Abstract：
:Osteosarcoma (OS) is a common malignant bone tumor that occurs in adolescents or children under the age of 20, which is extremely difficult to cure and has a high recurrence rate. Recent studies showed that cell division cycle 20 (CDC20) overexpression is associated with poor prognosis in patients with osteosarcoma. However, the function of CDC20 in osteosarcoma has not been investigated clearly. In this study, we aim to explore the role of CDC20 in two independent human OS cell lines' biological phenotype and chemotherapy sensitivity. We applied multiple approaches to measure cell growth, cell cycle, and apoptosis with or without deregulation or overexpression of CDC20. We found that the downregulation of CDC20 by siRNA or apcin suppressed cell proliferation, induced apoptosis, and triggered cell cycle arrest. Consistently, overexpression of CDC20 in normal cells promoted cell growth, inhibited apoptosis. What's more, the additional treatment of siCDC20 or apcin achieved better anticancer effects than that of cisplatin alone. Furthermore, Bim and p21 were upregulated in OS cells following apcin treatment. Altogether, the results of the present study demonstrated that targeting CDC20 could be useful for the treatment of OS, and might be a promising solution for the treatment of the OS with cisplatin insensitivity.
journal_name
Neoplasmajournal_title
Neoplasmaauthors
Gao Y,Guo C,Fu S,Cheng Y,Song Cdoi
10.4149/neo_2020_200614N629subject
Has Abstractpub_date
2020-10-30 00:00:00eissn
0028-2685issn
1338-4317pii
200614N629pub_type
杂志文章相关文献
NEOPLASMA文献大全abstract：:The polarographic reduction of six synthetic 1,3,6-triazine (6-aza) nucleosides with 6-azauracil as the nucleoside base in the strictly anhydrous solutions was studied in the absence and presence of alpha-lipoic acid. The values of the half-wave potentials E1/2 and the parameter of potential carcinogenicity tg alpha w...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:We processed data of 79 patients (pts) with malignant lymphoma from the National Registry of haematopoietic stem cell transplants conducted between 1997 and 2006. The haematopoietic stem cell donor in 48 pts was an HLA matched relative, and in 30 pts an unrelated volunteer. Sixty (77%) pts were transplanted with reduc...
journal_title：Neoplasma
pub_type： 杂志文章,多中心研究
doi：10.4149/neo_2009_01_76
更新日期：2009-01-01 00:00:00
abstract：:This study was supposed to investigate the correlation between the functional single nucleotide polymorphisms (SNPs) (rs2516839 and rs3737787) in USF1 gene and the efficacy and safety of paclitaxel-based chemotherapy and prognosis in the treatment of ovarian cancer (OC). In total 100 OC patients were selected and divi...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2018_170322N205
更新日期：2018-01-01 00:00:00
abstract：:Analysis of new primary tumors following nonmelanoma skin cancers (NMSC) has a public health and risk assessment interest, as well as potential implications for etiologic inference. The aim of this study is to evaluate the risk of the second primary tumors occurrence after NMSC development. A cohort of 2620 cases (133...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Flow cytometry (FCM) was introduced for estimation of DNA content in 75 brain tumors. Among these astrocytomas (38 cases) and meningiomas (16 cases) predominated. Astrocytomas were histologically subdivided into 3 groups of malignancy grade. Aneuploid cell populations were found in 1 out of 4 cases of astrocytoma grad...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:Leucine-rich repeat-containing G protein-coupled receptor 5(LGR5) was identified as the stem cell marker of colon cancer stem cells(CSCs),which were considered as the main criminal cells initiation and reinitiation of colon cancer. We intended to demonstrate and further explain the relationship between LGR5 and colon ...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2014_016
更新日期：2014-01-01 00:00:00
abstract：:Institutional training of prescreening cytotechnicians started in 1972 in the Research Institute of Oncopathology, Budapest. Up to 1983 ten classes, i.e. 230 students have been trained. Authors acknowledge the good achievements of their students and state that the diagnostic reliability of the qualified cytotechnician...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:In a series of 25 patients with cervical carcinoma and 35 patients with vulvar carcinoma, clinically classified (FIGO) as Stage I and Ib, respectively, DNA ploidy and S-phase cell fraction were estimated in paraffin-embedded samples of the primary tumors and their metastases by means of flow cytometry (FCM). The two g...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:The antineoplastic effect of 5-(2-dimethyl-aminoethoxy)-7-oxo-7H-benzo(c) fluorene, substance VUFB 13 468, was studied in leukemia L1210 and leukemia La. It was found that the test substance, in the form of base and in the form of hydrochloride, influenced favorably the survival of leukemic mice, in which the life spa...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：:The determination of patient's resistance to a particular drug contributes to more efficient therapeutical approach. The aim of this study was to evaluate if the responsiveness of Chronic Myeloid Leukemia (CML) patients to Imatinib therapy was predictable from WT1 gene expression in peripheral blood leukocytes. To exa...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2009_05_393
更新日期：2009-01-01 00:00:00
abstract：:All cases of Hodgkin's disease (HD) and non-Hodgkin's lymphoma (NHL) reported to the Kuwait Cancer Registry between 1980-1989 were analyzed. Age specific and age standardized incidence rates were calculated for Kuwaiti and non-Kuwaiti males and females for the two periods of 1980-1984 and 1985-1989, and compared to de...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:Some selected data on cancer statistics in the USSR and results of the nationwide cancer screening activities are presented. The usefullness of some new methods in prophylactic examination is discussed. ...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1975-01-01 00:00:00
abstract：:In this study subcellular localization of cyclin A in the human promyelocytic leukemia cell line HL-60 and the human erythroleukemic K-562 cell line was examined by immunocytochemical methods. Studies were based on light and electron microscope evaluations. Cyclin A at the level of light microscope was present in 48% ...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Leukemia-associated phenotypes have been suggested to be a valuable tool for the detection of minimal residual disease in acute leukemia patients, as they allow to distinguish leukemic blasts from normal hematopoietic progenitor cells. The aim of the present study was to analyze the proportion of acute leukemia patien...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:On the basis of the author's own results and literary data emphasis is laid on the significance of lymphography for confirming or establishing a diagnosis, for determining the extent of a lesion and for proper considerations on indications for surgical treatment. Relymphography following a surgical intervention is cap...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1975-01-01 00:00:00
abstract：:Extranodal NK/T-cell lymphoma nasal type (ENKTL) is a subtype of T cell lymphoma with poor prognosis. In this study, we designed a new prognostic model specifically for ENKTL to improve the risk stratification. In 29 ENKTL patients, we screened mutations in 9 ENKTL-associated genes using next-generation sequencing (NG...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2020_190307N194
更新日期：2020-05-01 00:00:00
abstract：:We report on 12 patients who fulfilled the FAB diagnosis criteria of chronic myelomonocytic leukemia (CMML). Some clinical findings (splenomegaly) as well as biological parameters (WBC count, cellularity of bone marrow sections, presence of dyserythropoiesis in bone marrow smears) were subjected to particular analysis...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:The influence of two kinds of transplantable melanomas on the secretory function of peritoneal macrophages, and particularly the release of TNF-alpha has been studied. The results showed a statistically significant increase of protein content in the supernatants from 24 h cultured macrophages from melanoma-bearing ham...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:The concentration of estradiol and progesterone receptors in tumor cytosol from human breast cancer patients represents an important factor for the selection of patients for endocrine therapy and for the prognosis of the disease. This report presents results obtained by measurement of estradiol receptor in 157 breast ...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:Dendritic cells are able to induce anti-tumor immune responses by presenting tumor-specific antigens to T-lymphocytes. Various tumor-associated antigens have been studied in multiple myeloma in an effort to find a strong antigen capable of generating clinically meaningful responses in vaccinated patients. The aim of o...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2010_05_455
更新日期：2010-01-01 00:00:00
abstract：:Sixty-five patients with Stage I and II endometrial carcinoma were investigated. After a short-time follow-up (17-24 months) significant differences in frequency of relapses were observed between patients with tumors containing low amounts of estrogen receptor (ER less than 60 fmol/mg DNA) in the nuclear pellet, and t...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:The level of lipid-bound sialic acid (LSA) was followed in inbred C57Bl/6 mice bearing tumors induced by benzpyrene at a dose of 20 mg per kg of body weight. In the plasma of mice with macroscopically ascertained tumors, the level of LSA was increased significantly. The LSA level was also significantly increased in th...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:The possible association between cigarette smoking and the risk of benign breast disease (BBD) was assessed in a case-control study conducted in Gdańsk, Poland, between 1990 and 1994. The study compared 160 women with newly diagnosed BBD admitted to the Gdańsk Cancer Outpatients Clinic and 160 controls, women from out...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Lectin dependent cell mediated cytotoxicity (LDCC) to autologous tumor cells (ATC) by the tumor associated macrophages (TAM) was studied by 4 h 51Cr release assays at early and late stages of growth of a murine transplantable ascites tumor, S-180, in presence of different concentrations of wheat germ agglutinin (WGA) ...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:Already a trace of oxygen mediates the one-electron transfer from 3,4-benzopyrene (benzo(a)pyrene, BP) to hydrogen peroxide or to tert. butyl hydroperoxide (ROOH), leading in this way to generation of highly reactive HO X, HO2 X or RO X and RO2 X radicals in nonpolar solvents at biological temperatures. At slightly hi...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
abstract：:In 41 patients with Hodgkin's disease nitroblue tetrazolium (NBT) dye reduction with resting and stimulated granulocytes, before, in the course and after chemotherapy was examined. Twofold decrease of the capability of NBT reduction test with stimulated granulocytes was found, whereas with resting granulocytes only sl...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:Considering the potency of circRNAs in manifesting neoplastic progression, we attempted to explore the feasibility of applying ciRS-7 for diagnosis and prognosis estimation of cervical cancer (CC). Here 352 CC patients, 204 cervical intraepithelial neoplasia (CIN) patients and 227 healthy controls were recruited. The ...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2019_190415N334
更新日期：2020-03-01 00:00:00
abstract：:The objective of the present study was to evaluate clinical condition and results of surgical treatment of patients with typical and atypical bronchial carcinoids. The study was based on retrospective analysis of a total of 96 patients (mean age 47.3 year, age range 21-76, 44 men and women 52), who were surgically tre...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:Helicase POLQ-like (HELQ), an ATP-dependent 3'-5' DNA helicase, plays pivotal roles in DNA processing, such as DNA replication, recombination and repair. The recent discovery of HELQ in mouse model and human cancer cell reveal that genetic alteration of HELQ is involved in cancer susceptibility and subfertility. Here ...
journal_title：Neoplasma
pub_type： 杂志文章,评审
doi：10.4149/neo_2016_601
更新日期：2016-01-01 00:00:00
abstract：:Currently, mechanisms leading to both apoptosis induction and the development of hormone-independence of prostate carcinoma cells are intensively studied. Attention is also given to the possibility of restoring cell sensitivity to hormone-antagonists. The present study focuses on the effect of the combined synthetic c...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
