Abstract：
:Glutamine (gln) metabolism has emerged as a cancer therapeutic target in past few years, however, the effect of gln-deprivation of bCSCs remains elusive in breast cancer. In this study, effect of glutamine on stemness and differentiation potential of bCSCs isolated from MCF-7 and MDAMB-231 were studied. We have shown that bCSCs differentiate into CD24+ epithelial population under gln-deprivation and demonstrated increased expression of epithelial markers such as e-cadherin, claudin-1 and decreased expression of mesenchymal protein n-cadherin. MCF-7-bCSCs showed a decrease in EpCAMhigh population whereas MDAMB-231-bCSCs increased CD44high population in response to gln-deprivation. The expression of intracellular stem cell markers such sox-2, oct-4 and nanog showed a drastic decrease in gene expression under gln-deprived MDAMB-231-bCSCs. Finally, localization of β-catenin in MCF-7 and MDAMB-231 cells showed its accumulation in cytosol or perinuclear space reducing its efficiency to transcribe downstream genes. Conclusively, our study demonstrated that gln-deprivation induces differentiation of bCSCs into epithelial subtypes and also reduces stemness of bCSCs mediated by reduced nuclear localization of β-catenin. It also suggests that basal and luminal bCSCs respond differentially towards changes in extracellular and intracellular gln. This study could significantly affect the gln targeting regimen of breast cancer therapeutics.
journal_name
J Cell Commun Signaljournal_title
Journal of cell communication and signalingauthors
Jariyal H,Gupta C,Andhale S,Gadge S,Srivastava Adoi
10.1007/s12079-020-00603-1subject
Has Abstractpub_date
2021-01-28 00:00:00eissn
1873-9601issn
1873-961Xpii
10.1007/s12079-020-00603-1pub_type
杂志文章abstract：:Transforming growth factor beta (TGF-beta) and connective tissue growth factor (CTGF) have been described to induce the production of extracellular matrix (ECM) proteins and have been reported to be increased in different fibrotic disorders. Skeletal muscle fibrosis is a common feature of Duchenne muscular dystrophy (...
journal_title：Journal of cell communication and signaling
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journal_title：Journal of cell communication and signaling
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journal_title：Journal of cell communication and signaling
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doi：10.1007/s12079-018-0490-2
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journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-012-0185-z
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journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-015-0281-y
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journal_title：Journal of cell communication and signaling
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journal_title：Journal of cell communication and signaling
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journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-013-0210-x
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journal_title：Journal of cell communication and signaling
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journal_title：Journal of cell communication and signaling
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journal_title：Journal of cell communication and signaling
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doi：10.1007/s12079-011-0156-9
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journal_title：Journal of cell communication and signaling
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journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-009-0084-0
更新日期：2010-03-01 00:00:00
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journal_title：Journal of cell communication and signaling
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doi：10.1007/s12079-017-0389-3
更新日期：2017-12-01 00:00:00
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journal_title：Journal of cell communication and signaling
pub_type： 杂志文章,评审
doi：10.1007/s12079-018-0492-0
更新日期：2019-09-01 00:00:00
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journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-012-0183-1
更新日期：2013-03-01 00:00:00
abstract：:Erlotinib is an oral tyrosine kinase inhibitor of epidermal growth factor receptor (EGFR) pathway. Although our previous study has proved the efficacy of Erlotinib in head and neck squamous cell carcinoma (HNSCC), it has also demonstrated poor clinical response rates and disappointing results in clinical trials for HN...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-020-00546-7
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journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-017-0432-4
更新日期：2018-03-01 00:00:00
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journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-017-0421-7
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journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-017-0439-x
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journal_title：Journal of cell communication and signaling
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doi：10.1007/s12079-019-00526-6
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journal_title：Journal of cell communication and signaling
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doi：10.1007/s12079-008-0021-7
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journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
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journal_title：Journal of cell communication and signaling
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journal_title：Journal of cell communication and signaling
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journal_title：Journal of cell communication and signaling
pub_type： 杂志文章,评审
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journal_title：Journal of cell communication and signaling
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pub_type： 杂志文章
doi：10.1007/s12079-014-0220-3
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abstract：:Lung immaturity is the major cause of morbidity and mortality in premature infants, especially those born <28 weeks of gestation. These infants are at high risk of developing respiratory distress syndrome (RDS), a lung disease caused by insufficient surfactant production and immaturity of saccular/alveolar type II epi...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-017-0443-1
更新日期：2018-03-01 00:00:00
abstract：:Research over the past few years has highlighted the ability of the unfolded protein response (UPR) to minimize the deleterious effects of accumulated misfolded proteins under both physiological and pathological conditions. The endoplasmic reticulum (ER) adapts to endogenous and exogenous stressors by expanding its pr...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-014-0251-9
更新日期：2014-12-01 00:00:00
