MicroRNA-26a overexpression protects RGC-5 cells against H2O2-induced apoptosis.


BACKGROUND:We intended to examine the functional role of microRNA 26 (miR-26a) in regulating H2O2-induced cytotoxicity and apoptosis in RGC-5 cells in vitro. METHOD:Various concentrations of H2O2 (0-1000 μM) were added in RGC-5 culture. Cell cytotoxicity was monitored by viability assay and gene expression level of miR-26a examined by qRT-PCR. MicroRNA-26a mimic was then applied in the RGC-5 culture to examine its effect on upregulating endogenous miR-26a and rescuing H2O2-induced cytotoxicity. TUNEL immunostaining assay was used to further assess the protective effect of upregulating miR-26a on H2O2-induced apoptosis in RGC-5 cells. Direct targeting of miR-26a on Phosphatase and tensin homolog (PTEN) signaling pathway was assessed by luciferase assay and western blotting. PTEN was then ectopically over-expressed in RGC-5. And its effects on miR-26a mediated apoptosis protection in RGC-5 were investigated by western blot and TUNEL assay. RESULTS:H2O2 induced cytotoxicity and down-regulated miR-26a in dose-dependent manner in RGC-5 cells. MiR-26a-mimic upregulated endogenous miR-26a gene levels, and then reduced H2O2-induced cytotoxicity, as well as H2O2-induced apoptosis in RGC-5 cells. PTEN was directly targeted by miR-26a. PTEN protein was upregulated, and phosphorylated AKT protein down-regulated while miR-26a was upregulated to reduce H2O2-induced apoptosis. Finally, overexpressing PTEN reversed the protective effect of miR-26a upregulation on RGC-5 apoptosis. CONCLUSION:Upregulating miR-26a protects RGC-5 cell against cytotoxicity and apoptosis, probably through down-regulation of PTEN.


Kang Y,Jia P,Zhao H,Hu C,Yang X




Has Abstract


2015-05-01 00:00:00














  • Abnormal signalling of 14-3-3 proteins in cells with accumulated xanthurenic acid.

    abstract::Xanthurenic acid is an endogenous molecule leading to caspase-9 and -3 activation. Here we report that xanthurenic acid targets signalling proteins 14-3-3 into lysosomes leading to interruption protein/protein interaction. Xanthurenic acid changed the localisation of 14-3-3 in the cells. At a concentration of 10 and 2...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Malina HZ,Frueh BE

    更新日期:2003-10-17 00:00:00

  • Calcium effects on prothrombin and its reaction with bifunctional alkylating reagents.

    abstract::Monodisperse bovine prothrombin was prepared and its molecular states under several conditions examined. The protein showed no tendency to self-associate in the absence of calcium. Calcium (4 mM) caused small increases in the apparent molecular weight of the protein which may or may not represent protein dimerization ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Silversmith RE,Wei GJ,Nelsestuen GL

    更新日期:1983-02-28 00:00:00

  • ERRF is essential for Estrogen-Estrogen Receptor alpha signaling pathway in ER positive breast cancer cells.

    abstract::Estrogen-Estrogen Receptor alpha (ERα) belongs to one of the most important signaling pathways controlling breast tissue development and progression of breast cancer. ERRF was recently identified as a candidate breast cancer associated protein and showed positive association with ERα status in clinical samples and cel...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Luo A,Zhang X

    更新日期:2016-05-27 00:00:00

  • High mobility group A2 (HMGA2) promotes EMT via MAPK pathway in prostate cancer.

    abstract::Studies have shown that High mobility group A2 (HMGA2), a non-histone protein, can promote epithelial-mesenchymal transition (EMT), which plays a critical role in prostate cancer progression and metastasis. Interestingly, full-length or wild-type HMGA2 and truncated (lacking the 3'UTR) HMGA2 isoforms are overexpressed...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hawsawi O,Henderson V,Burton LJ,Dougan J,Nagappan P,Odero-Marah V

    更新日期:2018-09-26 00:00:00

  • Preparation and partial characterization of polyclonal antibodies raised against a preselected sequence of calcium dependent lectins.

    abstract::The synthetic-peptide strategy was used to generate antibodies raised against calcium-dependent lectins of vertebrates. We demonstrate that a synthetic peptide predicted from the amino acid sequence of the carbohydrate recognition domain can induce blocking antibodies which would react with, or in close vicinity of, t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Blanck O,Thibault V,Granier C,Van Rietschoten J,Courageot J,Miquelis R

    更新日期:1990-06-29 00:00:00

  • RAP80 binds p32 to preserve the functional integrity of mitochondria.

    abstract::RAP80, a member of the BRCA1-A complex, is a well-known crucial regulator of cell cycle checkpoint and DNA damage repair in the nucleus. However, it is still unclear whether Rap80 localizes to another region outside the nucleus and plays different roles with its partners. Here, we found mitochondrial p32 as a novel bi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Chung HJ,Korm S,Lee SI,Phorl S,Noh S,Han M,Naskar R,Kim H,Lee JY

    更新日期:2017-10-21 00:00:00

  • Fcf1p and Fcf2p are novel nucleolar Saccharomyces cerevisiae proteins involved in pre-rRNA processing.

    abstract::The uncharacterized Saccharomyces cerevisiae proteins Fcf1 and Fcf2, encoded by the ORFs YDR339c and YLR051c, respectively, were identified in a tandem affinity purification experiment of the known 40S factor Faf1p. Most of the proteins associated with TAP-Faf1p are trans-acting factors involved in pre-rRNA processing...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Rempola B,Karkusiewicz I,Piekarska I,Rytka J

    更新日期:2006-07-28 00:00:00

  • The glycine analogue, aminomethanesulfonic acid, inhibits LPS-induced production of TNF-alpha in isolated rat Kupffer cells and exerts hepatoprotective effects in mice.

    abstract::The activation of Kupffer cells represents a central mechanism of liver injury involving the production of TNF-alpha. It is known that glycine prevents LPS-induced production of TNF-alpha in isolated Kupffer cells. In this study, the possibility that glycine analogues might affect Kupffer cells was investigated. As a ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ishizaki-Koizumi S,Sonaka I,Takei Y,Ikejima K,Sato N

    更新日期:2004-09-17 00:00:00

  • TRAF-dependent association of protein kinase Tpl2/COT1 (MAP3K8) with CD40.

    abstract::Signaling by TNF-family receptor CD40 involves TRAF-family adaptor proteins, leading to activation of protein kinases that induce NFkappaB-family transcription factors. We report here that mitogen activated protein kinase kinase kinase-8 (MAP3K8), Tpl2/COT1, is recruited to the CD40 complex via a mechanism dependent o...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Chan H,Reed JC

    更新日期:2005-03-04 00:00:00

  • Dickkopf-1 induced apoptosis in human placental choriocarcinoma is independent of canonical Wnt signaling.

    abstract::Placental choriocarcinoma, a reproductive system carcinoma in women, has about 0.81% occurrence frequency in China, which leads to over 90% lethality due to indistinct pathogenesis and the absence of efficient therapeutic treatment. In the present study, using immunostaining and reverse transcription PCR, we reported ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Peng S,Miao C,Li J,Fan X,Cao Y,Duan E

    更新日期:2006-11-24 00:00:00

  • Identification of early gene expression changes in primary cultured neurons treated with topoisomerase I poisons.

    abstract::Topoisomerase 1 (TOP1) poisons like camptothecin (CPT) are currently used in cancer chemotherapy but these compounds can have damaging, off-target effects on neurons leading to cognitive, sensory and motor deficits. To understand the molecular basis for the enhanced sensitivity of neurons to CPT, we examined the effec...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Rossi SL,Lumpkin CJ,Harris AW,Holbrook J,Gentillon C,McCahan SM,Wang W,Butchbach MER

    更新日期:2016-10-14 00:00:00

  • F0F1-ATPase of plant mitochondria: isolation and polypeptide composition.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hamasur B,Glaser E

    更新日期:1990-08-16 00:00:00

  • Irigenin sensitizes TRAIL-induced apoptosis via enhancing pro-apoptotic molecules in gastric cancer cells.

    abstract::Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) holds promising value for cancer therapy due to its capacity to induce apoptosis in cancer cells. Nevertheless, TRAIL therapy is greatly hampered by its resistance. Irigenin (Iri), isoflavonoids, can be isolated from the rhizome of Belamcanda chinensis, a...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Xu Y,Gao CC,Pan ZG,Zhou CW

    更新日期:2018-02-12 00:00:00

  • Platelet surface membranes are highly mitogenic for coronary artery smooth muscle cells--A novel mechanism for sustained proliferation after vessel injury?

    abstract::The effects of isolated platelet surface membranes on DNA synthesis and proliferation of bovine coronary artery smooth muscle cells (SMC) were studied. Platelet membranes were very potent mitogens for SMC. The potency was about 10-fold higher than the maximum effects of platelet-derived growth factor-BB (PDGF). Platel...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Weber AA,Zucker TP,Schrör K

    更新日期:1999-06-07 00:00:00

  • Isoprenylation of the low molecular mass GTP-binding proteins rac 1 and rac 2: possible role in membrane localization.

    abstract::Ras proteins can be modified at their COOH-terminal cysteine in the motif Cys-Ali-Ali-Xaa by a farnesyl isoprenoid. This modification is essential for membrane association and biological activity of ras proteins. A similar COOH-terminal amino acid sequence, Cys-Xaa-Ali-Xaa, exists in the ras-related GTP-binding protei...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Didsbury JR,Uhing RJ,Snyderman R

    更新日期:1990-09-14 00:00:00

  • Plant sterols in food: no consensus in guidelines.

    abstract::Plant sterols are supplemented in foods to reduce cardiovascular risk. Randomized controlled trials show 2 g of plant sterols a day reduce serum cholesterol by about 10%. This reduction in serum cholesterol levels is achieved at the expense of increased serum plant sterol levels. Findings in patients with phytosterole...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章,评审


    authors: Weingärtner O,Baber R,Teupser D

    更新日期:2014-04-11 00:00:00

  • A cytoplasmic nickel-iron hydrogenase with high specific activity from Desulfovibrio multispirans sp. N., a new species of sulfate reducing bacterium.

    abstract::A hydrogenase from a new species of sulfate reducing bacterium has been isolated and characterized. In contrast to other hydrogenases isolated from Desulfovibrio, this enzyme is found in the cytoplasmic fraction rather than in the periplasm. The specific activity of the enzyme, as measured in the hydrogen evolution as...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Czechowski MH,He SH,Nacro M,DerVartanian DV,Peck HD Jr,LeGall J

    更新日期:1984-12-28 00:00:00

  • An ND-6 mitochondrial DNA mutation associated with Leber hereditary optic neuropathy.

    abstract::A mitochondrial DNA mutation at nucleotide position 14,484 was found in 14 independent probands with Leber hereditary optic neuropathy and in 0/250 controls. The 14,484 mutation, which changes methionine-64 to valine in a conserved domain of the ND-6 gene, occurred in association with a mitochondrial DNA haplotype tha...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Johns DR,Neufeld MJ,Park RD

    更新日期:1992-09-30 00:00:00

  • Calcium is required for the mitogenic activation of lymphocytes by periodic acid oxidation.

    abstract::This investigation of Ca2+ requirements for the mitogenic activation of lymphocytes by periodic acid has shown that oxidation by periodate causes an immediate and transient increase of Ca2+ influx and efflux in oxidized cells. Oxidized lymphocytes maintained in the medium containing 0.2 mM Ca2+ failed to proliferate o...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hornicek FJ,Malinin GI,Malinin TI

    更新日期:1992-02-28 00:00:00

  • T-kininogen--the major plasma kininogen in rat adjuvant arthritis.

    abstract::Total kininogen in plasma of Freund's adjuvant treated rats increased 20-fold 7 days following the injection. Analysis of the kininogens demonstrated that increases in T-kininogen was the major reason for the rise in kininogen. High molecular weight and low molecular weight kininogens showed little or no change. The i...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Barlas A,Okamoto H,Greenbaum LM

    更新日期:1985-05-31 00:00:00

  • Direct radioimmunoassay of atrial natriuretic factor.

    abstract::A direct radioimmunoassay of atrial natriuretic factor (ANF) has been developed. The method uses a synthetic 26 amino-acid fragment (8-33 ANF) of the native peptide. Antibodies have been prepared in rabbits immunized with the peptide coupled to thyroglobulin. The radiolabelled tracer prepared by iodination according t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Gutkowska J,Thibault G,Januszewicz P,Cantin M,Genest J

    更新日期:1984-07-31 00:00:00

  • Cloning and sequence analysis of a cDNA encoding tetrameric carbonyl reductase of pig lung.

    abstract::A cDNA for tetrameric carbonyl reductase was cloned from a pig lung cDNA library. The cDNA had an open reading frame encoding a protein of 244 amino acid residues with a predicted monomer molecular weight of 25,985. The authenticity of the cDNA was confirmed by a perfect match of 80 amino acids determined from sequenc...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Nakanishi M,Deyashiki Y,Nakayama T,Sato K,Hara A

    更新日期:1993-08-16 00:00:00

  • Specific binding of plasminogen to vitronectin. Evidence for a modulatory role of vitronectin on fibrin(ogen)-induced plasmin formation by tissue plasminogen activator.

    abstract::Vitronectin immobilized onto polystyrene microtiter wells was demonstrated to specifically bind plasminogen in a concentration-dependent manner, yielding an estimated KD = 0.4 microM. Heparin only moderately interfered with the vitronectin-plasminogen interaction, whereas high concentrations of 6-amino-hexanoic acid i...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Preissner KT

    更新日期:1990-05-16 00:00:00

  • Inhibition of arachidonate lipoxygenase12 targets lung cancer through inhibiting EMT and suppressing RhoA and NF-κB activity.

    abstract::The carcinogenic function of arachidonate lipoxygenase12 (Alox12) has been reported in various cancers. However, little is known on the role of Alox12 in lung cancer. Here, we demonstrate that Alox12 is upregulated and contributes to biological activities of lung cancer through multiple mechanisms. We found that Alox1...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Chen J,Tong W,Liao M,Chen D

    更新日期:2020-04-16 00:00:00

  • Long noncoding RNA UCA1 promotes chondrogenic differentiation of human bone marrow mesenchymal stem cells via miRNA-145-5p/SMAD5 and miRNA-124-3p/SMAD4 axis.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Shu T,He L,Wang X,Pang M,Yang B,Feng F,Wu Z,Liu C,Zhang S,Liu B,Wang Q,Rong L

    更新日期:2019-06-18 00:00:00

  • Anabolic function of the type II isozyme of hexokinase in hepatic lipid synthesis.

    abstract::The mRNA encoding the Type II isozyme of hexokinase was markedly elevated in livers of transgenic mice overexpressing the transcriptionally active nuclear form of sterol regulatory element binding protein-1a (nSREBP-1a), but not in transgenic mice overexpressing the nSREBP-1c or nSREBP-2 isoforms. Cellulose acetate el...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sebastian S,Horton JD,Wilson JE

    更新日期:2000-04-21 00:00:00

  • Molecular docking of inhibitors into monoamine oxidase B.

    abstract::Monoamine oxidase B (MAO-B) functions in the deamination of monoamines, including dopamine and norepinephrine. The search for MAO-B inhibitors increased following the discovery that the enzyme may be responsible for generating neurotoxins from various endogenous or exogenous compounds. Computational screening methods ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Harkcom WT,Bevan DR

    更新日期:2007-08-24 00:00:00

  • Prosomatostatin II processing is initiated in the trans-Golgi network of anglerfish pancreatic cells.

    abstract::Anglerfish prosomatostatin II, the precursor of somatostatin-28 II, is produced in different cells from prosomatostatin I, by a cleavage at Arg73. Antibodies were raised against the carboxy-terminal [64-72] portion of the precursor II upstream from somatostatin-28 II sequence. These antibodies recognized only this epi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Bourdais J,Devilliers G,Girard R,Morel A,Benedetti L,Cohen P

    更新日期:1990-08-16 00:00:00

  • The ubiquitin-interacting motifs of S5a as a unique upstream inhibitor of the 26S proteasome.

    abstract::It has been demonstrated that ubiquitin-conjugated proteins were accumulated by ectopically-expressed S5a as well as the ubiquitin-interacting motifs of S5a (S5a-UIMs). In this study, we further found that free S5a-UIMs stabilized only a subset of proteasomal substrates including p53, c-Fos, c-Jun, and p27 but not bet...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Elangovan M,Shin DY,Yoo YJ

    更新日期:2009-10-30 00:00:00

  • Enhanced phosphatidylinositol 3-kinase activity and high phosphorylation state of its downstream signalling molecules mediated by ret with the MEN 2B mutation.

    abstract::We compared the intracellular signalling pathways through Ret tyrosine kinase activated by glial cell line-derived neurotrophic factor (GDNF), multiple endocrine neoplasia (MEN) 2A, or MEN 2B mutation. Tyrosine phosphorylation of Grb2-associated binder-1 (Gab1) and activation of phosphatidylinositol 3-kinase (PI 3-kin...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Murakami H,Iwashita T,Asai N,Shimono Y,Iwata Y,Kawai K,Takahashi M

    更新日期:1999-08-19 00:00:00