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A method for the examination of SDHI fungicide resistance mechanisms in phytopathogenic fungi using a heterologous expression system in Sclerotinia sclerotiorum.

Abstract：

:Succinate dehydrogenase inhibitors (SDHI) comprise a class of broad-spectrum fungicides utilized for management of diseases caused by phytopathogenic fungi. In many cases, reduced sensitivity to SDHI fungicides has been correlated with point mutations in the SdhB and SdhC target genes that encode components of the succinate dehydrogenase complex. The genetic basis of SDHI fungicide resistance mechanisms, however, has been functionally characterized in very few fungi. Sclerotinia sclerotiorum is a fast-growing and SDHI fungicide-sensitive phytopathogenic fungus that can be conveniently transformed. Given the high amino acid sequence similarity and putative structural similarity of SDHI protein target sites between S. sclerotiorum and other common phytopathogenic ascomycete fungi, we developed an in vitro heterologous expression system that used S. sclerotiorum as a reporter strain. With this system, we were able to demonstrate the function of mutant SdhB or SdhC alleles from several ascomycete fungi in conferring resistance to multiple SDHI fungicides. In total, we successfully validated the function of Sdh alleles that had been previously identified in field isolates of Botrytis cinerea, Blumeriella jaapii, and Clarireedia jacksonii (formerly S. homoeocarpa) in conferring resistance to boscalid, fluopyram or fluxapyroxad, and used site-directed mutagenesis to construct and phenotype a mutant allele that is not yet known to exist in Monilinia fructicola populations. We also examined the functions of these alleles in conferring cross-resistance to more recently introduced SDHIs including inpyrfluxam, pydiflumetofen, and pyraziflumid. The approach developed in this study can potentially be widely applied to interrogate SDHI fungicide resistance mechanisms in other phytopathogenic ascomycetes.

journal_name

Phytopathology

journal_title

Phytopathology

authors

Peng J,Sang H,Proffer T,Gleason J,Outwater C,Jung G,Sundin GW

doi

10.1094/PHYTO-09-20-0421-R

subject

Has Abstract

pub_date

2020-11-03 00:00:00

eissn

0031-949X

issn

1943-7684

pub_type

杂志文章

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A method for the examination of SDHI fungicide resistance mechanisms in phytopathogenic fungi using a heterologous expression system in Sclerotinia sclerotiorum.