Analysis of muscle fiber clustering in the diaphragm muscle of sarcopenic mice.

Abstract:

INTRODUCTION:Sarcopenia likely comprises muscle fiber denervation and re-innervation, resulting in clustering of muscle fibers of the same type (classified by myosin heavy chain isoform composition). Development of methodology to quantitatively evaluate clustering of muscle fibers according to fiber type is necessary. METHODS:Fiber type specific immunofluorescence histology was used to quantify fiber clustering in murine diaphragm muscle (n = 15) at ages 6 and 24 months. RESULTS:With age, fiber type clustering is evidenced by fiber type specific changes in distances between fibers, specifically a 14% decrease to the closest fiber for type I and 24% increase for type IIx and/or IIb fibers (P < 0.001). Additionally, a 34% increase to the 3 closest type IIx and/or IIb fibers was found (P < 0.001). CONCLUSIONS:This novel method of analyzing fiber type clustering may be useful in examining pathophysiological conditions of motor unit loss in neuromuscular disorders, myopathies, dystrophies, injuries, or amyotrophic lateral sclerosis.

journal_name

Muscle Nerve

journal_title

Muscle & nerve

authors

Greising SM,Medina-Martínez JS,Vasdev AK,Sieck GC,Mantilla CB

doi

10.1002/mus.24641

subject

Has Abstract

pub_date

2015-07-01 00:00:00

pages

76-82

issue

1

eissn

0148-639X

issn

1097-4598

journal_volume

52

pub_type

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