Interleukin-4 suppresses immunoglobulin production by peripheral blood lymphocytes of patients with common variable immunodeficiency (CVI) induced by supernatants of T cell clones.


:Supernatants of both CD4+ and CD8+ alloreactive T cell clones induced IgM, IgG and IgA synthesis by peripheral blood lymphocytes (PBL) of healthy donors in vitro. These supernatants were also tested on their capacity to induce immunoglobulin production by PBL of four patients with CVI and one patient with CVI and thymoma. A low degree of IgM, IgG and IgA production was induced in one patient with CVI. In the patient with CVI and thymoma, induction of IgG and IgA synthesis was in the normal range, whereas IgM production was reduced. In the three other patients only a low production of IgM was induced. Interestingly, pre-incubation of the PBL for 24 h with interleukin-4 (IL-4) suppressed immunoglobulin production both by PBL of the patients with CVI and healthy donors. The strongest inhibitory effects were observed on IgA synthesis. These data indicate that B cells of three patients with CVI can not be induced to switch to IgG or IgA producing cells in vitro. In contrast, B cells of the patient with CVI and thymoma were able to respond to the relevant B cell growth and differentiation factors present in the T cell clone supernatants, suggesting that the T cells of this patient may fail to produce these factors. However, the proliferative responses of the T cells to phytohaemagglutinin (PHA), concanavalin A (Con A) and pokeweed mitogen (PWM), were normal in all five patients tested. In addition, the interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) production by PBL of the five patients was also in the normal range. Although only a small number of patients was tested, these results support the view that defects in both regulatory T cell functions and/or intrinsic B cell defects may contribute to the pathogenesis of CVI.


Clin Exp Immunol


Pastorelli G,Roncarolo MG,Touraine JL,Rousset F,Pene J,de Vries JE


Has Abstract


1989-12-01 00:00:00












  • Responses of 'mature' human B lymphocyte lines to inducers of maturation and activation.

    abstract::Four human B cell lines with a mature phenotype (immunoglobulin secretion and expression of membrane markers associated with maturation) were cultured in the presence of phorbol ester (PMA), dimethyl sulphoxide (DMSO) and two conditioned media. PMA and DMSO led to changes in phenotype which suggested the cells were be...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Zola H,Griffith V,Hunter I,Koh L,Bradley J

    更新日期:1986-11-01 00:00:00

  • Diabetic sera react with the glutamic acid decarboxylase molecule in a dimeric-oligomeric form.

    abstract::Glutamic acid decarboxylase (GAD) is a major autoantigen in insulin-dependent diabetes mellitus (IDDM). This was initially identified as a 64-65 kD molecule according to migration in gels after immunoprecipitation from pancreatic islets. We studied the antigenicity of two different radiolabelled preparations of GAD, d...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Rowley MJ,Chen QY,Teoh KL,Zimmet PZ,Tuomi T,Knowles WJ,Mackay IR

    更新日期:1996-11-01 00:00:00

  • Dexamethasone impairs pulmonary defence against Pseudomonas aeruginosa through suppressing iNOS gene expression and peroxynitrite production in mice.

    abstract::To elucidate the in vivo mechanisms involved in the impairment in pulmonary defence as the result of treatment with glucocorticoids, we established fatal pneumonia with bacteraemia in dexamethasone (DEX)-treated mice by means of an intratracheal challenge of Pseudomonas aeruginosa. An increased neutrophil influx was o...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Satoh S,Oishi K,Iwagaki A,Senba M,Akaike T,Akiyama M,Mukaida N,Atsushima KM,Nagatake T

    更新日期:2001-11-01 00:00:00

  • Human neutrophil Fc receptor-mediated adhesion under flow: a hollow fibre model of intravascular arrest.

    abstract::Human polymorphonuclear cells (PMN) were found to adhere to a novel model of blood vessel wall-associated IgG. The internal surfaces of cellulose acetate hollow fibres, of comparable internal diameter to small blood vessels, were coated with normal serum human IgG, heat-aggregated IgG (HAIgG), laminin or fibrinogen. U...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: D'Arrigo C,Candal-Couto JJ,Greer M,Veale DJ,Woof JM

    更新日期:1995-04-01 00:00:00

  • Effect of plasmapheresis on T anc B lymphocyte functions in patients with systemic lupus erythematosus: a double blind study.

    abstract::Nine patients with active systemic lupus erythematosus entered a double-blind randomized trial to study the therapeutic effect of vigorous versus sham reinfusion plasmapheresis. Four of them received real plasmapheresis while five received sham reinfusion plasmapheresis. In the present communication we report the effe...

    journal_title:Clinical and experimental immunology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Tsokos GC,Balow JE,Huston DP,Wei N,Decker JL

    更新日期:1982-05-01 00:00:00

  • Circulating immune complexes in old people and in diabetics: correlation with autoantibodies.

    abstract::Circulating immune complexes (CIC) were detected by a solid-phase radioassay in 34% of fifty-three insulin-dependent diabetics (IDD) as compared to 18% of forty-five non-insulin-dependent diabetics (NIDD) and 14% of 173 control subjects. In control subjects, the prevalence of CIC increased with age and was higher in m...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Delespesse G,Gausset P,Sarfati M,Dubi-Rucquoy M,Debisschop MJ,van Haelst L

    更新日期:1980-04-01 00:00:00

  • 5,7-dihydroxy-3,4,6-trimethoxyflavone inhibits the inflammatory effects induced by Bacteroides fragilis enterotoxin via dissociating the complex of heat shock protein 90 and I kappaB alpha and I kappaB kinase-gamma in intestinal epithelial cell culture.

    abstract::Enterotoxin produced by enterotoxigenic Bacteroides fragilis (BFT) has been associated with mucosal inflammation and diarrhoeal diseases. In this study, the anti-inflammatory molecular mechanism of 5,7-dihydroxy-3,4,6-trimethoxyflavone (eupatilin) was characterized in an HT-29 intestinal epithelial cell line stimulate...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Kim JM,Lee DH,Kim JS,Lee JY,Park HG,Kim YJ,Oh YK,Jung HC,Kim SI

    更新日期:2009-03-01 00:00:00

  • Illegitimate RAG-mediated recombination events are involved in IKZF1 Δ3-6 deletion in BCR-ABL1 lymphoblastic leukaemia.

    abstract::Breakpoint cluster region-Abelson murine leukaemia viral oncogene homologue 1 (BCR-ABL1), encoded by the Philadelphia (Ph) chromosome, is the characteristic of chronic myeloid leukaemia (CML) and a subset of acute lymphoblastic leukaemia (ALL). We demonstrated that expression of the Ik6 transcript, which lacked exons ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Dong Y,Liu F,Wu C,Li S,Zhao X,Zhang P,Jiao J,Yu X,Ji Y,Zhang M

    更新日期:2016-09-01 00:00:00

  • Nuclear deposits of immunoglobulins in skin of patients with systemic lupus erythematosus.

    abstract::Skin nuclear speckled IgG deposition was noted in seven patients. The patients' clinical courses satisfied at least four of the preliminary criteria of the American Rheumatism Association for the diagnosis of systemic lupus erthematosus (SLE). Examination of approximately 700 additional biopsies from patiets with SLE,...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Shu S,Provost T,Croxdale MB,Reichlin M,Beutner EH

    更新日期:1977-02-01 00:00:00

  • Activation of c-Jun N-terminal kinase (JNK) signalling in experimentally induced gastric lesions in rats.

    abstract::The c-Jun N-terminal kinase (JNK) participates in intracellular signalling cascades that mediate inflammatory responses. Therefore, the JNK signalling may be involved in gastric injury and inhibition of this pathway may form the basis of a new strategy for the treatment of gastric injury. The aim of this study was to ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Mitsuyama K,Tsuruta O,Matsui Y,Harada K,Tomiyasu N,Suzuki A,Takaki K,Masuda J,Handa K,Satoh Y,Bennett BL,Toyonaga A,Sata M

    更新日期:2006-01-01 00:00:00

  • Predominant type-2 response in infants with respiratory syncytial virus (RSV) infection demonstrated by cytokine flow cytometry.

    abstract::Acute RSV infection in infancy may produce some asthma-like symptoms and may be followed by a recurrent wheeze later in childhood. It has been proposed that RSV infection stimulates type-2 cytokine responses, resembling those found in atopy and asthma. Peripheral blood cells were obtained from RSV-infected infants (n ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Bendelja K,Gagro A,Bace A,Lokar-Kolbas R,Krsulovic-Hresic V,Drazenovic V,Mlinaric-Galinovic G,Rabatic S

    更新日期:2000-08-01 00:00:00

  • Mixed lymphocyte reaction and graft survival in forty cadaveric renal transplants.

    abstract::The value of the MLR in predicting graft outcome was evaluated in forty cadaveric grafts. The MLR was assessed by the one and two way stimulation index (SI), the relative response (RR), and the stabilized relative response (SRR). Graft function was assessed by function at 3 and 6 months after transplantation, and a re...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Cullen PR,Lester S,Rouch J,Morris PJ

    更新日期:1977-05-01 00:00:00

  • The role of neutrophil activation in determining the outcome of pregnancy and modulation by hormones and/or cytokines.

    abstract::Neutrophils are often exclusively considered as a first-line innate immune defence, able to rapidly kill or trap pathogens and causing in case of over-activation tissue damage. In the female reproductive tract, however, the presence and activity of neutrophils seems to be tightly regulated. Major players in orchestrat...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,评审


    authors: Hahn S,Hasler P,Vokalova L,van Breda SV,Lapaire O,Than NG,Hoesli I,Rossi SW

    更新日期:2019-10-01 00:00:00

  • Monocytes from patients with rheumatoid arthritis and type 2 diabetes mellitus display an increased production of interleukin (IL)-1β via the nucleotide-binding domain and leucine-rich repeat containing family pyrin 3(NLRP3)-inflammasome activation: a pos

    abstract::A better understanding about the mechanisms involved in the pathogenesis of type 2 diabetes mellitus (T2D) showed that inflammatory cytokines such as tumour necrosis factor (TNF) and interleukin (IL)-1β play a pivotal role, mirroring data largely reported in rheumatoid arthritis (RA). IL-1β is produced mainly by monoc...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Ruscitti P,Cipriani P,Di Benedetto P,Liakouli V,Berardicurti O,Carubbi F,Ciccia F,Alvaro S,Triolo G,Giacomelli R

    更新日期:2015-10-01 00:00:00

  • Interaction of lectin pathway of complement-activating pattern recognition molecules with mycobacteria.

    abstract::We have demonstrated that mannose-binding lectin (MBL) recognizes various slow-growing, pathogenic mycobacteria [Mycobacterium tuberculosis (MTB), M. bovis, M. kansasii, M. gordonae] as well as non-pathogenic M. smegmatis. Recognition resulted in activation of the lectin pathway (LP) of complement and an enhancement o...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Bartlomiejczyk MA,Swierzko AS,Brzostek A,Dziadek J,Cedzynski M

    更新日期:2014-11-01 00:00:00

  • HLA class II glycoprotein expression by monocytes from HIV-infected individuals: differences in response to interferon-gamma.

    abstract::The expression of HLA class II glycoproteins by monocytes from HIV-infected individuals was evaluated. We found no differences in HLA-DR, HLA-DQ, or HLA-DP expression by freshly isolated monocytes from patients with clinical AIDS, HIV-infected patients without AIDS or from uninfected individuals. However, monocytes fr...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Zwilling BS,Darrow R,Mcpeek M,Laufman H,Pearl D

    更新日期:1990-12-01 00:00:00

  • Antigen-presenting capacity of macrophages and dendritic cells in the peritoneal cavity of patients treated with peritoneal dialysis.

    abstract::In this study the antigen-presenting capacity of human peritoneal cells and the influence of continuous ambulant peritoneal dialysis (CAPD) were studied. On average 6% of the peritoneal cells were dendritic cells (DC), with no difference between CAPD and control peritoneal cells. DC were enriched by selecting for non-...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Betjes MG,Tuk CW,Struijk DG,Krediet RT,Arisz L,Beelen RH

    更新日期:1993-11-01 00:00:00

  • Characterization of immunological depression in mice exposed to normobaric oxygen.

    abstract::Immunological cell functions were evaluated during 24, 48 and 96 h O2 exposure in C57Bl/6 mice. A normobaric O2 exposure resulted in depression of delayed type hypersensitivity (DTH) to oxazolone and Staphylococcus aureus antigens. This effect was proportional to the duration of O2 exposure. The antibody response of s...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Levacher-Place M,Gougerot-Pocidalo MA,Rouveix B,Kraus L,Pocidalo JJ

    更新日期:1983-11-01 00:00:00

  • Unresponsiveness to Con A in spleen cell cultures of M. lepraemurium-infected mice is dependent on a defective expression of high-affinity IL-2 receptors rather than on a lack of IL-2 production.

    abstract::The production of interleukin-2 (IL-2) by Con A-activated spleen cells (SC) progressively declined and reached negligible values during the course of infection of C57BL/6 mice with Mycobacterium lepraemurium. In addition, the capacity of cultured SC to utilize IL-2 was highly reduced, as demonstrated by the accumulati...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Turcotte R,Lemieux S

    更新日期:1989-04-01 00:00:00

  • IgG subclass distribution of antibody responses to protein and polysaccharide mycobacterial antigens in leprosy and tuberculosis patients.

    abstract::Immunoenzymatic assays were developed for the measurement of antibodies against mycobacterial lipoarabinomannan (LAM), a cell-free proteic extract (CFX) of Mycobacterium leprae, and the 38-kD protein antigen of M. tuberculosis. Sera from 108 leprosy patients, belonging to all clinical-immunological forms of the spectr...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Sousa AO,Henry S,Marója FM,Lee FK,Brum L,Singh M,Lagrange PH,Aucouturier P

    更新日期:1998-01-01 00:00:00

  • Demonstration of 11S IgA antibody to spermatozoa in human seminal fluid.

    abstract::Spontaneous sperm agglutination was observed in the ejaculate of a 39-year-old male (patient Z). Using an enzyme-linked immunosorbent assay (ELISA) with spermatozoa fixed to wells of a microtitre plate it was determined that IgA was associated with patient Z's spermatozoa but not with a pooled sample of spermatozoa fr...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Witkin SS,Zelikovsky G,Good RA,Day NK

    更新日期:1981-05-01 00:00:00

  • Specific antibody-dependent killing of Toxoplasma gondii by normal macrophages.

    abstract::The requirement for specificity of antibody-dependent inhibition or killing of intracellular Toxoplasma gondii trophozoites by normal mouse peritoneal macrophages was evaluated in vitro using light microscopy and autoradiography. Anti-toxoplasma antibody in the presence of 'accessory factor' rendered extracellular T. ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Anderson SE Jr,Bautista SC,Remington JS

    更新日期:1976-12-01 00:00:00

  • Cytotoxic T lymphocyte and natural killer cell responses to non-typeable Haemophilus influenzae.

    abstract::Cytotoxic T lymphocytes (CTL) and natural killer (NK) cells have a key role in host defence against infectious pathogens, but their response to bacteria is not well characterized. Non-typeable Haemophilus influenzae is a major cause of respiratory tract infection including otitis media, sinusitis, tonsillitis and chro...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: King PT,Ngui J,Farmer MW,Hutchinson P,Holmes PW,Holdsworth SR

    更新日期:2008-06-01 00:00:00

  • Renal damage and death in weaned mice after oral infection with Shiga toxin 2-producing Escherichia coli strains.

    abstract::Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 infections are considered a public health problem in both developed and developing countries because of their increasing incidence and the severity of clinical presentation. Approximately 10% of infected patients develop complications such as haemolytic uraemic syndro...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Brando RJ,Miliwebsky E,Bentancor L,Deza N,Baschkier A,Ramos MV,Fernández GC,Meiss R,Rivas M,Palermo MS

    更新日期:2008-08-01 00:00:00

  • Antilactoferrin antibodies in autoimmune liver disease.

    abstract::Antilactoferrin antibodies have been reported in patients with several autoimmune disorders, including primary biliary cirrhosis, autoimmune hepatitis and autoimmune cholangitis. We investigated the prevalence and the clinical significance of such autoreactivity in patients with autoimmune and viral chronic liver dise...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Muratori L,Muratori P,Zauli D,Grassi A,Pappas G,Rodrigo L,Cassani F,Lenzi M,Bianchi FB

    更新日期:2001-06-01 00:00:00

  • Mammalian Toll-like receptors: to immunity and beyond.

    abstract::Toll-like receptors (TLRs) constitute an archetypal pattern recognition system. Their sophisticated biology underpins the ability of innate immunity to discriminate between highly diverse microbial pathogens and self. However, the remarkable progress made in describing this biology has also revealed new immunological ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,评审


    authors: Hopkins PA,Sriskandan S

    更新日期:2005-06-01 00:00:00

  • Characterization of cold reactive lymphocytotoxic antibodies in malaria.

    abstract::Characterization of cold reactive lymphocytotoxic antibodies present in sera from Thai adults with malaria revealed that the antibodies are predominantly 19S (IgM), directed against both autologous and allogeneic mononuclear cells, complement-dependent, present in titres ranging from 1:2 to 1:16, and exhibit greater l...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Gilbreath MJ,Pavanand K,MacDermott RP,Wells RA,Ussery MA

    更新日期:1983-02-01 00:00:00

  • Pre-neutralization of C5a-mediated effects by the monoclonal antibody 137-26 reacting with the C5a moiety of native C5 without preventing C5 cleavage.

    abstract::Complement C5a is aetiologically linked to inflammatory tissue damage in conditions like septicaemia, immune complex diseases and ischaemia-reperfusion injury. We here describe a monoclonal antibody (mAb), 137-26, that binds to the C5a moiety of human C5 and neutralizes the effects of C5a without interfering with C5 c...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Fung M,Lu M,Fure H,Sun W,Sun C,Shi NY,Dou Y,Su J,Swanson X,Mollnes TE

    更新日期:2003-08-01 00:00:00

  • Immunoglobulin (Ig)M antibodies against oxidized cardiolipin but not native cardiolipin are novel biomarkers in haemodialysis patients, associated negatively with mortality.

    abstract::The risk of premature death is high in haemodialysis (HD) patients. Antibodies against cardiolipin (anti-CL) are thrombogenic in diseases such as systemic lupus erythematosus (SLE). CL is easily oxidized (Ox) and plays a role in apoptosis. In this work we studied immunoglobulin (Ig)M anti-CL and anti-OxCL in HD-patien...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Frostegård AG,Hua X,Su J,Carrero JJ,Heimbürger O,Bárány P,Stenvinkel P,Frostegård J

    更新日期:2013-12-01 00:00:00

  • Up-regulation of ICAM-1, CD11a/CD18 and CD11c/CD18 on human THP-1 monocytes stimulated by Streptococcus suis serotype 2.

    abstract::Streptococcus suis serotype 2 is known to be a major pathogen of swine, causing mainly meningitis. It is also a zoonotic agent leading predominantly to meningitis in humans working in close contact with pigs. In this study, we investigated the ability of S. suis to up-regulate the expression of adhesion molecules invo...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Al-Numani D,Segura M,Doré M,Gottschalk M

    更新日期:2003-07-01 00:00:00