Abstract:
:DREADDs are chemogenetic tools widely used to remotely control cellular signaling, neuronal activity, and behavior. Here we used a structure-based approach to develop a new Gi-coupled DREADD using the kappa-opioid receptor as a template (KORD) that is activated by the pharmacologically inert ligand salvinorin B (SALB). Activation of virally expressed KORD in several neuronal contexts robustly attenuated neuronal activity and modified behaviors. Additionally, co-expression of the KORD and the Gq-coupled M3-DREADD within the same neuronal population facilitated the sequential and bidirectional remote control of behavior. The availability of DREADDs activated by different ligands provides enhanced opportunities for investigating diverse physiological systems using multiplexed chemogenetic actuators.
journal_name
Neuronjournal_title
Neuronauthors
Vardy E,Robinson JE,Li C,Olsen RHJ,DiBerto JF,Giguere PM,Sassano FM,Huang XP,Zhu H,Urban DJ,White KL,Rittiner JE,Crowley NA,Pleil KE,Mazzone CM,Mosier PD,Song J,Kash TL,Malanga CJ,Krashes MJ,Roth BLdoi
10.1016/j.neuron.2015.03.065subject
Has Abstractpub_date
2015-05-20 00:00:00pages
936-946issue
4eissn
0896-6273issn
1097-4199pii
S0896-6273(15)00289-5journal_volume
86pub_type
杂志文章相关文献
NEURON文献大全abstract::It has been hypothesized that the direction of synaptic weight change elicited by synaptic activity depends on the magnitude of the activity-dependent rise in intracellular Ca2+ concentration. Several aspects of this hypothesis were examined at the Schaffer collateral CA1 synapse, where both long-term depression (LTD)...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(00)80102-6
更新日期:1996-04-01 00:00:00
abstract::Incoming stimuli are encoded collectively by populations of cortical neurons, which transmit information by using a neural code thought to be predominantly redundant. Redundant coding is widely believed to reflect a design choice whereby neurons with overlapping receptive fields sample environmental stimuli to convey ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2019.07.006
更新日期:2019-10-23 00:00:00
abstract::In this issue of Neuron, Costa et al. (2017) introduce a theoretical framework that predicts the ratio of presynaptic and postsynaptic changes taking place during LTP and LTD and show that these processes co-operate so as to optimize the postsynaptic response statistics. ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2017.09.022
更新日期:2017-09-27 00:00:00
abstract::The level of electrotonic coupling in the inferior olive is extremely high, but its functional role in cerebellar motor control remains elusive. Here, we subjected mice that lack olivary coupling to paradigms that require learning-dependent timing. Cx36-deficient mice showed impaired timing of both locomotion and eye-...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2008.03.016
更新日期:2008-05-22 00:00:00
abstract::Somatic mutation of the MTOR gene is a genetic etiology of focal malformations of cortical development. In this issue of Neuron, Park et al. (2018) identify defective autophagy-dependent ciliogenesis/Wnt signaling as an underlying mechanism affecting neuronal migration and cortical lamination. ...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/j.neuron.2018.06.042
更新日期:2018-07-11 00:00:00
abstract::The prefrontal cortex (PFC) plays a key role in controlling goal-directed behavior. Although a variety of task-related signals have been observed in the PFC, whether they are differentially encoded by various cell types remains unclear. Here we performed cellular-resolution microendoscopic Ca(2+) imaging from genetica...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2015.06.021
更新日期:2015-07-15 00:00:00
abstract::Application of Clostridium difficile toxin B, an inhibitor of the Rho family of GTPases, at the Aplysia sensory to motor neuron synapse blocks long-term facilitation and the associated growth of new sensory neuron varicosities induced by repeated pulses of serotonin (5-HT). We have isolated cDNAs encoding Aplysia Rho,...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2005.01.044
更新日期:2005-03-24 00:00:00
abstract::Successful visual navigation requires a sense of the geometry of the local environment. How do our brains extract this information from retinal images? Here we visually presented scenes with all possible combinations of five scene-bounding elements (left, right, and back walls; ceiling; floor) to human subjects during...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2019.04.014
更新日期:2019-07-03 00:00:00
abstract::Experience-dependent plasticity in the adult brain has clinical potential for functional rehabilitation following central and peripheral nerve injuries. Here, plasticity induced by unilateral infraorbital (IO) nerve resection in 4-week-old rats was mapped using MRI and synaptic mechanisms were elucidated by slice elec...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2012.04.024
更新日期:2012-05-24 00:00:00
abstract::We have studied the subcellular distribution of the alpha 1 and alpha 2 subunits of the dihydropyridine (DHP) receptor and ankyrin in rat skeletal muscle with immunofluorescence and immunogold labeling techniques. All three proteins were concentrated in the triad junction formed between the T-tubules and sarcoplasmic ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(90)90170-k
更新日期:1990-09-01 00:00:00
abstract::It is a commonly held view that numbers are represented in an abstract way in both parietal lobes. This view is based on failures to find differences between various notational representations. Here we show that by using relatively smaller voxels together with an adaptation paradigm and analyzing subjects on an indivi...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2006.12.025
更新日期:2007-01-18 00:00:00
abstract::Spinal and bulbar muscular atrophy (SBMA) is a polyglutamine disease caused by the expansion of a CAG repeat in the androgen receptor (AR) gene. We generated a transgenic mouse model carrying a full-length AR containing 97 CAGs. Three of the five lines showed progressive muscular atrophy and weakness as well as diffus...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(02)00834-6
更新日期:2002-08-29 00:00:00
abstract::Using a transgenic mouse line in which GFP is expressed in a single population of retinal ganglion cells (RGCs), Huberman and colleagues report in this issue of Neuron that the axon terminals of RGCs exhibit an orderly pattern of distribution in the higher visual centers. This pattern undergoes a developmental refinem...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/j.neuron.2008.08.001
更新日期:2008-08-14 00:00:00
abstract::Cognitive deficits, core features of mental illness, largely result from dysfunction of prefrontal networks. This dysfunction emerges during early development, before a detectable behavioral readout, yet the cellular elements controlling the abnormal maturation are still unknown. Here, we address this open question by...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2019.09.042
更新日期:2020-01-08 00:00:00
abstract::The suprachiasmatic nucleus (SCN), the primary circadian pacemaker in mammals, is a network structure composed of multiple types of neurons. Here, we report that mice with a Bmal1 deletion specific to arginine vasopressin (AVP)-producing neurons showed marked lengthening in the free-running period and activity time of...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2015.02.005
更新日期:2015-03-04 00:00:00
abstract::Miniature synaptic currents have long been known to represent random transmitter release under resting conditions, but much remains to be learned about their nature and function in central synapses. In this work, we describe a new class of miniature currents ("preminis") that arise by the autocrine activation of axona...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2010.03.030
更新日期:2010-04-29 00:00:00
abstract::Perceptual decisions require the transformation of raw sensory inputs into cortical representations suitable for stimulus discrimination. One of the best-known examples of this transformation involves the middle temporal area (MT) of the primate visual cortex. Area MT provides a robust representation of stimulus motio...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2017.06.024
更新日期:2017-07-19 00:00:00
abstract::Voltage-gated calcium channels couple depolarization of the cell-surface membrane to entry of calcium, which triggers secretion, contraction, neurotransmission, gene expression, and other physiological responses. They are encoded by ten genes, which generate three voltage-gated calcium channel subfamilies: CaV1; CaV2;...
journal_title:Neuron
pub_type: 杂志文章,评审
doi:10.1016/j.neuron.2018.03.017
更新日期:2018-05-02 00:00:00
abstract::A male zebra finch learns a song by listening to a tutor, but song learning is normally restricted to a critical period in juvenile development. Here we identify an RNA whose expression in the song control circuit is altered during this critical period. The RNA encodes a soluble presynaptic protein that forms a predic...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(95)90040-3
更新日期:1995-08-01 00:00:00
abstract::Conditioned appetitive and aversive responses (CRs) are thought to result from the activation of specific subsets of valence-coding basolateral amygdala (BLA) neurons. Under this model, the responses of BLA cells to conditioned stimuli (CSs) and the activity that drives CRs are closely related. We tested the strength ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2018.07.036
更新日期:2018-09-19 00:00:00
abstract::Synaptogenesis, the generation and maturation of functional synapses between nerve cells, is an essential step in the development of neuronal networks in the brain. It is thought to be triggered by members of the neuroligin family of postsynaptic cell adhesion proteins, which may form transsynaptic contacts with presy...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2006.09.003
更新日期:2006-09-21 00:00:00
abstract::Two mutations in the sodium channel alpha subunit that have been implicated as the cause of periodic paralysis were studied by functional expression in a mammalian cell line. Both mutations disrupted inactivation without affecting the time course of the onset of the sodium current or the single-channel conductance. Th...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(93)90321-h
更新日期:1993-02-01 00:00:00
abstract::Our tactile perception of external objects depends on skin-object interactions. The mechanics of contact dictates the existence of fundamental spatiotemporal input features-contact initiation and cessation, slip, and rolling contact-that originate from the fact that solid objects do not interpenetrate. However, it is ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2014.07.038
更新日期:2014-09-17 00:00:00
abstract::We recently reported that homozygous Presenilin-1 (Psen1) knockin (KI) mice carrying the familial Alzheimer's disease (FAD) mutation L435F or C410Y recapitulate the phenotypes of Psen1(-/-) mice. Production and steady-state levels of Aβ40 and Aβ42 are undetectable in KI/KI brains and reduced in KI/+ brains, though the...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/j.neuron.2016.03.009
更新日期:2016-04-20 00:00:00
abstract::Firing patterns of neurons in the orbitofrontal cortex (OF) were analyzed in rats trained to perform a task that encouraged incidental associations between distinct odors and the places where their occurrence was detected. Many of the neurons fired differentially when the animals were at a particular location or sampl...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(00)81095-8
更新日期:1999-02-01 00:00:00
abstract::The role of early visual experience in the establishment of human high-order visual areas is poorly understood. Here we investigated this issue using human amblyopia--a developmental visual disorder, which manifests a central vision (acuity) deficit. Previous fMRI studies of amblyopes have described abnormal functiona...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(03)00720-7
更新日期:2003-12-04 00:00:00
abstract::To characterize the role of rare complete human knockouts in autism spectrum disorders (ASDs), we identify genes with homozygous or compound heterozygous loss-of-function (LoF) variants (defined as nonsense and essential splice sites) from exome sequencing of 933 cases and 869 controls. We identify a 2-fold increase i...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2012.12.029
更新日期:2013-01-23 00:00:00
abstract::The capsaicin receptor, TRPV1, is regulated by phosphatidylinositol-4,5-bisphosphate (PIP(2)), although the precise nature of this effect (i.e., positive or negative) remains controversial. Here, we reconstitute purified TRPV1 into artificial liposomes, where it is gated robustly by capsaicin, protons, spider toxins, ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2012.12.016
更新日期:2013-02-20 00:00:00
abstract::The study of patients with brain lesions has made major historical contributions to cognitive neuroscience. Here I argue for an increased investment in modern lesion mapping, complementing fMRI studies and laying the conceptual and analytic foundations for future techniques that could experimentally manipulate human b...
journal_title:Neuron
pub_type: 历史文章,杂志文章
doi:10.1016/j.neuron.2016.05.014
更新日期:2016-06-15 00:00:00
abstract::Development of neuronal circuits is controlled by evolutionarily conserved axon guidance molecules, including Slits, the repulsive ligands for roundabout (Robo) receptors, and Netrin-1, which mediates attraction through the DCC receptor. We discovered that the Robo3 receptor fundamentally changed its mechanism of acti...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2014.11.004
更新日期:2014-12-17 00:00:00