Sunflower metallothionein family characterisation. Study of the Zn(II)- and Cd(II)-binding abilities of the HaMT1 and HaMT2 isoforms.


:Plant metallothioneins (MTs) constitute a family of small Cys-rich proteins capable of coordinating metal ions, significantly differing from microbial and animal MTs. They are divided into four subfamilies depending on the Cys pattern in their sequence. In this work, the MT system of the sunflower plant (Helianthus annuus) has been defined, with ten genes coding for MTs (HaMT) belonging to the four plant MT subfamilies; three HaMT1, four HaMT2, one HaMT3 and two HaMT4 isoforms. The gene expression pattern and capacity to confer metal resistance to yeast cells have been analysed for at least one member of each subfamily. The divalent metal ion-binding abilities of HaMT1-2 and HaMT2-1 (the isoforms encoded by the most abundantly expressed HaMT1 and HaMT2 isogenes) have been characterised, as HaMT3 and HaMT4 were previously studied. Those isoforms constitute an optimum material to study the effect of Cys number variability on their coordination abilities, as they exhibit additional Cys residues regarding the canonical Cys pattern of each subfamily. Our results show that the variation in the number of Cys does not drastically modify their M(II)-binding abilities, but instead modulates the degree of heterogeneity of the corresponding recombinant syntheses. Significantly, the Zn(II)-HaMT1 complexes were highly susceptible to proteolytic cleavage. The recombinant Cd-MT preparations of both isoforms exhibit significant acid-labile sulphide content-Cd6S8 or Cd7S7 species. Overall results suggest that HaMT2-1 is probably associated with Cd(II) detoxification, in contrast to HaMT1-2, which may be more related to physiological functions, such as metal ion transport and delivery.


J Inorg Biochem


Tomas M,Pagani MA,Andreo CS,Capdevila M,Atrian S,Bofill R




Has Abstract


2015-07-01 00:00:00












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    journal_title:Journal of inorganic biochemistry

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    authors: Alessio M,Zanellato I,Bonarrigo I,Gabano E,Ravera M,Osella D

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  • Activation of monoamine oxidase isotypes by prolonged intake of aluminum in rat brain.

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    authors: Huh JW,Choi MM,Lee JH,Yang SJ,Kim MJ,Choi J,Lee KH,Lee JE,Cho SW

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    authors: Krzywoszyńska K,Swiątek-Kozłowska J,Potocki S,Ostrowska M,Kozłowski H

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    authors: Leavis PC,Evans JS,Levine BA

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    authors: Suwalsky M,Norris B,Cárdenas H

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    authors: Meng XG,Guo Y,Hu CW,Zeng XC

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  • Heavy metal-regulated new microRNAs from rice.

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    更新日期:2009-02-01 00:00:00

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    authors: Masala O,O'Brien P,Rainbow PS

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    journal_title:Journal of inorganic biochemistry

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    authors: Pin Y,Zhang XP

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    journal_title:Journal of inorganic biochemistry

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    authors: Ruiz J,Rodríguez V,Cutillas N,Espinosa A,Hannon MJ

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章


    authors: Rebolledo AP,Vieites M,Gambino D,Piro OE,Castellano EE,Zani CL,Souza-Fagundes EM,Teixeira LR,Batista AA,Beraldo H

    更新日期:2005-03-01 00:00:00

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    pub_type: 杂志文章


    authors: Gómez-Ruiz S,Kaluderović GN,Prashar S,Hey-Hawkins E,Erić A,Zizak Z,Juranić ZD

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    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章


    authors: Nayak KK,Bhattacharyya R,Maity P

    更新日期:1991-03-01 00:00:00

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    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章


    authors: Yuan Q,Cai K,Qi ZP,Bai ZS,Su Z,Sun WY

    更新日期:2009-08-01 00:00:00

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    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章


    authors: Siegbahn PE,Lundberg M

    更新日期:2006-05-01 00:00:00

  • Preparation, characterization, and antitumor activity of new ethylenediamine platinum(IV) complexes containing mixed carboxylate ligands.

    abstract::A series of ethylenediamine platinum(IV) complexes of the type PtIV(en)XA2 and PtIV(en)X'2A2, where X = 1,1-cyclobutanedicarboxylato or malonato, X' = chloro, cyclobutanecarboxylato, cyclopentanecarboxylato, or cyclohexanecarboxylato, and A = acetato or trifluoroacetato were synthesized and characterized by elemental ...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章


    authors: Khokhar AR,Deng Y,Kido Y,Siddik ZH

    更新日期:1993-05-01 00:00:00