Abstract：
BACKGROUND:The MERS-CoV was identified for the first time from Jeddah, Saudi Arabia in 2012 from a hospitalized patient. This virus has now been spread to 27 countries with a total of 858 deaths and 2494 confirmed cases and has become a serious concern for the human population. Camels are well known for the transmission of the virus to the human population. METHODS:In this report, we have discussed the designing, prediction, and evaluation of potential siRNAs against the orf1ab gene of MERS-CoV. The online software was used to predict and design the siRNAs and finally, total twenty-one siRNA were filtered out from four hundred and sixty-two sIRNAs as per their scoring and specificity criteria. We have used only ten siRNAs to evaluate their cytotoxicity and efficacy by reverse transfection approach in HEK-293-T cell lines. RESULTS:Based on the results and data generated; no cytotoxicity was observed for any siRNAs at various concentrations in HEK-293-T cells. The ct value of real-time PCR showed the inhibition of viral replication in siRNA-1, 2, 4, 6, and 9. The data generated provided the preliminary information and encouraged us to evaluate the remaining siRNAs separately as well as in combination to analyses the replication of MERS-CoV inhibition in other cell lines. CONCLUSION:Based on the results obtained; it is concluded that the prediction of siRNAs using online software resulted in the filtration of potential siRNAs with high accuracy and strength. This technology can be used to design and develop antiviral therapy not only for MERS-CoV but also against other viruses.
journal_name
J Infect Public Healthjournal_title
Journal of infection and public healthauthors
Sohrab SS,El-Kafrawy SA,Mirza Z,Hassan AM,Alsaqaf F,Azhar EIdoi
10.1016/j.jiph.2020.12.018subject
Has Abstractpub_date
2020-12-29 00:00:00pages
238-243issue
2eissn
1876-0341issn
1876-035Xpii
S1876-0341(20)30774-7journal_volume
14pub_type
杂志文章abstract：:Myiasis-the feeding of fly larvae on living mammals, may have various clinical presentations depending on the tissues or organs involved. Myiasis is a common travel associated skin disorder as a consequence of short visits to developing countries. It is the fourth most common travel associated disease. The most common...
journal_title：Journal of infection and public health
pub_type： 杂志文章
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abstract：PURPOSE:To determine the rate of device-associated healthcare-associated infections (DA-HAIs) at a respiratory intensive care unit (RICU) and in the pediatric intensive care units (PICUs) of member hospitals of the International Nosocomial Infection Control Consortium (INICC) in Egypt. MATERIALS AND METHODS:A prospect...
journal_title：Journal of infection and public health
pub_type： 杂志文章,多中心研究
doi：10.1016/j.jiph.2012.07.002
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abstract：:The aim of our study was to describe the distribution of Visceral Leishmaniasis (VL) in Italy, focusing on HIV-infected patients, to estimate the burden of the disease and the public health actions that should be undertaken. A review of official notifications and hospitalization data has been performed. From 2006 to 2...
journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2013.03.004
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abstract：:Brucellosis is a common zoonotic disease throughout the world. Brucella spp. transmit to humans through contact with fluids of infected animals, especially sheep, cattle, and goats. It is also transmitted by ingestion of fluid-derived products of infected animals, such as unpasteurized milk and cheese. Brucella spp. c...
journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2014.07.004
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abstract：:Although rare, infective endocarditis (IE) continues to cause significant morbidity and mortality. Previous data from the American University of Beirut Medical Center (AUBMC) had shown predominance of streptococcal infection. As worldwide studies in developed countries show increasing trends in Staphylococcus aureus e...
journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2016.11.017
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abstract：:We report the case of a 10-year-old girl who presented to the emergency department with acute abdominal pain. She was diagnosed as having extrapulmonary tuberculosis (TB) with multifocal osteomyelitis of the spine and ribs, peritonitis and intestinal involvement. We describe the clinical presentation of this unusual c...
journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2011.11.005
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abstract：:This study was designed to explore baseline data about the prevalence and distribution of human papillomavirus (HPV) genotypes among Chinese women who had cervical intraepithelial lesions and cervical cancer. A total of 511 patients were recruited, and biopsy samples were collected from these patients. Polymerase chai...
journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2017.06.012
更新日期：2018-01-01 00:00:00
abstract：:Dengue hemorrhagic fever is transmitted through a bite by a dengue -infected Aedes aegypti mosquito. It was first reported in the mid -20th century in Thailand, and since then its epidemiology has been of great concern and has spread all across the country. The alarming incidence of dengue posed a serious threat to hu...
journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2017.01.016
更新日期：2017-01-01 00:00:00
abstract：:HPV is the sexually transmitted agent most common among young people, like college students. The aim of study was to associate sexual behavior characteristics of women with HPV, detected in genital samples taken before the introduction of the HPV vaccine. Female students during 2001-2005 donated genital samples and th...
journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2017.08.014
更新日期：2018-01-01 00:00:00
abstract：OBJECTIVE:The objective of this study was to evaluate the microbial quality of the well water used as a drinking source in urban and rural households. METHODS:A total of 80 household well water samples were analyzed by the multiple fermentation tube method to determine the presumptive coliform count/most probable numb...
journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2012.03.004
更新日期：2012-06-01 00:00:00
abstract：BACKGROUND:COVID-19 (Coronavirus Disease-2019) is a pandemic disease, infecting more than 26.5 million people. Since there is no specific and effective treatment; early diagnosis and optimal isolation of the patient are of vital importance. Real-time polymerase chain reaction-based (RT-PCR) analyses do not achieve suff...
journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2020.09.009
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abstract：:We conducted a cross-sectional survey to determine the prevalence of the human immunodeficiency virus (HIV) among pregnant women attending a major hospital in Kassala state, eastern Sudan. Unlinked anonymous testing of residual blood specimens, which were originally collected for other routine clinical purposes, was p...
journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2010.12.001
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abstract：:We report a case of Listeria meningitis related to mantle cell lymphoma. A clinical pharmacist adjusted repeatedly the patient's anti-infective therapeutic regimen by analyzing the pharmacologic and pharmacokinetic characteristics of antibacterial drugs (such as cefotaxime, meropenem, etc.) due to the patient's repeat...
journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2019.04.010
更新日期：2019-01-01 00:00:00
abstract：BACKGROUND:Hepatitis B virus (HBV) infection is a well-recognized occupational risk for all healthcare workers (HCWs) worldwide. AIM:This study aimed to assess the knowledge, attitudes, and practices of primary healthcare (PHC) physicians regarding the occupational risks of HBV. METHOD:In this cross-sectional study, ...
journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2013.08.004
更新日期：2014-07-01 00:00:00
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journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2013.02.006
更新日期：2013-08-01 00:00:00
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journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2016.09.021
更新日期：2017-01-01 00:00:00
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journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2012.03.005
更新日期：2012-08-01 00:00:00
abstract：OBJECTIVE:MBL acts as a binding protein that enables uptake of mycobacteria into macrophages. And, TNF-alpha is an important cytokine that is involved in control of mycobacterial infections both in-vivo and in-vitro. A large number of genetic factors exerting susceptibility to tuberculosis has been identified, among wh...
journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2016.11.003
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abstract：:Crack cocaine users represent a target group for hepatitis B vaccination. We evaluate the HBV epidemiology, immunization status and compliance with a super-accelerated vaccination schedule among in-treatment crack cocaine users in central Brazil. Six hundred in-treatment crack cocaine users were interviewed, and serum...
journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2016.02.010
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abstract：BACKGROUND:Due to the increasing availability of antiretroviral treatment, HIV infected children are living longer which points out the necessity of disclosure. And which is important to support the uptake and adherence to treatment. Disclosure is an important measure to prevent secondary transmission when children are...
journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2019.05.018
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abstract：OBJECTIVE:In this study, expression of cancer stem cells (CSCs)-related factor-Sex-determining region of Y chromosome-related high-mobility-group box 2 (SOX2) and anti-apoptotic specific factor- Survivin in salivary adenoid cystic carcinoma (SACC) was detected to provide important clues for effective SACC prevention an...
journal_title：Journal of infection and public health
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journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2020.05.003
更新日期：2020-06-01 00:00:00
abstract：:Staphylococcus lugdunensis produces a tannase with activity that may be associated with the onset of colon carcinoma. To clarify this feature of colon carcinoma-associated S. lugdunensis, we obtained isolates from healthy subjects and patients with colon adenomas and carcinomas and analyzed their genetic backgrounds. ...
journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2017.03.012
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abstract：INTRODUCTION:Carbapenemase-producing Enterobacteriaceae (CPE) are of global concern due to the growing number of patients who acquire them and their association with high mortality rates. Although there are some reports of endemicity in developing countries, little is known about this microorganism, and Ecuador is not ...
journal_title：Journal of infection and public health
pub_type： 杂志文章,多中心研究
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journal_title：Journal of infection and public health
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abstract：BACKGROUND:Cytomegalovirus (CMV) is a rare cause of prolonged febrile neutropenia (PFN) among pediatric oncology patients, especially in non-stem cell transplant setting (Non-SCTS). Infectious Diseases Society of America (IDSA) guidelines stated briefly that neutropenia is not considered as an indication of CMV re-acti...
journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2020.02.001
更新日期：2020-08-01 00:00:00
abstract：INTRODUCTION:Middle East respiratory syndrome coronavirus (MERS-CoV) belongs to the family Coronaviridae, and is named for the crown-like spikes on its surface. The clinical presentation of MERS-CoV infection ranges from asymptomatic to very severe disease, and the classical presentation includes fever, cough chills, s...
journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2018.02.001
更新日期：2018-01-01 00:00:00
abstract：:Brucellosis is an ancient disease that still remains a significant threat to humans and is typically linked to exposure to infected animals and/or consumption of unpasteurized animal products. Despite this history, we have a relatively limited understanding of the host characteristics of this disease; consequently, fu...
journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2012.03.006
更新日期：2012-10-01 00:00:00
abstract：OBJECTIVES:Incidence of tuberculosis (TB) is greatest among patients with impaired immunity. India is experiencing a double epidemic of HIV and diabetes mellitus (DM), both of which are strongly associated with immuno-suppression. This study aimed to discover the prevalence of HIV and DM in both the pulmonary and extra...
journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2011.03.005
更新日期：2011-08-01 00:00:00
abstract：BACKGROUND AND AIMS:Prevalence of hepatitis C virus (HCV) in intravenous drug users (IDU) varies in different areas according to socioeconomic and geographical circumstances. The present study was performed to determine seroprevalence of HCV in IDU individuals in Ahvaz, Iran. MATERIALS AND METHODS:142 IDU patients wer...
journal_title：Journal of infection and public health
pub_type： 杂志文章
doi：10.1016/j.jiph.2009.01.001
更新日期：2009-01-01 00:00:00
