Identification of rat osteoclasts in bone smears with quantification of acid phosphatase activity in vitamin D deficiency.

Abstract:

:Vitamin D deficiency may depress bone formation but its effect on bone resorption is not well defined. As an index of bone resorption, the activity of acid phosphatase, a lysosomal enzyme found in osteoclasts, was quantitated in situ from a bone tissue smear preparation. Activity of the enzyme, measured by integrative microdensitometry increased linearly from 25 to 60 min. The distribution of activity in osteoclasts quantitated appeared to follow a normal distribution with a median value of 0.19 integrated optical density units. Animals treated with vitamin D or 1,25 dihydroxycholecalciferol had significantly increased activity of acid phosphatase in osteoclasts compared to animals which were vitamin D deficient. The increase of acid phosphatase activity averaged 63%. Vitamin D or its metabolites may have a permissive effect on the action of parathyroid hormone or act directly to increase bone resorption.

journal_name

Bone

journal_title

Bone

authors

Silverton SF,Kaye M

doi

10.1016/8756-3282(87)90171-2

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

241-4

issue

4

eissn

8756-3282

issn

1873-2763

pii

8756-3282(87)90171-2

journal_volume

8

pub_type

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