Abstract:
:This study aimed to explore effects of static magnetic fields (SMFs) of moderate intensity (3-50 mT) as biophysical stimulators of proliferation and osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells (MSCs). MSCs were exposed to SMFs of three intensities: 3, 15, and 50 mT. Proliferation was assessed by cell counting and bromodeoxyuridine incorporation, and differentiation by measuring alkaline phosphatase (ALP) activity, calcium content, mineralized nodule formation, and transcripts of osteogenic markers. Moderate intensity SMFs increased cell proliferation, ALP activity, calcium release, and mineralized nodule formation in a dose- and time-dependent manner, which peaked at 15 mT. In the same manner, they upregulated expression of osteogenic marker genes such as ALP, bone sialoprotein 2 (BSP2), collagen1a1 (COL1a1), osteocalcin (OCN), osteonectin (ON), osteopontin (OPN), osterix (OSX), and runt-related transcription factor 2 (RUNX2) with peak at 15 mT after 14 or 21 days of exposure. Results demonstrate that moderate intensity SMFs promote proliferation and osteoblastic differentiation of MSCs. This effect could help to improve MSC responses during osseointegration between a dental implant and surrounding bone.
journal_name
Bioelectromagneticsjournal_title
Bioelectromagneticsauthors
Kim EC,Leesungbok R,Lee SW,Lee HW,Park SH,Mah SJ,Ahn SJdoi
10.1002/bem.21903subject
Has Abstractpub_date
2015-04-01 00:00:00pages
267-76issue
4eissn
0197-8462issn
1521-186Xjournal_volume
36pub_type
杂志文章abstract::B lymphocytes collected from normal ICR Swiss mouse spleens were exposed in vitro in a Crawford cell to 147-MHz radiofrequency (RF) radiation, amplitude modulated by a 9-, 16-, or 60-Hz sine wave. The power densities ranged between 0.11 and 48 mW/cm2. The irradiated samples and the controls were maintained at 37 degre...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250040206
更新日期:1983-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.10023
更新日期:2002-05-01 00:00:00
abstract::Mice were exposed in the far field in an anechoic chamber to 2,880-MHz pulsed microwaves 3 to 7.5 h daily, 5 days/week for 60 to 360 h. Three experiments were performed at average power densities of 5 mW/cm2 and six at 10 mW/cm2, corresponding to averaged specific absorption rates (SARs) of 2.25 and 4.50 mW/g, respect...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250040409
更新日期:1983-01-01 00:00:00
abstract::This article presents measurement methods used to determine the human exposure to electromagnetic fields radiated from operating base stations. In Korea, when evaluating the human exposure to electromagnetic fields from operating base stations, the measurement procedure is different between the following cases: in sit...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20416
更新日期:2008-10-01 00:00:00
abstract::The effects of mm-waves 60.22-62.22 GHz and 75 GHz on A-type K+ currents and the effects of 61.22 GHz on Ca2+ currents of Lymnaea neurons were investigated using a whole-cell voltage-clamp technique. The open end of a rectangular waveguide covered with a thin Teflon film served as a radiator. Specific absorption rates...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::The electromagnetic exposure of cell phone users depends on several parameters. One of the most dominant of these is the distance between the cell phone and the head tissue. The pinna can be regarded as a spacer between the top of the phone and the head tissue. The size of this spacer has not yet been systematically s...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20575
更新日期:2010-07-01 00:00:00
abstract::Experiments were conducted to see whether the cellular response to electromagnetic (EM) fields occurs through a detection process involving temporal sensing. L929 cells were exposed to 60 Hz magnetic fields and the enhancement of ornithine decarboxylase (ODC) activity was measured to determine cellular response to the...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/(sici)1521-186x(1997)18:5<388::aid-bem6>3.
更新日期:1997-01-01 00:00:00
abstract::To evaluate duration- and dose-dependent effects of continuous exposure to a 60 Hz magnetic field (MF) on the testes in mice, BALB/c male mice were exposed to a 60 Hz MF at 100 μT for 24 h a day for 2, 4, 6, or 8 weeks, and at 2, 20, or 200 μT for 24 h a day for 8 weeks. Any exposures to MF did not significantly affec...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.21819
更新日期:2014-02-01 00:00:00
abstract::The effects of millimeter microwaves in the frequency range of 54-76 GHz on capacitance and conductance of lipid bilayer membranes (BLM) were studied. Some of the membranes were modified by gramicidin A and amphotericin B or by tetraphenylboron anions (TPhB-). The millimeter microwaves were pulse-modulated (PW) at rep...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250160209
更新日期:1995-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250090208
更新日期:1988-01-01 00:00:00
abstract::In this article, the transmembrane voltage induced on erythrocyte, codocyte, ovalocyte and spherocyte cell models exposed to a linearly polarised electromagnetic plane wave of frequency 1800 MHz is calculated. For this purpose, a finite element (FE) numerical technique with adaptive meshing is used. The results show t...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20065
更新日期:2004-12-01 00:00:00
abstract::Accumulating evidence does not yet confirm the effect of power line frequency magnetic field (MF) on human health and fertility. We recently reported that, at continuous 60 Hz MF exposure in mice, the dose given as magnetic flux density (tesla; T) and duration of exposure was related to induce testicular germ cell apo...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.22146
更新日期:2018-10-01 00:00:00
abstract::The MobilEe-study was the first cross-sectional population-based study to investigate possible health effects of mobile communication networks on children using personal dosimetry. Exposure was assessed every second resulting in 86,400 measurements over 24 h for each participant. Therefore, a functional approach to an...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20477
更新日期:2009-05-01 00:00:00
abstract::Through the use of the non-invasive vibrating probe technique for detecting extracellular ionic currents developed in 1974 [Jaffe and Nuccitelli: J Cell Biol 63:614-628, 1974], embryonic currents have been detected in a wide range of animal systems (recently reviewed in [Nuccitelli, Noninvasive Techniques in Cell Biol...
journal_title:Bioelectromagnetics
pub_type: 杂志文章,评审
doi:10.1002/bem.2250130714
更新日期:1992-01-01 00:00:00
abstract::As a result of ischaemia/reperfusion, massive generation of reactive oxygen species occurs, followed by decreased activity of antioxidant enzymes. Extremely low frequency electromagnetic fields (ELF-EMF) can modulate oxidative stress, but there are no clinical antioxidant studies in brain stroke patients. The aim of o...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.22055
更新日期:2017-07-01 00:00:00
abstract::We have previously demonstrated that exposure of Chinese hamster lung (CHL) cells to 50 Hz magnetic fields (MFs) and/or 12-O-tetradecanoylphorbol-3-acetate (TPA)-inhibited gap junctional intercellular communication (GJIC). To explore and compare the mechanisms of GJIC inhibition induced by extremely low frequency (ELF...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.10070
更新日期:2003-02-01 00:00:00
abstract::Electromagnetic sensibility refers to the ability to perceive the electromagnetic field (EMF) without necessarily developing health symptoms attributed to EMF exposure. A large sample of young healthy adults (n = 84) performed two forced-choice tasks on the perception of the GSM mobile phone EMF (902 MHz pulsed at 217...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20375
更新日期:2008-02-01 00:00:00
abstract::Most of the research concerning magnetic antinociception was focused on brief exposure less than 1 h. The main purpose of the present study was to determine the effect of extremely low frequency (ELF) magnetic field (MF) repeated exposures on rats in inducing antinociception and to find the effective analgesic "time w...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20232
更新日期:2006-09-01 00:00:00
abstract::The objective was to study the risk of cytogenetic damage among high voltage laboratory workers exposed to electromagnetic fields and mineral oil. This is a cross sectional study of 24 exposed and 24 matched controls in a Norwegian transformer factory. The exposure group included employees in the high voltage laborato...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.33
更新日期:2001-04-01 00:00:00
abstract::Drosophila larvae reared inside a micro-metal box with an internal field strength 0.004 microT, were treated with a magnetic field of 50 Hz, 8 microT. for 20 min. Control experienced 0.004 microT. Cellular transcript levels were assessed using slot blots and quantified using a Phosphorimager. Blots were hybridised usi...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::A new method for finding the dielectric parameters of biological substances is presented. The method makes use of the loss-tangent as a function of frequency to identify the dominating relaxation process. The method was tested for a few cell suspensions (blood and lymphocytes) and two tissues (liver and spleen). The o...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250070303
更新日期:1986-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20262
更新日期:2007-01-01 00:00:00
abstract::This study was a meta-analysis to examine whether electrical stimulation has specific effects in the healing of musculoskeletal repair process and in the diminution of symptoms with bone and joint disorders. Using MEDLINE (1966-1999) and EMBASE (1985-1999) a search for articles was carried out with four medical subjec...
journal_title:Bioelectromagnetics
pub_type: 杂志文章,meta分析
doi:10.1002/bem.106
更新日期:2002-02-01 00:00:00
abstract::The goal of the research reported here is to narrow the range of uncertainty about peripheral nerve stimulation (PNS) thresholds associated with whole body magnetic field exposures at 50/60 Hz. This involved combining PNS thresholds measured in human subjects exposed to pulsed magnetic gradient fields with calculation...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20106
更新日期:2005-09-01 00:00:00
abstract::Data are presented on ankle-specific SARs and foot currents as a function of strengths of radio-frequency electromagnetic fields encountered by operators of dielectric heaters. The determination of foot currents was based on near-field exposures in which reactive coupling dominates, and which can result in substantial...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250130204
更新日期:1992-01-01 00:00:00
abstract::Treatment with direct electric current (DC) can inhibit tumor growth in several systems. To evaluate the cellular reactions generated by this treatment, we stimulated mouse mastocytoma P815 cells with DC and examined their viability and ultrastructural characteristics, as well as the effect of DC on surface carbohydra...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/1521-186x(200012)21:8<597::aid-bem6>3.0.co
更新日期:2000-12-01 00:00:00
abstract::Findings from prior studies of possible health and physiological effects from mobile phone use have been inconsistent. Exposure periods in provocation studies have been rather short and personal characteristics of the participants poorly defined. We studied the effect of radiofrequency field (RF) on self-reported symp...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20379
更新日期:2008-04-01 00:00:00
abstract::The effects of hyperthermia, alone and in conjunction with microwave exposure, on brain energetics were studied in anesthetized male Sprague-Dawley rats. The effect of temperature on adenosine triphosphate concentration [ATP] and creatine phosphate concentration [CP] was determined in the brains of rats that were main...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250050107
更新日期:1984-01-01 00:00:00
abstract::The fundamental intramolecular frequency of a globular protein can be obtained from the measurements of acoustic velocities of bulk protein matter. This lowest frequency for common size molecules is shown to be above several hundred GHz. All modes below this frequency would then be intermolecular modes or bulk modes o...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20013
更新日期:2004-09-01 00:00:00
abstract::In this study, we measured and analyzed the spectral characteristics of a low-frequency magnetic field (MF) inside several gasoline-powered cars while driving on busy city roads. The spectra obtained upon measurements in the interior of the cars are compared with those measured in office locations at different times o...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.22269
更新日期:2020-07-01 00:00:00