Abstract:
:There is an increasing need for novel drugs and new strategies for the therapy of invasive candidiasis. This study aimed to develop and characterize liposome-based nanoparticles of carvacrol, cinnamaldehyde, citral or thymol with anti-Candida activities. Dioctadecyldimethylammonium bromide- and monoolein-based liposomes in a 1:2 molar ratio were prepared using a lipid-film hydration method. Liposomes were assembled with equal volumes of liposomal stock dispersion and stock solutions of carvacrol, cinnamaldehyde, citral or thymol in dimethyl sulfoxide. Cytotoxicity was tested on RAW 264.7 macrophages. In vitro antifungal activity of liposomes with phytocompounds was evaluated according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) methodology using clinical isolates of Candida albicans, Candida auris, Candida dubliniensis and Candida tropicalis Finally, the ability of macrophage cells to kill Candida after the addition of phytocompounds and their nanoparticles was determined. Nanoparticles with 64 μg/ml of cinnamaldehyde, 256 μg/ml of citral and 128 μg/ml of thymol had the best characteristics among the formulations tested. Highest encapsulation efficiencies were achieved with citral (78 to 83%) and carvacrol (66 to 71%) liposomes. Carvacrol and thymol in liposome-based nanoparticles were non-toxic regardless of the concentration. Moreover, carvacrol and thymol maintained their antifungal activity after encapsulation and there was a significant reduction (∼ 41%) of yeast survival when macrophages were incubated with carvacrol or thymol liposomes. In conclusion, carvacrol and thymol liposomes possess high stability, low cytotoxicity, and antifungal activity that acts synergistically with macrophages.
journal_name
Antimicrob Agents Chemotherjournal_title
Antimicrobial agents and chemotherapyauthors
Miranda-Cadena K,Dias M,Costa-Barbosa A,Collins T,Marcos-Arias C,Eraso E,Pais C,Quindós G,Sampaio Pdoi
10.1128/AAC.01628-20subject
Has Abstractpub_date
2021-01-19 00:00:00eissn
0066-4804issn
1098-6596pii
AAC.01628-20pub_type
杂志文章abstract::The promotion of colonization with vancomycin-resistant enterococci (VRE) is one potential side effect during treatment of Clostridium difficile-associated diarrhea (CDAD), resulting from disturbances in gut microbiota. Cadazolid (CDZ) is an investigational antibiotic with potent in vitro activity against C. difficile...
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journal_title:Antimicrobial agents and chemotherapy
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journal_title:Antimicrobial agents and chemotherapy
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journal_title:Antimicrobial agents and chemotherapy
pub_type: 杂志文章
doi:10.1128/AAC.00416-13
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journal_title:Antimicrobial agents and chemotherapy
pub_type: 杂志文章
doi:10.1128/AAC.01462-06
更新日期:2007-12-01 00:00:00
abstract::Placental transfer of the HIV protease inhibitor darunavir was investigated in 5 term human cotyledons perfused with darunavir (1,000 ng/ml) in the maternal to fetal direction. The mean (± the standard deviation [SD]) fetal transfer rate (FTR) (fetal/maternal concentration at steady state from 30 to 90 min) was 15.0%±...
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