Abstract:
:Fusion proteins known to activate transcription in vivo were tested for the ability to stimulate transcription in vitro in a recently developed Saccharomyces cerevisiae RNA polymerase II transcription system. One fusion protein, whose activation domain was derived from the herpesvirus transcriptional activator VP16, gave more than 100-fold stimulation in the in vitro system. The order of effects of the various proteins was the same for transcription in vitro and in vivo, suggesting that the natural mechanism of activation is preserved in vitro.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Chasman DI,Leatherwood J,Carey M,Ptashne M,Kornberg RDdoi
10.1128/mcb.9.11.4746subject
Has Abstractpub_date
1989-11-01 00:00:00pages
4746-9issue
11eissn
0270-7306issn
1098-5549journal_volume
9pub_type
杂志文章abstract::Recent advancements in genetic and proteomic technologies have revealed that more of the genome encodes proteins than originally thought possible. Specifically, some putative long noncoding RNAs (lncRNAs) have been misannotated as noncoding. Numerous lncRNAs have been found to contain short open reading frames (sORFs)...
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更新日期:2006-09-01 00:00:00
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pub_type: 杂志文章
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