJECTIVE:This study mainly aimed to explore the association between brain-derived neurotropic factor (BDNF) Val66Met polymorphism and posttraumatic stress disorder (PTSD) among flood survivors in China. METHODS:Individuals who experienced the 1998 Dongting Lake flood in Southeast Huarong, China, were enrolled in this...

    journal_title:BioMed research international

    pub_type: 临床试验,杂志文章

    doi:10.1155/2017/4569698

    authors: Dai W,Kaminga AC,Wu X,Wen SW,Tan H,Yan J,Deng J,Lai Z,Liu A

    更新日期:2017-01-01 00:00:00

  • Pulmonary Rehabilitation Can Improve the Functional Capacity and Quality of Life for Pneumoconiosis Patients: A Systematic Review and Meta-Analysis.

    abstract::This study evaluated the efficacy and safety of pulmonary rehabilitation (PR) for pneumoconiosis. We systematically searched PubMed, Embase, The Cochrane Library, Web of Science, SinoMed, CNKI, VIP databases and Wanfang Data from their inception to June 1, 2019. A systematic review and meta-analysis of randomized cont...

    journal_title:BioMed research international

    pub_type: 杂志文章,评审

    doi:10.1155/2020/6174936

    authors: Zhao H,Xie Y,Wang J,Li X,Li J

    更新日期:2020-07-28 00:00:00

  • Contrast-induced acute kidney injury: definition, epidemiology, and outcome.

    abstract::Contrast-induced acute kidney injury (CI-AKI) is commonly defined as a decline in kidney function occurring in a narrow time window after administration of iodinated contrast material. The incidence of AKI after contrast material administration greatly depends on the specific definition and cutoff values used. Althoug...

    journal_title:BioMed research international

    pub_type: 杂志文章,评审

    doi:10.1155/2014/859328

    authors: Meinel FG,De Cecco CN,Schoepf UJ,Katzberg R

    更新日期:2014-01-01 00:00:00

  • Risk Factors for Hepatocellular Carcinoma Recurrence and Survival after Liver Transplantation in Patients with HCV-Related Cirrhosis.

    abstract:Purpose:We aimed to identify prognostic factors for survival and recurrence of hepatocellular carcinoma (HCC) after liver transplantation (LT) for patients with HCC and hepatitis C virus-related cirrhosis (HCV-cirrhosis). Methods:This retrospective cohort study followed all adult patients with HCV-cirrhosis who underw...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2020/1487593

    authors: Vidal RIO,Vidal EIO,Pereira BB,Assane CC,Ribeiro A,do Nascimento EM,Romeiro FG,Ribeiro Filho J

    更新日期:2020-10-17 00:00:00

  • Parthenium hysterophorus: a probable source of anticancer, antioxidant and anti-HIV agents.

    abstract::The present work reports the anticancer, antioxidant, lipo-protective, and anti-HIV activities of phytoconstituents present in P. hysterophorus leaf. Dried leaf samples were sequentially extracted with nonpolar and polar solvents. Ethanol fraction showed noticeable cytotoxic activity (81-85%) in SRB assay against MCF-...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2013/810734

    authors: Kumar S,Chashoo G,Saxena AK,Pandey AK

    更新日期:2013-01-01 00:00:00

  • Comparison of Clinical Outcomes of Two-Level PELD and Foraminoplasty PELD for Highly Migrated Disc Herniations: A Comparative Study.

    abstract:Objective:The aim of this study is to compare the clinical outcomes of two-level percutaneous endoscopic lumbar discectomy (PELD) and foraminoplasty PELD in treating highly migrated lumbar disc herniations. Methods:Patients with highly migrated lumbar disc herniations were enrolled from May 2014 to June 2016. Low back...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2019/9681424

    authors: Wu X,Fan G,He S,Gu X,Yang Y

    更新日期:2019-10-13 00:00:00

  • NFIL3 Acts as a Nuclear Factor to Increase Osteosarcoma Progression.

    abstract:Purpose:Osteosarcoma is one of the most common primary malignant, aggressive bone neoplasms. However, the mechanisms of osteosarcoma proliferation, migration, and invasion are not well understood. To explore the possible mechanism of osteosarcoma progression, we used a public database for gene analysis to identify the ...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2019/4068521

    authors: Xu J,Xu G,Zhang T,Chen T,Zhao W,Wang G

    更新日期:2019-11-30 00:00:00

  • New developments in peritoneal fibroblast biology: implications for inflammation and fibrosis in peritoneal dialysis.

    abstract::Uraemia and long-term peritoneal dialysis (PD) can lead to fibrotic thickening of the peritoneal membrane, which may limit its dialytic function. Peritoneal fibrosis is associated with the appearance of myofibroblasts and expansion of extracellular matrix. The extent of contribution of resident peritoneal fibroblasts ...

    journal_title:BioMed research international

    pub_type: 杂志文章,评审

    doi:10.1155/2015/134708

    authors: Witowski J,Kawka E,Rudolf A,Jörres A

    更新日期:2015-01-01 00:00:00

  • The Quantified Level of Circulating Prostate Stem Cell Antigen mRNA relative to GAPDH Level Is a Clinically Significant Indictor for Predicting Biochemical Recurrence in Prostate Cancer Patients after Radical Prostatectomy.

    abstract::The study quantified the relative absolute PSCA level in relation to the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) level in the peripheral blood of 478 hormone-naive prostate cancer (PC) patients who underwent radical prostatectomy from 2005 to 2012 and evaluated its prognostic significance as a risk factor for...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2015/292454

    authors: Kim SH,Park WS,Lee SJ,Choi MK,Yeon SM,Joo JN,Ko A,Lee ES,Joung JY,Seo HK,Chung J,Lee KH

    更新日期:2015-01-01 00:00:00

  • The International Prognostic Index for Patients with Chronic Lymphocytic Leukemia Has the Higher Value in Predicting Overall Outcome Compared with the Barcelona-Brno Biomarkers Only Prognostic Model and the MD Anderson Cancer Center Prognostic Index.

    abstract::In recent years, new prognostic indexes (PIs) for chronic lymphocytic leukemia (CLL), which include clinical, biological, and genetic variables, have been validated, highlighting the MD Anderson Cancer Center prognostic index (MDACC PI), the CLL-international prognostic index (CLL-IPI), and the Barcelona-Brno biomarke...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2018/9506979

    authors: Muñoz-Novas C,Poza-Santaella M,González-Gascón Y Marín I,Hernández-Sánchez M,Rodríguez-Vicente AE,Infante MS,Heras C,Foncillas MÁ,Marín K,Hernández-Rivas JM,Hernández JÁ

    更新日期:2018-03-15 00:00:00

  • Genetic Polymorphisms of IL-17F and TRAF3IP2 Could Be Predictive Factors of the Long-Term Effect of Infliximab against Crohn's Disease.

    abstract:BACKGROUND:We aimed to identify certain genes related to response to infliximab (IFX) and biomarkers to predict the IFX effect for Japanese Crohn's disease (CD) patients by performing an association study of single nucleotide polymorphisms (SNPs) in candidate genes in the interleukin- (IL-) 17 signaling pathway with re...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2015/416838

    authors: Urabe S,Isomoto H,Ishida T,Maeda K,Inamine T,Kondo S,Higuchi N,Sato K,Uehara R,Yajima H,Machida H,Chen CC,Fukuda Y,Takeshima F,Nakao K,Tsukamoto K

    更新日期:2015-01-01 00:00:00

  • The Influence of Humidity on Assessing Irritation Threshold of Ammonia.

    abstract::A large number of occupational exposure limit values (OELs) are based on avoiding of sensory irritation of the eyes and the upper respiratory tract. In order to investigate the chemosensory effect range of a chemical, odor and sensory irritation thresholds (lateralization thresholds, LTs) can be assessed. Humidity aff...

    journal_title:BioMed research international

    pub_type: 杂志文章,随机对照试验

    doi:10.1155/2016/6015761

    authors: Monsé C,Sucker K,Hoffmeyer F,Jettkant B,Berresheim H,Bünger J,Brüning T

    更新日期:2016-01-01 00:00:00

  • An Improved Binary Differential Evolution Algorithm to Infer Tumor Phylogenetic Trees.

    abstract::Tumourigenesis is a mutation accumulation process, which is likely to start with a mutated founder cell. The evolutionary nature of tumor development makes phylogenetic models suitable for inferring tumor evolution through genetic variation data. Copy number variation (CNV) is the major genetic marker of the genome wi...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2017/5482750

    authors: Liang Y,Liao B,Zhu W

    更新日期:2017-01-01 00:00:00

  • Association of p16 as Prognostic Factors for Oropharyngeal Cancer: Evaluation of p16 in 1470 Patients for a 16 Year Study in Northeast China.

    abstract::Human papillomavirus (HPV) is an etiological risk factor for oropharyngeal squamous cell carcinomas (OPSCC). Our study investigates the prevalence, prognostic, and clinicopathologic features of HPV-related oropharyngeal cancer in Northeast China and elucidates the involvement of p16 in the tumorigenesis and progressio...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2018/9594568

    authors: Meng HX,Miao SS,Chen K,Li HN,Yao G,Geng J,Wang H,Shi QT,He J,Mao X,Tong FJ,Wei LL,Sun J,Tan D,You Q,Li X,Geng JS

    更新日期:2018-09-17 00:00:00

  • Clinical and Prognostic Significance of Positive Hepatojugular Reflux on Discharge in Acute Heart Failure: Insights from the ESCAPE Trial.

    abstract::Background. There has been a decline in emphasis of the value of physical examination in heart failure (HF) with increased reliance on cardiac imaging. We aim to study the clinical and prognostic significance of positive hepatojugular reflux (HJR) on discharge in patients hospitalized with HF. Methods. Using the ESCAP...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2017/5734749

    authors: Omar HR,Guglin M

    更新日期:2017-01-01 00:00:00

  • Immunomodulation by Zearalenone in Carp (Cyprinus carpio L.).

    abstract::The mycotoxin zearalenone (ZEN) is a frequent contaminant of animal feeds, but its effects on fish have not yet been investigated extensively. In order to fill this gap a feeding trial with juvenile carp was conducted. Three groups of fish were fed feeds contaminated with ZEN at three concentrations (low: 332 μg kg(-1...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2015/420702

    authors: Pietsch C,Junge R,Burkhardt-Holm P

    更新日期:2015-01-01 00:00:00

  • The Hearing Outcomes of Cochlear Implantation in Waardenburg Syndrome.

    abstract::Objectives. This study aimed to determine the feasibility of cochlear implantation for sensorineural hearing loss in patients with Waardenburg syndrome. Method. A retrospective chart review was performed on patients who underwent cochlear implantation at the University of Tokyo Hospital. Clinical classification, genet...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2016/2854736

    authors: Koyama H,Kashio A,Sakata A,Tsutsumiuchi K,Matsumoto Y,Karino S,Kakigi A,Iwasaki S,Yamasoba T

    更新日期:2016-01-01 00:00:00

  • Cilostazol Improves Proangiogenesis Functions in Human Early Endothelial Progenitor Cells through the Stromal Cell-Derived Factor System and Hybrid Therapy Provides a Synergistic Effect In Vivo.

    abstract::This study investigated the effect of cilostazol on proangiogenesis functions in human early endothelial progenitor cells (EPCs) in vitro and the therapeutic implication of hybrid therapy with cilostazol and human early EPCs in vivo. Cilostazol significantly increased colony-forming units and enhanced differentiation ...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2016/3639868

    authors: Tseng SY,Chao TH,Li YH,Cho CL

    更新日期:2016-01-01 00:00:00