The Improvement of Immune Effect of Recombinant Human Beta-Defensin 2 on Hepatitis B Vaccine in Mice.


:Immunization with hepatitis B vaccine is an effective measure for prevention and control of hepatitis B Virus (HBV) infection. Although lots of efforts to improve the effect of hepatitis B vaccine have been made, the function of human beta defensin 2 (hBD2) on hepatitis B vaccine keeps unclear. In this article, we report that hBD2 not only promoted the activation and maturation of immature dendritic cells (iDCs) by increasing MHC II and CD86 expression, but it also significantly upregulated the mRNA level of IL-6 and IL-12B in mouse bone marrow-derived dendritic cells. The serum concentrations of IFN-γ in mice stimulated with 300 ng hBD2 increased from 25.21 to 42.04 pg/mL, with a time extension from 4 to 12 h post-injection. During the process of three times immunization (1, 14, 28 days) with 3 μg hepatitis B vaccine combined with or without 300 ng hBD2 with a 2 week interval in BALB/c mice, the antibody against HBsAg (HBsAb) concentration in serum at every time point of observation in the combined group was statistically higher than the hepatitis B vaccine group. The serum concentration of IgG2a subclass HBsAb on the 14th day post last injection in the combined group was significantly higher than the hepatitis B vaccine group. Further, the splenic cells from the mice treated with both hBD2 and hepatitis B vaccine possessed a greater ability to produce a surface antigen of hepatitis B virus (HBsAg) specific IFN-γ than those treated with hepatitis B vaccine alone. The percentages of CD3+/CD4+ T cells and CD3+/CD8+ T lymphocytes in spleens from the mice treated with 300 ng hBD2 were statistically higher than the phosphate buffered saline group. These data suggest that hBD2 improves iDC maturation and the immune efficiency of hepatitis B vaccine in BALB/c mice.


Viral Immunol


Viral immunology


Wang M,Zeng W,Zhang Z,Zhang W,Su H,Zhang Z,Jiang L,Liu Y,Shi Q




Has Abstract


2020-12-28 00:00:00






  • Interleukin-10, interleukin-12, and interferon-gamma levels in the respiratory tract following mycoplasma hyopneumoniae and PRRSV infection in pigs.

    abstract::The cytokine profile associated with either a T helper 1 (Th1) or Th2 response in a porcine respiratory disease model was assessed by measuring IL-12, IL-10 and IFN-gamma using RT-PCR and ELISA, respectively. IL-10, IL-12, and IFN-gamma levels in pulmonary alveolar macrophages and bronchial lavage fluid were increased...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Thanawongnuwech R,Thacker EL

    更新日期:2003-01-01 00:00:00

  • Highly active antiretroviral therapy in patients infected with human immunodeficiency virus increases CD40 ligand expression and IL-12 production in cells ex vivo.

    abstract::Highly active anti-retroviral therapy (HAART) restores CD4(+) T-cell numbers in the periphery; however, its efficacy in restoring functional immunity is not fully elucidated. Here we evaluated longitudinal changes in the expression of several key markers of T-cell activation, namely CD40 ligand (CD154), OX40 (CD134), ...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Nyakeriga AM,Ying J,Shire NJ,Fichtenbaum CJ,Chougnet CA

    更新日期:2011-08-01 00:00:00

  • Comparison of antibody- and cell-mediated immune responses after intramuscular hepatitis C immunizations of BALB/c mice.

    abstract::Current treatments for hepatitis C infection have limited efficacy, and there is no vaccine available. The goal of this study was to compare the immune response to several immunization combinations against hepatitis C virus (HCV). Six groups of mice were immunized at weeks 0, 4, and 8 with different combinations of a ...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Ghorbani M,Nass T,Azizi A,Soare C,Aucoin S,Giulivi A,Anderson DE,Diaz-Mitoma F

    更新日期:2005-01-01 00:00:00

  • IFN-γ Attenuates Spontaneous Lymphocyte Proliferation by Fuelling Regulatory T Cells in HIV-1-Infected Patients.

    abstract::HIV infection is characterized by a high degree of immune activation. It has an impact on CD4 cell count and populations' distribution and function. T regulatory cells (Tregs) were found to play a controversial role in the course of infection because of their beneficial effect on the degree of immune activation and un...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Terzieva VI,Popova DN,Elenkov II

    更新日期:2017-04-01 00:00:00

  • Immune response to Fc tagged GP5 glycoproteins of porcine reproductive and respiratory syndrome virus.

    abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) glycoprotein 5 (GP5) is the most abundant envelope glycoprotein and a key target for neutralizing antibodies. Previous studies have demonstrated that the native GP5 glycoprotein is poorly immunogenic and not able to induce robust protective responses, probabl...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Wu F,Peng K,Tian J,Xu X,Zhou E,Chen H

    更新日期:2014-09-01 00:00:00

  • Double-Stranded Ribonucleic Acid-Mediated Antiviral Response Against Low Pathogenic Avian Influenza Virus Infection.

    abstract::Toll-like receptor (TLR)3 signaling pathway is known to induce type 1 interferons (IFNs) and proinflammatory mediators leading to antiviral response against many viral infections. Double-stranded ribonucleic acid (dsRNA) has been shown to act as a ligand for TLR3 and, as such, has been a focus as a potential antiviral...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Ahmed-Hassan H,Abdul-Cader MS,Ahmed Sabry M,Hamza E,Sharif S,Nagy E,Abdul-Careem MF

    更新日期:2018-07-01 00:00:00

  • Purification and partial characterization of a soluble suppressor factor from papillomavirus-infected patients.

    abstract::A molecular form of soluble suppressor factor was identified in serum-free supernatant fluid of cultured peripheral blood mononuclear cells (PBMC) from patients with severe forms of papillomavirus infections (epidermodysplasia verruciformis and large treatment-resistant condyloma acuminatum). The papillomavirus-induce...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Chopra V,Chopra AK,Tyring S

    更新日期:1991-01-01 00:00:00

  • Persistent effect of IFNAR-1 genetic polymorphism on the long-term pathogenesis of chronic HBV infection.

    abstract::Genetic polymorphism of IFNAR-1 plays a large role in determining the clearance or chronicity after hepatitis B virus (HBV) exposure. However, it is not clear whether type I interferon receptor-1 (IFNAR-1) variations continuously exert their effects to influence the final outcomes following HBV chronicity, including a...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: He XX,Chang Y,Jiang HJ,Tang F,Meng FY,Xie QH,Li PY,Song YH,Lin JS

    更新日期:2010-06-01 00:00:00

  • Clonal analysis of the cytotoxic T-lymphocyte response to reovirus.

    abstract::Reovirus has previously been classified into three serotypes based on hemagglutination inhibition assays. In the present study, the specificity of cytotoxic T lymphocytes (CTL) generated in reovirus type 3-infected C3H/HeN mice was investigated at the population and clonal levels. Short-term CTL lines generated in res...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Hogan KT,Cashdollar LW

    更新日期:1991-10-01 00:00:00

  • Delayed administration of interleukin-12 is efficacious in promoting recovery from lethal viral encephalitis.

    abstract::Vesicular stomatitis virus (VSV) applied intranasally to mice initially infects the olfactory receptor neurons, and then spreads quickly to the rest of the central nervous system (CNS). Previously, we have shown that the cytokine interleukin-12 (IL-12) has a significant survival and recovery promoting effect in mice i...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Ireland DD,Bang T,Komatsu T,Reiss CS

    更新日期:1999-01-01 00:00:00

  • Replicon Particle Expressing the E2 Glycoprotein of Bovine Viral Diarrhea Virus Immunization and Evaluation of Antibody Response.

    abstract::The objective of this study was to develop a new antigen delivery system using an alphavirus replicon particle (RP) to induce humoral antibody responses against bovine viral diarrhea virus (BVDV) recombinant antigen produced from envelope glycoprotein E2. An alphavirus RP expressing the E2 glycoprotein of BVDV was use...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Hossain MM,Rowland RR

    更新日期:2018-01-01 00:00:00

  • A small animal model for mother-to-fetus transmission of ts1, a murine retrovirus.

    abstract::Infection with a murine retrovirus, MoMuLV-TB, ts1 in BALB/c mice has been established as a small animal model for retroviral neurodegenerative disease as shown with infections such as HIV. However, mother-to-pup transmission has never been demonstrated in this model. The current investigation examines vertical transm...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Chakraborty J,Clark S,Okonta H,Duggan J

    更新日期:2003-01-01 00:00:00

  • Dendritic cell-based vaccine strategy against human immunodeficiency virus clade C: skewing the immune response toward a helper T cell type 2 profile.

    abstract::Given the continued spread of human immunodeficiency virus (HIV)-1 worldwide, developing efficient vaccine strategies against HIV-1 is a key task. We tested the safety and immunogenicity of a multicomponent, cell-based vaccine that consisted of antigen-expressing apoptotic bodies with or without autologous dendritic c...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Gruber A,Chalmers AS,Rasmussen RA,Ong H,Popov S,Andersen J,Hu SL,Ruprecht RM

    更新日期:2007-04-01 00:00:00

  • The prevalence of hepatitis B seropositivity among patients with cholangiocarcinoma.

    abstract::Cholangiocarcinoma is a malignancy of the biliary duct system. Presently, these malignant biliary tract diseases are common among the people in Southeast Asia, with the world's highest prevalence in northeastern Thailand and Laos. This carcinoma tends to grow slowly and to infiltrate the walls of the ducts, dissecting...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Pinyosophon A,Wiwanitkit V

    更新日期:2002-01-01 00:00:00

  • Immunization with a Recombinant Expression Vector Encoding NS3/NS4A of Hepatitis C Virus Genotype 3a Elicits Cell-Mediated Immune Responses in C57BL/6 Mice.

    abstract::Today, hepatitis C virus (HCV) infection is considered as one of the most significant international health concerns. Although novel therapeutic regimens against the infection have shown satisfactory results, no approved vaccine exists yet. This study aimed to evaluate the immunogenicity of a DNA vaccine candidate for ...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Behzadi MA,Alborzi A,Kalani M,Pouladfar G,Dianatpour M,Ziyaeyan M

    更新日期:2016-04-01 00:00:00

  • Anti-idiotypic antibodies to feline leukemia virus: an approach for retroviral immunization strategies.

    abstract::The present study describes an approach to the development and use of anti-idiotypic antibodies as a possible immunization strategy to prevent retroviral infection. The rationale for using anti-idiotypes (anti-Ids) to try to elicit an antigenic-specific immune response is examined, and the production and characterizat...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Tavares L,Roneker C,Postie L,Fevereiro M,de Noronha F

    更新日期:1991-04-01 00:00:00

  • Identification of an immunodominant peptide in the parvovirus B19 VP1 unique region able to elicit a long-lasting immune response in humans.

    abstract::The immune response against parvovirus B19 is mainly directed against the two structural proteins, VP1 and VP2. The amino terminal half of the VP1 unique region has been shown to elicit a dominant immune response in humans, more effective than other linear epitopes and also it has been seen to contain significant neut...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Zuffi E,Manaresi E,Gallinella G,Gentilomi GA,Venturoli S,Zerbini M,Musiani M

    更新日期:2001-01-01 00:00:00

  • Mannan-binding lectin (MBL) serum concentration in relation to propagation of infectious bronchitis virus (IBV) in chickens.

    abstract::Mannan-binding lectin (MBL) is a collectin that mediates activation of the complement system and is of importance for host defenses. In humans low concentrations of MBL in serum have been associated with susceptibility to several viral diseases. To understand the function of MBL in relation to infectious viral disease...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Juul-Madsen HR,Norup LR,Handberg KJ,Jørgensen PH

    更新日期:2007-12-01 00:00:00

  • Effects of in vivo depletion of immunocyte populations on herpes simplex virus glycoprotein D vaccine-induced resistance to HSV2 challenge.

    abstract::BALB/c mice, preimmunized with a protective dose of native herpes simplex virus type 1 glycoprotein D (ngD1) vaccine, were depleted of selected immunocyte populations in vivo using monoclonal antibodies directed at Thy1+, L3T4+, or Lyt2+ cells. Following immunization and depletion, animals were inoculated with varied ...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Mishkin EM,Blasiak M,Giorgio D,Ishizaka ST

    更新日期:1992-07-01 00:00:00

  • Opinion: Making Inactivated and Subunit-Based Vaccines Work.

    abstract::Empirically derived vaccines have in the past relied on the isolation and growth of disease-causing microorganisms that are then inactivated or attenuated before being administered. This is often done without prior knowledge of the mechanisms involved in conferring protective immunity. Recent advances in scientific te...

    journal_title:Viral immunology

    pub_type: 杂志文章,评审


    authors: Chua BY,Sekiya T,Jackson DC

    更新日期:2018-03-01 00:00:00

  • A recombinant flagellin-poxvirus fusion protein vaccine elicits complement-dependent protection against respiratory challenge with vaccinia virus in mice.

    abstract::Bacterial flagellin is a potent adjuvant that enhances adaptive immune responses to a variety of protein antigens. The vaccinia virus antigens L1R and B5R are highly immunogenic in the context of the parent virus, but recombinant forms of the proteins are only weakly immunogenic. Therefore we evaluated the humoral res...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Delaney KN,Phipps JP,Johnson JB,Mizel SB

    更新日期:2010-04-01 00:00:00

  • Pandemic H1N1 influenza A virus induces a potent innate immune response in human chorionic cells.

    abstract::To understand the mechanistic basis for the reported outcomes of influenza A virus (IAV) infection during pregnancy, the effects of mouse adapted and pandemic (pdm) IAV infection in human choriocarcinoma cells were examined. Both viruses were able to infect and replicate in human placental cells, with pdm IAV being mo...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Kumar SR,Biswas M,Elankumaran S

    更新日期:2014-04-01 00:00:00

  • Development of a Time and Cost Benefit Antibody Binding Test-Based Method for Determination of Rabies Vaccine Potency.

    abstract::This study is an improvement on the antibody binding test, known as ABT method, to develop a simple and fast method in comparison with NIH for determination of rabies vaccine potency. In the current study, several commercial human and veterinary vaccines were tested using both modified ABT and NIH methods. The ED50 wa...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Asgary V,Mojtabavi N,Janani A,Mousavi T,Hadjati J,Khosravy MS,Ahangari Cohan R

    更新日期:2017-04-01 00:00:00

  • gamma-Herpesvirus-induced protection against bacterial infection is transient.

    abstract::Herpesviruses are widely disseminated in the population and establish lifelong latency, which is associated with a variety of pathological consequences. A recent report showed that mice latently infected with either murine gamma-herpesvirus-68 (gammaHV68) or murine cytomegalovirus (mCMV), mouse pathogens genetically s...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Yager EJ,Szaba FM,Kummer LW,Lanzer KG,Burkum CE,Smiley ST,Blackman MA

    更新日期:2009-02-01 00:00:00

  • Cellular gene expression profiles in rhesus macaques challenged mucosally with a pathogenic R5 tropic simian human immunodeficiency virus isolate.

    abstract::Insights into the host factors that contribute to an effective antiviral immune response may be obtained by examining global gene expression in simian human immunodeficiency virus (SHIV)-infected nonhuman primates that exhibit different virological outcomes. Immune responses and gene expression profiles in peripheral ...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Chung HK,Pise-Masison CA,Radonovich MF,Brady J,Lee JK,Cheon SY,Markham P,Cristillo A,Pal R

    更新日期:2008-12-01 00:00:00

  • Host evasion by emerging paramyxoviruses: Hendra virus and Nipah virus v proteins inhibit interferon signaling.

    abstract::Interferon (IFN) can activate Signal Transducer and Activator of Transcription (STAT) proteins to establish a cellular antiviral response and inhibit virus replication. Many viruses have evolved strategies to inhibit this antiviral mechanism, but paramyxoviruses are unique in their abilities to directly target the IFN...

    journal_title:Viral immunology

    pub_type: 杂志文章,评审


    authors: Rodriguez JJ,Horvath CM

    更新日期:2004-01-01 00:00:00

  • Loss of RORγt DNA binding activity inhibits IL-17 expression in HIV-1 infected Indian individuals.

    abstract::Abstract IL-17 producing CD4 T cells have recently been shown to play an important role in mucosal immunity in HIV infection. But its role in peripheral immunity and the molecular mechanism underlying its regulation during HIV-1 infection are ill defined. In this study, we report a significant negative correlation b...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Singh A,Vajpayee M,Ali SA,Chauhan NK

    更新日期:2013-02-01 00:00:00

  • A decrease of regulatory T cells and altered expression of NK receptors are observed in subacute sclerosing panencephalitis.

    abstract::Subacute sclerosing panencephalitis (SSPE) is caused by a persistent measles virus infection. Regulatory mechanisms can be responsible for a failure of immunosurveillance in children with SSPE. In this study, peripheral blood cells of 71 patients with SSPE and 57 children with other diseases were compared phenotypical...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Yentur SP,Gurses C,Demirbilek V,Adin-Cinar S,Kuru U,Uysal S,Yapici Z,Yilmaz G,Cokar O,Onal E,Gökyigit A,Saruhan-Direskeneli G

    更新日期:2014-12-01 00:00:00

  • Evaluating Diagnostic Accuracy of Noninvasive Tests in Assessment of Significant Liver Fibrosis in Chronic Hepatitis C Egyptian Patients.

    abstract::Stage of liver fibrosis is critical for treatment decision and prediction of outcomes in chronic hepatitis C (CHC) patients. We evaluated the diagnostic accuracy of transient elastography (TE)-FibroScan and noninvasive serum markers tests in the assessment of liver fibrosis in CHC patients, in reference to liver biops...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Omran D,Zayed RA,Nabeel MM,Mobarak L,Zakaria Z,Farid A,Hassany M,Saif S,Mostafa M,Saad OK,Yosry A

    更新日期:2018-05-01 00:00:00

  • PyNTTTTGT prototype oligonucleotide IMT504, a novel effective adjuvant of the FMDV DNA vaccine.

    abstract::Synthetic oligodeoxynucleotides (ODNs) such as CpG can stimulate B and plasmacytoid dendritic cells in vertebrate immune systems. Several studies showed that non-CpG ODNs could also induce strong stimulation of B and T cells. PyNTTTTGT ODNs, non-CpG ODNs, can activate and cause immunoglobulin secretion by B cells and ...

    journal_title:Viral immunology

    pub_type: 杂志文章


    authors: Zhao G,Jin H,Li J,Su B,Du X,Kang Y,Wang X,Wang B

    更新日期:2009-04-01 00:00:00