Immunogenic virus-like particles continuously expressed in mammalian cells as a veterinary rabies vaccine candidate.

Abstract:

:Rabies is one of the most lethal infectious diseases in the world, with a mortality approaching 100%. There are between 60,000 and 70,000 reported annual deaths, but this is probably an underestimation. Despite the fact that there are vaccines available for rabies, there is a real need of developing more efficacious and cheaper vaccines. This is particularly true for veterinary vaccines because dogs are still the main vector for rabies transmission to human beings. In a previous work, we described the development and characterization of rabies virus-like particles (RV-VLPs) expressed in HEK293 cells. We showed that RV-VLPs are able to induce a specific antibodies response. In this work, we show that VLPs are able to protect mice against virus challenge. Furthermore, we developed a VLPs expressing HEK-293 clone (sP2E5) that grows in serum free medium (SFM) reaching high cell densities. sP2E5 was cultured in perfusion mode in a 5 L bioreactor for 20 days, and the RV-VLPs produced were capable of triggering a protective immune response without the need of concentration or adjuvant addition. Further, these VLPs are able to induce the production of rabies virus neutralizing antibodies. These results demonstrate that RV-VLPs are a promising rabies vaccine candidate.

journal_name

Vaccine

journal_title

Vaccine

authors

Fontana D,Kratje R,Etcheverrigaray M,Prieto C

doi

10.1016/j.vaccine.2015.03.088

subject

Has Abstract

pub_date

2015-08-20 00:00:00

pages

4238-46

issue

35

eissn

0264-410X

issn

1873-2518

pii

S0264-410X(15)00418-1

journal_volume

33

pub_type

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