Ammonium removal at low temperature by a newly isolated heterotrophic nitrifying and aerobic denitrifying bacterium Pseudomonas fluorescens wsw-1001.

Abstract:

:A heterotrophic nitrifier wsw-1001 was isolated from Songhua River and identified as Pseudomonas fluorescens. Ammonium removal by the strain at low temperature was investigated. The effect of initial ammonium concentration (from 5 to 1000 mg/L) and culture temperature (from 4°C to 30°C) on ammonium removal efficiency was studied. Biodegradation product, [Formula: see text], [Formula: see text], N2, N2O and intercellular N were monitored. The results indicated that the strain had potential for water and wastewater treatment. Ammonium could be removed by the strain at low temperature. Ammonium removal efficiency increased with temperature from 4°C to 20°C and decreased with ammonium concentration from 5 to 1000 mg/L. The strain exhibited a capability of heterotrophic nitrification and aerobic denitrification using [Formula: see text] as the sole nitrogen source at 8°C. [Formula: see text] and [Formula: see text] were reduced by the strain. Nitrogen balance analysis in the presence of 39.7 mg/L [Formula: see text] indicated that 71.2% [Formula: see text] was removed by converting to N2 (46.3%) and assimilating as biomass (42.5%). Substances such as [Formula: see text], [Formula: see text] and N2O were detected at very low concentrations. Ammonium mono-oxygenase, hydroxylamine oxidase, nitrite reductase and nitrate reductase activity were measured. The ammonium removal pathway of the strain was speculated to be [Formula: see text].

journal_name

Environ Technol

journal_title

Environmental technology

authors

Zhang S,Sha C,Jiang W,Li W,Zhang D,Li J,Meng L,Piao Y

doi

10.1080/09593330.2015.1035759

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

2488-94

issue

19

eissn

0959-3330

issn

1479-487X

journal_volume

36

pub_type

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